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Protein

Transcription factor Dp-2

Gene

Tfdp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The TFDP2:E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi129 – 210Sequence analysisAdd BLAST82

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Dp-2
Alternative name(s):
Dp-3
E2F dimerization partner 2
Gene namesi
Name:Tfdp2
Synonyms:Dp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107167. Tfdp2.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002194782 – 446Transcription factor Dp-2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei24Phosphoserine; by CDK2Sequence analysisBy similarity1
Modified residuei42Phosphoserine; by CDK2Sequence analysis1
Modified residuei122PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ64163.
PeptideAtlasiQ64163.
PRIDEiQ64163.

PTM databases

PhosphoSitePlusiQ64163.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest levels in spleen and heart.

Gene expression databases

CleanExiMM_TFDP2.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with E2F family members. The complex can interact with hypophosphorylated retinoblastoma protein RB1 and related proteins (RBL1 and RBL2) that inhibit the E2F transactivation domain. During the cell cycle, RB becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex rendering E2F transcriptionally active. Interacts with GMCL (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. The complex TFDP2:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target gene promoters and represses its transcriptional activity (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
E2f5Q615022EBI-8077763,EBI-7225685
YwhaeP622596EBI-8077763,EBI-356480

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-24228N.
IntActiQ64163. 3 interactors.
STRINGi10090.ENSMUSP00000128260.

Structurei

3D structure databases

ProteinModelPortaliQ64163.
SMRiQ64163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 82Interaction with CEBPABy similarityAdd BLAST23
Regioni219 – 292DimerizationSequence analysisAdd BLAST74
Regioni229 – 261DCB1Add BLAST33
Regioni274 – 330DCB2Add BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi103 – 118Nuclear localization signal1 PublicationAdd BLAST16
Motifi176 – 210DEF boxAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi432 – 446Asp/Glu-rich (acidic; NCB domain)Add BLAST15

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ64163.
PhylomeDBiQ64163.

Family and domain databases

CDDicd14458. DP_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR028314. DP-2.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF5. PTHR12548:SF5. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Alpha (identifier: Q64163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAKNVGLPS TNAKLRGFID QNFSPSKGNI SLVAFPVSST NSPTKILPKT
60 70 80 90 100
LGPINVNVGP QMIISTPQRI ANSGSVLIGN PYTPAPAMVT QTHIAEAAGW
110 120 130 140 150
VPSDRKRARE FIDSDFSESK RSKKGDKNGK GLRHFSMKVC EKVQRKGTTS
160 170 180 190 200
YNEVADELVS EFTNSNNHLA ADSAYDQENI RRRVYDALNV LMAMNIISSL
210 220 230 240 250
PTGKKRNQVD CNSAQECQNL EIEKQRRIER IKQKRAQLQE LLLQQIAFKN
260 270 280 290 300
LVQRNRQNEQ QNQGPPAVNS TIQLPFIIIN TSRKTVIDCS ISSDKFEYLF
310 320 330 340 350
NFDNTFEIHD DIEVLKRMGM SFGLESGKCS LEDLKIARSL VPKALEGYIT
360 370 380 390 400
DISTGPSWLN QGLLLNSTQS VSNLDPTTGA TVPQSSVNQG LCLDAEVALA
410 420 430 440
TGQLPASNSH QSSSAASHFS ESRGETPCSF NDEDEEDEEE DPSSPE
Length:446
Mass (Da):49,098
Last modified:May 30, 2000 - v2
Checksum:i0E6E87AE8B82EE8A
GO
Isoform Beta (identifier: Q64163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.

Show »
Length:369
Mass (Da):40,811
Checksum:i3C198019055FB478
GO
Isoform Gamma (identifier: Q64163-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.
     173-173: S → SQ

Show »
Length:370
Mass (Da):40,939
Checksum:iB76BC25F93256944
GO
Isoform Delta (identifier: Q64163-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:385
Mass (Da):42,751
Checksum:iC76E95880BB3582E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0013551 – 61Missing in isoform Beta, isoform Gamma and isoform Delta. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_001356103 – 118Missing in isoform Beta and isoform Gamma. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_001357173S → SQ in isoform Gamma. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA. Translation: AAB35506.2.
CCDSiCCDS23413.1. [Q64163-4]
UniGeneiMm.17977.
Mm.447855.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA. Translation: AAB35506.2.
CCDSiCCDS23413.1. [Q64163-4]
UniGeneiMm.17977.
Mm.447855.

3D structure databases

ProteinModelPortaliQ64163.
SMRiQ64163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24228N.
IntActiQ64163. 3 interactors.
STRINGi10090.ENSMUSP00000128260.

PTM databases

PhosphoSitePlusiQ64163.

Proteomic databases

PaxDbiQ64163.
PeptideAtlasiQ64163.
PRIDEiQ64163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107167. Tfdp2.

Phylogenomic databases

eggNOGiKOG2829. Eukaryota.
ENOG410Y9QP. LUCA.
HOGENOMiHOG000030696.
HOVERGENiHBG009894.
InParanoidiQ64163.
PhylomeDBiQ64163.

Enzyme and pathway databases

ReactomeiR-MMU-1538133. G0 and Early G1.

Miscellaneous databases

ChiTaRSiTfdp2. mouse.
PROiQ64163.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TFDP2.

Family and domain databases

CDDicd14458. DP_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR028314. DP-2.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR014889. Transc_factor_DP_C.
IPR015648. Transcrpt_fac_DP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12548. PTHR12548. 1 hit.
PTHR12548:SF5. PTHR12548:SF5. 1 hit.
PfamiPF08781. DP. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.
SMARTiSM01138. DP. 1 hit.
SM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTFDP2_MOUSE
AccessioniPrimary (citable) accession number: Q64163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.