Reviewed, UniProtKB/Swiss-Prot Q63SP9 (KDSA1_BURPS)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase 1
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase 1
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase 1
    KDO-8-phosphate synthetase 1
      Short name=KDO 8-P synthase 1
      Short name=KDOPS 1

Gene names

Name:	kdsA1
Ordered Locus Names:	BPSL2271

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	2-dehydro-3-deoxyphosphooctonate aldolase 1 HAMAP MF_00056		PRO_0000187110

Sequences

	Sequence		Length	Mass (Da)
	Q63SP9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 152D3B5E894DEFBD	FASTA	284	30,570
	10 20 30 40 50 60 MNLAGFEVGL DKPFFLIAGT CVVESEQMTI DTAGRLKEIC ATLGVPFIYK SSYDKANRSS 70 80 90 100 110 120 GKSFRGLGMD EGLRILAEVK RQLNVPVLTD VHEIDEIAPV AAVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 RACAQSGKPV NIKKGQFLAP HDMKNVIDKA RDAARDAGLS EDRFMACERG VSFGYNNLVS 190 200 210 220 230 240 DMRSLAIMRE TGAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAALAT GVAGLFMETH 250 260 270 280 PNPAEAKSDG PNAVPLGRMA ALLETLVTLD RAVKRVPFLE NDFN

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH36274.1.
RefSeq	YP_108867.1.
3D structure databases
SMR	Q63SP9. Positions 12-275.
ModBase	Search...
Genome annotation databases
GeneID	3091613.
GenomeReviews	Gene locus BPSL2271 in contig BX571965_GR.
KEGG	bps:BPSL2271.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG575990.
OMA	LAPWDMG.
PhylomeDB	Q63SP9.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2271-MONOMER.
BRENDA	2.5.1.55. 275934.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA1_BURPS

Accession

Primary (citable) accession number: Q63SP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)