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Protein

Interferon-induced protein with tetratricopeptide repeats 2

Gene

Ifit2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to interferon-alpha Source: MGI
  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • negative regulation of protein binding Source: MGI
  • positive regulation of apoptotic process Source: UniProtKB
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Apoptosis, Immunity, Innate immunity

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced protein with tetratricopeptide repeats 2
Short name:
IFIT-2
Alternative name(s):
Glucocorticoid-attenuated response gene 39 protein
Short name:
GARG-39
Interferon-induced 54 kDa protein
Short name:
IFI-54K
Short name:
P54
Gene namesi
Name:Ifit2
Synonyms:Garg39, Ifi54, Isg54
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99449. Ifit2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 472471Interferon-induced protein with tetratricopeptide repeats 2PRO_0000106348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ64112.
MaxQBiQ64112.
PaxDbiQ64112.
PRIDEiQ64112.

PTM databases

PhosphoSiteiQ64112.

Expressioni

Inductioni

By type I interferons, dsRNAs and viruses.1 Publication

Interactioni

Subunit structurei

Domain-swapped homodimer. Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT1 and IFIT3. Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1 (By similarity). Interacts with EIF3C.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099890.

Structurei

3D structure databases

ProteinModelPortaliQ64112.
SMRiQ64112. Positions 8-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati51 – 8939TPR 1Add
BLAST
Repeati90 – 13546TPR 2Add
BLAST
Repeati136 – 17136TPR 3Add
BLAST
Repeati172 – 20837TPR 4Add
BLAST
Repeati244 – 27734TPR 5Add
BLAST
Repeati278 – 33356TPR 6Add
BLAST
Repeati334 – 36431TPR 7Add
BLAST
Repeati365 – 40339TPR 8Add
BLAST
Repeati404 – 44542TPR 9Add
BLAST

Domaini

The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface.By similarity

Sequence similaritiesi

Belongs to the IFIT family.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
HOGENOMiHOG000001558.
HOVERGENiHBG066330.
InParanoidiQ64112.
PhylomeDBiQ64112.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024124. Interferon-induced_IFIT2.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF4. PTHR10271:SF4. 1 hit.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTSKESLV CNLRQLKCHF TWNLIAEDES LDEFEDRVFN KDEFQNSEFK
60 70 80 90 100
ATMCNILAYV KHCRGLNEAA LQCLGEAEGF IQQQHPDQVE IRSLVTWGNY
110 120 130 140 150
AWVYYHMGQF SKAQAYLDKV KQVCKKFSSP YRIENPALDC EEGWARLKCT
160 170 180 190 200
KNQNERVKVC FQKALEKDPK NPEFTSGWAI AFYRLDDWPA RNYCIDSLEQ
210 220 230 240 250
AIQLSPDNTY VKVLLALKLD AVHVHKNQAM ALVEEALKKD PSAIDTLLRA
260 270 280 290 300
ARFYCKVYDT DRAIQLLRKA LEKLPNNAYV HYYMGCCYRS KVHHMLNRRE
310 320 330 340 350
MVFSGDRKKL EELIQLAVNH LRKAEEIKEM LEYSCSFLAD LYIIAKKYDE
360 370 380 390 400
ADYYFQKELS KDLPPGPKQL LHLRYGNFQF FQMKRQDKAI YHYMEGVKIK
410 420 430 440 450
KKTIPQKKMR EKLQRIALRR LHEDESDSEA LHILAFLQEN GGGQQADKDS
460 470
ERGVDSANQV PSASLDEDGA EY
Length:472
Mass (Da):55,021
Last modified:November 1, 1996 - v1
Checksum:i0C76034B1C0B2C41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112VC → ES in AAC52626 (PubMed:8660659).Curated
Sequence conflicti182 – 1821F → N in AAC52626 (PubMed:8660659).Curated
Sequence conflicti223 – 2242Missing in AAC52626 (PubMed:8660659).Curated
Sequence conflicti468 – 4681D → A in AAC52626 (PubMed:8660659).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77713, S77710 Genomic DNA. Translation: AAB33830.1.
U43085 mRNA. Translation: AAC52626.1.
PIRiA55508.
S71355.
RefSeqiNP_032358.1. NM_008332.3.
XP_006526768.1. XM_006526705.2.
XP_006526769.1. XM_006526706.2.
UniGeneiMm.2036.

Genome annotation databases

GeneIDi15958.
KEGGimmu:15958.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77713, S77710 Genomic DNA. Translation: AAB33830.1.
U43085 mRNA. Translation: AAC52626.1.
PIRiA55508.
S71355.
RefSeqiNP_032358.1. NM_008332.3.
XP_006526768.1. XM_006526705.2.
XP_006526769.1. XM_006526706.2.
UniGeneiMm.2036.

3D structure databases

ProteinModelPortaliQ64112.
SMRiQ64112. Positions 8-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099890.

PTM databases

PhosphoSiteiQ64112.

Proteomic databases

EPDiQ64112.
MaxQBiQ64112.
PaxDbiQ64112.
PRIDEiQ64112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi15958.
KEGGimmu:15958.

Organism-specific databases

CTDi3433.
MGIiMGI:99449. Ifit2.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
HOGENOMiHOG000001558.
HOVERGENiHBG066330.
InParanoidiQ64112.
PhylomeDBiQ64112.

Miscellaneous databases

ChiTaRSiIfit2. mouse.
PROiQ64112.
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024124. Interferon-induced_IFIT2.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF4. PTHR10271:SF4. 1 hit.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, chromosome localization, and regulation of expression of the interferon-regulated mouse Ifi54/Ifi56 gene family."
    Bluyssen H.A., Vlietstra R.J., Faber P.W., Smit E.M., Hagemeijer A., Trapman J.
    Genomics 24:137-148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-49/IRG2 encode inducible proteins containing multiple tetratricopeptide repeat domains."
    Smith J.B., Herschman H.R.
    Arch. Biochem. Biophys. 330:290-300(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Induction and mode of action of the viral stress-inducible murine proteins, P56 and P54."
    Terenzi F., Pal S., Sen G.C.
    Virology 340:116-124(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, INTERACTION WITH EIF3C.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart and Lung.
  5. Cited for: FUNCTION.
  6. Cited for: REVIEW.
  7. Cited for: FUNCTION.

Entry informationi

Entry nameiIFIT2_MOUSE
AccessioniPrimary (citable) accession number: Q64112
Secondary accession number(s): Q62385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.