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Q641A1

- GLD2A_XENLA

UniProt

Q641A1 - GLD2A_XENLA

Protein

Poly(A) RNA polymerase GLD2-A

Gene

papd4-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi240 – 2401Magnesium or manganese; catalyticBy similarity
    Metal bindingi242 – 2421Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. oogenesis Source: UniProtKB-KW
    4. RNA polyadenylation Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Transferase

    Keywords - Biological processi

    Differentiation, mRNA processing, Oogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase GLD2-A (EC:2.7.7.19)
    Alternative name(s):
    PAP-associated domain-containing protein 4-A
    Gene namesi
    Name:papd4-a
    Synonyms:gld2-a
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-1217415. papd4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi242 – 2421D → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509Poly(A) RNA polymerase GLD2-APRO_0000341553Add
    BLAST

    Expressioni

    Developmental stagei

    Present in oocytes.1 Publication

    Interactioni

    Subunit structurei

    Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbfox2 and sympk.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ641A1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini409 – 46254PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    HOVERGENiHBG097533.
    KOiK14079.

    Family and domain databases

    InterProiIPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q641A1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYPNSPSLGR IPLPLPCEQQ QQASGYSDKL PVSAAPELLS PEQFIQASLN    50
    IQKHANLSRM LMNGNVLTVP PVSSPPWAYL NHSPLISPGS PSSSFQNRKR 100
    RSDEGNVSYD VKRQRFHSPQ EQTVNHQAVP LRGDLRCSYP GSPAFPLLQS 150
    PSPPVLKEHV SNSGDCWLYD HIDTTLPVAE DKLSQQILDL FQALQQQVCD 200
    IKKKDICRAE LQREIQQIFP QSRLYLVGSS LNGFGTRISD ADLCLVLKEE 250
    PMNQHTEATQ ILGLLHKLFY TRLSYIERLQ FIRAKVPIVK FRDKVSGAEF 300
    DLNVNNVVGI RNTFLLRTYA YLESRVRPLV LVIKKWANHH GINDASRGTL 350
    SSYTLVLMVL HYLQTLPEPI LPSLQKKYPE CFDLSMQLNL VHHAPRNIPP 400
    YLSKNETPLG DLLLGFLKYF AVEFDWSKDI ISVREGKALP RSDDYLWRNK 450
    YICVEEPFDG TNTARAVYER QKFDMIRAEF LKAWGALRDD RDLYSLLPVT 500
    AIVKKMNSL 509
    Length:509
    Mass (Da):57,982
    Last modified:October 25, 2004 - v1
    Checksum:iF97C98C4BF287EF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082438 mRNA. Translation: AAH82438.1.
    RefSeqiNP_001087892.1. NM_001094423.1.
    UniGeneiXl.1354.

    Genome annotation databases

    GeneIDi447753.
    KEGGixla:447753.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082438 mRNA. Translation: AAH82438.1 .
    RefSeqi NP_001087892.1. NM_001094423.1.
    UniGenei Xl.1354.

    3D structure databases

    ProteinModelPortali Q641A1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 447753.
    KEGGi xla:447753.

    Organism-specific databases

    CTDi 447753.
    Xenbasei XB-GENE-1217415. papd4.

    Phylogenomic databases

    HOVERGENi HBG097533.
    KOi K14079.

    Family and domain databases

    InterProi IPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Oocyte.
    2. "Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation."
      Barnard D.C., Ryan K., Manley J.L., Richter J.D.
      Cell 119:641-651(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYMPK.
    3. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
      Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
      RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CPEB1 AND CPSF1, MUTAGENESIS OF ASP-242.

    Entry informationi

    Entry nameiGLD2A_XENLA
    AccessioniPrimary (citable) accession number: Q641A1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3