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Q64131 (RUNX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Runt-related transcription factor 3
Alternative name(s):
Acute myeloid leukemia 2 protein
Core-binding factor subunit alpha-3
Short name=CBF-alpha-3
Oncogene AML-2
Polyomavirus enhancer-binding protein 2 alpha C subunit
Short name=PEA2-alpha C
Short name=PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit
Gene names
Name:Runx3
Synonyms:Aml2, Cbfa3, Pebp2a3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation By similarity.

Subunit structure

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK By similarity.

Subcellular location

Nucleus. Cytoplasm By similarity. Note: The tyrosine phosphorylated form localizes to the cytoplasm By similarity.

Domain

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Post-translational modification

Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN By similarity.

Sequence similarities

Contains 1 Runt domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from mutant phenotype PubMed 12352981. Source: MGI

cell maturation

Inferred from genetic interaction PubMed 15107406. Source: MGI

chondrocyte differentiation

Inferred from genetic interaction PubMed 22916278. Source: MGI

hair follicle morphogenesis

Inferred from mutant phenotype PubMed 15937937. Source: MGI

interferon-gamma production

Inferred from direct assay PubMed 20399120. Source: MGI

mRNA transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17352693. Source: BHF-UCL

negative regulation of cell cycle

Inferred from mutant phenotype PubMed 11955451. Source: UniProtKB

negative regulation of epithelial cell proliferation

Inferred from mutant phenotype PubMed 11955451. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17352693. Source: BHF-UCL

neuron projection development

Inferred from mutant phenotype PubMed 12352981. Source: MGI

positive regulation of extrinsic apoptotic signaling pathway

Inferred from mutant phenotype PubMed 11955451. Source: MGI

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nuclear chromatin

Inferred from direct assay PubMed 17352693. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 15514019. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from direct assay PubMed 12807883. Source: MGI

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 17352693. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 20399120. Source: MGI

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay PubMed 17352693. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q64131-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q64131-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASNSIFDSFPQLYTNLHT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Runt-related transcription factor 3
PRO_0000174663

Regions

Domain55 – 183129Runt
Compositional bias23 – 286Poly-Gly

Amino acid modifications

Modified residue2411Phosphoserine By similarity

Natural variations

Alternative sequence1 – 55MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2.
VSP_005950

Experimental info

Sequence conflict227 – 2282AQ → PK in AAD38985. Ref.1
Sequence conflict234 – 2363SSD → FLN in AAD38985. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: D2BCEBAF808A315D

FASTA40943,518
        10         20         30         40         50         60 
MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR PEVRSMVDVL 

        70         80         90        100        110        120 
ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG DVPDGTVVTV MAGNDENYSA 

       130        140        150        160        170        180 
ELRNASAVMK NQVARFNDLR FVGRSGRGKS FTLTITVFTN PTQVATYHRA IKVTVDGPRE 

       190        200        210        220        230        240 
PRRHRQKIED QTKAFPDRFG DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF 

       250        260        270        280        290        300 
SDPRQFDRSF PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR 

       310        320        330        340        350        360 
FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE RSPTRMLTSC 

       370        380        390        400 
PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR MDEAVWRPY 

« Hide

Isoform 2 [UniParc].

Checksum: 61F218AB7DC04D52
Show »

FASTA42345,089

References

« Hide 'large scale' references
[1]"Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3."
Yarmus M., Woolf E., Bernstein Y., Fainaru O., Negreanu V., Levanon D., Groner Y.
Proc. Natl. Acad. Sci. U.S.A. 103:7384-7389(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Spleen.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter."
Wijmenga C., Speck N.A., Dracopoli N.C., Hofker M.H., Liu P., Collins F.S.
Genomics 26:611-614(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-167.
[5]"Identification and chromosomal mapping of a third mouse runt-like locus."
Calabi F., Rhodes M., Williamson P., Boyd Y.
Genomics 26:607-610(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
[6]"The mouse AML2 gene - proximal promoter region."
Negreanu V., Levanon D., Bettoun J.D., Groner Y.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
Strain: 129/Sv.
[7]"The RUNX3 gene -- sequence, structure and regulated expression."
Bangsow C., Rubins N., Glusman G., Bernstein Y., Negreanu V., Goldenberg D., Lotem J., Ben-Asher E., Lancet D., Levanon D., Groner Y.
Gene 279:221-232(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF155880 mRNA. Translation: AAD38985.1.
AL731718 Genomic DNA. Translation: CAQ51606.1.
CU459015 Genomic DNA. Translation: CAQ52301.1.
CU459014 Genomic DNA. Translation: CAQ52315.1.
CH466552 Genomic DNA. Translation: EDL29993.1.
S78518 Genomic DNA. Translation: AAB34843.1.
AF169246 Genomic DNA. Translation: AAD46381.1.
AF321443 Genomic DNA. Translation: AAK11181.1.
CCDSCCDS18782.1. [Q64131-2]
PIRA56842.
A56843.
UniGeneMm.378894.

3D structure databases

ProteinModelPortalQ64131.
SMRQ64131. Positions 58-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60276N.
IntActQ64131. 1 interaction.

PTM databases

PhosphoSiteQ64131.

Proteomic databases

PRIDEQ64131.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
UCSCuc008vgc.1. mouse. [Q64131-1]

Organism-specific databases

MGIMGI:102672. Runx3.

Phylogenomic databases

eggNOGNOG123889.
GeneTreeENSGT00390000016964.
HOGENOMHOG000045616.
HOVERGENHBG060268.

Gene expression databases

ArrayExpressQ64131.
BgeeQ64131.
CleanExMM_RUNX3.
GenevestigatorQ64131.

Family and domain databases

Gene3D2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERPTHR11950. PTHR11950. 1 hit.
PfamPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSPR00967. ONCOGENEAML1.
SUPFAMSSF49417. SSF49417. 1 hit.
PROSITEPS51062. RUNT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ64131.
SOURCESearch...

Entry information

Entry nameRUNX3_MOUSE
AccessionPrimary (citable) accession number: Q64131
Secondary accession number(s): B2FDG4, Q99P92, Q9R199
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot