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Q64131

- RUNX3_MOUSE

UniProt

Q64131 - RUNX3_MOUSE

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Protein
Runt-related transcription factor 3
Gene
Runx3, Aml2, Cbfa3, Pebp2a3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation By similarity.

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. DNA binding Source: MGI
  3. RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  4. protein binding Source: MGI
  5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL

GO - Biological processi

  1. axon guidance Source: MGI
  2. cell maturation Source: MGI
  3. chondrocyte differentiation Source: MGI
  4. hair follicle morphogenesis Source: MGI
  5. interferon-gamma production Source: MGI
  6. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
  7. negative regulation of cell cycle Source: UniProtKB
  8. negative regulation of epithelial cell proliferation Source: UniProtKB
  9. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  10. neuron projection development Source: MGI
  11. positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  12. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 3
Alternative name(s):
Acute myeloid leukemia 2 protein
Core-binding factor subunit alpha-3
Short name:
CBF-alpha-3
Oncogene AML-2
Polyomavirus enhancer-binding protein 2 alpha C subunit
Short name:
PEA2-alpha C
Short name:
PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit
Gene namesi
Name:Runx3
Synonyms:Aml2, Cbfa3, Pebp2a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:102672. Runx3.

Subcellular locationi

Nucleus. Cytoplasm By similarity
Note: The tyrosine phosphorylated form localizes to the cytoplasm By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nuclear chromatin Source: BHF-UCL
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Runt-related transcription factor 3
PRO_0000174663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei241 – 2411Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ64131.

PTM databases

PhosphoSiteiQ64131.

Expressioni

Gene expression databases

ArrayExpressiQ64131.
BgeeiQ64131.
CleanExiMM_RUNX3.
GenevestigatoriQ64131.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK By similarity.

Protein-protein interaction databases

DIPiDIP-60276N.
IntActiQ64131. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64131.
SMRiQ64131. Positions 58-203.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 183129Runt
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 286Poly-Gly

Domaini

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Sequence similaritiesi

Contains 1 Runt domain.

Phylogenomic databases

eggNOGiNOG123889.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51062. RUNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64131-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR    50
PEVRSMVDVL ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG 100
DVPDGTVVTV MAGNDENYSA ELRNASAVMK NQVARFNDLR FVGRSGRGKS 150
FTLTITVFTN PTQVATYHRA IKVTVDGPRE PRRHRQKIED QTKAFPDRFG 200
DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF SDPRQFDRSF 250
PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR 300
FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE 350
RSPTRMLTSC PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR 400
MDEAVWRPY 409
Length:409
Mass (Da):43,518
Last modified:July 27, 2011 - v3
Checksum:iD2BCEBAF808A315D
GO
Isoform 2 (identifier: Q64131-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASNSIFDSFPQLYTNLHT

Show »
Length:423
Mass (Da):45,089
Checksum:i61F218AB7DC04D52
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2.
VSP_005950

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti227 – 2282AQ → PK in AAD38985. 1 Publication
Sequence conflicti234 – 2363SSD → FLN in AAD38985. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155880 mRNA. Translation: AAD38985.1.
AL731718 Genomic DNA. Translation: CAQ51606.1.
CU459015 Genomic DNA. Translation: CAQ52301.1.
CU459014 Genomic DNA. Translation: CAQ52315.1.
CH466552 Genomic DNA. Translation: EDL29993.1.
S78518 Genomic DNA. Translation: AAB34843.1.
AF169246 Genomic DNA. Translation: AAD46381.1.
AF321443 Genomic DNA. Translation: AAK11181.1.
CCDSiCCDS18782.1. [Q64131-2]
PIRiA56842.
A56843.
UniGeneiMm.378894.

Genome annotation databases

EnsembliENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
UCSCiuc008vgc.1. mouse. [Q64131-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155880 mRNA. Translation: AAD38985.1 .
AL731718 Genomic DNA. Translation: CAQ51606.1 .
CU459015 Genomic DNA. Translation: CAQ52301.1 .
CU459014 Genomic DNA. Translation: CAQ52315.1 .
CH466552 Genomic DNA. Translation: EDL29993.1 .
S78518 Genomic DNA. Translation: AAB34843.1 .
AF169246 Genomic DNA. Translation: AAD46381.1 .
AF321443 Genomic DNA. Translation: AAK11181.1 .
CCDSi CCDS18782.1. [Q64131-2 ]
PIRi A56842.
A56843.
UniGenei Mm.378894.

3D structure databases

ProteinModelPortali Q64131.
SMRi Q64131. Positions 58-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60276N.
IntActi Q64131. 1 interaction.

PTM databases

PhosphoSitei Q64131.

Proteomic databases

PRIDEi Q64131.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000119564 ; ENSMUSP00000113159 ; ENSMUSG00000070691 . [Q64131-1 ]
UCSCi uc008vgc.1. mouse. [Q64131-1 ]

Organism-specific databases

MGIi MGI:102672. Runx3.

Phylogenomic databases

eggNOGi NOG123889.
GeneTreei ENSGT00390000016964.
HOGENOMi HOG000045616.
HOVERGENi HBG060268.

Miscellaneous databases

PROi Q64131.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q64131.
Bgeei Q64131.
CleanExi MM_RUNX3.
Genevestigatori Q64131.

Family and domain databases

Gene3Di 2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProi IPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view ]
PANTHERi PTHR11950. PTHR11950. 1 hit.
Pfami PF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view ]
PIRSFi PIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSi PR00967. ONCOGENEAML1.
SUPFAMi SSF49417. SSF49417. 1 hit.
PROSITEi PS51062. RUNT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3."
    Yarmus M., Woolf E., Bernstein Y., Fainaru O., Negreanu V., Levanon D., Groner Y.
    Proc. Natl. Acad. Sci. U.S.A. 103:7384-7389(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter."
    Wijmenga C., Speck N.A., Dracopoli N.C., Hofker M.H., Liu P., Collins F.S.
    Genomics 26:611-614(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-167.
  5. "Identification and chromosomal mapping of a third mouse runt-like locus."
    Calabi F., Rhodes M., Williamson P., Boyd Y.
    Genomics 26:607-610(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
  6. "The mouse AML2 gene - proximal promoter region."
    Negreanu V., Levanon D., Bettoun J.D., Groner Y.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
    Strain: 129/Sv.
  7. Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).

Entry informationi

Entry nameiRUNX3_MOUSE
AccessioniPrimary (citable) accession number: Q64131
Secondary accession number(s): B2FDG4, Q99P92, Q9R199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi