Reviewed, UniProtKB/Swiss-Prot Q63U72 (OTCC_BURPS)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase, catabolic
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	BPSL1744

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000112903
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	273 – 276	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63U72-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 470CDF2CA9D0FA6B	FASTA	336	38,017
	10 20 30 40 50 60 MSFNLHNRNL LSLIHHNARE LRYLLDLARD LKRAKYSGTE QPRLLRKNIA LIFEKTSTRT 70 80 90 100 110 120 RCAFEVAAYD QGANVTYIDP ASSQIGHKES MRDTARVLGR MYDAIEYRGF SQEIVEELAH 130 140 150 160 170 180 HAGVPVFNGL TDEYHPTQML ADVMTMREHS DKPLHDIRYA YLGDARNNMG NSLLLIGAKL 190 200 210 220 230 240 GMDVRIGAPK SLWPAADFIA QCEAFAAESG ARLTLTEDPY EAVKGVDFIH TDVWVSMGEP 250 260 270 280 290 300 VEAWDERINA LLPYQVNRKL IESTGNPRTR FMHCLPSFHN CETKVGKQIA ERYPHLKDGI 310 320 330 EVTDEVFESP RCIAFEQAEN RMHTIKAVLV STLGGI

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH35743.1.
RefSeq	YP_108344.1.
3D structure databases
SMR	Q63U72. Positions 2-336.
ModBase	Search...
Genome annotation databases
GeneID	3092910.
GenomeReviews	Gene locus BPSL1744 in contig BX571965_GR.
KEGG	bps:BPSL1744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	GIEYRGY.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL1744-MONOMER.
BRENDA	2.1.3.3. 275934.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_BURPS

Accession

Primary (citable) accession number: Q63U72

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)