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Q64028

- PHC1_MOUSE

UniProt

Q64028 - PHC1_MOUSE

Protein

Polyhomeotic-like protein 1

Gene

Phc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (16 May 2003)
      Previous versions | rss
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    Functioni

    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri799 – 83335FCS-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to retinoic acid Source: MGI
    2. histone ubiquitination Source: UniProtKB
    3. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyhomeotic-like protein 1
    Short name:
    mPH1
    Alternative name(s):
    Early development regulatory protein 1
    RAE-28
    Gene namesi
    Name:Phc1
    Synonyms:Edr, Edr1, Rae28
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:103248. Phc1.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear body Source: MGI
    2. nucleus Source: MGI
    3. PRC1 complex Source: UniProtKB
    4. sex chromatin Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10121012Polyhomeotic-like protein 1PRO_0000058376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei653 – 6531PhosphoserineBy similarity
    Modified residuei906 – 9061PhosphoserineBy similarity
    Modified residuei930 – 9301PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ64028.

    PTM databases

    PhosphoSiteiQ64028.

    Expressioni

    Tissue specificityi

    Highly expressed in testis with lower levels in most other tissues.

    Developmental stagei

    Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in pharyngeal arches and weakly expressed in heart. By 14.5 dpc, expression is detected throughout the central nervous system.1 Publication

    Inductioni

    By retinoic acid.1 Publication

    Gene expression databases

    CleanExiMM_PHC1.
    GenevestigatoriQ64028.

    Interactioni

    Subunit structurei

    Homodimer. Component of a PRC1-like complex By similarity. Interacts with the SAM domain of SCMH1 via its SAM domain in vitro.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Mapkapk2P491382EBI-927346,EBI-298776
    Pcgf2P237984EBI-927346,EBI-926857
    Phc2Q9QWH12EBI-927346,EBI-642357
    Rnf2Q9CQJ43EBI-927346,EBI-927321
    Scmh1Q8K2142EBI-927346,EBI-445955

    Protein-protein interaction databases

    BioGridi199382. 13 interactions.
    DIPiDIP-456N.
    IntActiQ64028. 9 interactions.
    MINTiMINT-1172454.
    STRINGi10090.ENSMUSP00000078514.

    Structurei

    3D structure databases

    ProteinModelPortaliQ64028.
    SMRiQ64028. Positions 788-836, 946-1006.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini948 – 101265SAMPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi425 – 44824Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FCS-type zinc finger.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri799 – 83335FCS-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG145736.
    HOGENOMiHOG000115526.
    HOVERGENiHBG039325.
    InParanoidiQ64028.
    KOiK11456.
    PhylomeDBiQ64028.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    InterProiIPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    IPR012313. Znf_FCS.
    IPR010507. Znf_MYM.
    [Graphical view]
    PfamiPF00536. SAM_1. 1 hit.
    PF06467. zf-FCS. 1 hit.
    [Graphical view]
    SMARTiSM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    PROSITEiPS50105. SAM_DOMAIN. 1 hit.
    PS51024. ZF_FCS. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q64028-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    METESEQNSS STNGSSSSGA SSRPQIAQMS LYERQAVQAL QALQRQPNAA     50
    QYFHQFMLQQ QLSNAQLHSL AAVQQATIAA SRQASSPNSS TAQQQTATTQ 100
    ASMNLATTSA AQLISRSQSV SSPSATTLTQ SVLLGNTTSP PLNQSQAQMY 150
    LRPQLGNLLQ VNRTLGRNVP LASQLILMPN GAVAAVQQEV PPAQSPGVHA 200
    DADQVQNLAV RNQQASAQGP QMPGSTQKAI PPGASPVSGL SQTSSQALAV 250
    AQASSGASGQ SLNLSQAGGG SGNSLPGSMG PGGGGQAPGG LGQLPSSGLT 300
    GGSCPRKGTG VVQPLPAAQT VTVSQGSQTE AESAAAKKAE ADGSGQQSVG 350
    MNLTRTATPA PSQTLISSAT YTQIQPHSLI QQQQQIHLQQ KQVVIQQQIA 400
    IHHQQQFQHR QSQLLHTATH LQLAQQQQQQ QQQQQQQQQQ QQQQQQQQGT 450
    TLTAPQPPQV PPTQQVPPSQ SQQQAQTLVV QPMLQSSPLT LPPEPTSKPP 500
    IPIQSKPPVA PIKPPQLGAA KMSATQQPPP HIPVQVVGTR QPGSAQAQAL 550
    GLAQLAAAVP TPRGITGAVQ PGQAHLASSP PSSQAAPGAL QECPPALAAG 600
    MTLAPVQGTA HVVKGGPTAS SPVVAQVPAA FYMQSVHLPG KAQTLAVKRK 650
    AESEEERDDL SALASVLPTK ASPAAESPKV IEEKNSLGEK AEPVASLNAN 700
    PPNSDLVALA PTPSAPPPTL ALVSRQMGDS KPPQAIVKPQ ILTHIIEGFV 750
    IQEGAEPFPV GCSQFLKETE KPLQAGLPTG LNESQPSGPL GGDSPSVELE 800
    KKANLLKCEY CGKYAPAEQF RGSKRFCSMT CAKRYNVSCS HQFRLKRKKM 850
    KEFQEASYAR VRRRGPRRSS SDIARAKIQG KRHRGQEDSS RGSDNSSYDE 900
    ALSPTSPGPL SVRAGHGERD LGNTITTPST PELQGINPVF LSSNPSQWSV 950
    EEVYEFIASL QGCQEIAEEF RSQEIDGQAL LLLKEEHLMS AMNIKLGPAL 1000
    KICAKINVLK ET 1012
    Length:1,012
    Mass (Da):106,317
    Last modified:May 16, 2003 - v2
    Checksum:i5DA12A87D0B5FDDB
    GO
    Isoform 2 (identifier: Q64028-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         153-159: Missing.

    Show »
    Length:1,005
    Mass (Da):105,581
    Checksum:iD116BF86CE84AC8B
    GO
    Isoform 3 (identifier: Q64028-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         153-204: Missing.

    Show »
    Length:960
    Mass (Da):100,940
    Checksum:i31F04528026DECB9
    GO
    Isoform 4 (identifier: Q64028-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         76-76: A → V
         77-1012: Missing.

    Show »
    Length:76
    Mass (Da):8,354
    Checksum:i150ED0E73370CF43
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti127 – 1271T → A in AAC28974. (PubMed:9009205)Curated
    Sequence conflicti131 – 1311S → W in AAC28974. (PubMed:9009205)Curated
    Sequence conflicti401 – 4011I → Y in AAB31766. (PubMed:8070621)Curated
    Sequence conflicti425 – 4262Missing in BAC29400. (PubMed:16141072)Curated
    Sequence conflicti770 – 7701E → K in BAC29400. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei76 – 761A → V in isoform 4. 1 PublicationVSP_004038
    Alternative sequencei77 – 1012936Missing in isoform 4. 1 PublicationVSP_004039Add
    BLAST
    Alternative sequencei153 – 20452Missing in isoform 3. 1 PublicationVSP_004041Add
    BLAST
    Alternative sequencei153 – 1597Missing in isoform 2. 1 PublicationVSP_004040

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73882 mRNA. Translation: AAB31766.1.
    S73883 mRNA. No translation available.
    S73884 mRNA. No translation available.
    U63386 mRNA. Translation: AAC28974.1.
    BC046535 mRNA. Translation: AAH46535.1.
    AK036370 mRNA. Translation: BAC29400.1.
    RefSeqiNP_001258508.1. NM_001271579.1.
    UniGeneiMm.6822.

    Genome annotation databases

    GeneIDi13619.
    KEGGimmu:13619.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73882 mRNA. Translation: AAB31766.1 .
    S73883 mRNA. No translation available.
    S73884 mRNA. No translation available.
    U63386 mRNA. Translation: AAC28974.1 .
    BC046535 mRNA. Translation: AAH46535.1 .
    AK036370 mRNA. Translation: BAC29400.1 .
    RefSeqi NP_001258508.1. NM_001271579.1.
    UniGenei Mm.6822.

    3D structure databases

    ProteinModelPortali Q64028.
    SMRi Q64028. Positions 788-836, 946-1006.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199382. 13 interactions.
    DIPi DIP-456N.
    IntActi Q64028. 9 interactions.
    MINTi MINT-1172454.
    STRINGi 10090.ENSMUSP00000078514.

    PTM databases

    PhosphoSitei Q64028.

    Proteomic databases

    PRIDEi Q64028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13619.
    KEGGi mmu:13619.

    Organism-specific databases

    CTDi 1911.
    MGIi MGI:103248. Phc1.

    Phylogenomic databases

    eggNOGi NOG145736.
    HOGENOMi HOG000115526.
    HOVERGENi HBG039325.
    InParanoidi Q64028.
    KOi K11456.
    PhylomeDBi Q64028.

    Miscellaneous databases

    NextBioi 284292.
    PROi Q64028.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PHC1.
    Genevestigatori Q64028.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    InterProi IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR021129. SAM_type1.
    IPR012313. Znf_FCS.
    IPR010507. Znf_MYM.
    [Graphical view ]
    Pfami PF00536. SAM_1. 1 hit.
    PF06467. zf-FCS. 1 hit.
    [Graphical view ]
    SMARTi SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    PROSITEi PS50105. SAM_DOMAIN. 1 hit.
    PS51024. ZF_FCS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: one of the early inducible clones encodes a novel protein sharing several highly homologous regions with a Drosophila polyhomeotic protein."
      Nomura M., Takihara Y., Shimada K.
      Differentiation 57:39-50(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INDUCTION.
      Strain: BALB/c.
    2. "Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex."
      Alkema M.J., Bronk M., Verhoeven E., Otte A., van't Veer L.J., Berns A., van Lohuizen M.
      Genes Dev. 11:226-240(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), INTERACTION WITH BMI1.
      Tissue: Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: NMRI.
      Tissue: Mammary gland.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-845 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    5. "Targeted disruption of the mouse homologue of the Drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects."
      Takihara Y., Tomotsune D., Shirai M., Katoh-Fukui Y., Nishii K., Motaleb M.A., Nomura M., Tsuchiya R., Fujita Y., Shibata Y., Higashinakagawa T., Shimada K.
      Development 124:3673-3682(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A novel member of murine polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro."
      Tomotsune D., Takihara Y., Berger J., Duhl D., Joo S., Kyba M., Shirai M., Ohta H., Matsuda Y., Honda B.M., Simon J., Shimada K., Brock H.W., Randazzo F.
      Differentiation 65:229-239(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, INTERACTION WITH SCMH1.
    7. "Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes."
      Isono K., Fujimura Y., Shinga J., Yamaki M., O-Wang J., Takihara Y., Murahashi Y., Takada Y., Mizutani-Koseki Y., Koseki H.
      Mol. Cell. Biol. 25:6694-6706(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PHC2.

    Entry informationi

    Entry nameiPHC1_MOUSE
    AccessioniPrimary (citable) accession number: Q64028
    Secondary accession number(s): P70359, Q64307, Q8BZ80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: May 16, 2003
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3