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Protein

Basic helix-loop-helix transcription factor scleraxis

Gene

Scx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • DNA binding Source: MGI
  • E-box binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB
  • transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding Source: NTNU_SB

GO - Biological processi

  • BMP signaling pathway Source: UniProtKB
  • cartilage development Source: UniProtKB
  • cell differentiation Source: UniProtKB
  • cellular response to BMP stimulus Source: UniProtKB
  • cellular response to mechanical stimulus Source: UniProtKB
  • cellular response to transforming growth factor beta stimulus Source: UniProtKB
  • chondrocyte differentiation Source: UniProtKB
  • collagen fibril organization Source: UniProtKB
  • deltoid tuberosity development Source: UniProtKB
  • embryonic skeletal system development Source: UniProtKB
  • endochondral ossification Source: UniProtKB
  • face morphogenesis Source: UniProtKB
  • heart valve formation Source: UniProtKB
  • heart valve morphogenesis Source: UniProtKB
  • mesoderm formation Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of cartilage development Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of collagen biosynthetic process Source: UniProtKB
  • positive regulation of gastrulation Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of cartilage development Source: UniProtKB
  • sclerotome development Source: UniProtKB
  • Sertoli cell differentiation Source: UniProtKB
  • skeletal muscle cell differentiation Source: MGI
  • tendon cell differentiation Source: UniProtKB
  • tendon development Source: UniProtKB
  • tendon formation Source: UniProtKB
  • tissue homeostasis Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic helix-loop-helix transcription factor scleraxis
Gene namesi
Name:Scx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:102934. Scx.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274361 – 207Basic helix-loop-helix transcription factor scleraxisAdd BLAST207

Proteomic databases

PaxDbiQ64124.
PRIDEiQ64124.

PTM databases

iPTMnetiQ64124.
PhosphoSitePlusiQ64124.

Expressioni

Tissue specificityi

Expressed in mesenchymal precursors of cartilage and in connective tissue. Highly expressed in tendons in the limb, tongue and diaphragm and in cartilage of the bronchi.

Developmental stagei

At 6.0-6.5 dpc, expressed throughout the egg cylinder with highest expression in the epiblast. At 9.5-10.5 dpc, expressed in the lateral sclerotome and in mesenchymal cells of the limb buds and body wall. At 11.0 dpc, expressed in a metameric pattern extending along the length of the embryo. By 11.5 dpc, expression in the developing vertebrae and invertebral disks is extended caudally. High expression was seen in precursors of the ribs and bones of the limbs. Expression in progenitors of the ribs and the axial and appendicular skeleton becomes down-regulated when ossification is initiated.

Gene expression databases

BgeeiENSMUSG00000034161.
CleanExiMM_SCX.
ExpressionAtlasiQ64124. baseline and differential.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Dimerizes and binds the E-box consensus sequence with E12.

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi203115. 1 interactor.
STRINGi10090.ENSMUSP00000043668.

Structurei

3D structure databases

ProteinModelPortaliQ64124.
SMRiQ64124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 130bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi161 – 170Poly-Pro10

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000231706.
HOVERGENiHBG036149.
InParanoidiQ64124.
OMAiGQPCHTT.
OrthoDBiEOG091G0RR2.
PhylomeDBiQ64124.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFAMLRSAP PPGRYLYPEV SPLSEDEDRG SESSGSDEKP CRVHAARCGL
60 70 80 90 100
QGARRRAGGR RAAGSGPGPG GRPGREPRQR HTANARERDR TNSVNTAFTA
110 120 130 140 150
LRTLIPTEPA DRKLSKIETL RLASSYISHL GNVLLVGEAC GDGQPCHSGP
160 170 180 190 200
AFFHSGRAGS PLPPPPPPPP LARDGGENTQ PKQICTFCLS NQRKLSKDRD

RKTAIRS
Length:207
Mass (Da):22,239
Last modified:November 1, 1996 - v1
Checksum:i8340052200A85EF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78079 mRNA. Translation: AAB34266.1.
CCDSiCCDS27571.1.
RefSeqiNP_942588.1. NM_198885.3.
UniGeneiMm.322821.

Genome annotation databases

EnsembliENSMUST00000043089; ENSMUSP00000043668; ENSMUSG00000034161.
GeneIDi20289.
KEGGimmu:20289.
UCSCiuc007wkg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78079 mRNA. Translation: AAB34266.1.
CCDSiCCDS27571.1.
RefSeqiNP_942588.1. NM_198885.3.
UniGeneiMm.322821.

3D structure databases

ProteinModelPortaliQ64124.
SMRiQ64124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203115. 1 interactor.
STRINGi10090.ENSMUSP00000043668.

PTM databases

iPTMnetiQ64124.
PhosphoSitePlusiQ64124.

Proteomic databases

PaxDbiQ64124.
PRIDEiQ64124.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043089; ENSMUSP00000043668; ENSMUSG00000034161.
GeneIDi20289.
KEGGimmu:20289.
UCSCiuc007wkg.2. mouse.

Organism-specific databases

CTDi642658.
MGIiMGI:102934. Scx.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000231706.
HOVERGENiHBG036149.
InParanoidiQ64124.
OMAiGQPCHTT.
OrthoDBiEOG091G0RR2.
PhylomeDBiQ64124.
TreeFamiTF315153.

Miscellaneous databases

PROiQ64124.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034161.
CleanExiMM_SCX.
ExpressionAtlasiQ64124. baseline and differential.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCX_MOUSE
AccessioniPrimary (citable) accession number: Q64124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.