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Q64028 (PHC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyhomeotic-like protein 1

Short name=mPH1
Alternative name(s):
Early development regulatory protein 1
RAE-28
Gene names
Name:Phc1
Synonyms:Edr, Edr1, Rae28
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1012 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression By similarity. Ref.5

Subunit structure

Homodimer. Component of a PRC1-like complex By similarity. Interacts with the SAM domain of SCMH1 via its SAM domain in vitro. Ref.2 Ref.6 Ref.7

Subcellular location

Nucleus.

Tissue specificity

Highly expressed in testis with lower levels in most other tissues.

Developmental stage

Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in pharyngeal arches and weakly expressed in heart. By 14.5 dpc, expression is detected throughout the central nervous system. Ref.6

Induction

By retinoic acid. Ref.1

Sequence similarities

Contains 1 FCS-type zinc finger.

Contains 1 SAM (sterile alpha motif) domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q64028-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q64028-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-159: Missing.
Isoform 3 (identifier: Q64028-3)

The sequence of this isoform differs from the canonical sequence as follows:
     153-204: Missing.
Isoform 4 (identifier: Q64028-4)

The sequence of this isoform differs from the canonical sequence as follows:
     76-76: A → V
     77-1012: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10121012Polyhomeotic-like protein 1
PRO_0000058376

Regions

Domain948 – 101265SAM
Zinc finger799 – 83335FCS-type
Compositional bias425 – 44824Poly-Gln

Amino acid modifications

Modified residue6531Phosphoserine By similarity
Modified residue9061Phosphoserine By similarity
Modified residue9301Phosphothreonine By similarity

Natural variations

Alternative sequence761A → V in isoform 4.
VSP_004038
Alternative sequence77 – 1012936Missing in isoform 4.
VSP_004039
Alternative sequence153 – 20452Missing in isoform 3.
VSP_004041
Alternative sequence153 – 1597Missing in isoform 2.
VSP_004040

Experimental info

Sequence conflict1271T → A in AAC28974. Ref.2
Sequence conflict1311S → W in AAC28974. Ref.2
Sequence conflict4011I → Y in AAB31766. Ref.1
Sequence conflict425 – 4262Missing in BAC29400. Ref.4
Sequence conflict7701E → K in BAC29400. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 16, 2003. Version 2.
Checksum: 5DA12A87D0B5FDDB

FASTA1,012106,317
        10         20         30         40         50         60 
METESEQNSS STNGSSSSGA SSRPQIAQMS LYERQAVQAL QALQRQPNAA QYFHQFMLQQ 

        70         80         90        100        110        120 
QLSNAQLHSL AAVQQATIAA SRQASSPNSS TAQQQTATTQ ASMNLATTSA AQLISRSQSV 

       130        140        150        160        170        180 
SSPSATTLTQ SVLLGNTTSP PLNQSQAQMY LRPQLGNLLQ VNRTLGRNVP LASQLILMPN 

       190        200        210        220        230        240 
GAVAAVQQEV PPAQSPGVHA DADQVQNLAV RNQQASAQGP QMPGSTQKAI PPGASPVSGL 

       250        260        270        280        290        300 
SQTSSQALAV AQASSGASGQ SLNLSQAGGG SGNSLPGSMG PGGGGQAPGG LGQLPSSGLT 

       310        320        330        340        350        360 
GGSCPRKGTG VVQPLPAAQT VTVSQGSQTE AESAAAKKAE ADGSGQQSVG MNLTRTATPA 

       370        380        390        400        410        420 
PSQTLISSAT YTQIQPHSLI QQQQQIHLQQ KQVVIQQQIA IHHQQQFQHR QSQLLHTATH 

       430        440        450        460        470        480 
LQLAQQQQQQ QQQQQQQQQQ QQQQQQQQGT TLTAPQPPQV PPTQQVPPSQ SQQQAQTLVV 

       490        500        510        520        530        540 
QPMLQSSPLT LPPEPTSKPP IPIQSKPPVA PIKPPQLGAA KMSATQQPPP HIPVQVVGTR 

       550        560        570        580        590        600 
QPGSAQAQAL GLAQLAAAVP TPRGITGAVQ PGQAHLASSP PSSQAAPGAL QECPPALAAG 

       610        620        630        640        650        660 
MTLAPVQGTA HVVKGGPTAS SPVVAQVPAA FYMQSVHLPG KAQTLAVKRK AESEEERDDL 

       670        680        690        700        710        720 
SALASVLPTK ASPAAESPKV IEEKNSLGEK AEPVASLNAN PPNSDLVALA PTPSAPPPTL 

       730        740        750        760        770        780 
ALVSRQMGDS KPPQAIVKPQ ILTHIIEGFV IQEGAEPFPV GCSQFLKETE KPLQAGLPTG 

       790        800        810        820        830        840 
LNESQPSGPL GGDSPSVELE KKANLLKCEY CGKYAPAEQF RGSKRFCSMT CAKRYNVSCS 

       850        860        870        880        890        900 
HQFRLKRKKM KEFQEASYAR VRRRGPRRSS SDIARAKIQG KRHRGQEDSS RGSDNSSYDE 

       910        920        930        940        950        960 
ALSPTSPGPL SVRAGHGERD LGNTITTPST PELQGINPVF LSSNPSQWSV EEVYEFIASL 

       970        980        990       1000       1010 
QGCQEIAEEF RSQEIDGQAL LLLKEEHLMS AMNIKLGPAL KICAKINVLK ET 

« Hide

Isoform 2 [UniParc].

Checksum: D116BF86CE84AC8B
Show »

FASTA1,005105,581
Isoform 3 [UniParc].

Checksum: 31F04528026DECB9
Show »

FASTA960100,940
Isoform 4 [UniParc].

Checksum: 150ED0E73370CF43
Show »

FASTA768,354

References

« Hide 'large scale' references
[1]"Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: one of the early inducible clones encodes a novel protein sharing several highly homologous regions with a Drosophila polyhomeotic protein."
Nomura M., Takihara Y., Shimada K.
Differentiation 57:39-50(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INDUCTION.
Strain: BALB/c.
[2]"Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex."
Alkema M.J., Bronk M., Verhoeven E., Otte A., van't Veer L.J., Berns A., van Lohuizen M.
Genes Dev. 11:226-240(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), INTERACTION WITH BMI1.
Tissue: Embryo.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: NMRI.
Tissue: Mammary gland.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-845 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cerebellum.
[5]"Targeted disruption of the mouse homologue of the Drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects."
Takihara Y., Tomotsune D., Shirai M., Katoh-Fukui Y., Nishii K., Motaleb M.A., Nomura M., Tsuchiya R., Fujita Y., Shibata Y., Higashinakagawa T., Shimada K.
Development 124:3673-3682(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A novel member of murine polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro."
Tomotsune D., Takihara Y., Berger J., Duhl D., Joo S., Kyba M., Shirai M., Ohta H., Matsuda Y., Honda B.M., Simon J., Shimada K., Brock H.W., Randazzo F.
Differentiation 65:229-239(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, INTERACTION WITH SCMH1.
[7]"Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes."
Isono K., Fujimura Y., Shinga J., Yamaki M., O-Wang J., Takihara Y., Murahashi Y., Takada Y., Mizutani-Koseki Y., Koseki H.
Mol. Cell. Biol. 25:6694-6706(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PHC2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S73882 mRNA. Translation: AAB31766.1.
S73883 mRNA. No translation available.
S73884 mRNA. No translation available.
U63386 mRNA. Translation: AAC28974.1.
BC046535 mRNA. Translation: AAH46535.1.
AK036370 mRNA. Translation: BAC29400.1.
RefSeqNP_001258508.1. NM_001271579.1.
UniGeneMm.6822.

3D structure databases

ProteinModelPortalQ64028.
SMRQ64028. Positions 788-836, 946-1006.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199382. 13 interactions.
DIPDIP-456N.
IntActQ64028. 9 interactions.
MINTMINT-1172454.
STRING10090.ENSMUSP00000078514.

PTM databases

PhosphoSiteQ64028.

Proteomic databases

PRIDEQ64028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13619.
KEGGmmu:13619.

Organism-specific databases

CTD1911.
MGIMGI:103248. Phc1.

Phylogenomic databases

eggNOGNOG145736.
HOGENOMHOG000115526.
HOVERGENHBG039325.
InParanoidQ64028.
KOK11456.
PhylomeDBQ64028.

Gene expression databases

CleanExMM_PHC1.
GenevestigatorQ64028.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
InterProIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
IPR010507. Znf_MYM.
[Graphical view]
PfamPF00536. SAM_1. 1 hit.
PF06467. zf-FCS. 1 hit.
[Graphical view]
SMARTSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284292.
PROQ64028.
SOURCESearch...

Entry information

Entry namePHC1_MOUSE
AccessionPrimary (citable) accession number: Q64028
Secondary accession number(s): P70359, Q64307, Q8BZ80
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 16, 2003
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot