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Q64152

- BTF3_MOUSE

UniProt

Q64152 - BTF3_MOUSE

Protein

Transcription factor BTF3

Gene

Btf3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (24 May 2005)
      Previous versions | rss
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    Functioni

    When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription By similarity.By similarity

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. protein transport Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Transcription, Transcription regulation, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor BTF3
    Alternative name(s):
    Nascent polypeptide-associated complex subunit beta
    Short name:
    NAC-beta
    RNA polymerase B transcription factor 3
    Gene namesi
    Name:Btf3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1202875. Btf3.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus
    Note: The heterodimer with NACA is cytoplasmic.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Transcription factor BTF3PRO_0000213549Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ64152.
    PaxDbiQ64152.
    PRIDEiQ64152.

    PTM databases

    PhosphoSiteiQ64152.

    Expressioni

    Gene expression databases

    BgeeiQ64152.
    CleanExiMM_BTF3.
    GenevestigatoriQ64152.

    Interactioni

    Subunit structurei

    Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230038. 2 interactions.
    DIPiDIP-59968N.
    IntActiQ64152. 3 interactions.
    MINTiMINT-1851805.
    STRINGi10090.ENSMUSP00000022163.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64152.
    SMRiQ64152. Positions 95-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 14566NAC-A/BPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi183 – 1864Poly-Asp

    Sequence similaritiesi

    Belongs to the NAC-beta family.Curated
    Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283773.
    GeneTreeiENSGT00390000006283.
    HOGENOMiHOG000261245.
    HOVERGENiHBG004906.
    InParanoidiQ64152.
    KOiK01527.
    OMAiDGKAPIA.
    OrthoDBiEOG7CVPXQ.
    PhylomeDBiQ64152.
    TreeFamiTF317546.

    Family and domain databases

    InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
    [Graphical view]
    PfamiPF01849. NAC. 1 hit.
    [Graphical view]
    PROSITEiPS51151. NAC_AB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64152-1) [UniParc]FASTAAdd to Basket

    Also known as: BTF3a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRTGAPTQA DSRGRGRARG GWPGAEATPS LPLGGSRGRE SQMKETIMNQ    50
    EKLAKLQAQV RIGGKGTARR KKKVVHRTAT ADDKKLQFSL KKLGVNNISG 100
    IEEVNMFTNQ GTVIHFNNPK VQASLAANTF TITGHAETKQ LTEMLPSILN 150
    QLGADSLTSL RRLAEALPKQ SVDGKAPLAT GEDDDDEVPD LVENFDEASK 200
    NEAN 204
    Length:204
    Mass (Da):22,031
    Last modified:May 24, 2005 - v3
    Checksum:i35D92D331272F961
    GO
    Isoform 2 (identifier: Q64152-2) [UniParc]FASTAAdd to Basket

    Also known as: BTF3b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-42: Missing.

    Show »
    Length:162
    Mass (Da):17,699
    Checksum:i5062DE8858CEE979
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471I → V in S79538. (PubMed:7655515)Curated
    Sequence conflicti55 – 551K → R in S79538. (PubMed:7655515)Curated
    Sequence conflicti109 – 1091N → S in S79537. (PubMed:7655515)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4242Missing in isoform 2. 1 PublicationVSP_013588Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC008233 mRNA. Translation: AAH08233.1.
    BC064010 mRNA. Translation: AAH64010.1.
    BC080837 mRNA. Translation: AAH80837.1.
    S79538 Genomic DNA. No translation available.
    S79537 Genomic DNA. No translation available.
    CCDSiCCDS26714.1. [Q64152-1]
    CCDS49340.1. [Q64152-2]
    RefSeqiNP_001164011.1. NM_001170540.1. [Q64152-2]
    NP_663430.2. NM_145455.3. [Q64152-1]
    UniGeneiMm.371688.

    Genome annotation databases

    EnsembliENSMUST00000022163; ENSMUSP00000022163; ENSMUSG00000021660. [Q64152-1]
    ENSMUST00000134542; ENSMUSP00000115500; ENSMUSG00000021660. [Q64152-2]
    ENSMUST00000152704; ENSMUSP00000118093; ENSMUSG00000021660. [Q64152-2]
    GeneIDi218490.
    KEGGimmu:218490.
    UCSCiuc007rou.1. mouse. [Q64152-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC008233 mRNA. Translation: AAH08233.1 .
    BC064010 mRNA. Translation: AAH64010.1 .
    BC080837 mRNA. Translation: AAH80837.1 .
    S79538 Genomic DNA. No translation available.
    S79537 Genomic DNA. No translation available.
    CCDSi CCDS26714.1. [Q64152-1 ]
    CCDS49340.1. [Q64152-2 ]
    RefSeqi NP_001164011.1. NM_001170540.1. [Q64152-2 ]
    NP_663430.2. NM_145455.3. [Q64152-1 ]
    UniGenei Mm.371688.

    3D structure databases

    ProteinModelPortali Q64152.
    SMRi Q64152. Positions 95-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230038. 2 interactions.
    DIPi DIP-59968N.
    IntActi Q64152. 3 interactions.
    MINTi MINT-1851805.
    STRINGi 10090.ENSMUSP00000022163.

    PTM databases

    PhosphoSitei Q64152.

    Proteomic databases

    MaxQBi Q64152.
    PaxDbi Q64152.
    PRIDEi Q64152.

    Protocols and materials databases

    DNASUi 218490.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022163 ; ENSMUSP00000022163 ; ENSMUSG00000021660 . [Q64152-1 ]
    ENSMUST00000134542 ; ENSMUSP00000115500 ; ENSMUSG00000021660 . [Q64152-2 ]
    ENSMUST00000152704 ; ENSMUSP00000118093 ; ENSMUSG00000021660 . [Q64152-2 ]
    GeneIDi 218490.
    KEGGi mmu:218490.
    UCSCi uc007rou.1. mouse. [Q64152-1 ]

    Organism-specific databases

    CTDi 689.
    MGIi MGI:1202875. Btf3.

    Phylogenomic databases

    eggNOGi NOG283773.
    GeneTreei ENSGT00390000006283.
    HOGENOMi HOG000261245.
    HOVERGENi HBG004906.
    InParanoidi Q64152.
    KOi K01527.
    OMAi DGKAPIA.
    OrthoDBi EOG7CVPXQ.
    PhylomeDBi Q64152.
    TreeFami TF317546.

    Miscellaneous databases

    NextBioi 376316.
    PROi Q64152.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q64152.
    CleanExi MM_BTF3.
    Genevestigatori Q64152.

    Family and domain databases

    InterProi IPR002715. Nas_poly-pep-assoc_cplx_dom.
    [Graphical view ]
    Pfami PF01849. NAC. 1 hit.
    [Graphical view ]
    PROSITEi PS51151. NAC_AB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N and FVB/N-3.
      Tissue: Jaw and Mammary tumor.
    2. "An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice."
      Deng J.M., Behringer R.R.
      Transgenic Res. 4:264-269(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-65 AND 104-158.
      Tissue: Testis.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiBTF3_MOUSE
    AccessioniPrimary (citable) accession number: Q64152
    Secondary accession number(s): Q64153, Q6GU63, Q6P3F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3