Reviewed, UniProtKB/Swiss-Prot Q63VT1 (HEM6_BURPS)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	BPSL1163

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_0000109890
Regions
	Region	55 – 64	10	Important for dimerization By similarity
	Region	115 – 117	3	Substrate binding By similarity
	Region	247 – 282	36	Important for dimerization By similarity
	Region	265 – 270	6	Substrate binding By similarity
Sites
	Active site	113	1	Proton donor By similarity
	Binding site	99	1	Substrate By similarity
	Site	182	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63VT1-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 7A5FCC82FA3139A1	FASTA	307	34,569
	10 20 30 40 50 60 MTDSTYDVNR VRAYLQGLQM RIADALGAFD GTPLAADTWR RGPGERLRGG GCTRILEAGG 70 80 90 100 110 120 FFERAGIGFS DVAGDALPPS ASASRPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI 130 140 150 160 170 180 ATKPGEAPVF WFGGGMDLTP IYGFEEDARH FHRTCRAALE PFGAELYPRF KKWCDDYFFL 190 200 210 220 230 240 KHRNEARGIG GIFFDDFSEL GFERSFEMLQ SVGDAFLPSY LPIVERRRDT PYGERERAFQ 250 260 270 280 290 300 AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILLSMPPTAG WRYDWHPDPG TPEARLQSEF LVPRDWA

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH35158.1.
RefSeq	YP_107785.1.
3D structure databases
SMR	Q63VT1. Positions 5-307.
ModBase	Search...
Genome annotation databases
GeneID	3092344.
GenomeReviews	Gene locus BPSL1163 in contig BX571965_GR.
KEGG	bps:BPSL1163.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631180.
OMA	HMNTRFV.
PhylomeDB	Q63VT1.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL1163-MONOMER.
BRENDA	1.3.3.3. 275934.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_BURPS

Accession

Primary (citable) accession number: Q63VT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 25, 2004
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)