Reviewed, UniProtKB/Swiss-Prot Q64148 (CP3AA_MESAU)

Names and origin

Protein names

Recommended name:
    Lithocholate 6-beta-hydroxylase
      Short name=6 beta-hydroxylase
    EC=1.14.13.94
Alternative name(s):
    Cytochrome P450 3A10
    CYPIIIA10

Gene names

Name:

CYP3A10

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 6 beta-hydroxylation of lithocholic acid and steroid hormones.
Catalytic activity	Lithocholate + NADPH + O2 = 6-beta-hydroxylithocholate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Developmental stage	Expressed only in male hamsters.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro lithocholate 6beta-hydroxylase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Lithocholate 6-beta-hydroxylase		PRO_0000051793
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64148-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: D4D24FEE87FD7F51	FASTA	503	57,693
	10 20 30 40 50 60 MELIPNLSIE TWVLLAISLV LIYIYGTYSH GTFKKLGIPG PKPLPFFGTI LGYHDGTWKF 70 80 90 100 110 120 DETCYKKYGK IWGFYDGRVP VLAIADPEII KTVLVKECYS NFTNRRSFGP VGFMKKSITI 130 140 150 160 170 180 SKDEEWKRLR TLLSPAFTSG KLKEMFPIIG QYGDTLVKNL RREEEKGKPV NMKEILGAYS 190 200 210 220 230 240 MDVITGTSFG VNVDSLNNPE DPFVQKARKI LKFNFFDPFI LSIILFPFLT TIYDLLRFSI 250 260 270 280 290 300 FPRQSTNFFK KFITTMKKNR LHSNQKTRMD FFQLMMNTQN SKGKESQKAL SDLEMAAQAI 310 320 330 340 350 360 IFIFAGYEST STSICLVLYE LATHPDVQKK LHDEIDSALP NKAPVTYDVL MGMEYLDMVI 370 380 390 400 410 420 NEGLRLYPIA NRLERISKKA VEINGLFIPK GITVMVPTYP LHRDPEYWPE PEEFRPERFS 430 440 450 460 470 480 KENKGSIDPY VYMPFGNGPR NCIGMRFALL SMKLAVVSVL QNFTLQTCEQ TENHLKFARQ 490 500 IILQPENPII LKIISRDKPI TGA

References

[1]	"Cloning, expression and regulation of lithocholic acid 6beta-hydroxylase." Teixeira J., Gil G. J. Biol. Chem. 266:21030-21036(1991) [PubMed: 1840595] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A STAT factor mediates the sexually dimorphic regulation of hepatic cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase gene expression by growth hormone." Subramanian A., Teixeira J., Wang J., Gil G. Mol. Cell. Biol. 15:4672-4682(1995) [PubMed: 7651384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[3]	"The lithocholic acid 6 beta-hydroxylase cytochrome P-450, CYP 3A10, is an active catalyst of steroid-hormone 6 beta-hydroxylation." Chang T.K.H., Teixeira J., Gil G., Waxman D.J. Biochem. J. 291:429-433(1993) [PubMed: 8484723] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73992 mRNA. No translation available. S79317 Genomic DNA. Translation: AAB35091.1.
PIR	A40843.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q64148.
Enzyme and pathway databases
BRENDA	1.14.13.94. 824.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP3AA_MESAU

Accession

Primary (citable) accession number: Q64148

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 15, 1999
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)