Reviewed, UniProtKB/Swiss-Prot Q63X54 (AROA1_BURPS)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BPSL0683

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm Probable HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088238

Sequences

	Sequence		Length	Mass (Da)
	Q63X54-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E1B61DE73CCC5233	FASTA	451	48,627
	10 20 30 40 50 60 MTTSDRLQPS FVEVKNTSTL SGTIDLPASK SSSTRALLTA ALTPGISTIR NVATGFNSNA 70 80 90 100 110 120 MKHNCERLGA SFSSEGDTTV VKGVDVMHVD REIVFDPGNS GVVLRLLMGV AGYLPDTRFV 130 140 150 160 170 180 TQYRYSLGVR SQAEMVAALR RLNVECEAVG PEARLPISMR STRALGKHTE VSCKKSSQFL 190 200 210 220 230 240 SGLLYLGAIG ERDLEIDVVD HITAPSMVHT TINNLAHAGV AVEYDAAFRR FFVPGRDRFK 250 260 270 280 290 300 PSEFTVGADP ASTAAILALC GSLASDVTLN GFFEEELGSG AVIRYLTDTG TLIDELPGNR 310 320 330 340 350 360 IRIRGGASIR AQDFDGSLAP DAVPALAGRA AFAEGTSTFY NIEHIRYKES DRISDFRREL 370 380 390 400 410 420 DKLGVRSEEK LDQLIIHGNP RSYRGGAVVD GHYDHGLIMA LTTIGLHCEH PVLIKEPHHV 430 440 450 GQTYPDYFAD IGSIGANVDG LIYPNVAAAR A

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH34676.1.
RefSeq	YP_107312.1.
3D structure databases
SMR	Q63X54. Positions 12-439.
ModBase	Search...
Genome annotation databases
GeneID	3094478.
GenomeReviews	Gene locus BPSL0683 in contig BX571965_GR.
KEGG	bps:BPSL0683.
NMPDR	fig|272560.3.peg.2516.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	DVEIRAD.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL0683-MONOMER.
BRENDA	2.5.1.19. 275934.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA1_BURPS

Accession

Primary (citable) accession number: Q63X54

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	October 25, 2004
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)