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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi274 – 2741Manganese 2UniRule annotation
Metal bindingi279 – 2791Manganese 1UniRule annotation
Metal bindingi279 – 2791Manganese 2UniRule annotation
Active sitei286 – 2861UniRule annotation
Metal bindingi297 – 2971Manganese 2UniRule annotation
Metal bindingi356 – 3561Manganese 1UniRule annotation
Metal bindingi358 – 3581Manganese 1UniRule annotation
Metal bindingi358 – 3581Manganese 2UniRule annotation
Active sitei360 – 3601UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-984-MONOMER.

Protein family/group databases

MEROPSiM17.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:BPSL0965
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000000605 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Probable cytosol aminopeptidasePRO_0000165733Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272560.BPSL0965.

Structurei

3D structure databases

ProteinModelPortaliQ63WC3.
SMRiQ63WC3. Positions 20-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiPRALKKV.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63WC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSIKGCDW SKGTANGFLT GKSDCIVLGV FEAQTLSGAA LDIDEATKGL
60 70 80 90 100
VSRVIKAGDI DGKLGKTLFL HEVSGIGASR VLLVGLGRQD AFSQKAYGDA
110 120 130 140 150
AKAAWRALLG TKVVQVTFTL AQLPVPERAS DWGVRAAILA LRNETYKFTQ
160 170 180 190 200
MKSKPDAGAP ALKRVVFSVD PADDKAAKVA AKQAVALANG MDLTRDLGNL
210 220 230 240 250
PGNVCTPTYL ANTAKKIAKD WGLKVDVLGL KQIQALKMGS FLSVAKGSVE
260 270 280 290 300
PPQFIVLQYR GAAAKAAPVV LVGKGITFDS GGISLKPGEG MDEMKYDMCG
310 320 330 340 350
AGSVLGTMRA VAEMGLKVNV VAIVPTCENM PAGNANKPGD IVTSMKGLTI
360 370 380 390 400
EVLNTDAEGR LILCDALTYA ERFKPAAVID VATLTGACII ALGHHNTGLF
410 420 430 440 450
SKDDALAGEL LDASREAGDP AWRLPLDDEY QDQLKSNFAD LANIGGRPAG
460 470 480 490 500
SVTAACFLSR FAENYPWAHL DIAGTAWKSG AAKGATGRPV PLLAQFLIDR

AGA
Length:503
Mass (Da):52,653
Last modified:October 24, 2004 - v1
Checksum:iA641EACDE75CEC4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH34961.1.
RefSeqiYP_107593.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH34961; CAH34961; BPSL0965.
GeneIDi3092640.
KEGGibps:BPSL0965.
PATRICi19261635. VBIBurPse99623_1046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH34961.1.
RefSeqiYP_107593.1. NC_006350.1.

3D structure databases

ProteinModelPortaliQ63WC3.
SMRiQ63WC3. Positions 20-502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSL0965.

Protein family/group databases

MEROPSiM17.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH34961; CAH34961; BPSL0965.
GeneIDi3092640.
KEGGibps:BPSL0965.
PATRICi19261635. VBIBurPse99623_1046.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243132.
KOiK01255.
OMAiPRALKKV.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-984-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiAMPA_BURPS
AccessioniPrimary (citable) accession number: Q63WC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2004
Last sequence update: October 24, 2004
Last modified: January 6, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.