Q64131 (RUNX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Runt-related transcription factor 3

Alternative name(s):
Acute myeloid leukemia 2 protein
Core-binding factor subunit alpha-3
Short name=CBF-alpha-3
Oncogene AML-2
Polyomavirus enhancer-binding protein 2 alpha C subunit
Short name=PEA2-alpha C
Short name=PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit

Gene names

Name:	Runx3
Synonyms:	Aml2, Cbfa3, Pebp2a3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity. Note: The tyrosine phosphorylated form localizes to the cytoplasm By similarity.
Domain	A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational modification	Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN By similarity.
Sequence similarities	Contains 1 Runt domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from mutant phenotype PubMed 12352981. Source: MGI cell maturation Inferred from genetic interaction PubMed 15107406. Source: MGI chondrocyte differentiation Inferred from genetic interaction PubMed 22916278. Source: MGI hair follicle morphogenesis Inferred from mutant phenotype PubMed 15937937. Source: MGI induction of apoptosis Inferred from mutant phenotype PubMed 11955451. Source: UniProtKB interferon-gamma production Inferred from direct assay PubMed 20399120. Source: MGI mRNA transcription from RNA polymerase II promoter Inferred from direct assay PubMed 17352693. Source: BHF-UCL negative regulation of cell cycle Inferred from mutant phenotype PubMed 11955451. Source: UniProtKB negative regulation of epithelial cell proliferation Inferred from mutant phenotype PubMed 11955451. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 17352693. Source: BHF-UCL protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nuclear chromatin Inferred from direct assay PubMed 17352693. Source: BHF-UCL
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro RNA polymerase II regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 17352693. Source: BHF-UCL sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay PubMed 17352693. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q64131-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q64131-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRIPV → MASNSIFDSFPQLYTNLHT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Runt-related transcription factor 3		PRO_0000174663
Regions
	Domain	55 – 183	129	Runt
	Compositional bias	23 – 28	6	Poly-Gly
Amino acid modifications
	Modified residue	241	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 5	5	MRIPV → MASNSIFDSFPQLYTNLHT in isoform 2.		VSP_005950
Experimental info
	Sequence conflict	227 – 228	2	AQ → PK in AAD38985. Ref.1
	Sequence conflict	234 – 236	3	SSD → FLN in AAD38985. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: D2BCEBAF808A315D	FASTA	409	43,518
	10 20 30 40 50 60 MRIPVDPSTS RRFTPPSTAF PCGGGGGGKM GENSGALSAQ ATAGPGGRTR PEVRSMVDVL 70 80 90 100 110 120 ADHAGELVRT DSPNFLCSVL PSHWRCNKTL PVAFKVVALG DVPDGTVVTV MAGNDENYSA 130 140 150 160 170 180 ELRNASAVMK NQVARFNDLR FVGRSGRGKS FTLTITVFTN PTQVATYHRA IKVTVDGPRE 190 200 210 220 230 240 PRRHRQKIED QTKAFPDRFG DLRMRVTPST PSPRGSLSTT SHFSSQAQTP IQGSSDLNPF 250 260 270 280 290 300 SDPRQFDRSF PTLQSLTESR FPDPRMHYPG AMSAAFPYSA TPSGTSLGSL SVAGMPASSR 310 320 330 340 350 360 FHHTYLPPPY PGAPQSQSGP FQANPAPYHL FYGASSGSYQ FSMAAAGGGE RSPTRMLTSC 370 380 390 400 PSGASVSAGN LMNPSLGQAD GVEADGSHSN SPTALSTPGR MDEAVWRPY
	Isoform 2 [UniParc]. Checksum: 61F218AB7DC04D52	FASTA	423	45,089
	10 20 30 40 50 60 MASNSIFDSF PQLYTNLHTD PSTSRRFTPP STAFPCGGGG GGKMGENSGA LSAQATAGPG 70 80 90 100 110 120 GRTRPEVRSM VDVLADHAGE LVRTDSPNFL CSVLPSHWRC NKTLPVAFKV VALGDVPDGT 130 140 150 160 170 180 VVTVMAGNDE NYSAELRNAS AVMKNQVARF NDLRFVGRSG RGKSFTLTIT VFTNPTQVAT 190 200 210 220 230 240 YHRAIKVTVD GPREPRRHRQ KIEDQTKAFP DRFGDLRMRV TPSTPSPRGS LSTTSHFSSQ 250 260 270 280 290 300 AQTPIQGSSD LNPFSDPRQF DRSFPTLQSL TESRFPDPRM HYPGAMSAAF PYSATPSGTS 310 320 330 340 350 360 LGSLSVAGMP ASSRFHHTYL PPPYPGAPQS QSGPFQANPA PYHLFYGASS GSYQFSMAAA 370 380 390 400 410 420 GGGERSPTRM LTSCPSGASV SAGNLMNPSL GQADGVEADG SHSNSPTALS TPGRMDEAVW RPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3." Yarmus M., Woolf E., Bernstein Y., Fainaru O., Negreanu V., Levanon D., Groner Y. Proc. Natl. Acad. Sci. U.S.A. 103:7384-7389(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Spleen.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter." Wijmenga C., Speck N.A., Dracopoli N.C., Hofker M.H., Liu P., Collins F.S. Genomics 26:611-614(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-167.
[5]	"Identification and chromosomal mapping of a third mouse runt-like locus." Calabi F., Rhodes M., Williamson P., Boyd Y. Genomics 26:607-610(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1).
[6]	"The mouse AML2 gene - proximal promoter region." Negreanu V., Levanon D., Bettoun J.D., Groner Y. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95 (ISOFORM 1). Strain: 129/Sv.
[7]	"The RUNX3 gene -- sequence, structure and regulated expression." Bangsow C., Rubins N., Glusman G., Bernstein Y., Negreanu V., Goldenberg D., Lotem J., Ben-Asher E., Lancet D., Levanon D., Groner Y. Gene 279:221-232(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155880 mRNA. Translation: AAD38985.1. AL731718 Genomic DNA. Translation: CAQ51606.1. CU459015 Genomic DNA. Translation: CAQ52301.1. CU459014 Genomic DNA. Translation: CAQ52315.1. CH466552 Genomic DNA. Translation: EDL29993.1. S78518 Genomic DNA. Translation: AAB34843.1. AF169246 Genomic DNA. Translation: AAD46381.1. AF321443 Genomic DNA. Translation: AAK11181.1.
IPI	IPI00230505. IPI00894855.
PIR	A56842. A56843.
UniGene	Mm.378894.
3D structure databases
ProteinModelPortal	Q64131.
SMR	Q64131. Positions 65-184.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q64131. 1 interaction.
PTM databases
PhosphoSite	Q64131.
Proteomic databases
PRIDE	Q64131.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000119564; ENSMUSP00000113159; ENSMUSG00000070691.
Organism-specific databases
MGI	MGI:102672. Runx3.
Phylogenomic databases
eggNOG	NOG123889.
GeneTree	ENSGT00390000016964.
HOGENOM	HOG000045616.
HOVERGEN	HBG060268.
OrthoDB	EOG47M1XZ.
Gene expression databases
ArrayExpress	Q64131.
Bgee	Q64131.
CleanEx	MM_RUNX3.
Genevestigator	Q64131.
GermOnline	ENSMUSG00000070691. Mus musculus.
Family and domain databases
Gene3D	2.60.40.720. 1 hit.
InterPro	IPR000040. AML1_Runt. IPR008967. p53-like_TF_DNA-bd. IPR012346. p53/RUNT-type_TF_DNA-bd. IPR013524. Runt_dom. IPR013711. RunxI_c_dom. IPR016554. TF_Runt-rel_RUNX. [Graphical view]
PANTHER	PTHR11950. PTHR11950. 1 hit.
Pfam	PF00853. Runt. 1 hit. PF08504. RunxI. 1 hit. [Graphical view]
PIRSF	PIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTS	PR00967. ONCOGENEAML1.
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS51062. RUNT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

RUNX3_MOUSE

Accession

Primary (citable) accession number: Q64131
Secondary accession number(s): B2FDG4, Q99P92, Q9R199

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program