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Q64151

- SEM4C_MOUSE

UniProt

Q64151 - SEM4C_MOUSE

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Protein
Semaphorin-4C
Gene
Sema4c, S4c, Semacl1, Semai
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.4 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. receptor activity Source: InterPro

GO - Biological processi

  1. cell migration in hindbrain Source: UniProtKB
  2. cerebellum development Source: UniProtKB
  3. muscle cell differentiation Source: UniProtKB
  4. neural tube closure Source: UniProtKB
  5. positive regulation of stress-activated MAPK cascade Source: UniProtKB
  6. semaphorin-plexin signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-4C
Alternative name(s):
M-Sema F
Semaphorin-C-like 1
Short name:
Sema I
Short name:
Semaphorin I
Gene namesi
Name:Sema4c
Synonyms:S4c, Semacl1, Semai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:109252. Sema4c.

Subcellular locationi

Cell junctionsynapsepostsynaptic cell membranepostsynaptic density; Single-pass type I membrane protein. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 664644Extracellular Reviewed prediction
Add
BLAST
Transmembranei665 – 68521Helical; Reviewed prediction
Add
BLAST
Topological domaini686 – 834149Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. postsynaptic density Source: UniProtKB
  4. postsynaptic membrane Source: UniProtKB-KW
  5. synaptic vesicle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Partial neonate lethality, due to defects in brain and neural tube development. In about one third of the embryos cranial neural folds fail to converge, leading to an open neural tube and exencephaly. Mice without exencephaly are viable and fertile.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi832 – 8321S → A: Loss of the effect on myogenic differentiation; when associated with A-834. Loss of interaction with DLG4; when associated with A-833 and A-834. 2 Publications
Mutagenesisi833 – 8331S → A: Loss of interaction with DLG4; when associated with A-832 and A-834. 1 Publication
Mutagenesisi834 – 8341V → A: Loss of the effect on myogenic differentiation; when associated with A-832. Loss of interaction with DLG4; when associated with A-832 and A-833. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 834814Semaphorin-4C
PRO_0000032326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi99 ↔ 110 By similarity
Glycosylationi106 – 1061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi121 – 1211N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi128 ↔ 137 By similarity
Disulfide bondi261 ↔ 370 By similarity
Disulfide bondi285 ↔ 330 By similarity
Glycosylationi310 – 3101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi419 – 4191N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi500 ↔ 517 By similarity
Disulfide bondi509 ↔ 526 By similarity
Glycosylationi522 – 5221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi565 – 5651N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi578 ↔ 628 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ64151.

PTM databases

PhosphoSiteiQ64151.

Expressioni

Tissue specificityi

Predominantly expressed in brain (at protein level).1 Publication

Developmental stagei

First detected at E14, levels increase by birth and remain strong in the adult brain (at protein level). Expressed widely in the nervous tissues during development.1 Publication

Inductioni

Up-regulated in differentiating myoblasts and upon muscle regeneration (at protein level).2 Publications

Gene expression databases

ArrayExpressiQ64151.
BgeeiQ64151.
GenevestigatoriQ64151.

Interactioni

Subunit structurei

Interacts (via the PDZ-binding motif) with GIPC (via the PDZ domain). Interacts with NCDN. Interacts (via the PDZ-binding motif) with DLG4. Interacts with PLXNB2.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gipc1Q9Z0G08EBI-987075,EBI-300855
Gipc2Q9Z2H73EBI-987075,EBI-987119

Protein-protein interaction databases

IntActiQ64151. 2 interactions.
MINTiMINT-91862.
STRINGi10090.ENSMUSP00000027295.

Structurei

3D structure databases

ProteinModelPortaliQ64151.
SMRiQ64151. Positions 28-633.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 497468Sema
Add
BLAST
Domaini499 – 55254PSI
Add
BLAST
Domaini557 – 64589Ig-like C2-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni46 – 489444Dominant negative effect on myogenic differentiation
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi831 – 8344PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi678 – 68710Poly-Leu
Compositional biasi709 – 77466Pro-rich
Add
BLAST

Sequence similaritiesi

Belongs to the semaphorin family.
Contains 1 PSI domain.
Contains 1 Sema domain.

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293968.
GeneTreeiENSGT00560000076864.
HOGENOMiHOG000116087.
HOVERGENiHBG061165.
InParanoidiQ64151.
KOiK06521.
OMAiASHLYVC.
OrthoDBiEOG712TVJ.
PhylomeDBiQ64151.
TreeFamiTF316102.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64151-1 [UniParc]FASTAAdd to Basket

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MAPHWAVWLL AAGLWGLGIG AEMWWNLVPR KTVSSGELVT VVRRFSQTGI    50
QDFLTLTLTE HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT 100
QKGKSNQTEC FNFIRFLQPY NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA 150
EFEDGKGKCP YDPAKGHTGL LVDGELYSAT LNNFLGTEPV ILRYMGTHHS 200
IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS ERAVEYDCYS 250
EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV 300
HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE 350
QAQKWARYTD PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM 400
EDQVKPRLGR PLLVKKNTNF THVVADRVPG LDGATYTVLF IGTGDGWLLK 450
AVSLGPWIHM VEELQVFDQE PVESLVLSQS KKVLFAGSRS QLVQLSLADC 500
TKYRFCVDCV LARDPYCAWN VNTSRCVATT SGRSGSFLVQ HVANLDTSKM 550
CNQYGIKKVR SIPKNITVVS GTDLVLPCHL SSNLAHAHWT FGSQDLPAEQ 600
PGSFLYDTGL QALVVMAAQS RHSGPYRCYS EEQGTRLAAE SYLVAVVAGS 650
SVTLEARAPL ENLGLVWLAV VALGAVCLVL LLLVLSLRRR LREELEKGAK 700
ASERTLVYPL ELPKEPASPP FRPGPETDEK LWDPVGYYYS DGSLKIVPGH 750
ARCQPGGGPP SPPPGIPGQP LPSPTRLHLG GGRNSNANGY VRLQLGGEDR 800
GGSGHPLPEL ADELRRKLQQ RQPLPDSNPE ESSV 834
Length:834
Mass (Da):92,557
Last modified:November 1, 1996 - v1
Checksum:i6868BB5BF571482D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S79463 mRNA. Translation: AAB35184.1.
CCDSiCCDS48240.1.
PIRiS66498.
RefSeqiNP_001119519.1. NM_001126047.2.
XP_006495868.1. XM_006495805.1.
XP_006495869.1. XM_006495806.1.
XP_006495870.1. XM_006495807.1.
XP_006495871.1. XM_006495808.1.
XP_006495872.1. XM_006495809.1.
XP_006495873.1. XM_006495810.1.
UniGeneiMm.29558.

Genome annotation databases

EnsembliENSMUST00000027295; ENSMUSP00000027295; ENSMUSG00000026121.
ENSMUST00000114990; ENSMUSP00000110641; ENSMUSG00000026121.
ENSMUST00000114991; ENSMUSP00000110643; ENSMUSG00000026121.
ENSMUST00000114992; ENSMUSP00000110644; ENSMUSG00000026121.
GeneIDi20353.
KEGGimmu:20353.
UCSCiuc007aqq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S79463 mRNA. Translation: AAB35184.1 .
CCDSi CCDS48240.1.
PIRi S66498.
RefSeqi NP_001119519.1. NM_001126047.2.
XP_006495868.1. XM_006495805.1.
XP_006495869.1. XM_006495806.1.
XP_006495870.1. XM_006495807.1.
XP_006495871.1. XM_006495808.1.
XP_006495872.1. XM_006495809.1.
XP_006495873.1. XM_006495810.1.
UniGenei Mm.29558.

3D structure databases

ProteinModelPortali Q64151.
SMRi Q64151. Positions 28-633.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q64151. 2 interactions.
MINTi MINT-91862.
STRINGi 10090.ENSMUSP00000027295.

PTM databases

PhosphoSitei Q64151.

Proteomic databases

PRIDEi Q64151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027295 ; ENSMUSP00000027295 ; ENSMUSG00000026121 .
ENSMUST00000114990 ; ENSMUSP00000110641 ; ENSMUSG00000026121 .
ENSMUST00000114991 ; ENSMUSP00000110643 ; ENSMUSG00000026121 .
ENSMUST00000114992 ; ENSMUSP00000110644 ; ENSMUSG00000026121 .
GeneIDi 20353.
KEGGi mmu:20353.
UCSCi uc007aqq.2. mouse.

Organism-specific databases

CTDi 54910.
MGIi MGI:109252. Sema4c.

Phylogenomic databases

eggNOGi NOG293968.
GeneTreei ENSGT00560000076864.
HOGENOMi HOG000116087.
HOVERGENi HBG061165.
InParanoidi Q64151.
KOi K06521.
OMAi ASHLYVC.
OrthoDBi EOG712TVJ.
PhylomeDBi Q64151.
TreeFami TF316102.

Miscellaneous databases

ChiTaRSi SEMA4C. mouse.
NextBioi 298215.
PROi Q64151.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64151.
Bgeei Q64151.
Genevestigatori Q64151.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
PANTHERi PTHR11036. PTHR11036. 1 hit.
Pfami PF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view ]
SUPFAMi SSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEi PS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a member of mouse semaphorin family."
    Inagaki S., Furuyama T., Iwahashi Y.
    FEBS Lett. 370:269-272(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neonatal brain.
  2. "A PDZ protein regulates the distribution of the transmembrane semaphorin, M-SemF."
    Wang L.-H., Kalb R.G., Strittmatter S.M.
    J. Biol. Chem. 274:14137-14146(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIPC.
  3. "Semaphorin 4C, a transmembrane semaphorin, associates with a neurite-outgrowth-related protein, SFAP75."
    Ohoka Y., Hirotani M., Sugimoto H., Fujioka S., Furuyama T., Inagaki S.
    Biochem. Biophys. Res. Commun. 280:237-243(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCDN.
  4. "Sema4c, a transmembrane semaphorin, interacts with a post-synaptic density protein, PSD-95."
    Inagaki S., Ohoka Y., Sugimoto H., Fujioka S., Amazaki M., Kurinami H., Miyazaki N., Tohyama M., Furuyama T.
    J. Biol. Chem. 276:9174-9181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DLG4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF SER-832; SER-833 AND VAL-834.
  5. "Requirement of the transmembrane semaphorin Sema4C for myogenic differentiation."
    Ko J.-A., Gondo T., Inagaki S., Inui M.
    FEBS Lett. 579:2236-2242(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-832 AND VAL-834, INDUCTION.
  6. "Sema4C participates in myogenic differentiation in vivo and in vitro through the p38 MAPK pathway."
    Wu H., Wang X., Liu S., Wu Y., Zhao T., Chen X., Zhu L., Wu Y., Ding X., Peng X., Yuan J., Wang X., Fan W., Fan M.
    Eur. J. Cell Biol. 86:331-344(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  7. "Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo."
    Deng S., Hirschberg A., Worzfeld T., Penachioni J.Y., Korostylev A., Swiercz J.M., Vodrazka P., Mauti O., Stoeckli E.T., Tamagnone L., Offermanns S., Kuner R.
    J. Neurosci. 27:6333-6347(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PLXNB2.
  8. Cited for: FUNCTION, INTERACTION WITH PLXNB2, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSEM4C_MOUSE
AccessioniPrimary (citable) accession number: Q64151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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