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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
Binding sitei120 – 1201S-adenosyl-L-methionineUniRule annotation
Binding sitei147 – 1471S-adenosyl-L-methionineUniRule annotation
Active sitei170 – 1701By similarity
Binding sitei170 – 1701S-adenosyl-L-methionineUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Binding sitei206 – 2061SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-2715-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BPSL2642
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000000605 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265tRNA (guanine-N(7)-)-methyltransferasePRO_0000171311Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272560.BPSL2642.

Structurei

3D structure databases

ProteinModelPortaliQ63RN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni241 – 2444Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63RN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHDDDPNAP GAPHDDDATA APASATRAAP AAGDDDDANP LHLRRIRSFV
60 70 80 90 100
TRAGRVSTGQ RRAIDELGPR FVIPYGSAQP DWDAIFGRRA PRVLEIGFGM
110 120 130 140 150
GASTAEIAAL RPGDDFIGVE VHEPGVGALL KLIGEQQLSN IRIIQHDAVE
160 170 180 190 200
VLAQMIAPDS LDGVHIFFPD PWHKARHHKR RLIQPPFVAQ LAAHLKPGAY
210 220 230 240 250
LHCATDWQNY AEQMLEVLSA DPSLENTAQD YAPRPGYRPV TKFERRGLRL
260
GHGVWDLVFR KKHAG
Length:265
Mass (Da):29,102
Last modified:October 24, 2004 - v1
Checksum:iD19E15399EE03B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH36650.1.
RefSeqiYP_109238.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH36650; CAH36650; BPSL2642.
GeneIDi3093291.
KEGGibps:BPSL2642.
PATRICi19265631. VBIBurPse99623_3012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571965 Genomic DNA. Translation: CAH36650.1.
RefSeqiYP_109238.1. NC_006350.1.

3D structure databases

ProteinModelPortaliQ63RN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSL2642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH36650; CAH36650; BPSL2642.
GeneIDi3093291.
KEGGibps:BPSL2642.
PATRICi19265631. VBIBurPse99623_3012.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciBPSE272560:GJNI-2715-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiTRMB_BURPS
AccessioniPrimary (citable) accession number: Q63RN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 14, 2005
Last sequence update: October 24, 2004
Last modified: January 6, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.