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Protein

Adipocyte enhancer-binding protein 1

Gene

Aebp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 2 may positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. Isoform 2 may also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.9 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Calmodulin-binding, DNA-binding

Protein family/group databases

MEROPSiM14.951.

Names & Taxonomyi

Protein namesi
Recommended name:
Adipocyte enhancer-binding protein 1
Short name:
AE-binding protein 1
Alternative name(s):
Aortic carboxypeptidase-like protein
Gene namesi
Name:Aebp1
Synonyms:Aclp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1197012. Aebp1.

Subcellular locationi

Isoform 1 :
Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Disruption phenotypei

Two independent knockout mice have been generated for the gene encoding this protein, and these exhibit different phenotypes. Mice lacking exons 7-12 exhibit reduced growth rate and body weight and resistance to dietary-induced obesity. Individual adipocytes from these animals are hypoproliferative while the adipose tissue is prone to apoptosis. Mice lacking exons 7-16 die perinatally from gastroschisis, in which abdominal viscera are extruded through the ventral body wall. Surviving mice exhibit deficient wound healing, having dermal fibroblasts with reduced proliferative capacity. Mice lacking exons 7-16 may exhibit phenotypes arising from effects on the locus encoding Pold2, which lies immediately downstream of this locus.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1003 – 10031T → A: Impairs DNA-binding and phosphorylation by MAPK1. 1 Publication
Mutagenesisi1003 – 10031T → D: Impairs DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 11281103Adipocyte enhancer-binding protein 1PRO_0000333190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated by MAPK1 in vitro.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ640N1.
PaxDbiQ640N1.
PeptideAtlasiQ640N1.
PRIDEiQ640N1.

PTM databases

iPTMnetiQ640N1.
PhosphoSiteiQ640N1.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in adipose tissue, brain, heart, kidney, liver, lung, skeletal muscle, small intestine, spleen and testis. Isoform 2 is expressed in macrophages. Expressed in aorta, preadipocytes, adipocyte tissue, brain, heart, liver, lung, skeletal muscle, skin and spleen (at protein level).3 Publications

Developmental stagei

Expressed in the somites and dorsa from E9.5 and in the yolk sac and embryonic vasculature from E10.5. Expression within the large and small blood vessels increases at E11.5 and E13.5, with high expression in the vascular smooth muscle at E16.5. Also expressed later in development in mesenchymal cells in the dermal layer, the developing skeleton, connective tissue and the umbilical ring and vessels. Up-regulated during vascular smooth muscle cell differentiation and down-regulated during adipocyte differentiation and osteoblast differentiation.9 Publications

Inductioni

By TGF-beta. Expression is also induced by a high fat diet.2 Publications

Gene expression databases

BgeeiENSMUSG00000020473.
GenevisibleiQ640N1. MM.

Interactioni

Subunit structurei

Isoform 2 interacts with GNG5, NFKBIA, MAPK1, MAPK3 and PTEN. Interaction with MAPK1 may stimulate DNA-binding. May interact with calmodulin. Binds to DNA in vitro.5 Publications

Protein-protein interaction databases

BioGridi198013. 2 interactions.
STRINGi10090.ENSMUSP00000099987.

Structurei

3D structure databases

ProteinModelPortaliQ640N1.
SMRiQ640N1. Positions 378-531, 551-976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini374 – 531158F5/8 type CPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 546166Required for DNA-binding and interaction with NFKBIAAdd
BLAST
Regioni412 – 615204Interaction with MAPK1 and MAPK3Add
BLAST
Regioni546 – 976431Interaction with PTENAdd
BLAST
Regioni932 – 1128197Required for transcriptional repressionAdd
BLAST
Regioni997 – 1128132Interaction with MAPK1 and MAPK3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi70 – 16697Lys-richAdd
BLAST
Compositional biasi625 – 6295Poly-Leu
Compositional biasi1069 – 110638Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOVERGENiHBG003410.
InParanoidiQ640N1.
OMAiTLDYNDQ.
OrthoDBiEOG091G06A9.
PhylomeDBiQ640N1.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q640N1-1) [UniParc]FASTAAdd to basket
Also known as: Aclp

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPVRTASLL CGLLALLTLC PEGNPQTVLT DDEIEEFLEG FLSELETQSP
60 70 80 90 100
PREDDVEVQP LPEPTQRPRK SKAGGKQRAD VEVPPEKNKD KEKKGKKDKG
110 120 130 140 150
PKATKPLEGS TRPTKKPKEK PPKATKKPKE KPPKATKKPK EKPPKATKKP
160 170 180 190 200
KEKPPKATKR PSAGKKFSTV APLETLDRLL PSPSNPSAQE LPQKRDTPFP
210 220 230 240 250
NAWQGQGEET QVEAKQPRPE PEEETEMPTL DYNDQIEKED YEDFEYIRRQ
260 270 280 290 300
KQPRPTPSRR RLWPERPEEK TEEPEERKEV EPPLKPLLPP DYGDSYVIPN
310 320 330 340 350
YDDLDYYFPH PPPQKPDVGQ EVDEEKEEMK KPKKEGSSPK EDTEDKWTVE
360 370 380 390 400
KNKDHKGPRK GEELEEEWAP VEKIKCPPIG MESHRIEDNQ IRASSMLRHG
410 420 430 440 450
LGAQRGRLNM QAGANEDDYY DGAWCAEDES QTQWIEVDTR RTTRFTGVIT
460 470 480 490 500
QGRDSSIHDD FVTTFFVGFS NDSQTWVMYT NGYEEMTFYG NVDKDTPVLS
510 520 530 540 550
ELPEPVVARF IRIYPLTWNG SLCMRLEVLG CPVTPVYSYY AQNEVVTTDS
560 570 580 590 600
LDFRHHSYKD MRQLMKAVNE ECPTITRTYS LGKSSRGLKI YAMEISDNPG
610 620 630 640 650
DHELGEPEFR YTAGIHGNEV LGRELLLLLM QYLCQEYRDG NPRVRNLVQD
660 670 680 690 700
TRIHLVPSLN PDGYEVAAQM GSEFGNWALG LWTEEGFDIF EDFPDLNSVL
710 720 730 740 750
WAAEEKKWVP YRVPNNNLPI PERYLSPDAT VSTEVRAIIS WMEKNPFVLG
760 770 780 790 800
ANLNGGERLV SYPYDMARTP SQEQLLAEAL AAARGEDDDG VSEAQETPDH
810 820 830 840 850
AIFRWLAISF ASAHLTMTEP YRGGCQAQDY TSGMGIVNGA KWNPRSGTFN
860 870 880 890 900
DFSYLHTNCL ELSVYLGCDK FPHESELPRE WENNKEALLT FMEQVHRGIK
910 920 930 940 950
GVVTDEQGIP IANATISVSG INHGVKTASG GDYWRILNPG EYRVTAHAEG
960 970 980 990 1000
YTSSAKICNV DYDIGATQCN FILARSNWKR IREILAMNGN RPILRVDPSR
1010 1020 1030 1040 1050
PMTPQQRRMQ QRRLQYRLRM REQMRLRRLN STAGPATSPT PALMPPPSPT
1060 1070 1080 1090 1100
PAITLRPWEV LPTTTAGWEE SETETYTEVV TEFETEYGTD LEVEEIEEEE
1110 1120
EEEEEEMDTG LTFPLTTVET YTVNFGDF
Length:1,128
Mass (Da):128,365
Last modified:October 25, 2004 - v1
Checksum:iC24F46B893C02DC9
GO
Isoform 2 (identifier: Q640N1-2) [UniParc]FASTAAdd to basket
Also known as: Aebp1

The sequence of this isoform differs from the canonical sequence as follows:
     1-380: Missing.

Note: No experimental confirmation available.
Show »
Length:748
Mass (Da):84,983
Checksum:iD27B68FD02F72A2E
GO

Sequence cautioni

The sequence CAA56648 differs from that shown. Reason: Frameshift at position 406. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti333 – 3331K → E in BAE34995 (PubMed:16141072).Curated
Sequence conflicti333 – 3331K → E in BAE35004 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE35359 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE35513 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE34995 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE35004 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE35033 (PubMed:16141072).Curated
Sequence conflicti356 – 3561K → KA in BAE35060 (PubMed:16141072).Curated
Sequence conflicti792 – 7921S → F in BAE35359 (PubMed:16141072).Curated
Sequence conflicti792 – 7921S → F in BAE35513 (PubMed:16141072).Curated
Sequence conflicti917 – 9171S → C in BAE35359 (PubMed:16141072).Curated
Sequence conflicti995 – 9951R → G in AAC25584 (PubMed:9624159).Curated
Sequence conflicti1096 – 10961I → IEE in BAE35359 (PubMed:16141072).Curated
Sequence conflicti1096 – 10961I → IEE in BAE35513 (PubMed:16141072).Curated
Sequence conflicti1096 – 10961I → IEE in BAE34995 (PubMed:16141072).Curated
Sequence conflicti1096 – 10961I → IEE in BAE35004 (PubMed:16141072).Curated
Sequence conflicti1096 – 10961I → IEE in BAE35033 (PubMed:16141072).Curated
Sequence conflicti1096 – 10961I → IEE in BAE35060 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE35359 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE35513 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE34995 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE35004 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE35033 (PubMed:16141072).Curated
Sequence conflicti1115 – 11151L → F in BAE35060 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 380380Missing in isoform 2. 1 PublicationVSP_033470Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80478 mRNA. Translation: CAA56648.1. Frameshift.
AF053943 mRNA. Translation: AAC25584.1.
AK159330 mRNA. Translation: BAE34995.1.
AK159342 mRNA. Translation: BAE35004.1.
AK159377 mRNA. Translation: BAE35033.1.
AK159409 mRNA. Translation: BAE35060.1.
AK159774 mRNA. Translation: BAE35359.1.
AK159957 mRNA. Translation: BAE35513.1.
AL627069 Genomic DNA. Translation: CAI25440.1.
AL627069 Genomic DNA. Translation: CAI25441.1.
BC082577 mRNA. Translation: AAH82577.1.
CCDSiCCDS24406.1. [Q640N1-1]
PIRiS60227. S51739.
RefSeqiNP_001278786.1. NM_001291857.2.
NP_033766.2. NM_009636.3. [Q640N1-1]
UniGeneiMm.3317.
Mm.4665.

Genome annotation databases

EnsembliENSMUST00000102923; ENSMUSP00000099987; ENSMUSG00000020473. [Q640N1-1]
ENSMUST00000109829; ENSMUSP00000105454; ENSMUSG00000020473. [Q640N1-2]
GeneIDi11568.
KEGGimmu:11568.
UCSCiuc007hxg.3. mouse. [Q640N1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80478 mRNA. Translation: CAA56648.1. Frameshift.
AF053943 mRNA. Translation: AAC25584.1.
AK159330 mRNA. Translation: BAE34995.1.
AK159342 mRNA. Translation: BAE35004.1.
AK159377 mRNA. Translation: BAE35033.1.
AK159409 mRNA. Translation: BAE35060.1.
AK159774 mRNA. Translation: BAE35359.1.
AK159957 mRNA. Translation: BAE35513.1.
AL627069 Genomic DNA. Translation: CAI25440.1.
AL627069 Genomic DNA. Translation: CAI25441.1.
BC082577 mRNA. Translation: AAH82577.1.
CCDSiCCDS24406.1. [Q640N1-1]
PIRiS60227. S51739.
RefSeqiNP_001278786.1. NM_001291857.2.
NP_033766.2. NM_009636.3. [Q640N1-1]
UniGeneiMm.3317.
Mm.4665.

3D structure databases

ProteinModelPortaliQ640N1.
SMRiQ640N1. Positions 378-531, 551-976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198013. 2 interactions.
STRINGi10090.ENSMUSP00000099987.

Protein family/group databases

MEROPSiM14.951.

PTM databases

iPTMnetiQ640N1.
PhosphoSiteiQ640N1.

Proteomic databases

MaxQBiQ640N1.
PaxDbiQ640N1.
PeptideAtlasiQ640N1.
PRIDEiQ640N1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102923; ENSMUSP00000099987; ENSMUSG00000020473. [Q640N1-1]
ENSMUST00000109829; ENSMUSP00000105454; ENSMUSG00000020473. [Q640N1-2]
GeneIDi11568.
KEGGimmu:11568.
UCSCiuc007hxg.3. mouse. [Q640N1-1]

Organism-specific databases

CTDi165.
MGIiMGI:1197012. Aebp1.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOVERGENiHBG003410.
InParanoidiQ640N1.
OMAiTLDYNDQ.
OrthoDBiEOG091G06A9.
PhylomeDBiQ640N1.
TreeFamiTF315592.

Miscellaneous databases

ChiTaRSiAebp1. mouse.
PROiQ640N1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020473.
GenevisibleiQ640N1. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. FA58C.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAEBP1_MOUSE
AccessioniPrimary (citable) accession number: Q640N1
Secondary accession number(s): O88442
, Q3TVV5, Q3TWA7, Q3TX56, Q3TXB2, Q5NCI9, Q61281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although related to peptidase M14 family, lacks the active sites residues and zinc-binding sites, suggesting that it has no carboxypeptidase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.