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Protein

Transcription factor E3

Gene

Tfe3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.1 Publication

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • humoral immune response Source: UniProtKB
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of osteoclast differentiation Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAdaptive immunity, Immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E3
Gene namesi
Name:Tfe3
Synonyms:Tcfe3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:98511. Tfe3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274721 – 572Transcription factor E3Add BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187Asymmetric dimethylarginineCombined sources1
Cross-linki338Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei539PhosphoserineBy similarity1
Modified residuei545PhosphoserineCombined sources1
Modified residuei551PhosphoserineCombined sources1
Modified residuei553PhosphoserineCombined sources1
Modified residuei557PhosphoserineCombined sources1
Modified residuei565PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated; does not affect dimerization with MITF.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ64092.
PRIDEiQ64092.

PTM databases

iPTMnetiQ64092.
PhosphoSitePlusiQ64092.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000000134.
CleanExiMM_TCFE3.
ExpressionAtlasiQ64092. baseline and differential.
GenevisibleiQ64092. MM.

Interactioni

Subunit structurei

Homodimer and heterodimer; with TFEB or MITF.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi229078. 1 interactor.
IntActiQ64092. 1 interactor.
STRINGi10090.ENSMUSP00000076864.

Structurei

3D structure databases

ProteinModelPortaliQ64092.
SMRiQ64092.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini345 – 398bHLHPROSITE-ProRule annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 270Strong transcription activation domainSequence analysisAdd BLAST12
Regioni408 – 429Leucine-zipperAdd BLAST22

Sequence similaritiesi

Belongs to the MiT/TFE family.Curated

Phylogenomic databases

eggNOGiKOG1318. Eukaryota.
ENOG410ZYYV. LUCA.
GeneTreeiENSGT00390000004402.
HOVERGENiHBG006768.
InParanoidiQ64092.
KOiK09105.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR021802. MiT/TFE_C.
IPR031867. MiT/TFE_N.
IPR024100. TFE3.
PANTHERiPTHR12565:SF263. PTHR12565:SF263. 1 hit.
PfamiView protein in Pfam
PF11851. DUF3371. 1 hit.
PF00010. HLH. 1 hit.
PF15951. MITF_TFEB_C_3_N. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64092-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHAAEPARD AVEASAEGPR AVFLLLEERR PAESAQLLSL NSLLPESGIV
60 70 80 90 100
ADIELENILD PDSFYELKSQ PLFLRSSLPI SLQATPTTPA TLSASSSAGG
110 120 130 140 150
SRTPAMSSSS SRVLLRQQLM RAQAQEQERR ERREQAAAAP FPSPAPASPA
160 170 180 190 200
ISVIGVSAGG HTLSRPPPAQ VPREVLKVQT HLENPTRYHL QQARRQQVKQ
210 220 230 240 250
YLSTTLGPKL ASQALTPPPG PSSAQPLPAP ETAHATGPTG SAPNSPMALL
260 270 280 290 300
TIGSSSEKEI DDVIDEIISL ESSYNDEMLS YLPGGTAGLQ LPSTLPVSGN
310 320 330 340 350
LLDVYSSQGV ATPAITVSNS CPAELPNIKR EISETEAKAL LKERQKKDNH
360 370 380 390 400
NLIERRRRFN INDRIKELGT LIPKSNDPEM RWNKGTILKA SVDYIRKLQK
410 420 430 440 450
EQQRSKDLES RQRSLEQANR SLQLRIQELE LQAQIHGLPV PPNPGLLSLT
460 470 480 490 500
TSSVSDSLKP EQLDIEEEGR PSTTFHVSGG PAQNAPPQQP PAPPSDALLD
510 520 530 540 550
LHFPSDHLGD LGDPFHLGLE DILMEEEGMV GGLSGGALSP LRAASDPLLS
560 570
SVSPAVSKAS SRRSSFSMEE ES
Length:572
Mass (Da):61,536
Last modified:January 9, 2007 - v2
Checksum:iDAB0487EC0F6E92D
GO
Isoform 2 (identifier: Q64092-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Note: No experimental confirmation available.
Show »
Length:467
Mass (Da):50,509
Checksum:i2353472086F2A3A0
GO
Isoform 3 (identifier: Q64092-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     260-294: Missing.

Note: No experimental confirmation available.
Show »
Length:432
Mass (Da):46,770
Checksum:i24EE10757C31E2CC
GO
Isoform 4 (identifier: Q64092-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-294: Missing.

Note: No experimental confirmation available.
Show »
Length:537
Mass (Da):57,797
Checksum:i8E063275FBFC4F7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73F → S in BAE24128 (PubMed:16141072).Curated1
Sequence conflicti73F → S in BAE25994 (PubMed:16141072).Curated1
Sequence conflicti127 – 131QERRE → TSGTR in AAB21130 (PubMed:1732746).Curated5
Sequence conflicti143S → G in BAE25994 (PubMed:16141072).Curated1
Sequence conflicti558K → N in AAB21130 (PubMed:1732746).Curated1
Sequence conflicti568M → I in AAB21130 (PubMed:1732746).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0221431 – 105Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_022144260 – 294Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089762 mRNA. Translation: BAC40955.1.
AK139756 mRNA. Translation: BAE24128.1.
AK144657 mRNA. Translation: BAE25994.1.
AK152221 mRNA. Translation: BAE31048.1.
AL671995 Genomic DNA. Translation: CAM13597.1.
AL671995 Genomic DNA. Translation: CAM13598.1.
BC056358 mRNA. Translation: AAH56358.1.
BC063047 mRNA. Translation: AAH63047.1.
S76673 mRNA. Translation: AAB21130.1.
CCDSiCCDS52984.1. [Q64092-1]
CCDS52985.1. [Q64092-4]
CCDS72332.1. [Q64092-2]
CCDS72333.1. [Q64092-3]
PIRiA42029.
RefSeqiNP_001098666.1. NM_001105196.1.
NP_001098667.1. NM_001105197.1. [Q64092-2]
NP_001258418.1. NM_001271489.1. [Q64092-2]
NP_001258419.1. NM_001271490.1. [Q64092-2]
NP_001258420.1. NM_001271491.1. [Q64092-3]
NP_766060.2. NM_172472.3.
XP_006527641.1. XM_006527578.2. [Q64092-2]
XP_017173942.1. XM_017318453.1. [Q64092-2]
XP_017173943.1. XM_017318454.1. [Q64092-3]
UniGeneiMm.249142.

Genome annotation databases

EnsembliENSMUST00000101695; ENSMUSP00000099219; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115677; ENSMUSP00000111341; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115678; ENSMUSP00000111342; ENSMUSG00000000134. [Q64092-3]
ENSMUST00000115679; ENSMUSP00000111343; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000137467; ENSMUSP00000134125; ENSMUSG00000000134. [Q64092-2]
GeneIDi209446.
KEGGimmu:209446.
UCSCiuc009smg.2. mouse. [Q64092-1]
uc009smk.2. mouse. [Q64092-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTFE3_MOUSE
AccessioniPrimary (citable) accession number: Q64092
Secondary accession number(s): A2AEW3
, A2AEW4, Q3U8H0, Q3UMV4, Q3UT52, Q7TNC1, Q8BN29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2007
Last modified: August 30, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families