Reviewed, UniProtKB/Swiss-Prot Q64133 (AOFA_MOUSE)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] A
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type A
      Short name=MAO-A

Gene names

Name:

Maoa

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine By similarity.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Neurotransmitter degradation
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	dopamine catabolic process Inferred from direct assay. Source: MGI neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Amine oxidase [flavin-containing] A		PRO_0000099851
Regions
	Topological domain	1 – 497	497	Cytoplasmic By similarity
	Transmembrane	498 – 518	21	Anchor for type IV membrane protein By similarity
	Topological domain	519 – 526	8	Mitochondrial intermembrane By similarity
	Region	520 – 522	3	Interaction with membrane phospholipid headgroups By similarity
Sites
	Site	335	1	Important for substrate specificity By similarity
	Site	374	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	406	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64133-1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: 47FACA3581758217	FASTA	526	59,588
	10 20 30 40 50 60 MTDLEKPSIT GHMFDVVVIG GGISGLAAAK LLSEYKINVL VLEARDRVGG RTYTVRNEHV 70 80 90 100 110 120 KWVDVGGAYV GPTQNRILRL SKDLGIETYK VNVNERLVQY VKGKTYPFRG AFPPVWNPLA 130 140 150 160 170 180 YLDYNNLWRT MDDMGKEIPV DAPWQARHAE EWDKITMKDL IDKICWTKTA REFAYLFVNI 190 200 210 220 230 240 NVTSEPHEVS ALWFLWYVRQ CGGTSRIFSV TNGGQERKFV GGSGQISEQI MVLLGDKVKL 250 260 270 280 290 300 SSPVTYIDQT DDNIIIETLN HEHYECKYVI SAIPPVLTAK IHFKPELPPE RNQLIQRLPM 310 320 330 340 350 360 GAVIKCMVYY KEAFWKKKDY CGCMIIEDEE APISITLDDT KPDGSMPAIM GFILARKAER 370 380 390 400 410 420 LAKLHKDIRK RKICELYAKV LGSQEALSPV HYEEKNWCEE QYSGGCYTAY FPPGIMTLYG 430 440 450 460 470 480 RVIRQPVGRI YFAGTETATQ WSGYMEGAVE AGERAAREVL NALGKVAKKD IWVQEPESKD 490 500 510 520 VPALEITHTF LERNLPSVPG LLKITGFSTS VALLCFVLYK FKQPQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Aggressive behavior and altered amounts of brain serotonin and norepinephrine in mice lacking MAOA." Cases O., Seif I., Grimsby J., Gaspar P., Chen K., Pournin S., Mueller U., Aguet M., Babinet C., Shih J.C., de Maeyer E. Science 268:1763-1766(1995) [PubMed: 7792602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-40.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 137-147; 207-217; 268-280 AND 380-395, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC029100 mRNA. Translation: AAH29100.1. S78615, S78606 Genomic DNA. Translation: AAB34677.1.
IPI	IPI00169711.
UniGene	Mm.21108
3D structure databases
SMR	Q64133. Positions 23-509.
ModBase	Search...
Protein-protein interaction databases
STRING	Q64133.
PTM databases
PhosphoSite	Q64133.
Proteomic databases
PRIDE	Q64133.
Genome annotation databases
Ensembl	ENSMUST00000026013; ENSMUSP00000026013; ENSMUSG00000025037; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:96915. Maoa.
Phylogenomic databases
HOGENOM	Q64133.
HOVERGEN	Q64133.
Enzyme and pathway databases
BRENDA	1.4.3.4. 244.
Gene expression databases
ArrayExpress	Q64133.
Bgee	Q64133.
CleanEx	MM_MAOA.
Genevestigator	Q64133.
GermOnline	ENSMUSG00000025037. Mus musculus.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AOFA_MOUSE

Accession

Primary (citable) accession number: Q64133
Secondary accession number(s): Q8K0Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)