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Q641Q2 (FA21A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WASH complex subunit FAM21A
Gene names
Name:FAM21A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1341 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes By similarity.

Subunit structure

Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via N-terminus) with WASH1; the interaction is direct By similarity.

Subcellular location

Early endosome membrane By similarity.

Miscellaneous

In human, FAM21 has undergone evolutionary duplication, with 4 highly homologous family members existing, including FAM21A, FAM21B, FAM21C, and an N-terminally truncated FAM21D form.

Sequence similarities

Belongs to the FAM21 family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentEndosome
Membrane
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processretrograde transport, endosome to Golgi

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentWASH complex

Inferred from sequence or structural similarity. Source: UniProtKB

early endosome

Inferred from sequence or structural similarity. Source: UniProtKB

early endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q641Q2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q641Q2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     938-958: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13411341WASH complex subunit FAM21A
PRO_0000317299

Natural variations

Alternative sequence938 – 95821Missing in isoform 2.
VSP_030948

Experimental info

Sequence conflict4441G → E in AAH75815. Ref.2
Sequence conflict8161I → T in AAH75815. Ref.2
Sequence conflict9971H → Y in AAH82258. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 5, 2008. Version 3.
Checksum: 8F0F755EB1493EA7

FASTA1,341147,184
        10         20         30         40         50         60 
MMNRTTPDQE LAPASEPVWE RPWSVEEIRR SSQSWSLAAD AGLLQFLQEF SQQTISRTHE 

        70         80         90        100        110        120 
IKKQVDGLIR ETKATDCRLH NVFNDFLMLS NTQFIENRVY DEEVEEPVLK AEAEKTEQEK 

       130        140        150        160        170        180 
TREQKEVDLI PKVQEAVNYG LQVLDSAFEQ LDIKAGNSDS EEDDANGRVE LILEPKDLYI 

       190        200        210        220        230        240 
DRPLPYLIGS KLFMEQEDVG LGELSSEEGS VGSDRGSIVD TEEEKEEEES DEDFAHHSDN 

       250        260        270        280        290        300 
EQNRHTTQMS DEEEDDDGCD LFADSEKEEE DIEDIEENTR PKRSRPTSFA DELAARIKGD 

       310        320        330        340        350        360 
AVGRVDEEPT TLPSGEAKPR KTLKEKKERR TPSDDEEDNL FAPPKLTDED FSPFGSGGGL 

       370        380        390        400        410        420 
FSGGKGLFDD EDEESDLFTE APQDRQAGAS VKEESSSSKP GKKIPAGAVS VFLGDTDVFG 

       430        440        450        460        470        480 
AASVPSMKEP QKPEQPTPRK SPYGPPPTGL FDDDDGDDDD DFFSAPHSKP SKTGKVQSTA 

       490        500        510        520        530        540 
DIFGDEEGDL FKEKAVASPE ATVSQTDENK ARAEKKVTLS SSKNLKPSSE TKTQKGLFSD 

       550        560        570        580        590        600 
EEDSEDLFSS QSASKLKGAS LLPGKLPTLV SLFDDEDEED NLFGGTAAKK QTLCLQAQRE 

       610        620        630        640        650        660 
EKAKASELSK KKASALLFSS DEEDQWNIPA SQTHLASDSR SKGEPRDSGT LQSQEAKAVK 

       670        680        690        700        710        720 
KTSLFEEDEE DDLFAIAKDS QKKTQRVSLL FEDDVDSGGS LFGSPPTSVP PATKKKETVS 

       730        740        750        760        770        780 
EAPPLLFSDE EEKEAQLGVK SVDKKVESAK ESLKFGRTDV AESEKEGLLT RSAQETVKHS 

       790        800        810        820        830        840 
DLFSSSSPWD KGTKPRTKTV LSLFDEEEDK MEDQNIIQAP QKEVGKGRDP DAHPKSTGVF 

       850        860        870        880        890        900 
QDEELLFSHK LQKDNDPDVD LFAGTKKTKL LEPSVGSLFG DDEDDDLFSS AKSQPLVQEK 

       910        920        930        940        950        960 
KRVVKKDHSV DSFKNQKHPE SIQGSKEKGI WKPETPQDSS GLAPFKTKEP STRIGKIQAN 

       970        980        990       1000       1010       1020 
LAINPAALLP TAASQISEVK PVLPELAFPS SEHRRSHGLE SVPVLPGSGE AGVSFDLPAQ 

      1030       1040       1050       1060       1070       1080 
ADTLHSANKS RVKMRGKRRP QTRAARRLAA QESSETEDMS VPRGPIAQWA DGAISPNGHR 

      1090       1100       1110       1120       1130       1140 
PQLRAASGED STEEALAAAA APWEGGPVPG VDRSPFAKSL GHSRGEADLF DSGDIFSTGT 

      1150       1160       1170       1180       1190       1200 
GSQSVERTKP KAKIAENPAN PPVGGKAKSP MFPALGEASS DDDLFQSAKP KPAKKTNPFP 

      1210       1220       1230       1240       1250       1260 
LLEDEDDLFT DQKVKKNETK SNSQQDVILT TQDIFEDDIF ATEAIKPSQK TREKEKTLES 

      1270       1280       1290       1300       1310       1320 
NLFDDNIDIF ADLTVKPKEK SKKKVEAKSI FDDDMDDIFS SGIQAKTTKP KSRSAQAAPE 

      1330       1340 
PRFEHKVSNI FDDPLNAFGG Q 

« Hide

Isoform 2 [UniParc].

Checksum: 64417D36898CFFBF
Show »

FASTA1,320144,941

References

[1]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Eye and Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL442003, AL450382 Genomic DNA. Translation: CAM17004.1.
AL450382, AL442003 Genomic DNA. Translation: CAM22317.1.
BC075815 mRNA. Translation: AAH75815.1.
BC082258 mRNA. Translation: AAH82258.2.
CCDSCCDS41527.1. [Q641Q2-1]
RefSeqNP_001005751.1. NM_001005751.2. [Q641Q2-1]
NP_001278327.1. NM_001291398.1. [Q641Q2-2]
UniGeneHs.365286.
Hs.449662.
Hs.645272.

3D structure databases

ProteinModelPortalQ641Q2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid132390. 9 interactions.
STRING9606.ENSP00000282633.

Polymorphism databases

DMDM166971555.

Proteomic databases

MaxQBQ641Q2.
PaxDbQ641Q2.
PRIDEQ641Q2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000282633; ENSP00000282633; ENSG00000099290. [Q641Q2-1]
ENST00000351071; ENSP00000344037; ENSG00000099290. [Q641Q2-2]
ENST00000606580; ENSP00000475905; ENSG00000272064. [Q641Q2-1]
ENST00000606663; ENSP00000475601; ENSG00000272064. [Q641Q2-2]
GeneID387680.
KEGGhsa:387680.
UCSCuc001jjb.3. human. [Q641Q2-1]
uc010qhi.2. human. [Q641Q2-2]

Organism-specific databases

CTD387680.
GeneCardsGC10P051827.
H-InvDBHIX0008825.
HGNCHGNC:23416. FAM21A.
HPAHPA047844.
neXtProtNX_Q641Q2.
PharmGKBPA134902481.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG76301.
HOGENOMHOG000112469.
HOVERGENHBG055529.
InParanoidQ641Q2.
OMAHSDNDQN.
OrthoDBEOG7GBFWP.
PhylomeDBQ641Q2.
TreeFamTF329309.

Gene expression databases

BgeeQ641Q2.
CleanExHS_FAM21A.
GenevestigatorQ641Q2.

Family and domain databases

InterProIPR027308. FAM21.
IPR029341. FAM21/CAPZIP.
[Graphical view]
PANTHERPTHR21669:SF4. PTHR21669:SF4. 1 hit.
PfamPF15255. CAP-ZIP_m. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi387680.
NextBio101504.
PROQ641Q2.

Entry information

Entry nameFA21A_HUMAN
AccessionPrimary (citable) accession number: Q641Q2
Secondary accession number(s): A2A3S2, A2A3U6, Q6DHY0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: July 9, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM