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Q64151 (SEM4C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Semaphorin-4C
Alternative name(s):
M-Sema F
Semaphorin-C-like 1
Short name=Sema I
Short name=Semaphorin I
Gene names
Name:Sema4c
Synonyms:S4c, Semacl1, Semai
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Ref.5 Ref.6 Ref.7 Ref.8

Subunit structure

Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain). Interacts with NCDN. Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2. Ref.2 Ref.3 Ref.4 Ref.7 Ref.8

Subcellular location

Cell junctionsynapsepostsynaptic cell membranepostsynaptic density; Single-pass type I membrane protein. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass type I membrane protein Ref.4.

Tissue specificity

Predominantly expressed in brain (at protein level). Ref.4

Developmental stage

First detected at E14, levels increase by birth and remain strong in the adult brain (at protein level). Expressed widely in the nervous tissues during development. Ref.4

Induction

Up-regulated in differentiating myoblasts and upon muscle regeneration (at protein level). Ref.5 Ref.6

Disruption phenotype

Partial neonate lethality, due to defects in brain and neural tube development. In about one third of the embryos cranial neural folds fail to converge, leading to an open neural tube and exencephaly. Mice without exencephaly are viable and fertile. Ref.8

Sequence similarities

Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 PSI domain.

Contains 1 Sema domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
   Cellular componentCell junction
Cell membrane
Cytoplasmic vesicle
Membrane
Postsynaptic cell membrane
Synapse
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell migration in hindbrain

Inferred from mutant phenotype Ref.8. Source: UniProtKB

cerebellum development

Inferred from mutant phenotype Ref.8. Source: UniProtKB

muscle cell differentiation

Inferred from mutant phenotype Ref.5Ref.6. Source: UniProtKB

neural tube closure

Inferred from mutant phenotype Ref.8. Source: UniProtKB

positive regulation of stress-activated MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

semaphorin-plexin signaling pathway

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

postsynaptic density

Inferred from direct assay Ref.4. Source: UniProtKB

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-KW

synaptic vesicle membrane

Inferred from direct assay Ref.4. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.4. Source: UniProtKB

receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Gipc1Q9Z0G08EBI-987075,EBI-300855
Gipc2Q9Z2H73EBI-987075,EBI-987119

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 834814Semaphorin-4C
PRO_0000032326

Regions

Topological domain21 – 664644Extracellular Potential
Transmembrane665 – 68521Helical; Potential
Topological domain686 – 834149Cytoplasmic Potential
Domain30 – 497468Sema
Domain499 – 55254PSI
Domain557 – 64589Ig-like C2-type
Region46 – 489444Dominant negative effect on myogenic differentiation
Motif831 – 8344PDZ-binding
Compositional bias678 – 68710Poly-Leu
Compositional bias709 – 77466Pro-rich

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation3101N-linked (GlcNAc...) Potential
Glycosylation4191N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Potential
Glycosylation5651N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 110 By similarity
Disulfide bond128 ↔ 137 By similarity
Disulfide bond261 ↔ 370 By similarity
Disulfide bond285 ↔ 330 By similarity
Disulfide bond500 ↔ 517 By similarity
Disulfide bond509 ↔ 526 By similarity
Disulfide bond578 ↔ 628 By similarity

Experimental info

Mutagenesis8321S → A: Loss of the effect on myogenic differentiation; when associated with A-834. Loss of interaction with DLG4; when associated with A-833 and A-834. Ref.4 Ref.5
Mutagenesis8331S → A: Loss of interaction with DLG4; when associated with A-832 and A-834. Ref.4
Mutagenesis8341V → A: Loss of the effect on myogenic differentiation; when associated with A-832. Loss of interaction with DLG4; when associated with A-832 and A-833. Ref.4 Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q64151 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6868BB5BF571482D

FASTA83492,557
        10         20         30         40         50         60 
MAPHWAVWLL AAGLWGLGIG AEMWWNLVPR KTVSSGELVT VVRRFSQTGI QDFLTLTLTE 

        70         80         90        100        110        120 
HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT QKGKSNQTEC FNFIRFLQPY 

       130        140        150        160        170        180 
NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA EFEDGKGKCP YDPAKGHTGL LVDGELYSAT 

       190        200        210        220        230        240 
LNNFLGTEPV ILRYMGTHHS IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS 

       250        260        270        280        290        300 
ERAVEYDCYS EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV 

       310        320        330        340        350        360 
HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE QAQKWARYTD 

       370        380        390        400        410        420 
PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM EDQVKPRLGR PLLVKKNTNF 

       430        440        450        460        470        480 
THVVADRVPG LDGATYTVLF IGTGDGWLLK AVSLGPWIHM VEELQVFDQE PVESLVLSQS 

       490        500        510        520        530        540 
KKVLFAGSRS QLVQLSLADC TKYRFCVDCV LARDPYCAWN VNTSRCVATT SGRSGSFLVQ 

       550        560        570        580        590        600 
HVANLDTSKM CNQYGIKKVR SIPKNITVVS GTDLVLPCHL SSNLAHAHWT FGSQDLPAEQ 

       610        620        630        640        650        660 
PGSFLYDTGL QALVVMAAQS RHSGPYRCYS EEQGTRLAAE SYLVAVVAGS SVTLEARAPL 

       670        680        690        700        710        720 
ENLGLVWLAV VALGAVCLVL LLLVLSLRRR LREELEKGAK ASERTLVYPL ELPKEPASPP 

       730        740        750        760        770        780 
FRPGPETDEK LWDPVGYYYS DGSLKIVPGH ARCQPGGGPP SPPPGIPGQP LPSPTRLHLG 

       790        800        810        820        830 
GGRNSNANGY VRLQLGGEDR GGSGHPLPEL ADELRRKLQQ RQPLPDSNPE ESSV 

« Hide

References

[1]"Identification of a member of mouse semaphorin family."
Inagaki S., Furuyama T., Iwahashi Y.
FEBS Lett. 370:269-272(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Neonatal brain.
[2]"A PDZ protein regulates the distribution of the transmembrane semaphorin, M-SemF."
Wang L.-H., Kalb R.G., Strittmatter S.M.
J. Biol. Chem. 274:14137-14146(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GIPC.
[3]"Semaphorin 4C, a transmembrane semaphorin, associates with a neurite-outgrowth-related protein, SFAP75."
Ohoka Y., Hirotani M., Sugimoto H., Fujioka S., Furuyama T., Inagaki S.
Biochem. Biophys. Res. Commun. 280:237-243(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NCDN.
[4]"Sema4c, a transmembrane semaphorin, interacts with a post-synaptic density protein, PSD-95."
Inagaki S., Ohoka Y., Sugimoto H., Fujioka S., Amazaki M., Kurinami H., Miyazaki N., Tohyama M., Furuyama T.
J. Biol. Chem. 276:9174-9181(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DLG4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-832; SER-833 AND VAL-834.
[5]"Requirement of the transmembrane semaphorin Sema4C for myogenic differentiation."
Ko J.-A., Gondo T., Inagaki S., Inui M.
FEBS Lett. 579:2236-2242(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF SER-832 AND VAL-834, INDUCTION.
[6]"Sema4C participates in myogenic differentiation in vivo and in vitro through the p38 MAPK pathway."
Wu H., Wang X., Liu S., Wu Y., Zhao T., Chen X., Zhu L., Wu Y., Ding X., Peng X., Yuan J., Wang X., Fan W., Fan M.
Eur. J. Cell Biol. 86:331-344(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[7]"Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo."
Deng S., Hirschberg A., Worzfeld T., Penachioni J.Y., Korostylev A., Swiercz J.M., Vodrazka P., Mauti O., Stoeckli E.T., Tamagnone L., Offermanns S., Kuner R.
J. Neurosci. 27:6333-6347(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PLXNB2.
[8]"Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development."
Maier V., Jolicoeur C., Rayburn H., Takegahara N., Kumanogoh A., Kikutani H., Tessier-Lavigne M., Wurst W., Friedel R.H.
Mol. Cell. Neurosci. 46:419-431(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PLXNB2, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S79463 mRNA. Translation: AAB35184.1.
CCDSCCDS48240.1.
PIRS66498.
RefSeqNP_001119519.1. NM_001126047.2.
XP_006495868.1. XM_006495805.1.
XP_006495869.1. XM_006495806.1.
XP_006495870.1. XM_006495807.1.
XP_006495871.1. XM_006495808.1.
XP_006495872.1. XM_006495809.1.
XP_006495873.1. XM_006495810.1.
UniGeneMm.29558.

3D structure databases

ProteinModelPortalQ64151.
SMRQ64151. Positions 28-633.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ64151. 2 interactions.
MINTMINT-91862.
STRING10090.ENSMUSP00000027295.

PTM databases

PhosphoSiteQ64151.

Proteomic databases

PRIDEQ64151.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027295; ENSMUSP00000027295; ENSMUSG00000026121.
ENSMUST00000114990; ENSMUSP00000110641; ENSMUSG00000026121.
ENSMUST00000114991; ENSMUSP00000110643; ENSMUSG00000026121.
ENSMUST00000114992; ENSMUSP00000110644; ENSMUSG00000026121.
GeneID20353.
KEGGmmu:20353.
UCSCuc007aqq.2. mouse.

Organism-specific databases

CTD54910.
MGIMGI:109252. Sema4c.

Phylogenomic databases

eggNOGNOG293968.
GeneTreeENSGT00560000076864.
HOGENOMHOG000116087.
HOVERGENHBG061165.
InParanoidQ64151.
KOK06521.
OMAASHLYVC.
OrthoDBEOG712TVJ.
PhylomeDBQ64151.
TreeFamTF316102.

Gene expression databases

ArrayExpressQ64151.
BgeeQ64151.
GenevestigatorQ64151.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERPTHR11036. PTHR11036. 1 hit.
PfamPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSEMA4C. mouse.
NextBio298215.
PROQ64151.
SOURCESearch...

Entry information

Entry nameSEM4C_MOUSE
AccessionPrimary (citable) accession number: Q64151
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot