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Q63WC3 (AMPA_BURPS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:BPSL0965
OrganismBurkholderia pseudomallei (strain K96243) [Reference proteome] [HAMAP]
Taxonomic identifier272560 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_0000165733

Sites

Active site2861 Potential
Active site3601 Potential
Metal binding2741Manganese 2 By similarity
Metal binding2791Manganese 1 By similarity
Metal binding2791Manganese 2 By similarity
Metal binding2971Manganese 2 By similarity
Metal binding3561Manganese 1 By similarity
Metal binding3581Manganese 1 By similarity
Metal binding3581Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q63WC3 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: A641EACDE75CEC4F

FASTA50352,653
        10         20         30         40         50         60 
MDFSIKGCDW SKGTANGFLT GKSDCIVLGV FEAQTLSGAA LDIDEATKGL VSRVIKAGDI 

        70         80         90        100        110        120 
DGKLGKTLFL HEVSGIGASR VLLVGLGRQD AFSQKAYGDA AKAAWRALLG TKVVQVTFTL 

       130        140        150        160        170        180 
AQLPVPERAS DWGVRAAILA LRNETYKFTQ MKSKPDAGAP ALKRVVFSVD PADDKAAKVA 

       190        200        210        220        230        240 
AKQAVALANG MDLTRDLGNL PGNVCTPTYL ANTAKKIAKD WGLKVDVLGL KQIQALKMGS 

       250        260        270        280        290        300 
FLSVAKGSVE PPQFIVLQYR GAAAKAAPVV LVGKGITFDS GGISLKPGEG MDEMKYDMCG 

       310        320        330        340        350        360 
AGSVLGTMRA VAEMGLKVNV VAIVPTCENM PAGNANKPGD IVTSMKGLTI EVLNTDAEGR 

       370        380        390        400        410        420 
LILCDALTYA ERFKPAAVID VATLTGACII ALGHHNTGLF SKDDALAGEL LDASREAGDP 

       430        440        450        460        470        480 
AWRLPLDDEY QDQLKSNFAD LANIGGRPAG SVTAACFLSR FAENYPWAHL DIAGTAWKSG 

       490        500 
AAKGATGRPV PLLAQFLIDR AGA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571965 Genomic DNA. Translation: CAH34961.1.
RefSeqYP_107593.1. NC_006350.1.

3D structure databases

ProteinModelPortalQ63WC3.
SMRQ63WC3. Positions 20-502.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272560.BPSL0965.

Protein family/group databases

MEROPSM17.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH34961; CAH34961; BPSL0965.
GeneID3092640.
KEGGbps:BPSL0965.
PATRIC19261635. VBIBurPse99623_1046.

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243132.
KOK01255.
OMAAFISHFS.
OrthoDBEOG6FV8B3.

Enzyme and pathway databases

BioCycBPSE272560:GJNI-984-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_BURPS
AccessionPrimary (citable) accession number: Q63WC3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: October 25, 2004
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries