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Protein

OTU domain-containing protein 5-B

Gene

otud5-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Sequence Analysis
Active sitei182 – 1821NucleophileBy similarity
Active sitei287 – 2871By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked deubiquitination Source: UniProtKB
  2. protein K63-linked deubiquitination Source: UniProtKB
  3. response to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5-B (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:otud5-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6255335. otud5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518OTU domain-containing protein 5-BPRO_0000278228Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ640H3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 294124OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1827Cys-loopBy similarity
Regioni231 – 24111Variable-loopBy similarityAdd
BLAST
Regioni282 – 2876His-loopBy similarity

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG060214.
KOiK12655.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q640H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILPKKKPP PSSDPEGNGE RSGSGAPDSH SRSGARPRSS PPPRWAYPGN
60 70 80 90 100
PSSAAERHTQ QVSPPPGSAT SGGAGPLGDG APASSSSSSS SSCNGAGAGG
110 120 130 140 150
CCSGPGHSKR RRQVLSAGPG ATGNCPDTDD GAGNNSEDEY ETAAQTQHLD
160 170 180 190 200
PDTAQQQEHW FEKALCEKKG FIIKQMKEDG ACLFRAVADQ VYGDQDMHEV
210 220 230 240 250
VRKHCMDYLM KNADYFSNYV TEDFTTYINR KRKNNCHGNH IEMQAMAEMY
260 270 280 290 300
NRPVEVYQYG TEPINTFHGI QQNEDEPIRV SYHRNIHYNS VVNPNKATIG
310 320 330 340 350
VGLGLPSFKP GHAEQSLMKS AIRTSEESWI EQQMLEDKKR ATDWEATNEA
360 370 380 390 400
IEEQVARESY LQWLRDQEKQ ARQPRKASAT CSSATAAACS GLEEWSGRSP
410 420 430 440 450
RQRSTAGSPE QPDVHAELCM KPPSPGAALT LGKPPSPCAP GPSNQMSAGA
460 470 480 490 500
DRATSPLVSL YPALECRAIM QHMSPTAFGL KDWDDDEILA SVLAASQQEY
510
LDTMKKSTLR RESSPDHS
Length:518
Mass (Da):56,209
Last modified:October 25, 2004 - v1
Checksum:i2099AB07C6C93EC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082654 mRNA. Translation: AAH82654.1.
RefSeqiNP_001088023.1. NM_001094554.1.
UniGeneiXl.49149.

Genome annotation databases

GeneIDi494714.
KEGGixla:494714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082654 mRNA. Translation: AAH82654.1.
RefSeqiNP_001088023.1. NM_001094554.1.
UniGeneiXl.49149.

3D structure databases

ProteinModelPortaliQ640H3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC85.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi494714.
KEGGixla:494714.

Organism-specific databases

CTDi494714.
XenbaseiXB-GENE-6255335. otud5.

Phylogenomic databases

HOVERGENiHBG060214.
KOiK12655.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiOTU5B_XENLA
AccessioniPrimary (citable) accession number: Q640H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 25, 2004
Last modified: October 1, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.