Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor BTF3

Gene

Btf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • protein transport Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:Btf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1202875. Btf3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus

  • Note: The heterodimer with NACA is cytoplasmic.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Transcription factor BTF3PRO_0000213549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301PhosphoserineBy similarity
Modified residuei158 – 1581PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64152.
PaxDbiQ64152.
PRIDEiQ64152.

PTM databases

PhosphoSiteiQ64152.

Expressioni

Gene expression databases

BgeeiQ64152.
CleanExiMM_BTF3.
ExpressionAtlasiQ64152. baseline.
GenevisibleiQ64152. MM.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains (By similarity).By similarity

Protein-protein interaction databases

BioGridi230038. 2 interactions.
DIPiDIP-59968N.
IntActiQ64152. 3 interactions.
MINTiMINT-1851805.
STRINGi10090.ENSMUSP00000022163.

Structurei

3D structure databases

ProteinModelPortaliQ64152.
SMRiQ64152. Positions 95-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 14566NAC-A/BPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi183 – 1864Poly-Asp

Sequence similaritiesi

Belongs to the NAC-beta family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG283773.
GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiQ64152.
KOiK01527.
OMAiDGKAPIA.
OrthoDBiEOG7CVPXQ.
PhylomeDBiQ64152.
TreeFamiTF317546.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64152-1) [UniParc]FASTAAdd to basket

Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRTGAPTQA DSRGRGRARG GWPGAEATPS LPLGGSRGRE SQMKETIMNQ
60 70 80 90 100
EKLAKLQAQV RIGGKGTARR KKKVVHRTAT ADDKKLQFSL KKLGVNNISG
110 120 130 140 150
IEEVNMFTNQ GTVIHFNNPK VQASLAANTF TITGHAETKQ LTEMLPSILN
160 170 180 190 200
QLGADSLTSL RRLAEALPKQ SVDGKAPLAT GEDDDDEVPD LVENFDEASK

NEAN
Length:204
Mass (Da):22,031
Last modified:May 24, 2005 - v3
Checksum:i35D92D331272F961
GO
Isoform 2 (identifier: Q64152-2) [UniParc]FASTAAdd to basket

Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471I → V in S79538 (PubMed:7655515).Curated
Sequence conflicti55 – 551K → R in S79538 (PubMed:7655515).Curated
Sequence conflicti109 – 1091N → S in S79537 (PubMed:7655515).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4242Missing in isoform 2. 1 PublicationVSP_013588Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC008233 mRNA. Translation: AAH08233.1.
BC064010 mRNA. Translation: AAH64010.1.
BC080837 mRNA. Translation: AAH80837.1.
S79538 Genomic DNA. No translation available.
S79537 Genomic DNA. No translation available.
CCDSiCCDS26714.1. [Q64152-1]
CCDS49340.1. [Q64152-2]
RefSeqiNP_001164011.1. NM_001170540.1. [Q64152-2]
NP_663430.2. NM_145455.3. [Q64152-1]
UniGeneiMm.371688.

Genome annotation databases

EnsembliENSMUST00000022163; ENSMUSP00000022163; ENSMUSG00000021660. [Q64152-1]
GeneIDi218490.
KEGGimmu:218490.
UCSCiuc007rou.1. mouse. [Q64152-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC008233 mRNA. Translation: AAH08233.1.
BC064010 mRNA. Translation: AAH64010.1.
BC080837 mRNA. Translation: AAH80837.1.
S79538 Genomic DNA. No translation available.
S79537 Genomic DNA. No translation available.
CCDSiCCDS26714.1. [Q64152-1]
CCDS49340.1. [Q64152-2]
RefSeqiNP_001164011.1. NM_001170540.1. [Q64152-2]
NP_663430.2. NM_145455.3. [Q64152-1]
UniGeneiMm.371688.

3D structure databases

ProteinModelPortaliQ64152.
SMRiQ64152. Positions 95-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230038. 2 interactions.
DIPiDIP-59968N.
IntActiQ64152. 3 interactions.
MINTiMINT-1851805.
STRINGi10090.ENSMUSP00000022163.

PTM databases

PhosphoSiteiQ64152.

Proteomic databases

MaxQBiQ64152.
PaxDbiQ64152.
PRIDEiQ64152.

Protocols and materials databases

DNASUi218490.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022163; ENSMUSP00000022163; ENSMUSG00000021660. [Q64152-1]
GeneIDi218490.
KEGGimmu:218490.
UCSCiuc007rou.1. mouse. [Q64152-1]

Organism-specific databases

CTDi689.
MGIiMGI:1202875. Btf3.

Phylogenomic databases

eggNOGiNOG283773.
GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiQ64152.
KOiK01527.
OMAiDGKAPIA.
OrthoDBiEOG7CVPXQ.
PhylomeDBiQ64152.
TreeFamiTF317546.

Miscellaneous databases

NextBioi376316.
PROiQ64152.
SOURCEiSearch...

Gene expression databases

BgeeiQ64152.
CleanExiMM_BTF3.
ExpressionAtlasiQ64152. baseline.
GenevisibleiQ64152. MM.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N and FVB/N-3.
    Tissue: Jaw and Mammary tumor.
  2. "An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice."
    Deng J.M., Behringer R.R.
    Transgenic Res. 4:264-269(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-65 AND 104-158.
    Tissue: Testis.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiBTF3_MOUSE
AccessioniPrimary (citable) accession number: Q64152
Secondary accession number(s): Q64153, Q6GU63, Q6P3F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: July 22, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.