Q63RX6 (METE_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BPSL2545

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071607
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63RX6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 203C1A3E04661D2D	FASTA	764	84,451
	10 20 30 40 50 60 MTTAHILGFP RIGAQRELKF ALERYWRDGA SADAERALVD TGRALRAEHW RIERDAGLDC 70 80 90 100 110 120 VTVGDFAWYD HVLTTLAHVG GLPRRFGFDA RALTLADYFA AARGNAAQPA MEMTKWFDTN 130 140 150 160 170 180 YHYLVPEYSP ATTFGPGVEW LFDEVREARA LGYRAKAALV GPLTLLWLGK ARDGLVERLA 190 200 210 220 230 240 LLPRLVPAYR ALLARLREAG VDWVQIEEPI FSLDLPDAWR DAARPTYEAL APGAPKLLVA 250 260 270 280 290 300 TYFDDASEHA ALLKALPVAG LHIDLVRADA QLDAFVADYP ADKVLSCGIV DGRNVWRNDL 310 320 330 340 350 360 DRSLARLAPV RDALGERLWV ATSCSLLHVP VDLAHEPRLD EELKTWLAFA AQKTREVAAL 370 380 390 400 410 420 RDALVKGRAA VAAEFDDAAA AAAARRTSAR IHNPLVKRRV AALTDADARR ASAYSVRAAA 430 440 450 460 470 480 QRARFGLPLL PTTTIGSFPQ TPEIRRARAA FKQGVLDHLG YLEAMREQVR IAIDKQLAYG 490 500 510 520 530 540 LDVLVHGEAE RNDMVEYFGE LLWGFAITSN GWVQSYGSRC VKPPLVYGDV YLPEPMTVGW 550 560 570 580 590 600 ASYAQSLSAK PVKGMLTGPV TMLQWSFVRD DQPRATTALQ IALALRQETL DLEKAGIGMI 610 620 630 640 650 660 QIDEPALREG LPLKARERAA YLDWAVRAFG IAASGVADDT QIHTHMCYSE FGDILPSIAA 670 680 690 700 710 720 LDADVISIET TRSNMELLDA FETFDYPNEI GPGVYDIHSP RVPDADEIER LILLALERIP 730 740 750 760 AQRLWVNPDC GLKTREWRQV DAALAAMVDA AKRVRQKVEE EAPA

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH36552.1.
RefSeq	YP_109141.1. NC_006350.1.
3D structure databases
HSSP	HSSP built from PDB template 1U1J based on UniProtKB O50008.
ProteinModelPortal	Q63RX6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3092004.
GenomeReviews	Gene locus BPSL2545 in contig BX571965_GR.
KEGG	bps:BPSL2545.
NMPDR	fig|272560.3.peg.4900.
PATRIC	19265399. VBIBurPse99623_2902.
Phylogenomic databases
HOGENOM	HBG287495.
OMA	NIWANDF.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2545-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURPS

Accession

Primary (citable) accession number: Q63RX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program