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Protein

Uracil phosphoribosyltransferase

Gene

upp

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.UniRule annotation

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP.UniRule annotation

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase (upp)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 8515-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei110 – 11015-phospho-alpha-D-ribose 1-diphosphateUniRule annotation
Binding sitei200 – 2001Uracil; via amide nitrogenUniRule annotation
Binding sitei206 – 20615-phospho-alpha-D-ribose 1-diphosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-1185-MONOMER.
UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil phosphoribosyltransferaseUniRule annotation (EC:2.4.2.9UniRule annotation)
Alternative name(s):
UMP pyrophosphorylaseUniRule annotation
UPRTaseUniRule annotation
Gene namesi
Name:uppUniRule annotation
Ordered Locus Names:BPSL1166
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000000605 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Uracil phosphoribosyltransferasePRO_0000120809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272560.BPSL1166.

Structurei

Secondary structure

1
216
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 134Combined sources
Helixi16 – 2611Combined sources
Helixi32 – 5019Combined sources
Turni51 – 533Combined sources
Beta strandi56 – 627Combined sources
Beta strandi67 – 737Combined sources
Helixi75 – 773Combined sources
Beta strandi78 – 847Combined sources
Turni85 – 873Combined sources
Helixi88 – 9710Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi105 – 1073Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi119 – 1224Combined sources
Beta strandi130 – 1345Combined sources
Beta strandi136 – 1416Combined sources
Helixi142 – 15211Combined sources
Turni153 – 1553Combined sources
Helixi158 – 1603Combined sources
Beta strandi161 – 1644Combined sources
Beta strandi166 – 1683Combined sources
Helixi170 – 17910Combined sources
Beta strandi184 – 1907Combined sources
Beta strandi200 – 2034Combined sources
Helixi207 – 2126Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DMPX-ray2.60A/B/C/D1-216[»]
ProteinModelPortaliQ63VS8.
SMRiQ63VS8. Positions 1-213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63VS8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 14395-phospho-alpha-D-ribose 1-diphosphate bindingUniRule annotation
Regioni205 – 2073Uracil bindingUniRule annotation

Sequence similaritiesi

Belongs to the UPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
HOGENOMiHOG000262754.
KOiK00761.
OMAiNTHLWIA.
OrthoDBiEOG6HF5WX.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B.
InterProiIPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63VS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQDSRFPNL FILDHPLIQH KLTHMRDKDT STRTFRELLR EITLLMGYEI
60 70 80 90 100
TRNLPITTKR VETPLVEIDA PVIAGKKLAI VPVLRAGVGM SDGLLELIPS
110 120 130 140 150
ARVGHIGVYR ADDHRPVEYL VRLPDLEDRI FILCDPMVAT GYSAAHAIDV
160 170 180 190 200
LKRRGVPGER LMFLALVAAP EGVQVFQDAH PDVKLYVASL DSHLDDHAYI
210
VPGLGDAGDR LFGTKN
Length:216
Mass (Da):24,075
Last modified:October 25, 2004 - v1
Checksum:iDA8F1B3A2ECFB56B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH35161.1.
RefSeqiWP_004186446.1. NZ_CP009538.1.
YP_107788.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH35161; CAH35161; BPSL1166.
GeneIDi3091557.
KEGGibps:BPSL1166.
PATRICi19262107. VBIBurPse99623_1285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH35161.1.
RefSeqiWP_004186446.1. NZ_CP009538.1.
YP_107788.1. NC_006350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DMPX-ray2.60A/B/C/D1-216[»]
ProteinModelPortaliQ63VS8.
SMRiQ63VS8. Positions 1-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSL1166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH35161; CAH35161; BPSL1166.
GeneIDi3091557.
KEGGibps:BPSL1166.
PATRICi19262107. VBIBurPse99623_1285.

Phylogenomic databases

eggNOGiENOG4105CZ5. Bacteria.
COG0035. LUCA.
HOGENOMiHOG000262754.
KOiK00761.
OMAiNTHLWIA.
OrthoDBiEOG6HF5WX.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.
BioCyciBPSE272560:GJNI-1185-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ63VS8.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01218_B. Upp_B.
InterProiIPR029057. PRTase-like.
IPR005765. Ura_phspho_trans.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01091. upp. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.
  2. "2.6 A crystal structure of uracil phosphoribosyltransferase from Burkholderia pseudomallei."
    Seattle structural genomics center for infectious disease (SSGCID)
    Submitted (JUL-2008) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

Entry informationi

Entry nameiUPP_BURPS
AccessioniPrimary (citable) accession number: Q63VS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 25, 2004
Last modified: January 20, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.