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Q64028

- PHC1_MOUSE

UniProt

Q64028 - PHC1_MOUSE

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Protein

Polyhomeotic-like protein 1

Gene

Phc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri799 – 83335FCS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to retinoic acid Source: MGI
  2. histone ubiquitination Source: UniProtKB
  3. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Polyhomeotic-like protein 1
Short name:
mPH1
Alternative name(s):
Early development regulatory protein 1
RAE-28
Gene namesi
Name:Phc1
Synonyms:Edr, Edr1, Rae28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:103248. Phc1.

Subcellular locationi

GO - Cellular componenti

  1. nuclear body Source: MGI
  2. nucleus Source: MGI
  3. PRC1 complex Source: UniProtKB
  4. sex chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10121012Polyhomeotic-like protein 1PRO_0000058376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei653 – 6531PhosphoserineBy similarity
Modified residuei906 – 9061PhosphoserineBy similarity
Modified residuei930 – 9301PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ64028.
PRIDEiQ64028.

PTM databases

PhosphoSiteiQ64028.

Expressioni

Tissue specificityi

Highly expressed in testis with lower levels in most other tissues.

Developmental stagei

Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in pharyngeal arches and weakly expressed in heart. By 14.5 dpc, expression is detected throughout the central nervous system.1 Publication

Inductioni

By retinoic acid.1 Publication

Gene expression databases

CleanExiMM_PHC1.
GenevestigatoriQ64028.

Interactioni

Subunit structurei

Homodimer. Component of a PRC1-like complex (By similarity). Interacts with the SAM domain of SCMH1 via its SAM domain in vitro.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapkapk2P491382EBI-927346,EBI-298776
Pcgf2P237984EBI-927346,EBI-926857
Phc2Q9QWH12EBI-927346,EBI-642357
Rnf2Q9CQJ43EBI-927346,EBI-927321
Scmh1Q8K2142EBI-927346,EBI-445955

Protein-protein interaction databases

BioGridi199382. 13 interactions.
DIPiDIP-456N.
IntActiQ64028. 9 interactions.
MINTiMINT-1172454.
STRINGi10090.ENSMUSP00000078514.

Structurei

3D structure databases

ProteinModelPortaliQ64028.
SMRiQ64028. Positions 788-836, 946-1006.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini948 – 101265SAMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi425 – 44824Poly-GlnAdd
BLAST

Sequence similaritiesi

Contains 1 FCS-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri799 – 83335FCS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG145736.
HOGENOMiHOG000115526.
HOVERGENiHBG039325.
InParanoidiQ64028.
KOiK11456.
PhylomeDBiQ64028.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
IPR010507. Znf_MYM.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
PF06467. zf-FCS. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q64028-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METESEQNSS STNGSSSSGA SSRPQIAQMS LYERQAVQAL QALQRQPNAA
60 70 80 90 100
QYFHQFMLQQ QLSNAQLHSL AAVQQATIAA SRQASSPNSS TAQQQTATTQ
110 120 130 140 150
ASMNLATTSA AQLISRSQSV SSPSATTLTQ SVLLGNTTSP PLNQSQAQMY
160 170 180 190 200
LRPQLGNLLQ VNRTLGRNVP LASQLILMPN GAVAAVQQEV PPAQSPGVHA
210 220 230 240 250
DADQVQNLAV RNQQASAQGP QMPGSTQKAI PPGASPVSGL SQTSSQALAV
260 270 280 290 300
AQASSGASGQ SLNLSQAGGG SGNSLPGSMG PGGGGQAPGG LGQLPSSGLT
310 320 330 340 350
GGSCPRKGTG VVQPLPAAQT VTVSQGSQTE AESAAAKKAE ADGSGQQSVG
360 370 380 390 400
MNLTRTATPA PSQTLISSAT YTQIQPHSLI QQQQQIHLQQ KQVVIQQQIA
410 420 430 440 450
IHHQQQFQHR QSQLLHTATH LQLAQQQQQQ QQQQQQQQQQ QQQQQQQQGT
460 470 480 490 500
TLTAPQPPQV PPTQQVPPSQ SQQQAQTLVV QPMLQSSPLT LPPEPTSKPP
510 520 530 540 550
IPIQSKPPVA PIKPPQLGAA KMSATQQPPP HIPVQVVGTR QPGSAQAQAL
560 570 580 590 600
GLAQLAAAVP TPRGITGAVQ PGQAHLASSP PSSQAAPGAL QECPPALAAG
610 620 630 640 650
MTLAPVQGTA HVVKGGPTAS SPVVAQVPAA FYMQSVHLPG KAQTLAVKRK
660 670 680 690 700
AESEEERDDL SALASVLPTK ASPAAESPKV IEEKNSLGEK AEPVASLNAN
710 720 730 740 750
PPNSDLVALA PTPSAPPPTL ALVSRQMGDS KPPQAIVKPQ ILTHIIEGFV
760 770 780 790 800
IQEGAEPFPV GCSQFLKETE KPLQAGLPTG LNESQPSGPL GGDSPSVELE
810 820 830 840 850
KKANLLKCEY CGKYAPAEQF RGSKRFCSMT CAKRYNVSCS HQFRLKRKKM
860 870 880 890 900
KEFQEASYAR VRRRGPRRSS SDIARAKIQG KRHRGQEDSS RGSDNSSYDE
910 920 930 940 950
ALSPTSPGPL SVRAGHGERD LGNTITTPST PELQGINPVF LSSNPSQWSV
960 970 980 990 1000
EEVYEFIASL QGCQEIAEEF RSQEIDGQAL LLLKEEHLMS AMNIKLGPAL
1010
KICAKINVLK ET
Length:1,012
Mass (Da):106,317
Last modified:May 16, 2003 - v2
Checksum:i5DA12A87D0B5FDDB
GO
Isoform 2 (identifier: Q64028-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-159: Missing.

Show »
Length:1,005
Mass (Da):105,581
Checksum:iD116BF86CE84AC8B
GO
Isoform 3 (identifier: Q64028-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-204: Missing.

Show »
Length:960
Mass (Da):100,940
Checksum:i31F04528026DECB9
GO
Isoform 4 (identifier: Q64028-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-76: A → V
     77-1012: Missing.

Show »
Length:76
Mass (Da):8,354
Checksum:i150ED0E73370CF43
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271T → A in AAC28974. (PubMed:9009205)Curated
Sequence conflicti131 – 1311S → W in AAC28974. (PubMed:9009205)Curated
Sequence conflicti401 – 4011I → Y in AAB31766. (PubMed:8070621)Curated
Sequence conflicti425 – 4262Missing in BAC29400. (PubMed:16141072)Curated
Sequence conflicti770 – 7701E → K in BAC29400. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei76 – 761A → V in isoform 4. 1 PublicationVSP_004038
Alternative sequencei77 – 1012936Missing in isoform 4. 1 PublicationVSP_004039Add
BLAST
Alternative sequencei153 – 20452Missing in isoform 3. 1 PublicationVSP_004041Add
BLAST
Alternative sequencei153 – 1597Missing in isoform 2. 1 PublicationVSP_004040

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S73882 mRNA. Translation: AAB31766.1.
S73883 mRNA. No translation available.
S73884 mRNA. No translation available.
U63386 mRNA. Translation: AAC28974.1.
BC046535 mRNA. Translation: AAH46535.1.
AK036370 mRNA. Translation: BAC29400.1.
RefSeqiNP_001258508.1. NM_001271579.1.
UniGeneiMm.6822.

Genome annotation databases

GeneIDi13619.
KEGGimmu:13619.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S73882 mRNA. Translation: AAB31766.1 .
S73883 mRNA. No translation available.
S73884 mRNA. No translation available.
U63386 mRNA. Translation: AAC28974.1 .
BC046535 mRNA. Translation: AAH46535.1 .
AK036370 mRNA. Translation: BAC29400.1 .
RefSeqi NP_001258508.1. NM_001271579.1.
UniGenei Mm.6822.

3D structure databases

ProteinModelPortali Q64028.
SMRi Q64028. Positions 788-836, 946-1006.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199382. 13 interactions.
DIPi DIP-456N.
IntActi Q64028. 9 interactions.
MINTi MINT-1172454.
STRINGi 10090.ENSMUSP00000078514.

PTM databases

PhosphoSitei Q64028.

Proteomic databases

MaxQBi Q64028.
PRIDEi Q64028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13619.
KEGGi mmu:13619.

Organism-specific databases

CTDi 1911.
MGIi MGI:103248. Phc1.

Phylogenomic databases

eggNOGi NOG145736.
HOGENOMi HOG000115526.
HOVERGENi HBG039325.
InParanoidi Q64028.
KOi K11456.
PhylomeDBi Q64028.

Miscellaneous databases

NextBioi 284292.
PROi Q64028.
SOURCEi Search...

Gene expression databases

CleanExi MM_PHC1.
Genevestigatori Q64028.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
InterProi IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
IPR010507. Znf_MYM.
[Graphical view ]
Pfami PF00536. SAM_1. 1 hit.
PF06467. zf-FCS. 1 hit.
[Graphical view ]
SMARTi SM00454. SAM. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
PROSITEi PS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: one of the early inducible clones encodes a novel protein sharing several highly homologous regions with a Drosophila polyhomeotic protein."
    Nomura M., Takihara Y., Shimada K.
    Differentiation 57:39-50(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INDUCTION.
    Strain: BALB/c.
  2. "Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex."
    Alkema M.J., Bronk M., Verhoeven E., Otte A., van't Veer L.J., Berns A., van Lohuizen M.
    Genes Dev. 11:226-240(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), INTERACTION WITH BMI1.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: NMRI.
    Tissue: Mammary gland.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-845 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  5. "Targeted disruption of the mouse homologue of the Drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects."
    Takihara Y., Tomotsune D., Shirai M., Katoh-Fukui Y., Nishii K., Motaleb M.A., Nomura M., Tsuchiya R., Fujita Y., Shibata Y., Higashinakagawa T., Shimada K.
    Development 124:3673-3682(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A novel member of murine polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro."
    Tomotsune D., Takihara Y., Berger J., Duhl D., Joo S., Kyba M., Shirai M., Ohta H., Matsuda Y., Honda B.M., Simon J., Shimada K., Brock H.W., Randazzo F.
    Differentiation 65:229-239(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, INTERACTION WITH SCMH1.
  7. "Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes."
    Isono K., Fujimura Y., Shinga J., Yamaki M., O-Wang J., Takihara Y., Murahashi Y., Takada Y., Mizutani-Koseki Y., Koseki H.
    Mol. Cell. Biol. 25:6694-6706(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHC2.

Entry informationi

Entry nameiPHC1_MOUSE
AccessioniPrimary (citable) accession number: Q64028
Secondary accession number(s): P70359, Q64307, Q8BZ80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 16, 2003
Last modified: October 29, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3