1,4-alpha-glucan branching enzyme GlgB - Burkholderia pseudomallei

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Q63T91 (GLGB_BURPS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	BPSL2076

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 738

738

1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685

PRO_0000188689

Sites

Active site

417

Nucleophile By similarity

Active site

472

Proton donor By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q63T91 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: B1AFA639ECEF4C1D
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FASTA

738

81,016

        10         20         30         40         50         60 
MTDAPFDRAD IDALLGARHP DPFACLGPHR VGDATVVRTL LPGALRVRAI AAGGGVLGEL 

        70         80         90        100        110        120 
RQVDPAGCFA GALPDGQERG ERPRYRLSID WPDARQDVED AYAFGTLLDE DALARFAAGD 

       130        140        150        160        170        180 
PRAALACLGA RALDMDGVPG VRFAVWAPGA SRVSVVGDFN GWDARRHPMR LRRPWGVWEL 

       190        200        210        220        230        240 
FVPRIGAGER YKFALRARDG AALPLKADPC ACRTEAPPRT ASIVADLDAL ERFGWHDDAW 

       250        260        270        280        290        300 
LRARASLDLA HAPVSIYEVH PESWLRVAAE GNRSATWDEL AQRLIPYAAG MGFSHVELTP 

       310        320        330        340        350        360 
IAEYPFGGSW GYQSLSPFAP SARFGPPEGF ARFVEHAHAA GLGVIVDWVP AHFPDDPHGL 

       370        380        390        400        410        420 
GKFDGTALFE HADPREGWHP DWHTHVFNVG RREVGAFLIA SALAWAHRYH VDGIRVDAVA 

       430        440        450        460        470        480 
SMLYRDYSRA AGEWVPNVYG GRENLESIAF LKHFNDTLHG PAAPPGVATF AEESTAWPGV 

       490        500        510        520        530        540 
TAPTAEHGLG FDFKWNMGWM HDTLAYLRED PIHRRHHHDR LTFGLVYAFS ERFVLPLSHD 

       550        560        570        580        590        600 
EVVHGKGSLA AKMPGDAWQR LANLRAYFGF MWAHPGKKLL FMGGEFAQWG EFAHDATPQW 

       610        620        630        640        650        660 
DLLDAPAHRG VQRLVRDLNR LHAAEPALHA LDDRPAGFAW LVGDDRNNSV FAFVRRDDAG 

       670        680        690        700        710        720 
RMLVAVCNFT PVPRTDYRLG LPAPGRWAEV LNTDGAAYGG TDAGNGGAVQ ADEIPAHGER 

       730 
WSAALRLPPL ATLWLRPA

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References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei."
Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L.

Parkhill J.
Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH36077.1.
RefSeq	YP_108675.1. NC_006350.1.
3D structure databases
ProteinModelPortal	Q63T91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3092569.
GenomeReviews	Gene locus BPSL2076 in contig BX571965_GR.
KEGG	bps:BPSL2076.
NMPDR	fig\|272560.3.peg.3901.
PATRIC	19264269. VBIBurPse99623_2351.
Phylogenomic databases
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2076-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURPS

Accession

Primary (citable) accession number: Q63T91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents