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Protein

Ras-related protein Rab-34

Gene

Rab34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium. Plays a role in the fusion of phagosomes with lysosomes (By similarity). Involved in the redistribution of lysosomes to the peri-Golgi region.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 668GTPBy similarity
Nucleotide bindingi107 – 1115GTPBy similarity
Nucleotide bindingi166 – 1694GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • guanyl nucleotide binding Source: MGI
  • Ral GTPase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-34
Alternative name(s):
Ras-related homolog
Ras-related protein Rab-39
Ras-related protein Rah
Gene namesi
Name:Rab34
Synonyms:Rab39, Rah, Rah1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104606. Rab34.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Golgi apparatus 1 Publication
  • Cytoplasmic vesiclephagosome By similarity
  • Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

  • Note: Recruited to phagosomes containing S.aureus or Mycobacterium.By similarity

GO - Cellular componenti

  • early endosome Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi cisterna Source: MGI
  • Golgi stack Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • ruffle Source: MGI
  • vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi82 – 821K → Q: Abolishes interaction with RILP and localization to the peri-Golgi region. 1 Publication
Mutagenesisi82 – 821K → Y: Does not abolish interaction with RILP and localization to the peri-Golgi region. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Ras-related protein Rab-34PRO_0000121244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei241 – 2411PhosphoserineCombined sources
Lipidationi257 – 2571S-geranylgeranyl cysteineSequence analysis
Lipidationi258 – 2581S-geranylgeranyl cysteineSequence analysis

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ64008.
PaxDbiQ64008.
PRIDEiQ64008.

PTM databases

iPTMnetiQ64008.
PhosphoSiteiQ64008.
SwissPalmiQ64008.

Expressioni

Gene expression databases

CleanExiMM_RAB34.
MM_RAB39.

Interactioni

Subunit structurei

Interacts with RILP.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202576. 3 interactions.
IntActiQ64008. 7 interactions.
STRINGi10090.ENSMUSP00000002128.

Structurei

3D structure databases

ProteinModelPortaliQ64008.
SMRiQ64008. Positions 23-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 899Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
HOVERGENiHBG106693.
InParanoidiQ64008.
KOiK07921.
PhylomeDBiQ64008.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILAPVRRD RVLAELPQCL KKEAALHVRK DFHPRVTCAC QEHRTGTVGF
60 70 80 90 100
KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGVP
110 120 130 140 150
FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLTDA
160 170 180 190 200
LKENDPSNVL LFLVGSKKDL STPAQYSLME KDALKVAQEI KAEYWAVSSL
210 220 230 240 250
TGENVREFFF RVAALTFEAN VLADVEKSGA RHIADVVRIN SDDKNLYLTA

SKKKATCCP
Length:259
Mass (Da):29,101
Last modified:January 31, 2002 - v2
Checksum:i158F5132F0DDE808
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521I → N in AAB20669 (PubMed:1752434).Curated
Sequence conflicti224 – 2252DV → EL (Ref. 3) Curated
Sequence conflicti224 – 2252DV → EL (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322068 mRNA. Translation: AAK09398.1.
AF327929 mRNA. Translation: AAK11241.1.
AB082927 mRNA. Translation: BAC16803.1.
BC038638 mRNA. Translation: AAH38638.1.
S72304 mRNA. Translation: AAB20669.1.
CCDSiCCDS25094.1.
PIRiA41636.
RefSeqiNP_001152954.1. NM_001159482.1.
NP_258436.2. NM_033475.3.
UniGeneiMm.275864.

Genome annotation databases

GeneIDi19376.
KEGGimmu:19376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322068 mRNA. Translation: AAK09398.1.
AF327929 mRNA. Translation: AAK11241.1.
AB082927 mRNA. Translation: BAC16803.1.
BC038638 mRNA. Translation: AAH38638.1.
S72304 mRNA. Translation: AAB20669.1.
CCDSiCCDS25094.1.
PIRiA41636.
RefSeqiNP_001152954.1. NM_001159482.1.
NP_258436.2. NM_033475.3.
UniGeneiMm.275864.

3D structure databases

ProteinModelPortaliQ64008.
SMRiQ64008. Positions 23-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202576. 3 interactions.
IntActiQ64008. 7 interactions.
STRINGi10090.ENSMUSP00000002128.

PTM databases

iPTMnetiQ64008.
PhosphoSiteiQ64008.
SwissPalmiQ64008.

Proteomic databases

MaxQBiQ64008.
PaxDbiQ64008.
PRIDEiQ64008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19376.
KEGGimmu:19376.

Organism-specific databases

CTDi83871.
MGIiMGI:104606. Rab34.

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
HOVERGENiHBG106693.
InParanoidiQ64008.
KOiK07921.
PhylomeDBiQ64008.

Miscellaneous databases

NextBioi296471.
PROiQ64008.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RAB34.
MM_RAB39.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Rab39 coding region (cDNA)."
    Hong W.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Full length sequence of Rah, a novel member of the Rab family of small GTPases."
    Hari M., Morimoto B.H., Asai D.J.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neuron.
  3. "Rah, a Rab family small GTPase."
    Sun P., Yamamoto H., Endo T.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Lung.
  5. "Molecular cloning of a member of a new class of low-molecular-weight GTP-binding proteins."
    Morimoto B.H., Chuang C.-C., Koshland D.E. Jr.
    Genes Dev. 5:2386-2391(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 52-259.
    Tissue: Neuron.
  6. "Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein."
    Wang T., Hong W.
    Mol. Biol. Cell 13:4317-4332(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LYSOSOME REDISTRIBUTION, INTERACTION WITH RILP, MUTAGENESIS OF LYS-82, SUBCELLULAR LOCATION.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung and Testis.

Entry informationi

Entry nameiRAB34_MOUSE
AccessioniPrimary (citable) accession number: Q64008
Secondary accession number(s): Q8BHJ0, Q99P59, Q99P90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 31, 2002
Last modified: May 11, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.