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Q64152 (BTF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name=NAC-beta
RNA polymerase B transcription factor 3
Gene names
Name:Btf3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription By similarity.

Subunit structure

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus. Note: The heterodimer with NACA is cytoplasmic By similarity.

Sequence similarities

Belongs to the NAC-beta family.

Contains 1 NAC-A/B (NAC-alpha/beta) domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q64152-1)

Also known as: BTF3a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q64152-2)

Also known as: BTF3b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Transcription factor BTF3
PRO_0000213549

Regions

Domain80 – 14566NAC-A/B
Compositional bias183 – 1864Poly-Asp

Amino acid modifications

Modified residue301Phosphoserine By similarity

Natural variations

Alternative sequence1 – 4242Missing in isoform 2.
VSP_013588

Experimental info

Sequence conflict471I → V in S79538. Ref.2
Sequence conflict551K → R in S79538. Ref.2
Sequence conflict1091N → S in S79537. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (BTF3a) [UniParc].

Last modified May 24, 2005. Version 3.
Checksum: 35D92D331272F961

FASTA20422,031
        10         20         30         40         50         60 
MRRTGAPTQA DSRGRGRARG GWPGAEATPS LPLGGSRGRE SQMKETIMNQ EKLAKLQAQV 

        70         80         90        100        110        120 
RIGGKGTARR KKKVVHRTAT ADDKKLQFSL KKLGVNNISG IEEVNMFTNQ GTVIHFNNPK 

       130        140        150        160        170        180 
VQASLAANTF TITGHAETKQ LTEMLPSILN QLGADSLTSL RRLAEALPKQ SVDGKAPLAT 

       190        200 
GEDDDDEVPD LVENFDEASK NEAN 

« Hide

Isoform 2 (BTF3b) [UniParc].

Checksum: 5062DE8858CEE979
Show »

FASTA16217,699

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N and FVB/N-3.
Tissue: Jaw and Mammary tumor.
[2]"An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice."
Deng J.M., Behringer R.R.
Transgenic Res. 4:264-269(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-65 AND 104-158.
Tissue: Testis.
[3]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC008233 mRNA. Translation: AAH08233.1.
BC064010 mRNA. Translation: AAH64010.1.
BC080837 mRNA. Translation: AAH80837.1.
S79538 Genomic DNA. No translation available.
S79537 Genomic DNA. No translation available.
CCDSCCDS26714.1. [Q64152-1]
CCDS49340.1. [Q64152-2]
RefSeqNP_001164011.1. NM_001170540.1. [Q64152-2]
NP_663430.2. NM_145455.3. [Q64152-1]
UniGeneMm.371688.

3D structure databases

ProteinModelPortalQ64152.
SMRQ64152. Positions 95-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230038. 2 interactions.
DIPDIP-59968N.
IntActQ64152. 3 interactions.
MINTMINT-1851805.
STRING10090.ENSMUSP00000022163.

PTM databases

PhosphoSiteQ64152.

Proteomic databases

MaxQBQ64152.
PaxDbQ64152.
PRIDEQ64152.

Protocols and materials databases

DNASU218490.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022163; ENSMUSP00000022163; ENSMUSG00000021660. [Q64152-1]
ENSMUST00000134542; ENSMUSP00000115500; ENSMUSG00000021660. [Q64152-2]
ENSMUST00000152704; ENSMUSP00000118093; ENSMUSG00000021660. [Q64152-2]
GeneID218490.
KEGGmmu:218490.
UCSCuc007rou.1. mouse. [Q64152-1]

Organism-specific databases

CTD689.
MGIMGI:1202875. Btf3.

Phylogenomic databases

eggNOGNOG283773.
GeneTreeENSGT00390000006283.
HOGENOMHOG000261245.
HOVERGENHBG004906.
InParanoidQ64152.
KOK01527.
OMADGKAPIA.
OrthoDBEOG7CVPXQ.
PhylomeDBQ64152.
TreeFamTF317546.

Gene expression databases

BgeeQ64152.
CleanExMM_BTF3.
GenevestigatorQ64152.

Family and domain databases

InterProIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamPF01849. NAC. 1 hit.
[Graphical view]
PROSITEPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio376316.
PROQ64152.
SOURCESearch...

Entry information

Entry nameBTF3_MOUSE
AccessionPrimary (citable) accession number: Q64152
Secondary accession number(s): Q64153, Q6GU63, Q6P3F3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot