Q63ZY3 (KANK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KN motif and ankyrin repeat domain-containing protein 2

Alternative name(s):
Ankyrin repeat domain-containing protein 25
Matrix-remodeling-associated protein 3
SRC-1-interacting protein
Short name=SRC1-interacting protein

Gene names

Name:	KANK2
Synonyms:	ANKRD25, KIAA1518, MXRA3, SIP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Tissue specificity	Strongly expressed in cervix, colon, heart, kidney and lung. Ref.2
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7 Ref.8 Ref.9 Ref.10
Sequence similarities	Contains 5 ANK repeats.
Sequence caution	The sequence BAA92081.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA96042.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14531.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q63ZY3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q63ZY3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     472-472: T → TAPPLSSPP

Note: No experimental confirmation available.

Isoform 3 (identifier: Q63ZY3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     835-841: FAPMSDD → VSLNPAG
     842-851: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	KN motif and ankyrin repeat domain-containing protein 2		PRO_0000240840
Regions
	Repeat	666 – 696	31	ANK 1
	Repeat	700 – 733	34	ANK 2
	Repeat	738 – 767	30	ANK 3
	Repeat	771 – 801	31	ANK 4
	Repeat	805 – 835	31	ANK 5
	Coiled coil	183 – 234	52	Potential
	Coiled coil	282 – 312	31	Potential
	Compositional bias	425 – 428	4	Poly-Ser
Amino acid modifications
	Modified residue	19	1	Phosphoserine Ref.7
	Modified residue	72	1	Phosphoserine Ref.9
	Modified residue	246	1	Phosphoserine By similarity
	Modified residue	356	1	Phosphoserine Ref.9
	Modified residue	375	1	Phosphoserine Ref.7 Ref.9 Ref.10
	Modified residue	463	1	Phosphoserine Ref.9
	Modified residue	472	1	Phosphothreonine Ref.9
	Modified residue	540	1	Phosphoserine Ref.7
	Modified residue	568	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	472	1	T → TAPPLSSPP in isoform 2.		VSP_019427
	Alternative sequence	835 – 841	7	FAPMSDD → VSLNPAG in isoform 3.		VSP_019428
	Alternative sequence	842 – 851	10	Missing in isoform 3.		VSP_019429
	Natural variant	118	1	G → S. Corresponds to variant rs755237 [ dbSNP | Ensembl ].		VAR_048304
	Natural variant	401	1	M → T. Corresponds to variant rs17616661 [ dbSNP | Ensembl ].		VAR_048305
Experimental info
	Sequence conflict	525	1	D → N in BAA96042. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: C14CD8B937A7B6E8	FASTA	851	91,174
	10 20 30 40 50 60 MAQVLHVPAP FPGTPGPASP PAFPAKDPDP PYSVETPYGY RLDLDFLKYV DDIEKGHTLR 70 80 90 100 110 120 RVAVQRRPRL SSLPRGPGSW WTSTESLCSN ASGDSRHSAY SYCGRGFYPQ YGALETRGGF 130 140 150 160 170 180 NPRVERTLLD ARRRLEDQAA TPTGLGSLTP SAAGSTASLV GVGLPPPTPR SSGLSTPVPP 190 200 210 220 230 240 SAGHLAHVRE QMAGALRKLR QLEEQVKLIP VLQVKLSVLQ EEKRQLTVQL KSQKFLGHPT 250 260 270 280 290 300 AGRGRSELCL DLPDPPEDPV ALETRSVGTW VRERDLGMPD GEAALAAKVA VLETQLKKAL 310 320 330 340 350 360 QELQAAQARQ ADPQPQAWPP PDSPVRVDTV RVVEGPREVE VVASTAAGAP AQRAQSLEPY 370 380 390 400 410 420 GTGLRALAMP GRPESPPVFR SQEVVETMCP VPAAATSNVH MVKKISITER SCDGAAGLPE 430 440 450 460 470 480 VPAESSSSPP GSEVASLTQP EKSTGRVPTQ EPTHREPTRQ AASQESEEAG GTGGPPAGVR 490 500 510 520 530 540 SIMKRKEEVA DPTAHRRSLQ FVGVNGGYES SSEDSSTAEN ISDNDSTENE APEPRERVPS 550 560 570 580 590 600 VAEAPQLRPA GTAAAKTSRQ ECQLSRESQH IPTAEGASGS NTEEEIRMEL SPDLISACLA 610 620 630 640 650 660 LEKYLDNPNA LTERELKVAY TTVLQEWLRL ACRSDAHPEL VRRHLVTFRA MSARLLDYVV 670 680 690 700 710 720 NIADSNGNTA LHYSVSHANF PVVQQLLDSG VCKVDKQNRA GYSPIMLTAL ATLKTQDDIE 730 740 750 760 770 780 TVLQLFRLGN INAKASQAGQ TALMLAVSHG RVDVVKALLA CEADVNVQDD DGSTALMCAC 790 800 810 820 830 840 EHGHKEIAGL LLAVPSCDIS LTDRDGSTAL MVALDAGQSE IASMLYSRMN IKCSFAPMSD 850 DESPTSSSAE E
	Isoform 2 [UniParc]. Checksum: 0629BE4E0E29FCEE	FASTA	859	91,921
	10 20 30 40 50 60 MAQVLHVPAP FPGTPGPASP PAFPAKDPDP PYSVETPYGY RLDLDFLKYV DDIEKGHTLR 70 80 90 100 110 120 RVAVQRRPRL SSLPRGPGSW WTSTESLCSN ASGDSRHSAY SYCGRGFYPQ YGALETRGGF 130 140 150 160 170 180 NPRVERTLLD ARRRLEDQAA TPTGLGSLTP SAAGSTASLV GVGLPPPTPR SSGLSTPVPP 190 200 210 220 230 240 SAGHLAHVRE QMAGALRKLR QLEEQVKLIP VLQVKLSVLQ EEKRQLTVQL KSQKFLGHPT 250 260 270 280 290 300 AGRGRSELCL DLPDPPEDPV ALETRSVGTW VRERDLGMPD GEAALAAKVA VLETQLKKAL 310 320 330 340 350 360 QELQAAQARQ ADPQPQAWPP PDSPVRVDTV RVVEGPREVE VVASTAAGAP AQRAQSLEPY 370 380 390 400 410 420 GTGLRALAMP GRPESPPVFR SQEVVETMCP VPAAATSNVH MVKKISITER SCDGAAGLPE 430 440 450 460 470 480 VPAESSSSPP GSEVASLTQP EKSTGRVPTQ EPTHREPTRQ AASQESEEAG GTAPPLSSPP 490 500 510 520 530 540 GGPPAGVRSI MKRKEEVADP TAHRRSLQFV GVNGGYESSS EDSSTAENIS DNDSTENEAP 550 560 570 580 590 600 EPRERVPSVA EAPQLRPAGT AAAKTSRQEC QLSRESQHIP TAEGASGSNT EEEIRMELSP 610 620 630 640 650 660 DLISACLALE KYLDNPNALT ERELKVAYTT VLQEWLRLAC RSDAHPELVR RHLVTFRAMS 670 680 690 700 710 720 ARLLDYVVNI ADSNGNTALH YSVSHANFPV VQQLLDSGVC KVDKQNRAGY SPIMLTALAT 730 740 750 760 770 780 LKTQDDIETV LQLFRLGNIN AKASQAGQTA LMLAVSHGRV DVVKALLACE ADVNVQDDDG 790 800 810 820 830 840 STALMCACEH GHKEIAGLLL AVPSCDISLT DRDGSTALMV ALDAGQSEIA SMLYSRMNIK 850 CSFAPMSDDE SPTSSSAEE
	Isoform 3 [UniParc]. Checksum: D82155CD26E514F1	FASTA	841	90,044
	10 20 30 40 50 60 MAQVLHVPAP FPGTPGPASP PAFPAKDPDP PYSVETPYGY RLDLDFLKYV DDIEKGHTLR 70 80 90 100 110 120 RVAVQRRPRL SSLPRGPGSW WTSTESLCSN ASGDSRHSAY SYCGRGFYPQ YGALETRGGF 130 140 150 160 170 180 NPRVERTLLD ARRRLEDQAA TPTGLGSLTP SAAGSTASLV GVGLPPPTPR SSGLSTPVPP 190 200 210 220 230 240 SAGHLAHVRE QMAGALRKLR QLEEQVKLIP VLQVKLSVLQ EEKRQLTVQL KSQKFLGHPT 250 260 270 280 290 300 AGRGRSELCL DLPDPPEDPV ALETRSVGTW VRERDLGMPD GEAALAAKVA VLETQLKKAL 310 320 330 340 350 360 QELQAAQARQ ADPQPQAWPP PDSPVRVDTV RVVEGPREVE VVASTAAGAP AQRAQSLEPY 370 380 390 400 410 420 GTGLRALAMP GRPESPPVFR SQEVVETMCP VPAAATSNVH MVKKISITER SCDGAAGLPE 430 440 450 460 470 480 VPAESSSSPP GSEVASLTQP EKSTGRVPTQ EPTHREPTRQ AASQESEEAG GTGGPPAGVR 490 500 510 520 530 540 SIMKRKEEVA DPTAHRRSLQ FVGVNGGYES SSEDSSTAEN ISDNDSTENE APEPRERVPS 550 560 570 580 590 600 VAEAPQLRPA GTAAAKTSRQ ECQLSRESQH IPTAEGASGS NTEEEIRMEL SPDLISACLA 610 620 630 640 650 660 LEKYLDNPNA LTERELKVAY TTVLQEWLRL ACRSDAHPEL VRRHLVTFRA MSARLLDYVV 670 680 690 700 710 720 NIADSNGNTA LHYSVSHANF PVVQQLLDSG VCKVDKQNRA GYSPIMLTAL ATLKTQDDIE 730 740 750 760 770 780 TVLQLFRLGN INAKASQAGQ TALMLAVSHG RVDVVKALLA CEADVNVQDD DGSTALMCAC 790 800 810 820 830 840 EHGHKEIAGL LLAVPSCDIS LTDRDGSTAL MVALDAGQSE IASMLYSRMN IKCSVSLNPA G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SIP, a novel SRC-1 interacting protein implicated in nuclear receptor signal pathway." Zhang Y., Zhang H., Shang Y. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Kank proteins: a new family of ankyrin-repeat domain-containing proteins." Zhu Y., Kakinuma N., Wang Y., Kiyama R. Biochim. Biophys. Acta 1780:128-133(2008) [PubMed: 17996375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix and Lung.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 386-841 (ISOFORM 3). Tissue: Ovary and Placenta.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-375 AND SER-540, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-356; SER-375; SER-463 AND THR-472, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY639929 mRNA. Translation: AAT57879.1. AB284125 mRNA. Translation: BAG06864.1. AB040951 mRNA. Translation: BAA96042.2. Different initiation. CH471106 Genomic DNA. Translation: EAW84177.1. BC082762 mRNA. Translation: AAH82762.1. BC098105 mRNA. Translation: AAH98105.1. BC098286 mRNA. Translation: AAH98286.1. BC098312 mRNA. Translation: AAH98312.1. BC105989 mRNA. Translation: AAI05990.1. AK002094 mRNA. Translation: BAA92081.1. Different initiation. AK023332 mRNA. Translation: BAB14531.1. Different initiation.
IPI	IPI00470559. IPI00760928. IPI00760962.
RefSeq	NP_001129663.1. NM_001136191.2. NP_056308.3. NM_015493.6.
UniGene	Hs.284208. Hs.723365. Hs.726146.
3D structure databases
ProteinModelPortal	Q63ZY3.
SMR	Q63ZY3. Positions 664-828.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q63ZY3. 5 interactions.
MINT	MINT-4540700.
STRING	Q63ZY3.
PTM databases
PhosphoSite	Q63ZY3.
Polymorphism databases
DMDM	74757262.
Proteomic databases
PRIDE	Q63ZY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355150; ENSP00000347276; ENSG00000197256. ENST00000432929; ENSP00000395650; ENSG00000197256.
GeneID	25959.
KEGG	hsa:25959.
UCSC	uc002mqm.1. human. uc002mqo.2. human. uc002mqp.1. human.
Organism-specific databases
CTD	25959.
GeneCards	GC19M011274.
H-InvDB	HIX0014762.
HGNC	HGNC:29300. KANK2.
HPA	HPA015643.
neXtProt	NX_Q63ZY3.
PharmGKB	PA134945449. PA162392581.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG062256.
InParanoid	Q63ZY3.
OMA	TWVRERD.
OrthoDB	EOG4HQDHX.
Gene expression databases
ArrayExpress	Q63ZY3.
Bgee	Q63ZY3.
CleanEx	HS_KANK2.
Genevestigator	Q63ZY3.
GermOnline	ENSG00000197256. Homo sapiens.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR021939. KN_motif. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF12796. Ank_2. 1 hit. PF12075. KN_motif. 1 hit. [Graphical view]
SMART	SM00248. ANK. 5 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	47574.

Entry information

Entry name

KANK2_HUMAN

Accession

Primary (citable) accession number: Q63ZY3
Secondary accession number(s): B0I1P4 , Q3KQZ3, Q6GUF5, Q9H8S4, Q9NUP0, Q9P210

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.