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Protein

Transcription factor Dp-2

Gene

Tfdp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The TFDP2:E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi129 – 21082Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Dp-2
Alternative name(s):
Dp-3
E2F dimerization partner 2
Gene namesi
Name:Tfdp2
Synonyms:Dp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107167. Tfdp2.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 446445Transcription factor Dp-2PRO_0000219478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei24 – 241Phosphoserine; by CDK2Sequence analysisBy similarity
Modified residuei42 – 421Phosphoserine; by CDK2Sequence analysis
Modified residuei122 – 1221PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ64163.
PaxDbiQ64163.
PeptideAtlasiQ64163.
PRIDEiQ64163.

PTM databases

PhosphoSiteiQ64163.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest levels in spleen and heart.

Gene expression databases

CleanExiMM_TFDP2.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with E2F family members. The complex can interact with hypophosphorylated retinoblastoma protein RB1 and related proteins (RBL1 and RBL2) that inhibit the E2F transactivation domain. During the cell cycle, RB becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex rendering E2F transcriptionally active. Interacts with GMCL (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. The complex TFDP2:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target gene promoters and represses its transcriptional activity (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
E2f5Q615022EBI-8077763,EBI-7225685
YwhaeP622596EBI-8077763,EBI-356480

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-24228N.
IntActiQ64163. 3 interactions.
STRINGi10090.ENSMUSP00000128260.

Structurei

3D structure databases

ProteinModelPortaliQ64163.
SMRiQ64163. Positions 129-198, 214-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 8223Interaction with CEBPABy similarityAdd
BLAST
Regioni219 – 29274DimerizationSequence analysisAdd
BLAST
Regioni229 – 26133DCB1Add
BLAST
Regioni274 – 33057DCB2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi103 – 11816Nuclear localization signal1 PublicationAdd
BLAST
Motifi176 – 21035DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi432 – 44615Asp/Glu-rich (acidic; NCB domain)Add
BLAST

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ64163.
PhylomeDBiQ64163.

Family and domain databases

CDDicd14458. DP_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR028314. DP-2.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF5. PTHR12548:SF5. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Alpha (identifier: Q64163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAKNVGLPS TNAKLRGFID QNFSPSKGNI SLVAFPVSST NSPTKILPKT
60 70 80 90 100
LGPINVNVGP QMIISTPQRI ANSGSVLIGN PYTPAPAMVT QTHIAEAAGW
110 120 130 140 150
VPSDRKRARE FIDSDFSESK RSKKGDKNGK GLRHFSMKVC EKVQRKGTTS
160 170 180 190 200
YNEVADELVS EFTNSNNHLA ADSAYDQENI RRRVYDALNV LMAMNIISSL
210 220 230 240 250
PTGKKRNQVD CNSAQECQNL EIEKQRRIER IKQKRAQLQE LLLQQIAFKN
260 270 280 290 300
LVQRNRQNEQ QNQGPPAVNS TIQLPFIIIN TSRKTVIDCS ISSDKFEYLF
310 320 330 340 350
NFDNTFEIHD DIEVLKRMGM SFGLESGKCS LEDLKIARSL VPKALEGYIT
360 370 380 390 400
DISTGPSWLN QGLLLNSTQS VSNLDPTTGA TVPQSSVNQG LCLDAEVALA
410 420 430 440
TGQLPASNSH QSSSAASHFS ESRGETPCSF NDEDEEDEEE DPSSPE
Length:446
Mass (Da):49,098
Last modified:May 30, 2000 - v2
Checksum:i0E6E87AE8B82EE8A
GO
Isoform Beta (identifier: Q64163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.

Show »
Length:369
Mass (Da):40,811
Checksum:i3C198019055FB478
GO
Isoform Gamma (identifier: Q64163-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.
     173-173: S → SQ

Show »
Length:370
Mass (Da):40,939
Checksum:iB76BC25F93256944
GO
Isoform Delta (identifier: Q64163-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:385
Mass (Da):42,751
Checksum:iC76E95880BB3582E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6161Missing in isoform Beta, isoform Gamma and isoform Delta. 1 PublicationVSP_001355Add
BLAST
Alternative sequencei103 – 11816Missing in isoform Beta and isoform Gamma. 1 PublicationVSP_001356Add
BLAST
Alternative sequencei173 – 1731S → SQ in isoform Gamma. 1 PublicationVSP_001357

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA. Translation: AAB35506.2.
CCDSiCCDS23413.1. [Q64163-4]
UniGeneiMm.17977.
Mm.447855.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA. Translation: AAB35506.2.
CCDSiCCDS23413.1. [Q64163-4]
UniGeneiMm.17977.
Mm.447855.

3D structure databases

ProteinModelPortaliQ64163.
SMRiQ64163. Positions 129-198, 214-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24228N.
IntActiQ64163. 3 interactions.
STRINGi10090.ENSMUSP00000128260.

PTM databases

PhosphoSiteiQ64163.

Proteomic databases

MaxQBiQ64163.
PaxDbiQ64163.
PeptideAtlasiQ64163.
PRIDEiQ64163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107167. Tfdp2.

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ64163.
PhylomeDBiQ64163.

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.

Miscellaneous databases

ChiTaRSiTfdp2. mouse.
PROiQ64163.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TFDP2.

Family and domain databases

CDDicd14458. DP_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR028314. DP-2.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF5. PTHR12548:SF5. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTFDP2_MOUSE
AccessioniPrimary (citable) accession number: Q64163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.