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Protein

Transcription factor E3

Gene

Tfe3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.1 Publication

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein heterodimerization activity Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  4. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. humoral immune response Source: UniProtKB
  2. positive regulation of cell adhesion Source: UniProtKB
  3. positive regulation of transcription, DNA-templated Source: MGI
  4. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  5. regulation of osteoclast differentiation Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Adaptive immunity, Immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E3
Gene namesi
Name:Tfe3
Synonyms:Tcfe3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:98511. Tfe3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Transcription factor E3PRO_0000127472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei553 – 5531PhosphoserineBy similarity
Modified residuei557 – 5571PhosphoserineBy similarity
Modified residuei565 – 5651PhosphoserineBy similarity

Post-translational modificationi

Sumoylated; does not affect dimerization with MITF.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ64092.
PaxDbiQ64092.
PRIDEiQ64092.

PTM databases

PhosphoSiteiQ64092.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ64092.
CleanExiMM_TCFE3.
ExpressionAtlasiQ64092. baseline and differential.
GenevestigatoriQ64092.

Interactioni

Subunit structurei

Homodimer and heterodimer; with TFEB or MITF.By similarity

Protein-protein interaction databases

BioGridi229078. 1 interaction.
IntActiQ64092. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ64092.
SMRiQ64092. Positions 346-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini345 – 39854bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni259 – 27012Strong transcription activation domainSequence AnalysisAdd
BLAST
Regioni408 – 42922Leucine-zipperAdd
BLAST

Sequence similaritiesi

Belongs to the MiT/TFE family.Curated
Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG251286.
GeneTreeiENSGT00390000004402.
HOVERGENiHBG006768.
InParanoidiQ64092.
KOiK09105.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR021802. bHLH_ZIP_TF_MiT/TFE.
IPR024100. TFE3.
[Graphical view]
PANTHERiPTHR10014:SF37. PTHR10014:SF37. 1 hit.
PfamiPF11851. DUF3371. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64092-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHAAEPARD AVEASAEGPR AVFLLLEERR PAESAQLLSL NSLLPESGIV
60 70 80 90 100
ADIELENILD PDSFYELKSQ PLFLRSSLPI SLQATPTTPA TLSASSSAGG
110 120 130 140 150
SRTPAMSSSS SRVLLRQQLM RAQAQEQERR ERREQAAAAP FPSPAPASPA
160 170 180 190 200
ISVIGVSAGG HTLSRPPPAQ VPREVLKVQT HLENPTRYHL QQARRQQVKQ
210 220 230 240 250
YLSTTLGPKL ASQALTPPPG PSSAQPLPAP ETAHATGPTG SAPNSPMALL
260 270 280 290 300
TIGSSSEKEI DDVIDEIISL ESSYNDEMLS YLPGGTAGLQ LPSTLPVSGN
310 320 330 340 350
LLDVYSSQGV ATPAITVSNS CPAELPNIKR EISETEAKAL LKERQKKDNH
360 370 380 390 400
NLIERRRRFN INDRIKELGT LIPKSNDPEM RWNKGTILKA SVDYIRKLQK
410 420 430 440 450
EQQRSKDLES RQRSLEQANR SLQLRIQELE LQAQIHGLPV PPNPGLLSLT
460 470 480 490 500
TSSVSDSLKP EQLDIEEEGR PSTTFHVSGG PAQNAPPQQP PAPPSDALLD
510 520 530 540 550
LHFPSDHLGD LGDPFHLGLE DILMEEEGMV GGLSGGALSP LRAASDPLLS
560 570
SVSPAVSKAS SRRSSFSMEE ES
Length:572
Mass (Da):61,536
Last modified:January 9, 2007 - v2
Checksum:iDAB0487EC0F6E92D
GO
Isoform 2 (identifier: Q64092-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Note: No experimental confirmation available.

Show »
Length:467
Mass (Da):50,509
Checksum:i2353472086F2A3A0
GO
Isoform 3 (identifier: Q64092-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     260-294: Missing.

Note: No experimental confirmation available.

Show »
Length:432
Mass (Da):46,770
Checksum:i24EE10757C31E2CC
GO
Isoform 4 (identifier: Q64092-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-294: Missing.

Note: No experimental confirmation available.

Show »
Length:537
Mass (Da):57,797
Checksum:i8E063275FBFC4F7A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731F → S in BAE24128. (PubMed:16141072)Curated
Sequence conflicti73 – 731F → S in BAE25994. (PubMed:16141072)Curated
Sequence conflicti127 – 1315QERRE → TSGTR in AAB21130. (PubMed:1732746)Curated
Sequence conflicti143 – 1431S → G in BAE25994. (PubMed:16141072)Curated
Sequence conflicti558 – 5581K → N in AAB21130. (PubMed:1732746)Curated
Sequence conflicti568 – 5681M → I in AAB21130. (PubMed:1732746)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 105105Missing in isoform 2 and isoform 3. 1 PublicationVSP_022143Add
BLAST
Alternative sequencei260 – 29435Missing in isoform 3 and isoform 4. 1 PublicationVSP_022144Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089762 mRNA. Translation: BAC40955.1.
AK139756 mRNA. Translation: BAE24128.1.
AK144657 mRNA. Translation: BAE25994.1.
AK152221 mRNA. Translation: BAE31048.1.
AL671995 Genomic DNA. Translation: CAM13597.1.
AL671995 Genomic DNA. Translation: CAM13598.1.
BC056358 mRNA. Translation: AAH56358.1.
BC063047 mRNA. Translation: AAH63047.1.
S76673 mRNA. Translation: AAB21130.1.
CCDSiCCDS52984.1. [Q64092-1]
CCDS52985.1. [Q64092-4]
CCDS72332.1. [Q64092-2]
CCDS72333.1. [Q64092-3]
PIRiA42029.
RefSeqiNP_001098666.1. NM_001105196.1.
NP_001098667.1. NM_001105197.1. [Q64092-2]
NP_001258418.1. NM_001271489.1. [Q64092-2]
NP_001258419.1. NM_001271490.1. [Q64092-2]
NP_001258420.1. NM_001271491.1. [Q64092-3]
NP_766060.2. NM_172472.3.
XP_006527641.1. XM_006527578.1. [Q64092-2]
UniGeneiMm.249142.

Genome annotation databases

EnsembliENSMUST00000101695; ENSMUSP00000099219; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115677; ENSMUSP00000111341; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115678; ENSMUSP00000111342; ENSMUSG00000000134. [Q64092-3]
ENSMUST00000115679; ENSMUSP00000111343; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000137467; ENSMUSP00000134125; ENSMUSG00000000134. [Q64092-2]
GeneIDi209446.
KEGGimmu:209446.
UCSCiuc009smg.2. mouse. [Q64092-1]
uc009smk.2. mouse. [Q64092-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089762 mRNA. Translation: BAC40955.1.
AK139756 mRNA. Translation: BAE24128.1.
AK144657 mRNA. Translation: BAE25994.1.
AK152221 mRNA. Translation: BAE31048.1.
AL671995 Genomic DNA. Translation: CAM13597.1.
AL671995 Genomic DNA. Translation: CAM13598.1.
BC056358 mRNA. Translation: AAH56358.1.
BC063047 mRNA. Translation: AAH63047.1.
S76673 mRNA. Translation: AAB21130.1.
CCDSiCCDS52984.1. [Q64092-1]
CCDS52985.1. [Q64092-4]
CCDS72332.1. [Q64092-2]
CCDS72333.1. [Q64092-3]
PIRiA42029.
RefSeqiNP_001098666.1. NM_001105196.1.
NP_001098667.1. NM_001105197.1. [Q64092-2]
NP_001258418.1. NM_001271489.1. [Q64092-2]
NP_001258419.1. NM_001271490.1. [Q64092-2]
NP_001258420.1. NM_001271491.1. [Q64092-3]
NP_766060.2. NM_172472.3.
XP_006527641.1. XM_006527578.1. [Q64092-2]
UniGeneiMm.249142.

3D structure databases

ProteinModelPortaliQ64092.
SMRiQ64092. Positions 346-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229078. 1 interaction.
IntActiQ64092. 1 interaction.

PTM databases

PhosphoSiteiQ64092.

Proteomic databases

MaxQBiQ64092.
PaxDbiQ64092.
PRIDEiQ64092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101695; ENSMUSP00000099219; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115677; ENSMUSP00000111341; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000115678; ENSMUSP00000111342; ENSMUSG00000000134. [Q64092-3]
ENSMUST00000115679; ENSMUSP00000111343; ENSMUSG00000000134. [Q64092-2]
ENSMUST00000137467; ENSMUSP00000134125; ENSMUSG00000000134. [Q64092-2]
GeneIDi209446.
KEGGimmu:209446.
UCSCiuc009smg.2. mouse. [Q64092-1]
uc009smk.2. mouse. [Q64092-3]

Organism-specific databases

CTDi7030.
MGIiMGI:98511. Tfe3.

Phylogenomic databases

eggNOGiNOG251286.
GeneTreeiENSGT00390000004402.
HOVERGENiHBG006768.
InParanoidiQ64092.
KOiK09105.

Miscellaneous databases

NextBioi372667.
PROiQ64092.
SOURCEiSearch...

Gene expression databases

BgeeiQ64092.
CleanExiMM_TCFE3.
ExpressionAtlasiQ64092. baseline and differential.
GenevestigatoriQ64092.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR021802. bHLH_ZIP_TF_MiT/TFE.
IPR024100. TFE3.
[Graphical view]
PANTHERiPTHR10014:SF37. PTHR10014:SF37. 1 hit.
PfamiPF11851. DUF3371. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Egg, Lung and Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  4. "mTFE3, an X-linked transcriptional activator containing basic helix-loop-helix and zipper domains, utilizes the zipper to stabilize both DNA binding and multimerization."
    Roman C., Matera A.G., Cooper C., Artandi S., Blain S., Ward D.C., Calame K.
    Mol. Cell. Biol. 12:817-827(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-572.
  5. "Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity."
    Huan C., Kelly M.L., Steele R., Shapira I., Gottesman S.R.S., Roman C.A.J.
    Nat. Immunol. 7:1082-1091(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTFE3_MOUSE
AccessioniPrimary (citable) accession number: Q64092
Secondary accession number(s): A2AEW3
, A2AEW4, Q3U8H0, Q3UMV4, Q3UT52, Q7TNC1, Q8BN29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.