Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor BTF3

Gene

Btf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription (By similarity).By similarity

GO - Biological processi

  • in utero embryonic development Source: MGI
  • protein transport Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:Btf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1202875 Btf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135491 – 204Transcription factor BTF3Add BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Omega-N-methylarginineCombined sources1
Modified residuei30PhosphoserineBy similarity1
Modified residuei44N6-methyllysineBy similarity1
Modified residuei52N6-methyllysineBy similarity1
Modified residuei158PhosphothreonineBy similarity1
Modified residuei171PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ64152
PeptideAtlasiQ64152
PRIDEiQ64152
TopDownProteomicsiQ64152-2 [Q64152-2]

PTM databases

iPTMnetiQ64152
PhosphoSitePlusiQ64152

Expressioni

Gene expression databases

BgeeiENSMUSG00000021660
CleanExiMM_BTF3
ExpressionAtlasiQ64152 baseline and differential
GenevisibleiQ64152 MM

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains (By similarity).By similarity

Protein-protein interaction databases

BioGridi230038, 2 interactors
DIPiDIP-59968N
IntActiQ64152, 4 interactors
MINTiQ64152
STRINGi10090.ENSMUSP00000022163

Structurei

3D structure databases

ProteinModelPortaliQ64152
SMRiQ64152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 145NAC-A/BPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi183 – 186Poly-Asp4

Sequence similaritiesi

Belongs to the NAC-beta family.Curated

Phylogenomic databases

eggNOGiKOG2240 Eukaryota
ENOG4111JBG LUCA
GeneTreeiENSGT00390000006283
HOGENOMiHOG000261245
HOVERGENiHBG004906
InParanoidiQ64152
KOiK01527
OMAiMKEAIMN
OrthoDBiEOG091G0TSG
PhylomeDBiQ64152
TreeFamiTF317546

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PfamiView protein in Pfam
PF01849 NAC, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64152-1) [UniParc]FASTAAdd to basket
Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRTGAPTQA DSRGRGRARG GWPGAEATPS LPLGGSRGRE SQMKETIMNQ
60 70 80 90 100
EKLAKLQAQV RIGGKGTARR KKKVVHRTAT ADDKKLQFSL KKLGVNNISG
110 120 130 140 150
IEEVNMFTNQ GTVIHFNNPK VQASLAANTF TITGHAETKQ LTEMLPSILN
160 170 180 190 200
QLGADSLTSL RRLAEALPKQ SVDGKAPLAT GEDDDDEVPD LVENFDEASK

NEAN
Length:204
Mass (Da):22,031
Last modified:May 24, 2005 - v3
Checksum:i35D92D331272F961
GO
Isoform 2 (identifier: Q64152-2) [UniParc]FASTAAdd to basket
Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47I → V in S79538 (PubMed:7655515).Curated1
Sequence conflicti55K → R in S79538 (PubMed:7655515).Curated1
Sequence conflicti109N → S in S79537 (PubMed:7655515).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135881 – 42Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC008233 mRNA Translation: AAH08233.1
BC064010 mRNA Translation: AAH64010.1
BC080837 mRNA Translation: AAH80837.1
S79538 Genomic DNA No translation available.
S79537 Genomic DNA No translation available.
CCDSiCCDS26714.1 [Q64152-1]
CCDS49340.1 [Q64152-2]
RefSeqiNP_001164011.1, NM_001170540.1 [Q64152-2]
NP_663430.2, NM_145455.3 [Q64152-1]
UniGeneiMm.371688

Genome annotation databases

EnsembliENSMUST00000022163; ENSMUSP00000022163; ENSMUSG00000021660 [Q64152-1]
ENSMUST00000134542; ENSMUSP00000115500; ENSMUSG00000021660 [Q64152-2]
ENSMUST00000152704; ENSMUSP00000118093; ENSMUSG00000021660 [Q64152-2]
GeneIDi218490
KEGGimmu:218490
UCSCiuc007rou.1 mouse [Q64152-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBTF3_MOUSE
AccessioniPrimary (citable) accession number: Q64152
Secondary accession number(s): Q64153, Q6GU63, Q6P3F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 129 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health