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Q64131

- RUNX3_MOUSE

UniProt

Q64131 - RUNX3_MOUSE

Protein

Runt-related transcription factor 3

Gene

Runx3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. DNA binding Source: MGI
    3. protein binding Source: MGI
    4. RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
    5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL

    GO - Biological processi

    1. axon guidance Source: MGI
    2. cell maturation Source: MGI
    3. chondrocyte differentiation Source: MGI
    4. hair follicle morphogenesis Source: MGI
    5. interferon-gamma production Source: MGI
    6. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
    7. negative regulation of cell cycle Source: UniProtKB
    8. negative regulation of epithelial cell proliferation Source: UniProtKB
    9. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    10. neuron projection development Source: MGI
    11. positive regulation of extrinsic apoptotic signaling pathway Source: MGI
    12. protein phosphorylation Source: UniProtKB

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Runt-related transcription factor 3
    Alternative name(s):
    Acute myeloid leukemia 2 protein
    Core-binding factor subunit alpha-3
    Short name:
    CBF-alpha-3
    Oncogene AML-2
    Polyomavirus enhancer-binding protein 2 alpha C subunit
    Short name:
    PEA2-alpha C
    Short name:
    PEBP2-alpha C
    SL3-3 enhancer factor 1 alpha C subunit
    SL3/AKV core-binding factor alpha C subunit
    Gene namesi
    Name:Runx3
    Synonyms:Aml2, Cbfa3, Pebp2a3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:102672. Runx3.

    Subcellular locationi

    Nucleus. Cytoplasm By similarity
    Note: The tyrosine phosphorylated form localizes to the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nuclear chromatin Source: BHF-UCL
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 409409Runt-related transcription factor 3PRO_0000174663Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei241 – 2411PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ64131.

    PTM databases

    PhosphoSiteiQ64131.

    Expressioni

    Gene expression databases

    ArrayExpressiQ64131.
    BgeeiQ64131.
    CleanExiMM_RUNX3.
    GenevestigatoriQ64131.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-60276N.
    IntActiQ64131. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64131.
    SMRiQ64131. Positions 58-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 183129RuntPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi23 – 286Poly-Gly

    Domaini

    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

    Sequence similaritiesi

    Contains 1 Runt domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG123889.
    GeneTreeiENSGT00390000016964.
    HOGENOMiHOG000045616.
    HOVERGENiHBG060268.

    Family and domain databases

    Gene3Di2.60.40.720. 1 hit.
    4.10.770.10. 1 hit.
    InterProiIPR000040. AML1_Runt.
    IPR008967. p53-like_TF_DNA-bd.
    IPR012346. p53/RUNT-type_TF_DNA-bd.
    IPR013524. Runt_dom.
    IPR027384. Runx_central_dom.
    IPR013711. RunxI_C_dom.
    IPR016554. TF_Runt-rel_RUNX.
    [Graphical view]
    PANTHERiPTHR11950. PTHR11950. 1 hit.
    PfamiPF00853. Runt. 1 hit.
    PF08504. RunxI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
    PRINTSiPR00967. ONCOGENEAML1.
    SUPFAMiSSF49417. SSF49417. 1 hit.
    PROSITEiPS51062. RUNT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64131-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR    50
    PEVRSMVDVL ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG 100
    DVPDGTVVTV MAGNDENYSA ELRNASAVMK NQVARFNDLR FVGRSGRGKS 150
    FTLTITVFTN PTQVATYHRA IKVTVDGPRE PRRHRQKIED QTKAFPDRFG 200
    DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF SDPRQFDRSF 250
    PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR 300
    FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE 350
    RSPTRMLTSC PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR 400
    MDEAVWRPY 409
    Length:409
    Mass (Da):43,518
    Last modified:July 27, 2011 - v3
    Checksum:iD2BCEBAF808A315D
    GO
    Isoform 2 (identifier: Q64131-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-5: MRIPV → MASNSIFDSFPQLYTNLHT

    Show »
    Length:423
    Mass (Da):45,089
    Checksum:i61F218AB7DC04D52
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti227 – 2282AQ → PK in AAD38985. (PubMed:16651517)Curated
    Sequence conflicti234 – 2363SSD → FLN in AAD38985. (PubMed:16651517)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 55MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2. CuratedVSP_005950

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155880 mRNA. Translation: AAD38985.1.
    AL731718 Genomic DNA. Translation: CAQ51606.1.
    CU459015 Genomic DNA. Translation: CAQ52301.1.
    CU459014 Genomic DNA. Translation: CAQ52315.1.
    CH466552 Genomic DNA. Translation: EDL29993.1.
    S78518 Genomic DNA. Translation: AAB34843.1.
    AF169246 Genomic DNA. Translation: AAD46381.1.
    AF321443 Genomic DNA. Translation: AAK11181.1.
    CCDSiCCDS18782.1. [Q64131-2]
    PIRiA56842.
    A56843.
    UniGeneiMm.378894.

    Genome annotation databases

    EnsembliENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691. [Q64131-1]
    UCSCiuc008vgc.1. mouse. [Q64131-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155880 mRNA. Translation: AAD38985.1 .
    AL731718 Genomic DNA. Translation: CAQ51606.1 .
    CU459015 Genomic DNA. Translation: CAQ52301.1 .
    CU459014 Genomic DNA. Translation: CAQ52315.1 .
    CH466552 Genomic DNA. Translation: EDL29993.1 .
    S78518 Genomic DNA. Translation: AAB34843.1 .
    AF169246 Genomic DNA. Translation: AAD46381.1 .
    AF321443 Genomic DNA. Translation: AAK11181.1 .
    CCDSi CCDS18782.1. [Q64131-2 ]
    PIRi A56842.
    A56843.
    UniGenei Mm.378894.

    3D structure databases

    ProteinModelPortali Q64131.
    SMRi Q64131. Positions 58-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60276N.
    IntActi Q64131. 1 interaction.

    PTM databases

    PhosphoSitei Q64131.

    Proteomic databases

    PRIDEi Q64131.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000119564 ; ENSMUSP00000113159 ; ENSMUSG00000070691 . [Q64131-1 ]
    UCSCi uc008vgc.1. mouse. [Q64131-1 ]

    Organism-specific databases

    MGIi MGI:102672. Runx3.

    Phylogenomic databases

    eggNOGi NOG123889.
    GeneTreei ENSGT00390000016964.
    HOGENOMi HOG000045616.
    HOVERGENi HBG060268.

    Miscellaneous databases

    PROi Q64131.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q64131.
    Bgeei Q64131.
    CleanExi MM_RUNX3.
    Genevestigatori Q64131.

    Family and domain databases

    Gene3Di 2.60.40.720. 1 hit.
    4.10.770.10. 1 hit.
    InterProi IPR000040. AML1_Runt.
    IPR008967. p53-like_TF_DNA-bd.
    IPR012346. p53/RUNT-type_TF_DNA-bd.
    IPR013524. Runt_dom.
    IPR027384. Runx_central_dom.
    IPR013711. RunxI_C_dom.
    IPR016554. TF_Runt-rel_RUNX.
    [Graphical view ]
    PANTHERi PTHR11950. PTHR11950. 1 hit.
    Pfami PF00853. Runt. 1 hit.
    PF08504. RunxI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009374. TF_Runt-rel_RUNX. 1 hit.
    PRINTSi PR00967. ONCOGENEAML1.
    SUPFAMi SSF49417. SSF49417. 1 hit.
    PROSITEi PS51062. RUNT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3."
      Yarmus M., Woolf E., Bernstein Y., Fainaru O., Negreanu V., Levanon D., Groner Y.
      Proc. Natl. Acad. Sci. U.S.A. 103:7384-7389(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter."
      Wijmenga C., Speck N.A., Dracopoli N.C., Hofker M.H., Liu P., Collins F.S.
      Genomics 26:611-614(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-167.
    5. "Identification and chromosomal mapping of a third mouse runt-like locus."
      Calabi F., Rhodes M., Williamson P., Boyd Y.
      Genomics 26:607-610(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
    6. "The mouse AML2 gene - proximal promoter region."
      Negreanu V., Levanon D., Bettoun J.D., Groner Y.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
      Strain: 129/Sv.
    7. Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).

    Entry informationi

    Entry nameiRUNX3_MOUSE
    AccessioniPrimary (citable) accession number: Q64131
    Secondary accession number(s): B2FDG4, Q99P92, Q9R199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3