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Q64151

- SEM4C_MOUSE

UniProt

Q64151 - SEM4C_MOUSE

Protein

Semaphorin-4C

Gene

Sema4c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. receptor activity Source: InterPro

    GO - Biological processi

    1. cell migration in hindbrain Source: UniProtKB
    2. cerebellum development Source: UniProtKB
    3. muscle cell differentiation Source: UniProtKB
    4. neural tube closure Source: UniProtKB
    5. positive regulation of stress-activated MAPK cascade Source: UniProtKB
    6. semaphorin-plexin signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Semaphorin-4C
    Alternative name(s):
    M-Sema F
    Semaphorin-C-like 1
    Short name:
    Sema I
    Short name:
    Semaphorin I
    Gene namesi
    Name:Sema4c
    Synonyms:S4c, Semacl1, Semai
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:109252. Sema4c.

    Subcellular locationi

    Cell junctionsynapsepostsynaptic cell membranepostsynaptic density 1 Publication; Single-pass type I membrane protein 1 Publication. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. postsynaptic density Source: UniProtKB
    4. postsynaptic membrane Source: UniProtKB-KW
    5. synaptic vesicle membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Disruption phenotypei

    Partial neonate lethality, due to defects in brain and neural tube development. In about one third of the embryos cranial neural folds fail to converge, leading to an open neural tube and exencephaly. Mice without exencephaly are viable and fertile.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi832 – 8321S → A: Loss of the effect on myogenic differentiation; when associated with A-834. Loss of interaction with DLG4; when associated with A-833 and A-834. 2 Publications
    Mutagenesisi833 – 8331S → A: Loss of interaction with DLG4; when associated with A-832 and A-834. 1 Publication
    Mutagenesisi834 – 8341V → A: Loss of the effect on myogenic differentiation; when associated with A-832. Loss of interaction with DLG4; when associated with A-832 and A-833. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 834814Semaphorin-4CPRO_0000032326Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi99 ↔ 110By similarity
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi128 ↔ 137By similarity
    Disulfide bondi261 ↔ 370By similarity
    Disulfide bondi285 ↔ 330By similarity
    Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi500 ↔ 517By similarity
    Disulfide bondi509 ↔ 526By similarity
    Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi578 ↔ 628By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ64151.

    PTM databases

    PhosphoSiteiQ64151.

    Expressioni

    Tissue specificityi

    Predominantly expressed in brain (at protein level).1 Publication

    Developmental stagei

    First detected at E14, levels increase by birth and remain strong in the adult brain (at protein level). Expressed widely in the nervous tissues during development.1 Publication

    Inductioni

    Up-regulated in differentiating myoblasts and upon muscle regeneration (at protein level).2 Publications

    Gene expression databases

    ArrayExpressiQ64151.
    BgeeiQ64151.
    GenevestigatoriQ64151.

    Interactioni

    Subunit structurei

    Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain). Interacts with NCDN. Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gipc1Q9Z0G08EBI-987075,EBI-300855
    Gipc2Q9Z2H73EBI-987075,EBI-987119

    Protein-protein interaction databases

    IntActiQ64151. 2 interactions.
    MINTiMINT-91862.
    STRINGi10090.ENSMUSP00000027295.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64151.
    SMRiQ64151. Positions 28-633.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 664644ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini686 – 834149CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei665 – 68521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 497468SemaPROSITE-ProRule annotationAdd
    BLAST
    Domaini499 – 55254PSIAdd
    BLAST
    Domaini557 – 64589Ig-like C2-typeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni46 – 489444Dominant negative effect on myogenic differentiationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi831 – 8344PDZ-binding

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi678 – 68710Poly-Leu
    Compositional biasi709 – 77466Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the semaphorin family.Curated
    Contains 1 PSI domain.Curated
    Contains 1 Sema domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG293968.
    GeneTreeiENSGT00560000076864.
    HOGENOMiHOG000116087.
    HOVERGENiHBG061165.
    InParanoidiQ64151.
    KOiK06521.
    OMAiASHLYVC.
    OrthoDBiEOG712TVJ.
    PhylomeDBiQ64151.
    TreeFamiTF316102.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    2.60.40.10. 1 hit.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR016201. Plexin-like_fold.
    IPR002165. Plexin_repeat.
    IPR001627. Semap_dom.
    IPR027231. Semaphorin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PANTHERiPTHR11036. PTHR11036. 1 hit.
    PfamiPF01437. PSI. 1 hit.
    PF01403. Sema. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    SM00423. PSI. 1 hit.
    SM00630. Sema. 1 hit.
    [Graphical view]
    SUPFAMiSSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    PROSITEiPS50835. IG_LIKE. 1 hit.
    PS51004. SEMA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q64151-1 [UniParc]FASTAAdd to Basket

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    MAPHWAVWLL AAGLWGLGIG AEMWWNLVPR KTVSSGELVT VVRRFSQTGI    50
    QDFLTLTLTE HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT 100
    QKGKSNQTEC FNFIRFLQPY NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA 150
    EFEDGKGKCP YDPAKGHTGL LVDGELYSAT LNNFLGTEPV ILRYMGTHHS 200
    IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS ERAVEYDCYS 250
    EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV 300
    HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE 350
    QAQKWARYTD PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM 400
    EDQVKPRLGR PLLVKKNTNF THVVADRVPG LDGATYTVLF IGTGDGWLLK 450
    AVSLGPWIHM VEELQVFDQE PVESLVLSQS KKVLFAGSRS QLVQLSLADC 500
    TKYRFCVDCV LARDPYCAWN VNTSRCVATT SGRSGSFLVQ HVANLDTSKM 550
    CNQYGIKKVR SIPKNITVVS GTDLVLPCHL SSNLAHAHWT FGSQDLPAEQ 600
    PGSFLYDTGL QALVVMAAQS RHSGPYRCYS EEQGTRLAAE SYLVAVVAGS 650
    SVTLEARAPL ENLGLVWLAV VALGAVCLVL LLLVLSLRRR LREELEKGAK 700
    ASERTLVYPL ELPKEPASPP FRPGPETDEK LWDPVGYYYS DGSLKIVPGH 750
    ARCQPGGGPP SPPPGIPGQP LPSPTRLHLG GGRNSNANGY VRLQLGGEDR 800
    GGSGHPLPEL ADELRRKLQQ RQPLPDSNPE ESSV 834
    Length:834
    Mass (Da):92,557
    Last modified:November 1, 1996 - v1
    Checksum:i6868BB5BF571482D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79463 mRNA. Translation: AAB35184.1.
    CCDSiCCDS48240.1.
    PIRiS66498.
    RefSeqiNP_001119519.1. NM_001126047.2.
    XP_006495868.1. XM_006495805.1.
    XP_006495869.1. XM_006495806.1.
    XP_006495870.1. XM_006495807.1.
    XP_006495871.1. XM_006495808.1.
    XP_006495872.1. XM_006495809.1.
    XP_006495873.1. XM_006495810.1.
    UniGeneiMm.29558.

    Genome annotation databases

    EnsembliENSMUST00000027295; ENSMUSP00000027295; ENSMUSG00000026121.
    ENSMUST00000114990; ENSMUSP00000110641; ENSMUSG00000026121.
    ENSMUST00000114991; ENSMUSP00000110643; ENSMUSG00000026121.
    ENSMUST00000114992; ENSMUSP00000110644; ENSMUSG00000026121.
    GeneIDi20353.
    KEGGimmu:20353.
    UCSCiuc007aqq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79463 mRNA. Translation: AAB35184.1 .
    CCDSi CCDS48240.1.
    PIRi S66498.
    RefSeqi NP_001119519.1. NM_001126047.2.
    XP_006495868.1. XM_006495805.1.
    XP_006495869.1. XM_006495806.1.
    XP_006495870.1. XM_006495807.1.
    XP_006495871.1. XM_006495808.1.
    XP_006495872.1. XM_006495809.1.
    XP_006495873.1. XM_006495810.1.
    UniGenei Mm.29558.

    3D structure databases

    ProteinModelPortali Q64151.
    SMRi Q64151. Positions 28-633.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q64151. 2 interactions.
    MINTi MINT-91862.
    STRINGi 10090.ENSMUSP00000027295.

    PTM databases

    PhosphoSitei Q64151.

    Proteomic databases

    PRIDEi Q64151.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027295 ; ENSMUSP00000027295 ; ENSMUSG00000026121 .
    ENSMUST00000114990 ; ENSMUSP00000110641 ; ENSMUSG00000026121 .
    ENSMUST00000114991 ; ENSMUSP00000110643 ; ENSMUSG00000026121 .
    ENSMUST00000114992 ; ENSMUSP00000110644 ; ENSMUSG00000026121 .
    GeneIDi 20353.
    KEGGi mmu:20353.
    UCSCi uc007aqq.2. mouse.

    Organism-specific databases

    CTDi 54910.
    MGIi MGI:109252. Sema4c.

    Phylogenomic databases

    eggNOGi NOG293968.
    GeneTreei ENSGT00560000076864.
    HOGENOMi HOG000116087.
    HOVERGENi HBG061165.
    InParanoidi Q64151.
    KOi K06521.
    OMAi ASHLYVC.
    OrthoDBi EOG712TVJ.
    PhylomeDBi Q64151.
    TreeFami TF316102.

    Miscellaneous databases

    ChiTaRSi SEMA4C. mouse.
    NextBioi 298215.
    PROi Q64151.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64151.
    Bgeei Q64151.
    Genevestigatori Q64151.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    2.60.40.10. 1 hit.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR016201. Plexin-like_fold.
    IPR002165. Plexin_repeat.
    IPR001627. Semap_dom.
    IPR027231. Semaphorin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    PANTHERi PTHR11036. PTHR11036. 1 hit.
    Pfami PF01437. PSI. 1 hit.
    PF01403. Sema. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    SM00423. PSI. 1 hit.
    SM00630. Sema. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    PROSITEi PS50835. IG_LIKE. 1 hit.
    PS51004. SEMA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a member of mouse semaphorin family."
      Inagaki S., Furuyama T., Iwahashi Y.
      FEBS Lett. 370:269-272(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Neonatal brain.
    2. "A PDZ protein regulates the distribution of the transmembrane semaphorin, M-SemF."
      Wang L.-H., Kalb R.G., Strittmatter S.M.
      J. Biol. Chem. 274:14137-14146(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GIPC.
    3. "Semaphorin 4C, a transmembrane semaphorin, associates with a neurite-outgrowth-related protein, SFAP75."
      Ohoka Y., Hirotani M., Sugimoto H., Fujioka S., Furuyama T., Inagaki S.
      Biochem. Biophys. Res. Commun. 280:237-243(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NCDN.
    4. "Sema4c, a transmembrane semaphorin, interacts with a post-synaptic density protein, PSD-95."
      Inagaki S., Ohoka Y., Sugimoto H., Fujioka S., Amazaki M., Kurinami H., Miyazaki N., Tohyama M., Furuyama T.
      J. Biol. Chem. 276:9174-9181(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DLG4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-832; SER-833 AND VAL-834.
    5. "Requirement of the transmembrane semaphorin Sema4C for myogenic differentiation."
      Ko J.-A., Gondo T., Inagaki S., Inui M.
      FEBS Lett. 579:2236-2242(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-832 AND VAL-834, INDUCTION.
    6. "Sema4C participates in myogenic differentiation in vivo and in vitro through the p38 MAPK pathway."
      Wu H., Wang X., Liu S., Wu Y., Zhao T., Chen X., Zhu L., Wu Y., Ding X., Peng X., Yuan J., Wang X., Fan W., Fan M.
      Eur. J. Cell Biol. 86:331-344(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    7. "Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo."
      Deng S., Hirschberg A., Worzfeld T., Penachioni J.Y., Korostylev A., Swiercz J.M., Vodrazka P., Mauti O., Stoeckli E.T., Tamagnone L., Offermanns S., Kuner R.
      J. Neurosci. 27:6333-6347(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PLXNB2.
    8. Cited for: FUNCTION, INTERACTION WITH PLXNB2, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiSEM4C_MOUSE
    AccessioniPrimary (citable) accession number: Q64151
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3