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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

BPSL2194

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021SubstrateBy similarity
Metal bindingi103 – 1031Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-2240-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:BPSL2194
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000000605 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_0000209606Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi272560.BPSL2194.

Structurei

3D structure databases

ProteinModelPortaliQ63SX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 834Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiCOG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiLQICRQS.
OrthoDBiEOG6Z3KKK.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63SX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFATTDLCD AHEDRLAAGT LRVLEPVFRP FGGVRRFAGP AATLKLFEDN
60 70 80 90 100
SLVRTALEQD GAGRVLVVDG GGSLRCALVG GNLGKLAEKN GWAGIVVNGC
110 120 130 140 150
VRDSDELAEC RVGVLALAAH PRKSDKRGAG VSDAPVDVRG TRIVPGDWIY
160
ADADGVLVSD DALLE
Length:165
Mass (Da):17,301
Last modified:October 25, 2004 - v1
Checksum:iACCDBB82E0923ACA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH36196.1.
RefSeqiWP_004191744.1. NZ_CP009538.1.
YP_108789.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH36196; CAH36196; BPSL2194.
GeneIDi3093172.
KEGGibps:BPSL2194.
PATRICi19264551. VBIBurPse99623_2491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH36196.1.
RefSeqiWP_004191744.1. NZ_CP009538.1.
YP_108789.1. NC_006350.1.

3D structure databases

ProteinModelPortaliQ63SX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSL2194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH36196; CAH36196; BPSL2194.
GeneIDi3093172.
KEGGibps:BPSL2194.
PATRICi19264551. VBIBurPse99623_2491.

Phylogenomic databases

eggNOGiCOG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiLQICRQS.
OrthoDBiEOG6Z3KKK.

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-2240-MONOMER.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiRRAAH_BURPS
AccessioniPrimary (citable) accession number: Q63SX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 25, 2004
Last modified: April 13, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.