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Protein

Glycine receptor subunit alpha-1

Gene

Glra1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

GO - Molecular functioni

GO - Biological processi

  • acrosome reaction Source: MGI
  • action potential Source: MGI
  • adult walking behavior Source: MGI
  • chloride transmembrane transport Source: MGI
  • chloride transport Source: UniProtKB
  • ion transport Source: UniProtKB
  • muscle contraction Source: UniProtKB
  • negative regulation of transmission of nerve impulse Source: UniProtKB
  • neuromuscular process Source: MGI
  • neuromuscular process controlling posture Source: MGI
  • neuropeptide signaling pathway Source: UniProtKB
  • positive regulation of acrosome reaction Source: MGI
  • regulation of inhibitory postsynaptic membrane potential Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of respiratory gaseous exchange Source: MGI
  • regulation of respiratory gaseous exchange by neurological system process Source: MGI
  • righting reflex Source: MGI
  • startle response Source: UniProtKB
  • synaptic transmission Source: MGI
  • synaptic transmission, glycinergic Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-1
Alternative name(s):
Glycine receptor 48 kDa subunit
Glycine receptor strychnine-binding subunit
Gene namesi
Name:Glra1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95747. Glra1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 247219ExtracellularCuratedAdd
BLAST
Transmembranei248 – 27427HelicalCuratedAdd
BLAST
Transmembranei281 – 29818HelicalCuratedAdd
BLAST
Transmembranei313 – 33624HelicalCuratedAdd
BLAST
Topological domaini337 – 42892CytoplasmicCuratedAdd
BLAST
Transmembranei429 – 44618HelicalCuratedAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • external side of plasma membrane Source: MGI
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • intracellular Source: MGI
  • intracellular membrane-bounded organelle Source: UniProtKB
  • membrane Source: MGI
  • postsynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Defects in Glra1 are the cause of the spasmodic (spd) phenotype, a mouse mutant which resembles to the human neurological disease, hyperekplexia (or startle disease (STHE)).

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828By similarityAdd
BLAST
Chaini29 – 457429Glycine receptor subunit alpha-1PRO_0000000413Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Curated
Disulfide bondi166 ↔ 180By similarity
Disulfide bondi226 ↔ 237By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ64018.
PRIDEiQ64018.

PTM databases

PhosphoSiteiQ64018.

Expressioni

Gene expression databases

BgeeiQ64018.
CleanExiMM_GLRA1.
ExpressionAtlasiQ64018. baseline and differential.
GenevestigatoriQ64018.

Interactioni

Subunit structurei

Pentamer composed of three alpha and two beta subunits. Homopentamers of alpha subunits also form functional receptors.

Structurei

3D structure databases

ProteinModelPortaliQ64018.
SMRiQ64018. Positions 42-340, 416-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2306Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ64018.
KOiK05193.
OMAiNTANPVP.
PhylomeDBiQ64018.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008128. Glycine_rcpt_A1.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01674. GLYRALPHA1.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q64018-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSFNTLRFY LWETIVFFSL AASKEAEAAR SAPKPMSPSD FLDKLMGRTS
60 70 80 90 100
GYDARIRPNF KGPPVNVSCN IFINSFGSIA ETTMDYRVNI FLRQQWNDPR
110 120 130 140 150
LAYNEYPDDS LDLDPSMLDS IWKPDLFFAN EKGAHFHEIT TDNKLLRISR
160 170 180 190 200
NGNVLYSIRI TLTLACPMDL KNFPMDVQTC IMQLESFGYT MNDLIFEWQE
210 220 230 240 250
QGAVQVADGL TLPQFILKEE KDLRYCTKHY NTGKFTCIEA RFHLERQMGY
260 270 280 290 300
YLIQMYIPSL LIVILSWISF WINMDAAPAR VGLGITTVLT MTTQSSGSRA
310 320 330 340 350
SLPKVSYVKA IDIWMAVCLL FVFSALLEYA AVNFVSRQHK ELLRFRRKRR
360 370 380 390 400
HHKSPMLNLF QDDEGGEGRF NFSAYGMGPA CLQAKDGISV KGANNNNTTN
410 420 430 440 450
PPPAPSKSPE EMRKLFIQRA KKIDKISRIG FPMAFLIFNM FYWIIYKIVR

REDVHNK
Length:457
Mass (Da):52,657
Last modified:December 8, 2000 - v2
Checksum:i29268DC4991A6E20
GO
Isoform b (identifier: Q64018-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     354-361: Missing.

Show »
Length:449
Mass (Da):51,726
Checksum:i4EDDAEB87874A153
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841M → I in CAB52398 (PubMed:7507926).Curated
Sequence conflicti84 – 841M → I in CAB52399 (PubMed:7507926).Curated
Sequence conflicti426 – 4294ISRI → NISH in CAB52398 (PubMed:7507926).Curated
Sequence conflicti426 – 4294ISRI → NISH in CAB52399 (PubMed:7507926).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti80 – 801A → S in spd. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei354 – 3618Missing in isoform b. 1 PublicationVSP_000080

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73717 mRNA. Translation: AAB32157.2.
S73718 mRNA. Translation: AAB32158.2.
X75832
, X75833, X75834, X75835, X75836, X75837, X75838, X75839, X75840 Genomic DNA. Translation: CAB52398.1.
X75832
, X75833, X75834, X75835, X75836, X75837, X75838, X75839, X75840 Genomic DNA. Translation: CAB52399.1.
AL596207 Genomic DNA. Translation: CAI35359.1.
CCDSiCCDS24715.1. [Q64018-2]
CCDS70190.1. [Q64018-1]
PIRiC49970.
RefSeqiNP_001277750.1. NM_001290821.1. [Q64018-1]
UniGeneiMm.89320.

Genome annotation databases

EnsembliENSMUST00000075603; ENSMUSP00000075032; ENSMUSG00000000263. [Q64018-1]
ENSMUST00000102716; ENSMUSP00000099777; ENSMUSG00000000263. [Q64018-2]
GeneIDi14654.
KEGGimmu:14654.
UCSCiuc007izo.1. mouse. [Q64018-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73717 mRNA. Translation: AAB32157.2.
S73718 mRNA. Translation: AAB32158.2.
X75832
, X75833, X75834, X75835, X75836, X75837, X75838, X75839, X75840 Genomic DNA. Translation: CAB52398.1.
X75832
, X75833, X75834, X75835, X75836, X75837, X75838, X75839, X75840 Genomic DNA. Translation: CAB52399.1.
AL596207 Genomic DNA. Translation: CAI35359.1.
CCDSiCCDS24715.1. [Q64018-2]
CCDS70190.1. [Q64018-1]
PIRiC49970.
RefSeqiNP_001277750.1. NM_001290821.1. [Q64018-1]
UniGeneiMm.89320.

3D structure databases

ProteinModelPortaliQ64018.
SMRiQ64018. Positions 42-340, 416-453.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi423.

PTM databases

PhosphoSiteiQ64018.

Proteomic databases

MaxQBiQ64018.
PRIDEiQ64018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075603; ENSMUSP00000075032; ENSMUSG00000000263. [Q64018-1]
ENSMUST00000102716; ENSMUSP00000099777; ENSMUSG00000000263. [Q64018-2]
GeneIDi14654.
KEGGimmu:14654.
UCSCiuc007izo.1. mouse. [Q64018-1]

Organism-specific databases

CTDi2741.
MGIiMGI:95747. Glra1.

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ64018.
KOiK05193.
OMAiNTANPVP.
PhylomeDBiQ64018.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.

Miscellaneous databases

PROiQ64018.
SOURCEiSearch...

Gene expression databases

BgeeiQ64018.
CleanExiMM_GLRA1.
ExpressionAtlasiQ64018. baseline and differential.
GenevestigatoriQ64018.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008128. Glycine_rcpt_A1.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01674. GLYRALPHA1.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A missense mutation in the gene encoding the alpha 1 subunit of the inhibitory glycine receptor in the spasmodic mouse."
    Ryan S.G., Buckwalter M.S., Lynch J.W., Handford C.A., Segura L., Shiang R., Wasmuth J.J., Camper S.A., Schofield P., O'Connell P.
    Nat. Genet. 7:131-135(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), VARIANT SPD SER-80.
  2. "Structural analysis of mouse glycine receptor alpha subunit genes. Identification and chromosomal localization of a novel variant."
    Matzenbach B., Maulet Y., Sefton L., Courtier B., Avner P., Guenet J.-L., Betz H.
    J. Biol. Chem. 269:2607-2612(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: BALB/c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiGLRA1_MOUSE
AccessioniPrimary (citable) accession number: Q64018
Secondary accession number(s): Q5NCT8
, Q64019, Q9R0Y6, Q9R0Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 8, 2000
Last modified: April 1, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.