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Q641A1 (GLD2A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 11, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Poly(A) RNA polymerase GLD2-A

EC=2.7.7.19
Alternative name(s):
PAP-associated domain-containing protein 4-A
Gene names
Name:papd4-a
Synonyms:gld2-a
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity. Ref.3

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1). Ref.3

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbfox2 and sympk. Ref.2 Ref.3

Subcellular location

Cytoplasm Ref.3.

Developmental stage

Present in oocytes. Ref.3

Sequence similarities

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Contains 1 PAP-associated domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Poly(A) RNA polymerase GLD2-A
PRO_0000341553

Regions

Domain409 – 46254PAP-associated

Sites

Metal binding2401Magnesium or manganese; catalytic By similarity
Metal binding2421Magnesium or manganese; catalytic By similarity

Experimental info

Mutagenesis2421D → A: Loss of activity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q641A1 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: F97C98C4BF287EF6

FASTA50957,982
        10         20         30         40         50         60 
MYPNSPSLGR IPLPLPCEQQ QQASGYSDKL PVSAAPELLS PEQFIQASLN IQKHANLSRM 

        70         80         90        100        110        120 
LMNGNVLTVP PVSSPPWAYL NHSPLISPGS PSSSFQNRKR RSDEGNVSYD VKRQRFHSPQ 

       130        140        150        160        170        180 
EQTVNHQAVP LRGDLRCSYP GSPAFPLLQS PSPPVLKEHV SNSGDCWLYD HIDTTLPVAE 

       190        200        210        220        230        240 
DKLSQQILDL FQALQQQVCD IKKKDICRAE LQREIQQIFP QSRLYLVGSS LNGFGTRISD 

       250        260        270        280        290        300 
ADLCLVLKEE PMNQHTEATQ ILGLLHKLFY TRLSYIERLQ FIRAKVPIVK FRDKVSGAEF 

       310        320        330        340        350        360 
DLNVNNVVGI RNTFLLRTYA YLESRVRPLV LVIKKWANHH GINDASRGTL SSYTLVLMVL 

       370        380        390        400        410        420 
HYLQTLPEPI LPSLQKKYPE CFDLSMQLNL VHHAPRNIPP YLSKNETPLG DLLLGFLKYF 

       430        440        450        460        470        480 
AVEFDWSKDI ISVREGKALP RSDDYLWRNK YICVEEPFDG TNTARAVYER QKFDMIRAEF 

       490        500 
LKAWGALRDD RDLYSLLPVT AIVKKMNSL 

« Hide

References

« Hide 'large scale' references
[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Oocyte.
[2]"Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation."
Barnard D.C., Ryan K., Manley J.L., Richter J.D.
Cell 119:641-651(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYMPK.
[3]"Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CPEB1 AND CPSF1, MUTAGENESIS OF ASP-242.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC082438 mRNA. Translation: AAH82438.1.
RefSeqNP_001087892.1. NM_001094423.1.
UniGeneXl.1354.

3D structure databases

ProteinModelPortalQ641A1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID447753.
KEGGxla:447753.

Organism-specific databases

CTD447753.
XenbaseXB-GENE-1217415. papd4.

Phylogenomic databases

HOVERGENHBG097533.
KOK14079.

Family and domain databases

InterProIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLD2A_XENLA
AccessionPrimary (citable) accession number: Q641A1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 25, 2004
Last modified: July 11, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families