Reviewed, UniProtKB/Swiss-Prot Q63XR8 (DNAE2_BURPS)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BPSL0472

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	1072 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1072	1072	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103374

Sequences

	Sequence		Length	Mass (Da)
	Q63XR8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: C627677138FFFF65	FASTA	1,072	118,134
	10 20 30 40 50 60 MDAASGILPD YAELFCRSNF SFLHGASSAE ELVERAAKQG YRGIAITDEC SLAGAPRMHV 70 80 90 100 110 120 AAKAVGLPLV VGAYFGVTPD DAAPGHDPGP GAFGLVLLAQ NREGYGNLSE LISWRRMNAP 130 140 150 160 170 180 KGTYRLTPRM LAAPPRALAH LRGVPDCFAI LVPTYPARAD VLDAQLAWFD ALFGERARLG 190 200 210 220 230 240 LVQLQRALDG AHREQVRAAG ERRGMHIVAL GDVTMHIRSC KPLQDTMTAI RLGMPIAECG 250 260 270 280 290 300 HALAPNGEQH LRTRQRIAQL FPADALAQTC RMLDACHFSL DDLRYEYPHE IVPAGHTPTS 310 320 330 340 350 360 YLAQETWAGA RRRYPDGVPD TVRQRIEFEL ALIADLKYEP YFLTVYDIVK YARSKDILCQ 370 380 390 400 410 420 GRGSAANSVV CYCLGVTEVN PQQSTLLFER FLSRERGEPP DIDVDFEHQR REEVIQYLYE 430 440 450 460 470 480 KYGHDRAALA AAVSTYRPRG ALRETGKALG VDPMLVERVA KEHRWFDGSR DLLARFASVG 490 500 510 520 530 540 LDPEVPLIRT WAEIAARLLN FPRHLSQHSG GFVVSRGKLT RLVPVENAAM EGRRVIQWDK 550 560 570 580 590 600 DDLEALGLMK VDVLALGMLS ALHRAFDMIT AWRGPPLPDG RPFRLEHIPQ DDEATYDMIC 610 620 630 640 650 660 RADTVGVFQI ESRAQMSMLP RLRPRGYYDL VVQVSIVRPG PIQGGAVHPY LERRRIAAGE 670 680 690 700 710 720 AHGEITYPSE ALERVLERTL GIPIFQEQVM QIAIVAAGFT PGEADALRRA MAAWKRKGDL 730 740 750 760 770 780 GKYHERIVAG MLERGYSREF AEQIFEQIKG FGEYGFPESH AASFAKLAYA SSWLKRHEPA 790 800 810 820 830 840 IFLAALLNSQ PMGFYPPAQL VQDAKRHGVT VLPIDATKSG WEASLEAQPG AAPPDGRPAV 850 860 870 880 890 900 RLGLSLVRGL GEEAARRIGA ARAAGPFASV DELARRACLE RRDLEALAAA NAFATLAGNR 910 920 930 940 950 960 RDALWQAVAA APERGLLAAA PIDEAVRPAL GAPTEADDVF ADYRTIGLTL NRHPVALLRP 970 980 990 1000 1010 1020 ALDARRLSSA AALRDRRNGR LARACGLVTA RQMPGTAKGV LFVTLEDETG CVNVIVRPEL 1030 1040 1050 1060 1070 LERQRRETLD SQLLAVSGVW QCESDVRHLV AQYLEDLTPL IAGLRTESRE FH

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH34461.1.
RefSeq	YP_107097.1.
3D structure databases
SMR	Q63XR8. Positions 9-908.
ModBase	Search...
Genome annotation databases
GeneID	3092552.
GenomeReviews	Gene locus BPSL0472 in contig BX571965_GR.
KEGG	bps:BPSL0472.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL0472-MONOMER.
BRENDA	2.7.7.7. 275934.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BURPS

Accession

Primary (citable) accession number: Q63XR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)