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Q64092

- TFE3_MOUSE

UniProt

Q64092 - TFE3_MOUSE

Protein

Transcription factor E3

Gene

Tfe3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein heterodimerization activity Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    4. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. humoral immune response Source: UniProtKB
    2. positive regulation of cell adhesion Source: UniProtKB
    3. positive regulation of transcription, DNA-templated Source: MGI
    4. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    5. regulation of osteoclast differentiation Source: MGI
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Adaptive immunity, Immunity, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E3
    Gene namesi
    Name:Tfe3
    Synonyms:Tcfe3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:98511. Tfe3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Transcription factor E3PRO_0000127472Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei545 – 5451PhosphoserineBy similarity
    Modified residuei553 – 5531PhosphoserineBy similarity
    Modified residuei557 – 5571PhosphoserineBy similarity
    Modified residuei565 – 5651PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylated; does not affect dimerization with MITF.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ64092.
    PRIDEiQ64092.

    PTM databases

    PhosphoSiteiQ64092.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    BgeeiQ64092.
    CleanExiMM_TCFE3.
    GenevestigatoriQ64092.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer; with TFEB or MITF.By similarity

    Protein-protein interaction databases

    BioGridi229078. 1 interaction.
    IntActiQ64092. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64092.
    SMRiQ64092. Positions 346-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini345 – 39854bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni259 – 27012Strong transcription activation domainSequence AnalysisAdd
    BLAST
    Regioni408 – 42922Leucine-zipperAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MiT/TFE family.Curated
    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG251286.
    GeneTreeiENSGT00390000004402.
    HOVERGENiHBG006768.
    InParanoidiA2AEW3.
    KOiK09105.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR021802. bHLH_ZIP_TF_MiT/TFE.
    IPR024100. TFE3.
    [Graphical view]
    PANTHERiPTHR10014:SF37. PTHR10014:SF37. 1 hit.
    PfamiPF11851. DUF3371. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64092-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSHAAEPARD AVEASAEGPR AVFLLLEERR PAESAQLLSL NSLLPESGIV    50
    ADIELENILD PDSFYELKSQ PLFLRSSLPI SLQATPTTPA TLSASSSAGG 100
    SRTPAMSSSS SRVLLRQQLM RAQAQEQERR ERREQAAAAP FPSPAPASPA 150
    ISVIGVSAGG HTLSRPPPAQ VPREVLKVQT HLENPTRYHL QQARRQQVKQ 200
    YLSTTLGPKL ASQALTPPPG PSSAQPLPAP ETAHATGPTG SAPNSPMALL 250
    TIGSSSEKEI DDVIDEIISL ESSYNDEMLS YLPGGTAGLQ LPSTLPVSGN 300
    LLDVYSSQGV ATPAITVSNS CPAELPNIKR EISETEAKAL LKERQKKDNH 350
    NLIERRRRFN INDRIKELGT LIPKSNDPEM RWNKGTILKA SVDYIRKLQK 400
    EQQRSKDLES RQRSLEQANR SLQLRIQELE LQAQIHGLPV PPNPGLLSLT 450
    TSSVSDSLKP EQLDIEEEGR PSTTFHVSGG PAQNAPPQQP PAPPSDALLD 500
    LHFPSDHLGD LGDPFHLGLE DILMEEEGMV GGLSGGALSP LRAASDPLLS 550
    SVSPAVSKAS SRRSSFSMEE ES 572
    Length:572
    Mass (Da):61,536
    Last modified:January 9, 2007 - v2
    Checksum:iDAB0487EC0F6E92D
    GO
    Isoform 2 (identifier: Q64092-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:467
    Mass (Da):50,509
    Checksum:i2353472086F2A3A0
    GO
    Isoform 3 (identifier: Q64092-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: Missing.
         260-294: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:432
    Mass (Da):46,770
    Checksum:i24EE10757C31E2CC
    GO
    Isoform 4 (identifier: Q64092-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         260-294: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:537
    Mass (Da):57,797
    Checksum:i8E063275FBFC4F7A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731F → S in BAE24128. (PubMed:16141072)Curated
    Sequence conflicti73 – 731F → S in BAE25994. (PubMed:16141072)Curated
    Sequence conflicti127 – 1315QERRE → TSGTR in AAB21130. (PubMed:1732746)Curated
    Sequence conflicti143 – 1431S → G in BAE25994. (PubMed:16141072)Curated
    Sequence conflicti558 – 5581K → N in AAB21130. (PubMed:1732746)Curated
    Sequence conflicti568 – 5681M → I in AAB21130. (PubMed:1732746)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 105105Missing in isoform 2 and isoform 3. 1 PublicationVSP_022143Add
    BLAST
    Alternative sequencei260 – 29435Missing in isoform 3 and isoform 4. 1 PublicationVSP_022144Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK089762 mRNA. Translation: BAC40955.1.
    AK139756 mRNA. Translation: BAE24128.1.
    AK144657 mRNA. Translation: BAE25994.1.
    AK152221 mRNA. Translation: BAE31048.1.
    AL671995 Genomic DNA. Translation: CAM13597.1.
    AL671995 Genomic DNA. Translation: CAM13598.1.
    BC056358 mRNA. Translation: AAH56358.1.
    BC063047 mRNA. Translation: AAH63047.1.
    S76673 mRNA. Translation: AAB21130.1.
    CCDSiCCDS52984.1. [Q64092-1]
    CCDS52985.1. [Q64092-4]
    CCDS72332.1. [Q64092-2]
    CCDS72333.1. [Q64092-3]
    PIRiA42029.
    RefSeqiNP_001098666.1. NM_001105196.1.
    NP_001098667.1. NM_001105197.1. [Q64092-2]
    NP_001258418.1. NM_001271489.1. [Q64092-2]
    NP_001258419.1. NM_001271490.1. [Q64092-2]
    NP_001258420.1. NM_001271491.1. [Q64092-3]
    NP_766060.2. NM_172472.3.
    XP_006527641.1. XM_006527578.1. [Q64092-2]
    UniGeneiMm.249142.

    Genome annotation databases

    EnsembliENSMUST00000101695; ENSMUSP00000099219; ENSMUSG00000000134. [Q64092-2]
    ENSMUST00000115677; ENSMUSP00000111341; ENSMUSG00000000134. [Q64092-2]
    ENSMUST00000115678; ENSMUSP00000111342; ENSMUSG00000000134. [Q64092-3]
    ENSMUST00000115679; ENSMUSP00000111343; ENSMUSG00000000134. [Q64092-2]
    ENSMUST00000137467; ENSMUSP00000134125; ENSMUSG00000000134. [Q64092-2]
    GeneIDi209446.
    KEGGimmu:209446.
    UCSCiuc009smg.2. mouse. [Q64092-1]
    uc009smk.2. mouse. [Q64092-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK089762 mRNA. Translation: BAC40955.1 .
    AK139756 mRNA. Translation: BAE24128.1 .
    AK144657 mRNA. Translation: BAE25994.1 .
    AK152221 mRNA. Translation: BAE31048.1 .
    AL671995 Genomic DNA. Translation: CAM13597.1 .
    AL671995 Genomic DNA. Translation: CAM13598.1 .
    BC056358 mRNA. Translation: AAH56358.1 .
    BC063047 mRNA. Translation: AAH63047.1 .
    S76673 mRNA. Translation: AAB21130.1 .
    CCDSi CCDS52984.1. [Q64092-1 ]
    CCDS52985.1. [Q64092-4 ]
    CCDS72332.1. [Q64092-2 ]
    CCDS72333.1. [Q64092-3 ]
    PIRi A42029.
    RefSeqi NP_001098666.1. NM_001105196.1.
    NP_001098667.1. NM_001105197.1. [Q64092-2 ]
    NP_001258418.1. NM_001271489.1. [Q64092-2 ]
    NP_001258419.1. NM_001271490.1. [Q64092-2 ]
    NP_001258420.1. NM_001271491.1. [Q64092-3 ]
    NP_766060.2. NM_172472.3.
    XP_006527641.1. XM_006527578.1. [Q64092-2 ]
    UniGenei Mm.249142.

    3D structure databases

    ProteinModelPortali Q64092.
    SMRi Q64092. Positions 346-436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229078. 1 interaction.
    IntActi Q64092. 1 interaction.

    PTM databases

    PhosphoSitei Q64092.

    Proteomic databases

    PaxDbi Q64092.
    PRIDEi Q64092.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000101695 ; ENSMUSP00000099219 ; ENSMUSG00000000134 . [Q64092-2 ]
    ENSMUST00000115677 ; ENSMUSP00000111341 ; ENSMUSG00000000134 . [Q64092-2 ]
    ENSMUST00000115678 ; ENSMUSP00000111342 ; ENSMUSG00000000134 . [Q64092-3 ]
    ENSMUST00000115679 ; ENSMUSP00000111343 ; ENSMUSG00000000134 . [Q64092-2 ]
    ENSMUST00000137467 ; ENSMUSP00000134125 ; ENSMUSG00000000134 . [Q64092-2 ]
    GeneIDi 209446.
    KEGGi mmu:209446.
    UCSCi uc009smg.2. mouse. [Q64092-1 ]
    uc009smk.2. mouse. [Q64092-3 ]

    Organism-specific databases

    CTDi 7030.
    MGIi MGI:98511. Tfe3.

    Phylogenomic databases

    eggNOGi NOG251286.
    GeneTreei ENSGT00390000004402.
    HOVERGENi HBG006768.
    InParanoidi A2AEW3.
    KOi K09105.

    Miscellaneous databases

    NextBioi 372667.
    PROi Q64092.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q64092.
    CleanExi MM_TCFE3.
    Genevestigatori Q64092.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR021802. bHLH_ZIP_TF_MiT/TFE.
    IPR024100. TFE3.
    [Graphical view ]
    PANTHERi PTHR10014:SF37. PTHR10014:SF37. 1 hit.
    Pfami PF11851. DUF3371. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Egg, Lung and Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    4. "mTFE3, an X-linked transcriptional activator containing basic helix-loop-helix and zipper domains, utilizes the zipper to stabilize both DNA binding and multimerization."
      Roman C., Matera A.G., Cooper C., Artandi S., Blain S., Ward D.C., Calame K.
      Mol. Cell. Biol. 12:817-827(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-572.
    5. "Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity."
      Huan C., Kelly M.L., Steele R., Shapira I., Gottesman S.R.S., Roman C.A.J.
      Nat. Immunol. 7:1082-1091(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTFE3_MOUSE
    AccessioniPrimary (citable) accession number: Q64092
    Secondary accession number(s): A2AEW3
    , A2AEW4, Q3U8H0, Q3UMV4, Q3UT52, Q7TNC1, Q8BN29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3