Poly(A) RNA polymerase GLD2-A - Xenopus laevis (African clawed frog)

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Q641A1 (GLD2A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 11, 2012. Version 36. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Poly(A) RNA polymerase GLD2-A
EC=2.7.7.19

Alternative name(s):
PAP-associated domain-containing protein 4-A

Gene names

Name:	papd4-a
Synonyms:	gld2-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity. Ref.3
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1). Ref.3
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbfox2 and sympk. Ref.2 Ref.3
Subcellular location	Cytoplasm Ref.3.
Developmental stage	Present in oocytes. Ref.3
Sequence similarities	Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. Contains 1 PAP-associated domain.

Ontologies

Keywords
Biological process	Differentiation Oogenesis mRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Developmental protein Transferase
Gene Ontology (GO)
Biological_process	RNA polyadenylation Inferred from sequence or structural similarity. Source: UniProtKB cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 509

509

Poly(A) RNA polymerase GLD2-A

PRO_0000341553

Regions

Domain

409 – 462

PAP-associated

Sites

Metal binding

240

Magnesium or manganese; catalytic By similarity

Metal binding

242

Magnesium or manganese; catalytic By similarity

Experimental info

Mutagenesis

242

D → A: Loss of activity. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q641A1 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: F97C98C4BF287EF6
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FASTA

509

57,982

        10         20         30         40         50         60 
MYPNSPSLGR IPLPLPCEQQ QQASGYSDKL PVSAAPELLS PEQFIQASLN IQKHANLSRM 

        70         80         90        100        110        120 
LMNGNVLTVP PVSSPPWAYL NHSPLISPGS PSSSFQNRKR RSDEGNVSYD VKRQRFHSPQ 

       130        140        150        160        170        180 
EQTVNHQAVP LRGDLRCSYP GSPAFPLLQS PSPPVLKEHV SNSGDCWLYD HIDTTLPVAE 

       190        200        210        220        230        240 
DKLSQQILDL FQALQQQVCD IKKKDICRAE LQREIQQIFP QSRLYLVGSS LNGFGTRISD 

       250        260        270        280        290        300 
ADLCLVLKEE PMNQHTEATQ ILGLLHKLFY TRLSYIERLQ FIRAKVPIVK FRDKVSGAEF 

       310        320        330        340        350        360 
DLNVNNVVGI RNTFLLRTYA YLESRVRPLV LVIKKWANHH GINDASRGTL SSYTLVLMVL 

       370        380        390        400        410        420 
HYLQTLPEPI LPSLQKKYPE CFDLSMQLNL VHHAPRNIPP YLSKNETPLG DLLLGFLKYF 

       430        440        450        460        470        480 
AVEFDWSKDI ISVREGKALP RSDDYLWRNK YICVEEPFDG TNTARAVYER QKFDMIRAEF 

       490        500 
LKAWGALRDD RDLYSLLPVT AIVKKMNSL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Oocyte.
[2]	"Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation." Barnard D.C., Ryan K., Manley J.L., Richter J.D. Cell 119:641-651(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SYMPK.
[3]	"Vertebrate GLD2 poly(A) polymerases in the germline and the brain." Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M. RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CPEB1 AND CPSF1, MUTAGENESIS OF ASP-242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082438 mRNA. Translation: AAH82438.1.
RefSeq	NP_001087892.1. NM_001094423.1.
UniGene	Xl.1354.
3D structure databases
ProteinModelPortal	Q641A1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	447753.
KEGG	xla:447753.
Organism-specific databases
CTD	447753.
Xenbase	XB-GENE-1217415. papd4.
Phylogenomic databases
HOVERGEN	HBG097533.
KO	K14079.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. IPR002058. PAP_assoc. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF03828. PAP_assoc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLD2A_XENLA

Accession

Primary (citable) accession number: Q641A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 25, 2004
Last modified:	July 11, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents