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Protein

Polyhomeotic-like protein 1

Gene

Phc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri799 – 833FCS-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi6.3.2.19. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyhomeotic-like protein 1
Short name:
mPH1
Alternative name(s):
Early development regulatory protein 1
RAE-28
Gene namesi
Name:Phc1
Synonyms:Edr, Edr1, Rae28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103248. Phc1.

Subcellular locationi

GO - Cellular componenti

  • nuclear body Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • PcG protein complex Source: MGI
  • PRC1 complex Source: UniProtKB
  • sex chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583761 – 1012Polyhomeotic-like protein 1Add BLAST1012

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei653PhosphoserineBy similarity1
Modified residuei906PhosphoserineBy similarity1
Modified residuei930PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64028.
PRIDEiQ64028.

PTM databases

iPTMnetiQ64028.
PhosphoSitePlusiQ64028.

Expressioni

Tissue specificityi

Highly expressed in testis with lower levels in most other tissues.

Developmental stagei

Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in pharyngeal arches and weakly expressed in heart. By 14.5 dpc, expression is detected throughout the central nervous system.1 Publication

Inductioni

By retinoic acid.1 Publication

Gene expression databases

CleanExiMM_PHC1.

Interactioni

Subunit structurei

Homodimer. Component of a PRC1-like complex (By similarity). Interacts with the SAM domain of SCMH1 via its SAM domain in vitro.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapkapk2P491382EBI-927346,EBI-298776
Pcgf2P237984EBI-927346,EBI-926857
Phc2Q9QWH12EBI-927346,EBI-642357
Rnf2Q9CQJ43EBI-927346,EBI-927321
Scmh1Q8K2142EBI-927346,EBI-445955

Protein-protein interaction databases

BioGridi199382. 13 interactors.
DIPiDIP-456N.
IntActiQ64028. 10 interactors.
MINTiMINT-1172454.
STRINGi10090.ENSMUSP00000078514.

Structurei

3D structure databases

ProteinModelPortaliQ64028.
SMRiQ64028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini948 – 1012SAMPROSITE-ProRule annotationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi425 – 448Poly-GlnAdd BLAST24

Sequence similaritiesi

Contains 1 FCS-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri799 – 833FCS-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGCD. Eukaryota.
ENOG4111UWW. LUCA.
HOGENOMiHOG000115526.
HOVERGENiHBG039325.
InParanoidiQ64028.
KOiK11456.
PhylomeDBiQ64028.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METESEQNSS STNGSSSSGA SSRPQIAQMS LYERQAVQAL QALQRQPNAA
60 70 80 90 100
QYFHQFMLQQ QLSNAQLHSL AAVQQATIAA SRQASSPNSS TAQQQTATTQ
110 120 130 140 150
ASMNLATTSA AQLISRSQSV SSPSATTLTQ SVLLGNTTSP PLNQSQAQMY
160 170 180 190 200
LRPQLGNLLQ VNRTLGRNVP LASQLILMPN GAVAAVQQEV PPAQSPGVHA
210 220 230 240 250
DADQVQNLAV RNQQASAQGP QMPGSTQKAI PPGASPVSGL SQTSSQALAV
260 270 280 290 300
AQASSGASGQ SLNLSQAGGG SGNSLPGSMG PGGGGQAPGG LGQLPSSGLT
310 320 330 340 350
GGSCPRKGTG VVQPLPAAQT VTVSQGSQTE AESAAAKKAE ADGSGQQSVG
360 370 380 390 400
MNLTRTATPA PSQTLISSAT YTQIQPHSLI QQQQQIHLQQ KQVVIQQQIA
410 420 430 440 450
IHHQQQFQHR QSQLLHTATH LQLAQQQQQQ QQQQQQQQQQ QQQQQQQQGT
460 470 480 490 500
TLTAPQPPQV PPTQQVPPSQ SQQQAQTLVV QPMLQSSPLT LPPEPTSKPP
510 520 530 540 550
IPIQSKPPVA PIKPPQLGAA KMSATQQPPP HIPVQVVGTR QPGSAQAQAL
560 570 580 590 600
GLAQLAAAVP TPRGITGAVQ PGQAHLASSP PSSQAAPGAL QECPPALAAG
610 620 630 640 650
MTLAPVQGTA HVVKGGPTAS SPVVAQVPAA FYMQSVHLPG KAQTLAVKRK
660 670 680 690 700
AESEEERDDL SALASVLPTK ASPAAESPKV IEEKNSLGEK AEPVASLNAN
710 720 730 740 750
PPNSDLVALA PTPSAPPPTL ALVSRQMGDS KPPQAIVKPQ ILTHIIEGFV
760 770 780 790 800
IQEGAEPFPV GCSQFLKETE KPLQAGLPTG LNESQPSGPL GGDSPSVELE
810 820 830 840 850
KKANLLKCEY CGKYAPAEQF RGSKRFCSMT CAKRYNVSCS HQFRLKRKKM
860 870 880 890 900
KEFQEASYAR VRRRGPRRSS SDIARAKIQG KRHRGQEDSS RGSDNSSYDE
910 920 930 940 950
ALSPTSPGPL SVRAGHGERD LGNTITTPST PELQGINPVF LSSNPSQWSV
960 970 980 990 1000
EEVYEFIASL QGCQEIAEEF RSQEIDGQAL LLLKEEHLMS AMNIKLGPAL
1010
KICAKINVLK ET
Length:1,012
Mass (Da):106,317
Last modified:May 16, 2003 - v2
Checksum:i5DA12A87D0B5FDDB
GO
Isoform 2 (identifier: Q64028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-159: Missing.

Show »
Length:1,005
Mass (Da):105,581
Checksum:iD116BF86CE84AC8B
GO
Isoform 3 (identifier: Q64028-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-204: Missing.

Show »
Length:960
Mass (Da):100,940
Checksum:i31F04528026DECB9
GO
Isoform 4 (identifier: Q64028-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-76: A → V
     77-1012: Missing.

Show »
Length:76
Mass (Da):8,354
Checksum:i150ED0E73370CF43
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127T → A in AAC28974 (PubMed:9009205).Curated1
Sequence conflicti131S → W in AAC28974 (PubMed:9009205).Curated1
Sequence conflicti401I → Y in AAB31766 (PubMed:8070621).Curated1
Sequence conflicti425 – 426Missing in BAC29400 (PubMed:16141072).Curated2
Sequence conflicti770E → K in BAC29400 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00403876A → V in isoform 4. 1 Publication1
Alternative sequenceiVSP_00403977 – 1012Missing in isoform 4. 1 PublicationAdd BLAST936
Alternative sequenceiVSP_004041153 – 204Missing in isoform 3. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_004040153 – 159Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73882 mRNA. Translation: AAB31766.1.
S73883 mRNA. No translation available.
S73884 mRNA. No translation available.
U63386 mRNA. Translation: AAC28974.1.
BC046535 mRNA. Translation: AAH46535.1.
AK036370 mRNA. Translation: BAC29400.1.
RefSeqiNP_001258508.1. NM_001271579.1.
UniGeneiMm.6822.

Genome annotation databases

GeneIDi13619.
KEGGimmu:13619.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73882 mRNA. Translation: AAB31766.1.
S73883 mRNA. No translation available.
S73884 mRNA. No translation available.
U63386 mRNA. Translation: AAC28974.1.
BC046535 mRNA. Translation: AAH46535.1.
AK036370 mRNA. Translation: BAC29400.1.
RefSeqiNP_001258508.1. NM_001271579.1.
UniGeneiMm.6822.

3D structure databases

ProteinModelPortaliQ64028.
SMRiQ64028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199382. 13 interactors.
DIPiDIP-456N.
IntActiQ64028. 10 interactors.
MINTiMINT-1172454.
STRINGi10090.ENSMUSP00000078514.

PTM databases

iPTMnetiQ64028.
PhosphoSitePlusiQ64028.

Proteomic databases

PaxDbiQ64028.
PRIDEiQ64028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13619.
KEGGimmu:13619.

Organism-specific databases

CTDi1911.
MGIiMGI:103248. Phc1.

Phylogenomic databases

eggNOGiENOG410IGCD. Eukaryota.
ENOG4111UWW. LUCA.
HOGENOMiHOG000115526.
HOVERGENiHBG039325.
InParanoidiQ64028.
KOiK11456.
PhylomeDBiQ64028.

Enzyme and pathway databases

BRENDAi6.3.2.19. 3474.

Miscellaneous databases

ChiTaRSiPhc1. mouse.
PROiQ64028.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PHC1.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHC1_MOUSE
AccessioniPrimary (citable) accession number: Q64028
Secondary accession number(s): P70359, Q64307, Q8BZ80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 16, 2003
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.