Reviewed, UniProtKB/Swiss-Prot Q63SI0 (HUTI_BURPS)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BPSL2340

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Imidazolonepropionase HAMAP MF_00372		PRO_0000306450
Sites
	Metal binding	68	1	Zinc or iron By similarity
	Metal binding	70	1	Zinc or iron By similarity
	Metal binding	238	1	Zinc or iron By similarity
	Metal binding	313	1	Zinc or iron By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63SI0-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 0D598C798DB8E369	FASTA	407	43,787
	10 20 30 40 50 60 MKSILWHNLK LCAHGDPNDT IADAAIAVNG DGTIAWTGRA SDVPAGYVHW PREDLRGAWV 70 80 90 100 110 120 TPGLVDCHTH LVYGGQRADE FAQRLAGASY EEIAQRGGGI VSTVRATRDA SEAALFEQAC 130 140 150 160 170 180 ARLRPLLAEG VTAIEIKSGY GLELASERRM LRVARQLGER FPVSVYTTFL GAHALPPEYA 190 200 210 220 230 240 GRADEYIDEV CERMLPALAD EGLVDAVDVF CERIGFTLAQ SERVFEAAAR RGLPVKMHAE 250 260 270 280 290 300 QLSNGGGSAL AARYRALSAD HLEYLDAAGV AAMRASGTTA VLLPGAYYFI RETKLPPIDL 310 320 330 340 350 360 LRRHGVPIAL ATDHNPGTSP LTSLLLTMNM GCTVFKLTVQ EALLGVTRHA AAALGASDRH 370 380 390 400 GSLAPGRQAD FAVWPVSTLA ELAYWFGRPL CERVVKGGVT VFTRDAR

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571965 Genomic DNA. Translation: CAH36343.1.
RefSeq	YP_108936.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3092653.
GenomeReviews	Gene locus BPSL2340 in contig BX571965_GR.
KEGG	bps:BPSL2340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63SI0.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2340-MON.
BRENDA	3.5.2.7. 275934.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURPS

Accession

Primary (citable) accession number: Q63SI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)