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Protein

Runt-related transcription factor 3

Gene

Runx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. DNA binding Source: MGI
  3. RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL

GO - Biological processi

  1. axon guidance Source: MGI
  2. cell maturation Source: MGI
  3. chondrocyte differentiation Source: MGI
  4. hair follicle morphogenesis Source: MGI
  5. interferon-gamma production Source: MGI
  6. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
  7. negative regulation of cell cycle Source: UniProtKB
  8. negative regulation of epithelial cell proliferation Source: UniProtKB
  9. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  10. neuron projection development Source: MGI
  11. positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  12. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 3
Alternative name(s):
Acute myeloid leukemia 2 protein
Core-binding factor subunit alpha-3
Short name:
CBF-alpha-3
Oncogene AML-2
Polyomavirus enhancer-binding protein 2 alpha C subunit
Short name:
PEA2-alpha C
Short name:
PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit
Gene namesi
Name:Runx3
Synonyms:Aml2, Cbfa3, Pebp2a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:102672. Runx3.

Subcellular locationi

Nucleus. Cytoplasm By similarity
Note: The tyrosine phosphorylated form localizes to the cytoplasm.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nuclear chromatin Source: BHF-UCL
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Runt-related transcription factor 3PRO_0000174663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei241 – 2411PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ64131.

PTM databases

PhosphoSiteiQ64131.

Expressioni

Gene expression databases

BgeeiQ64131.
CleanExiMM_RUNX3.
ExpressionAtlasiQ64131. baseline and differential.
GenevestigatoriQ64131.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60276N.
IntActiQ64131. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64131.
SMRiQ64131. Positions 65-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 183129RuntPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 286Poly-Gly

Domaini

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Sequence similaritiesi

Contains 1 Runt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG123889.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.
InParanoidiQ64131.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51062. RUNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64131-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR
60 70 80 90 100
PEVRSMVDVL ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG
110 120 130 140 150
DVPDGTVVTV MAGNDENYSA ELRNASAVMK NQVARFNDLR FVGRSGRGKS
160 170 180 190 200
FTLTITVFTN PTQVATYHRA IKVTVDGPRE PRRHRQKIED QTKAFPDRFG
210 220 230 240 250
DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF SDPRQFDRSF
260 270 280 290 300
PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR
310 320 330 340 350
FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE
360 370 380 390 400
RSPTRMLTSC PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR

MDEAVWRPY
Length:409
Mass (Da):43,518
Last modified:July 27, 2011 - v3
Checksum:iD2BCEBAF808A315D
GO
Isoform 2 (identifier: Q64131-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASNSIFDSFPQLYTNLHT

Show »
Length:423
Mass (Da):45,089
Checksum:i61F218AB7DC04D52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti227 – 2282AQ → PK in AAD38985. (PubMed:16651517)Curated
Sequence conflicti234 – 2363SSD → FLN in AAD38985. (PubMed:16651517)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2. CuratedVSP_005950

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155880 mRNA. Translation: AAD38985.1.
AL731718 Genomic DNA. Translation: CAQ51606.1.
CU459015 Genomic DNA. Translation: CAQ52301.1.
CU459014 Genomic DNA. Translation: CAQ52315.1.
CH466552 Genomic DNA. Translation: EDL29993.1.
S78518 Genomic DNA. Translation: AAB34843.1.
AF169246 Genomic DNA. Translation: AAD46381.1.
AF321443 Genomic DNA. Translation: AAK11181.1.
CCDSiCCDS18782.1. [Q64131-2]
PIRiA56842.
A56843.
UniGeneiMm.378894.

Genome annotation databases

EnsembliENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
UCSCiuc008vgc.1. mouse. [Q64131-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155880 mRNA. Translation: AAD38985.1.
AL731718 Genomic DNA. Translation: CAQ51606.1.
CU459015 Genomic DNA. Translation: CAQ52301.1.
CU459014 Genomic DNA. Translation: CAQ52315.1.
CH466552 Genomic DNA. Translation: EDL29993.1.
S78518 Genomic DNA. Translation: AAB34843.1.
AF169246 Genomic DNA. Translation: AAD46381.1.
AF321443 Genomic DNA. Translation: AAK11181.1.
CCDSiCCDS18782.1. [Q64131-2]
PIRiA56842.
A56843.
UniGeneiMm.378894.

3D structure databases

ProteinModelPortaliQ64131.
SMRiQ64131. Positions 65-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60276N.
IntActiQ64131. 1 interaction.

PTM databases

PhosphoSiteiQ64131.

Proteomic databases

PRIDEiQ64131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
UCSCiuc008vgc.1. mouse. [Q64131-1]

Organism-specific databases

MGIiMGI:102672. Runx3.

Phylogenomic databases

eggNOGiNOG123889.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.
InParanoidiQ64131.

Miscellaneous databases

PROiQ64131.
SOURCEiSearch...

Gene expression databases

BgeeiQ64131.
CleanExiMM_RUNX3.
ExpressionAtlasiQ64131. baseline and differential.
GenevestigatoriQ64131.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51062. RUNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3."
    Yarmus M., Woolf E., Bernstein Y., Fainaru O., Negreanu V., Levanon D., Groner Y.
    Proc. Natl. Acad. Sci. U.S.A. 103:7384-7389(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter."
    Wijmenga C., Speck N.A., Dracopoli N.C., Hofker M.H., Liu P., Collins F.S.
    Genomics 26:611-614(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-167.
  5. "Identification and chromosomal mapping of a third mouse runt-like locus."
    Calabi F., Rhodes M., Williamson P., Boyd Y.
    Genomics 26:607-610(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
  6. "The mouse AML2 gene - proximal promoter region."
    Negreanu V., Levanon D., Bettoun J.D., Groner Y.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
    Strain: 129/Sv.
  7. Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).

Entry informationi

Entry nameiRUNX3_MOUSE
AccessioniPrimary (citable) accession number: Q64131
Secondary accession number(s): B2FDG4, Q99P92, Q9R199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.