Q641Q2 (FA21A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WASH complex subunit FAM21A

Gene names

Name:

FAM21A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1341 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes By similarity.
Subunit structure	Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via N-terminus) with WASH1; the interaction is direct By similarity.
Subcellular location	Early endosome membrane By similarity.
Miscellaneous	In human, FAM21 has undergone evolutionary duplication, with 4 highly homologous family members existing, including FAM21A, FAM21B, FAM21C, and an N-terminally truncated FAM21D form.
Sequence similarities	Belongs to the FAM21 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Endosome Membrane
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	retrograde transport, endosome to Golgi Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	WASH complex Inferred from sequence or structural similarity. Source: UniProtKB early endosome Inferred from sequence or structural similarity. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q641Q2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q641Q2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     938-958: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1341	1341	WASH complex subunit FAM21A		PRO_0000317299
Natural variations
	Alternative sequence	938 – 958	21	Missing in isoform 2.		VSP_030948
Experimental info
	Sequence conflict	444	1	G → E in AAH75815. Ref.2
	Sequence conflict	816	1	I → T in AAH75815. Ref.2
	Sequence conflict	997	1	H → Y in AAH82258. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 8F0F755EB1493EA7	FASTA	1,341	147,184
	10 20 30 40 50 60 MMNRTTPDQE LAPASEPVWE RPWSVEEIRR SSQSWSLAAD AGLLQFLQEF SQQTISRTHE 70 80 90 100 110 120 IKKQVDGLIR ETKATDCRLH NVFNDFLMLS NTQFIENRVY DEEVEEPVLK AEAEKTEQEK 130 140 150 160 170 180 TREQKEVDLI PKVQEAVNYG LQVLDSAFEQ LDIKAGNSDS EEDDANGRVE LILEPKDLYI 190 200 210 220 230 240 DRPLPYLIGS KLFMEQEDVG LGELSSEEGS VGSDRGSIVD TEEEKEEEES DEDFAHHSDN 250 260 270 280 290 300 EQNRHTTQMS DEEEDDDGCD LFADSEKEEE DIEDIEENTR PKRSRPTSFA DELAARIKGD 310 320 330 340 350 360 AVGRVDEEPT TLPSGEAKPR KTLKEKKERR TPSDDEEDNL FAPPKLTDED FSPFGSGGGL 370 380 390 400 410 420 FSGGKGLFDD EDEESDLFTE APQDRQAGAS VKEESSSSKP GKKIPAGAVS VFLGDTDVFG 430 440 450 460 470 480 AASVPSMKEP QKPEQPTPRK SPYGPPPTGL FDDDDGDDDD DFFSAPHSKP SKTGKVQSTA 490 500 510 520 530 540 DIFGDEEGDL FKEKAVASPE ATVSQTDENK ARAEKKVTLS SSKNLKPSSE TKTQKGLFSD 550 560 570 580 590 600 EEDSEDLFSS QSASKLKGAS LLPGKLPTLV SLFDDEDEED NLFGGTAAKK QTLCLQAQRE 610 620 630 640 650 660 EKAKASELSK KKASALLFSS DEEDQWNIPA SQTHLASDSR SKGEPRDSGT LQSQEAKAVK 670 680 690 700 710 720 KTSLFEEDEE DDLFAIAKDS QKKTQRVSLL FEDDVDSGGS LFGSPPTSVP PATKKKETVS 730 740 750 760 770 780 EAPPLLFSDE EEKEAQLGVK SVDKKVESAK ESLKFGRTDV AESEKEGLLT RSAQETVKHS 790 800 810 820 830 840 DLFSSSSPWD KGTKPRTKTV LSLFDEEEDK MEDQNIIQAP QKEVGKGRDP DAHPKSTGVF 850 860 870 880 890 900 QDEELLFSHK LQKDNDPDVD LFAGTKKTKL LEPSVGSLFG DDEDDDLFSS AKSQPLVQEK 910 920 930 940 950 960 KRVVKKDHSV DSFKNQKHPE SIQGSKEKGI WKPETPQDSS GLAPFKTKEP STRIGKIQAN 970 980 990 1000 1010 1020 LAINPAALLP TAASQISEVK PVLPELAFPS SEHRRSHGLE SVPVLPGSGE AGVSFDLPAQ 1030 1040 1050 1060 1070 1080 ADTLHSANKS RVKMRGKRRP QTRAARRLAA QESSETEDMS VPRGPIAQWA DGAISPNGHR 1090 1100 1110 1120 1130 1140 PQLRAASGED STEEALAAAA APWEGGPVPG VDRSPFAKSL GHSRGEADLF DSGDIFSTGT 1150 1160 1170 1180 1190 1200 GSQSVERTKP KAKIAENPAN PPVGGKAKSP MFPALGEASS DDDLFQSAKP KPAKKTNPFP 1210 1220 1230 1240 1250 1260 LLEDEDDLFT DQKVKKNETK SNSQQDVILT TQDIFEDDIF ATEAIKPSQK TREKEKTLES 1270 1280 1290 1300 1310 1320 NLFDDNIDIF ADLTVKPKEK SKKKVEAKSI FDDDMDDIFS SGIQAKTTKP KSRSAQAAPE 1330 1340 PRFEHKVSNI FDDPLNAFGG Q
	Isoform 2 [UniParc]. Checksum: 64417D36898CFFBF	FASTA	1,320	144,941
	10 20 30 40 50 60 MMNRTTPDQE LAPASEPVWE RPWSVEEIRR SSQSWSLAAD AGLLQFLQEF SQQTISRTHE 70 80 90 100 110 120 IKKQVDGLIR ETKATDCRLH NVFNDFLMLS NTQFIENRVY DEEVEEPVLK AEAEKTEQEK 130 140 150 160 170 180 TREQKEVDLI PKVQEAVNYG LQVLDSAFEQ LDIKAGNSDS EEDDANGRVE LILEPKDLYI 190 200 210 220 230 240 DRPLPYLIGS KLFMEQEDVG LGELSSEEGS VGSDRGSIVD TEEEKEEEES DEDFAHHSDN 250 260 270 280 290 300 EQNRHTTQMS DEEEDDDGCD LFADSEKEEE DIEDIEENTR PKRSRPTSFA DELAARIKGD 310 320 330 340 350 360 AVGRVDEEPT TLPSGEAKPR KTLKEKKERR TPSDDEEDNL FAPPKLTDED FSPFGSGGGL 370 380 390 400 410 420 FSGGKGLFDD EDEESDLFTE APQDRQAGAS VKEESSSSKP GKKIPAGAVS VFLGDTDVFG 430 440 450 460 470 480 AASVPSMKEP QKPEQPTPRK SPYGPPPTGL FDDDDGDDDD DFFSAPHSKP SKTGKVQSTA 490 500 510 520 530 540 DIFGDEEGDL FKEKAVASPE ATVSQTDENK ARAEKKVTLS SSKNLKPSSE TKTQKGLFSD 550 560 570 580 590 600 EEDSEDLFSS QSASKLKGAS LLPGKLPTLV SLFDDEDEED NLFGGTAAKK QTLCLQAQRE 610 620 630 640 650 660 EKAKASELSK KKASALLFSS DEEDQWNIPA SQTHLASDSR SKGEPRDSGT LQSQEAKAVK 670 680 690 700 710 720 KTSLFEEDEE DDLFAIAKDS QKKTQRVSLL FEDDVDSGGS LFGSPPTSVP PATKKKETVS 730 740 750 760 770 780 EAPPLLFSDE EEKEAQLGVK SVDKKVESAK ESLKFGRTDV AESEKEGLLT RSAQETVKHS 790 800 810 820 830 840 DLFSSSSPWD KGTKPRTKTV LSLFDEEEDK MEDQNIIQAP QKEVGKGRDP DAHPKSTGVF 850 860 870 880 890 900 QDEELLFSHK LQKDNDPDVD LFAGTKKTKL LEPSVGSLFG DDEDDDLFSS AKSQPLVQEK 910 920 930 940 950 960 KRVVKKDHSV DSFKNQKHPE SIQGSKEKGI WKPETPQANL AINPAALLPT AASQISEVKP 970 980 990 1000 1010 1020 VLPELAFPSS EHRRSHGLES VPVLPGSGEA GVSFDLPAQA DTLHSANKSR VKMRGKRRPQ 1030 1040 1050 1060 1070 1080 TRAARRLAAQ ESSETEDMSV PRGPIAQWAD GAISPNGHRP QLRAASGEDS TEEALAAAAA 1090 1100 1110 1120 1130 1140 PWEGGPVPGV DRSPFAKSLG HSRGEADLFD SGDIFSTGTG SQSVERTKPK AKIAENPANP 1150 1160 1170 1180 1190 1200 PVGGKAKSPM FPALGEASSD DDLFQSAKPK PAKKTNPFPL LEDEDDLFTD QKVKKNETKS 1210 1220 1230 1240 1250 1260 NSQQDVILTT QDIFEDDIFA TEAIKPSQKT REKEKTLESN LFDDNIDIFA DLTVKPKEKS 1270 1280 1290 1300 1310 1320 KKKVEAKSIF DDDMDDIFSS GIQAKTTKPK SRSAQAAPEP RFEHKVSNIF DDPLNAFGGQ

References

[1]

"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL442003, AL450382 Genomic DNA. Translation: CAM17004.1. AL450382, AL442003 Genomic DNA. Translation: CAM22317.1. BC075815 mRNA. Translation: AAH75815.1. BC082258 mRNA. Translation: AAH82258.2.
IPI	IPI00792422. IPI00797872.
RefSeq	NP_001005751.1. NM_001005751.1.
UniGene	Hs.645272.
3D structure databases
ProteinModelPortal	Q641Q2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000282633.
Polymorphism databases
DMDM	166971555.
Proteomic databases
PaxDb	Q641Q2.
PRIDE	Q641Q2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282633; ENSP00000282633; ENSG00000099290. ENST00000351071; ENSP00000344037; ENSG00000099290.
GeneID	387680.
KEGG	hsa:387680.
UCSC	uc001jjb.3. human. uc010qhi.2. human.
Organism-specific databases
CTD	387680.
GeneCards	GC10P051827.
H-InvDB	HIX0008825.
HGNC	HGNC:23416. FAM21A.
HPA	HPA047844.
neXtProt	NX_Q641Q2.
PharmGKB	PA134902481.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG76301.
HOGENOM	HOG000112469.
HOVERGEN	HBG055529.
InParanoid	Q641Q2.
OMA	VRGKRRP.
Gene expression databases
ArrayExpress	Q641Q2.
Bgee	Q641Q2.
CleanEx	HS_FAM21A.
Genevestigator	Q641Q2.
Family and domain databases
InterPro	IPR027308. FAM21. [Graphical view]
PANTHER	PTHR21669:SF4. PTHR21669:SF4. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	387680.
NextBio	101504.

Entry information

Entry name

FA21A_HUMAN

Accession

Primary (citable) accession number: Q641Q2
Secondary accession number(s): A2A3S2, A2A3U6, Q6DHY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.