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Protein

Runt-related transcription factor 3

Gene

Runx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: InterPro
  • DNA binding Source: MGI
  • histone deacetylase binding Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • SMAD binding Source: MGI

GO - Biological processi

  • axon guidance Source: MGI
  • cell maturation Source: MGI
  • chondrocyte differentiation Source: MGI
  • hair follicle morphogenesis Source: MGI
  • hemopoiesis Source: GO_Central
  • interferon-gamma production Source: MGI
  • mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of epithelial cell proliferation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • neuron projection development Source: MGI
  • ossification Source: GO_Central
  • positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein phosphorylation Source: UniProtKB
  • regulation of cell differentiation Source: GO_Central
  • response to transforming growth factor beta Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-8941855. RUNX3 regulates CDKN1A transcription.
R-MMU-8941856. RUNX3 regulates NOTCH signaling.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-8949275. RUNX3 Regulates Immune Response and Cell Migration.
R-MMU-8951430. RUNX3 regulates WNT signaling.
R-MMU-8951671. RUNX3 regulates YAP1-mediated transcription.
R-MMU-8951936. RUNX3 regulates p14-ARF.

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 3
Alternative name(s):
Acute myeloid leukemia 2 protein
Core-binding factor subunit alpha-3
Short name:
CBF-alpha-3
Oncogene AML-2
Polyomavirus enhancer-binding protein 2 alpha C subunit
Short name:
PEA2-alpha C
Short name:
PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit
Gene namesi
Name:Runx3
Synonyms:Aml2, Cbfa3, Pebp2a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:102672. Runx3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746631 – 409Runt-related transcription factor 3Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki193Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei241PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ64131.
PaxDbiQ64131.
PRIDEiQ64131.

PTM databases

iPTMnetiQ64131.
PhosphoSitePlusiQ64131.

Expressioni

Gene expression databases

BgeeiENSMUSG00000070691.
CleanExiMM_RUNX3.
ExpressionAtlasiQ64131. baseline and differential.
GenevisibleiQ64131. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Interacts with FOXP3. Interacts with ZFHX3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60276N.
ELMiQ64131.
IntActiQ64131. 1 interactor.
STRINGi10090.ENSMUSP00000050353.

Structurei

3D structure databases

ProteinModelPortaliQ64131.
SMRiQ64131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 183RuntPROSITE-ProRule annotationAdd BLAST129

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 28Poly-Gly6

Domaini

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Phylogenomic databases

eggNOGiKOG3982. Eukaryota.
ENOG4111J4Y. LUCA.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.
InParanoidiQ64131.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiView protein in InterPro
IPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiView protein in Pfam
PF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiView protein in PROSITE
PS51062. RUNT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64131-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR
60 70 80 90 100
PEVRSMVDVL ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG
110 120 130 140 150
DVPDGTVVTV MAGNDENYSA ELRNASAVMK NQVARFNDLR FVGRSGRGKS
160 170 180 190 200
FTLTITVFTN PTQVATYHRA IKVTVDGPRE PRRHRQKIED QTKAFPDRFG
210 220 230 240 250
DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF SDPRQFDRSF
260 270 280 290 300
PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR
310 320 330 340 350
FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE
360 370 380 390 400
RSPTRMLTSC PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR

MDEAVWRPY
Length:409
Mass (Da):43,518
Last modified:July 27, 2011 - v3
Checksum:iD2BCEBAF808A315D
GO
Isoform 2 (identifier: Q64131-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASNSIFDSFPQLYTNLHT

Show »
Length:423
Mass (Da):45,089
Checksum:i61F218AB7DC04D52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti227 – 228AQ → PK in AAD38985 (PubMed:16651517).Curated2
Sequence conflicti234 – 236SSD → FLN in AAD38985 (PubMed:16651517).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0059501 – 5MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2. Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155880 mRNA. Translation: AAD38985.1.
AL731718 Genomic DNA. Translation: CAQ51606.1.
CU459015 Genomic DNA. Translation: CAQ52301.1.
CU459014 Genomic DNA. Translation: CAQ52315.1.
CH466552 Genomic DNA. Translation: EDL29993.1.
S78518 Genomic DNA. Translation: AAB34843.1.
AF169246 Genomic DNA. Translation: AAD46381.1.
AF321443 Genomic DNA. Translation: AAK11181.1.
CCDSiCCDS18782.1. [Q64131-2]
PIRiA56842.
A56843.
UniGeneiMm.378894.

Genome annotation databases

EnsembliENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
UCSCiuc008vgc.1. mouse. [Q64131-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRUNX3_MOUSE
AccessioniPrimary (citable) accession number: Q64131
Secondary accession number(s): B2FDG4, Q99P92, Q9R199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot