Q63Y16 (ACEK_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	BPSL0373

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057897
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63Y16 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: EB035EFF2E5EFB82	FASTA	603	69,894
	10 20 30 40 50 60 MNHFPKLLSS QIGFDVAQTI LENFDRHYRI FREAAVEAKD LFERADWHGL QRLARERITS 70 80 90 100 110 120 YDDRVRECVE LLEDEYDAEN IDNEVWPQIK LHYIGLLTSH RQPECAETFF NSVCCKILHR 130 140 150 160 170 180 AYFNNDFIFV RPAISTEYIE NDEPAAKPTY RAYYPGSEGL AATLERIVTN FQLNPPFEDL 190 200 210 220 230 240 ERDIACIMQA IHDEFGAFDE AVNFQIHVLS SLFYRNKTAY VVGRIINGDR VLPFAVPIRH 250 260 270 280 290 300 ARAGILALDT VLLRRDQLKI IFSFSHSYFL VDMNVPSAYV QFLRSIMPGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLHHLSHS SDRFIVAPGI KGLVMLVFTL PSFPYVFKMI KDHFPPPKDT 370 380 390 400 410 420 TREQIMAKYL LVKRHDRLGR MADTLEYSSV ALPLARLDDA LVRELEKEVP SLIEYEGENL 430 440 450 460 470 480 VIKHLYIERR MVPLNLYLQN GSDAEIEHGV REYGNAVKEL MQANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR RVPPPRNDED EMSGEPWYTV GPHDIFPETY APFLLGDPRV 550 560 570 580 590 600 REHFLAHHAD FFDPQLWQDS KDRLLRGELP DFFAYEPALR FCIRYPERFA PGDAADGGKL AAA

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH34361.1.
RefSeq	YP_106999.1. NC_006350.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3092419.
GenomeReviews	Gene locus BPSL0373 in contig BX571965_GR.
KEGG	bps:BPSL0373.
PATRIC	19260377. VBIBurPse99623_0420.
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL0373-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_BURPS

Accession

Primary (citable) accession number: Q63Y16

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program