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Protein

Semaphorin-4C

Gene

Sema4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.4 Publications

GO - Molecular functioni

GO - Biological processi

  • cell migration in hindbrain Source: UniProtKB
  • cerebellum development Source: UniProtKB
  • muscle cell differentiation Source: UniProtKB
  • negative chemotaxis Source: GO_Central
  • negative regulation of axon extension involved in axon guidance Source: GO_Central
  • neural crest cell migration Source: GO_Central
  • neural tube closure Source: UniProtKB
  • positive regulation of cell migration Source: GO_Central
  • positive regulation of stress-activated MAPK cascade Source: UniProtKB
  • semaphorin-plexin signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-4C
Alternative name(s):
M-Sema F
Semaphorin-C-like 1
Short name:
Sema I
Short name:
Semaphorin I
Gene namesi
Name:Sema4c
Synonyms:S4c, Semacl1, Semai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:109252. Sema4c.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 664ExtracellularSequence analysisAdd BLAST644
Transmembranei665 – 685HelicalSequence analysisAdd BLAST21
Topological domaini686 – 834CytoplasmicSequence analysisAdd BLAST149

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • extracellular space Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-KW
  • synaptic vesicle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Partial neonate lethality, due to defects in brain and neural tube development. In about one third of the embryos cranial neural folds fail to converge, leading to an open neural tube and exencephaly. Mice without exencephaly are viable and fertile.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi832S → A: Loss of the effect on myogenic differentiation; when associated with A-834. Loss of interaction with DLG4; when associated with A-833 and A-834. 2 Publications1
Mutagenesisi833S → A: Loss of interaction with DLG4; when associated with A-832 and A-834. 1 Publication1
Mutagenesisi834V → A: Loss of the effect on myogenic differentiation; when associated with A-832. Loss of interaction with DLG4; when associated with A-832 and A-833. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003232621 – 834Semaphorin-4CAdd BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi99 ↔ 110By similarity
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Glycosylationi121N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi128 ↔ 137By similarity
Disulfide bondi261 ↔ 370By similarity
Disulfide bondi285 ↔ 330By similarity
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi419N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi500 ↔ 517By similarity
Disulfide bondi509 ↔ 526By similarity
Glycosylationi522N-linked (GlcNAc...)Sequence analysis1
Glycosylationi565N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi578 ↔ 628By similarity
Modified residuei743PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ64151.
PaxDbiQ64151.
PeptideAtlasiQ64151.
PRIDEiQ64151.

PTM databases

iPTMnetiQ64151.
PhosphoSitePlusiQ64151.

Expressioni

Tissue specificityi

Predominantly expressed in brain (at protein level).1 Publication

Developmental stagei

First detected at E14, levels increase by birth and remain strong in the adult brain (at protein level). Expressed widely in the nervous tissues during development.1 Publication

Inductioni

Up-regulated in differentiating myoblasts and upon muscle regeneration (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000026121.
ExpressionAtlasiQ64151. baseline and differential.
GenevisibleiQ64151. MM.

Interactioni

Subunit structurei

Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain). Interacts with NCDN. Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gipc1Q9Z0G08EBI-987075,EBI-300855
Gipc2Q9Z2H73EBI-987075,EBI-987119

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ64151. 2 interactors.
MINTiMINT-91862.
STRINGi10090.ENSMUSP00000027295.

Structurei

3D structure databases

ProteinModelPortaliQ64151.
SMRiQ64151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 497SemaPROSITE-ProRule annotationAdd BLAST468
Domaini499 – 552PSIAdd BLAST54
Domaini557 – 645Ig-like C2-typeAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 489Dominant negative effect on myogenic differentiationAdd BLAST444

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi831 – 834PDZ-binding4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi678 – 687Poly-Leu10
Compositional biasi709 – 774Pro-richAdd BLAST66

Sequence similaritiesi

Belongs to the semaphorin family.Curated
Contains 1 PSI domain.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
GeneTreeiENSGT00760000118854.
HOGENOMiHOG000116087.
HOVERGENiHBG061165.
InParanoidiQ64151.
KOiK06521.
OMAiQPYNASH.
OrthoDBiEOG091G01W0.
PhylomeDBiQ64151.
TreeFamiTF316102.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR002165. Plexin_repeat.
IPR016201. PSI.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPHWAVWLL AAGLWGLGIG AEMWWNLVPR KTVSSGELVT VVRRFSQTGI
60 70 80 90 100
QDFLTLTLTE HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT
110 120 130 140 150
QKGKSNQTEC FNFIRFLQPY NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA
160 170 180 190 200
EFEDGKGKCP YDPAKGHTGL LVDGELYSAT LNNFLGTEPV ILRYMGTHHS
210 220 230 240 250
IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS ERAVEYDCYS
260 270 280 290 300
EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV
310 320 330 340 350
HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE
360 370 380 390 400
QAQKWARYTD PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM
410 420 430 440 450
EDQVKPRLGR PLLVKKNTNF THVVADRVPG LDGATYTVLF IGTGDGWLLK
460 470 480 490 500
AVSLGPWIHM VEELQVFDQE PVESLVLSQS KKVLFAGSRS QLVQLSLADC
510 520 530 540 550
TKYRFCVDCV LARDPYCAWN VNTSRCVATT SGRSGSFLVQ HVANLDTSKM
560 570 580 590 600
CNQYGIKKVR SIPKNITVVS GTDLVLPCHL SSNLAHAHWT FGSQDLPAEQ
610 620 630 640 650
PGSFLYDTGL QALVVMAAQS RHSGPYRCYS EEQGTRLAAE SYLVAVVAGS
660 670 680 690 700
SVTLEARAPL ENLGLVWLAV VALGAVCLVL LLLVLSLRRR LREELEKGAK
710 720 730 740 750
ASERTLVYPL ELPKEPASPP FRPGPETDEK LWDPVGYYYS DGSLKIVPGH
760 770 780 790 800
ARCQPGGGPP SPPPGIPGQP LPSPTRLHLG GGRNSNANGY VRLQLGGEDR
810 820 830
GGSGHPLPEL ADELRRKLQQ RQPLPDSNPE ESSV
Length:834
Mass (Da):92,557
Last modified:November 1, 1996 - v1
Checksum:i6868BB5BF571482D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79463 mRNA. Translation: AAB35184.1.
CCDSiCCDS48240.1.
PIRiS66498.
RefSeqiNP_001119519.1. NM_001126047.3.
NP_001291258.1. NM_001304329.1.
NP_001291259.1. NM_001304330.1.
XP_006495868.1. XM_006495805.2.
XP_006495870.1. XM_006495807.1.
XP_006495871.1. XM_006495808.2.
XP_006495872.1. XM_006495809.3.
XP_006495873.1. XM_006495810.3.
UniGeneiMm.29558.

Genome annotation databases

EnsembliENSMUST00000114991; ENSMUSP00000110643; ENSMUSG00000026121.
ENSMUST00000191642; ENSMUSP00000142284; ENSMUSG00000026121.
ENSMUST00000191677; ENSMUSP00000141263; ENSMUSG00000026121.
ENSMUST00000195620; ENSMUSP00000141527; ENSMUSG00000026121.
GeneIDi20353.
KEGGimmu:20353.
UCSCiuc007aqq.4. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79463 mRNA. Translation: AAB35184.1.
CCDSiCCDS48240.1.
PIRiS66498.
RefSeqiNP_001119519.1. NM_001126047.3.
NP_001291258.1. NM_001304329.1.
NP_001291259.1. NM_001304330.1.
XP_006495868.1. XM_006495805.2.
XP_006495870.1. XM_006495807.1.
XP_006495871.1. XM_006495808.2.
XP_006495872.1. XM_006495809.3.
XP_006495873.1. XM_006495810.3.
UniGeneiMm.29558.

3D structure databases

ProteinModelPortaliQ64151.
SMRiQ64151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64151. 2 interactors.
MINTiMINT-91862.
STRINGi10090.ENSMUSP00000027295.

PTM databases

iPTMnetiQ64151.
PhosphoSitePlusiQ64151.

Proteomic databases

MaxQBiQ64151.
PaxDbiQ64151.
PeptideAtlasiQ64151.
PRIDEiQ64151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114991; ENSMUSP00000110643; ENSMUSG00000026121.
ENSMUST00000191642; ENSMUSP00000142284; ENSMUSG00000026121.
ENSMUST00000191677; ENSMUSP00000141263; ENSMUSG00000026121.
ENSMUST00000195620; ENSMUSP00000141527; ENSMUSG00000026121.
GeneIDi20353.
KEGGimmu:20353.
UCSCiuc007aqq.4. mouse.

Organism-specific databases

CTDi54910.
MGIiMGI:109252. Sema4c.

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
GeneTreeiENSGT00760000118854.
HOGENOMiHOG000116087.
HOVERGENiHBG061165.
InParanoidiQ64151.
KOiK06521.
OMAiQPYNASH.
OrthoDBiEOG091G01W0.
PhylomeDBiQ64151.
TreeFamiTF316102.

Miscellaneous databases

ChiTaRSiSema4c. mouse.
PROiQ64151.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026121.
ExpressionAtlasiQ64151. baseline and differential.
GenevisibleiQ64151. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR002165. Plexin_repeat.
IPR016201. PSI.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEM4C_MOUSE
AccessioniPrimary (citable) accession number: Q64151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.