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Protein

Poly(A) RNA polymerase GLD2-A

Gene

papd4-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi240 – 2401Magnesium or manganese; catalyticBy similarity
Metal bindingi242 – 2421Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. oogenesis Source: UniProtKB-KW
  4. RNA polyadenylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Differentiation, mRNA processing, Oogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2-A (EC:2.7.7.19)
Alternative name(s):
PAP-associated domain-containing protein 4-A
Gene namesi
Name:papd4-a
Synonyms:gld2-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1217415. papd4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi242 – 2421D → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Poly(A) RNA polymerase GLD2-APRO_0000341553Add
BLAST

Expressioni

Developmental stagei

Present in oocytes.1 Publication

Interactioni

Subunit structurei

Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbfox2 and sympk.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ641A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini409 – 46254PAP-associatedAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

HOVERGENiHBG097533.
KOiK14079.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q641A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPNSPSLGR IPLPLPCEQQ QQASGYSDKL PVSAAPELLS PEQFIQASLN
60 70 80 90 100
IQKHANLSRM LMNGNVLTVP PVSSPPWAYL NHSPLISPGS PSSSFQNRKR
110 120 130 140 150
RSDEGNVSYD VKRQRFHSPQ EQTVNHQAVP LRGDLRCSYP GSPAFPLLQS
160 170 180 190 200
PSPPVLKEHV SNSGDCWLYD HIDTTLPVAE DKLSQQILDL FQALQQQVCD
210 220 230 240 250
IKKKDICRAE LQREIQQIFP QSRLYLVGSS LNGFGTRISD ADLCLVLKEE
260 270 280 290 300
PMNQHTEATQ ILGLLHKLFY TRLSYIERLQ FIRAKVPIVK FRDKVSGAEF
310 320 330 340 350
DLNVNNVVGI RNTFLLRTYA YLESRVRPLV LVIKKWANHH GINDASRGTL
360 370 380 390 400
SSYTLVLMVL HYLQTLPEPI LPSLQKKYPE CFDLSMQLNL VHHAPRNIPP
410 420 430 440 450
YLSKNETPLG DLLLGFLKYF AVEFDWSKDI ISVREGKALP RSDDYLWRNK
460 470 480 490 500
YICVEEPFDG TNTARAVYER QKFDMIRAEF LKAWGALRDD RDLYSLLPVT

AIVKKMNSL
Length:509
Mass (Da):57,982
Last modified:October 24, 2004 - v1
Checksum:iF97C98C4BF287EF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082438 mRNA. Translation: AAH82438.1.
RefSeqiNP_001087892.1. NM_001094423.1.
UniGeneiXl.1354.

Genome annotation databases

GeneIDi447753.
KEGGixla:447753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082438 mRNA. Translation: AAH82438.1.
RefSeqiNP_001087892.1. NM_001094423.1.
UniGeneiXl.1354.

3D structure databases

ProteinModelPortaliQ641A1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi447753.
KEGGixla:447753.

Organism-specific databases

CTDi447753.
XenbaseiXB-GENE-1217415. papd4.

Phylogenomic databases

HOVERGENiHBG097533.
KOiK14079.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Oocyte.
  2. "Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation."
    Barnard D.C., Ryan K., Manley J.L., Richter J.D.
    Cell 119:641-651(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYMPK.
  3. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
    Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
    RNA 11:1117-1130(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CPEB1 AND CPSF1, MUTAGENESIS OF ASP-242.

Entry informationi

Entry nameiGLD2A_XENLA
AccessioniPrimary (citable) accession number: Q641A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 9, 2008
Last sequence update: October 24, 2004
Last modified: January 6, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.