Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sepiapterin reductase

Gene

Spr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.

Catalytic activityi

L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH.
L-erythro-tetrahydrobiopterin + 2 NADP+ = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171Substrate1
Binding sitei175NADP1
Binding sitei200Substrate; via amide nitrogen1
Binding sitei222Substrate1
Binding sitei258Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 21NADP7
Nucleotide bindingi43 – 44NADP2
Nucleotide bindingi70 – 71NADP2
Nucleotide bindingi202 – 207NADP6

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • sepiapterin reductase activity Source: UniProtKB

GO - Biological processi

  • cell morphogenesis involved in neuron differentiation Source: MGI
  • dopamine metabolic process Source: MGI
  • L-phenylalanine metabolic process Source: MGI
  • nitric oxide biosynthetic process Source: MGI
  • norepinephrine metabolic process Source: MGI
  • pteridine metabolic process Source: MGI
  • regulation of multicellular organism growth Source: MGI
  • serotonin metabolic process Source: MGI
  • tetrahydrobiopterin biosynthetic process Source: InterPro
  • tetrahydrobiopterin metabolic process Source: MGI
  • voluntary musculoskeletal movement Source: MGI

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.153 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Sepiapterin reductase (EC:1.1.1.153)
Short name:
SPR
Gene namesi
Name:Spr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103078 Spr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721501 – 261Sepiapterin reductaseAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei33PhosphoserineBy similarity1
Modified residuei46PhosphoserineBy similarity1
Modified residuei196PhosphoserineBy similarity1
Modified residuei214PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ64105
MaxQBiQ64105
PaxDbiQ64105
PeptideAtlasiQ64105
PRIDEiQ64105

2D gel databases

REPRODUCTION-2DPAGEQ64105

PTM databases

iPTMnetiQ64105
PhosphoSitePlusiQ64105
SwissPalmiQ64105

Expressioni

Gene expression databases

CleanExiMM_SPR

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048111

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi19 – 29Combined sources11
Beta strandi37 – 43Combined sources7
Helixi45 – 58Combined sources14
Beta strandi62 – 68Combined sources7
Helixi74 – 86Combined sources13
Beta strandi95 – 100Combined sources6
Helixi112 – 114Combined sources3
Helixi118 – 128Combined sources11
Helixi130 – 141Combined sources12
Beta strandi150 – 156Combined sources7
Helixi159 – 161Combined sources3
Helixi169 – 188Combined sources20
Beta strandi192 – 198Combined sources7
Beta strandi201 – 204Combined sources4
Helixi205 – 213Combined sources9
Helixi217 – 228Combined sources12
Helixi235 – 248Combined sources14
Beta strandi255 – 258Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NASX-ray2.10A3-261[»]
1OAAX-ray1.25A3-261[»]
1SEPX-ray1.95A1-261[»]
ProteinModelPortaliQ64105
SMRiQ64105
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64105

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 159Substrate binding2

Sequence similaritiesi

Belongs to the sepiapterin reductase family.Curated

Phylogenomic databases

eggNOGiKOG1204 Eukaryota
ENOG4111PZG LUCA
HOVERGENiHBG006973
InParanoidiQ64105

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
IPR006393 Sepiapterin_red
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01500 sepiapter_red, 1 hit

Sequencei

Sequence statusi: Complete.

Q64105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADGLGCAV CVLTGASRGF GRALAPQLAR LLSPGSVMLV SARSESMLRQ
60 70 80 90 100
LKEELGAQQP DLKVVLAAAD LGTEAGVQRL LSAVRELPRP EGLQRLLLIN
110 120 130 140 150
NAATLGDVSK GFLNVNDLAE VNNYWALNLT SMLCLTSGTL NAFQDSPGLS
160 170 180 190 200
KTVVNISSLC ALQPYKGWGL YCAGKAARDM LYQVLAAEEP SVRVLSYAPG
210 220 230 240 250
PLDNDMQQLA RETSKDPELR SKLQKLKSDG ALVDCGTSAQ KLLGLLQKDT
260
FQSGAHVDFY D
Length:261
Mass (Da):27,883
Last modified:November 1, 1997 - v1
Checksum:i102294E439CB8AEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4D → G in AAC69364 (PubMed:10209270).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77493 mRNA Translation: AAB33611.1
U78077, U78076 Genomic DNA Translation: AAC69364.1
S71375 mRNA No translation available.
PIRiS52110
UniGeneiMm.28393

Similar proteinsi

Entry informationi

Entry nameiSPRE_MOUSE
AccessioniPrimary (citable) accession number: Q64105
Secondary accession number(s): Q63996
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health