Q63RA1 (KDSA2_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase 2
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase 2
KDO-8-phosphate synthase 2
Short name=KDO 8-P synthase 2
Short name=KDOPS 2
Phospho-2-dehydro-3-deoxyoctonate aldolase 2

Gene names

Name:	kdsA2
Ordered Locus Names:	BPSL2772

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	2-dehydro-3-deoxyphosphooctonate aldolase 2 HAMAP MF_00056		PRO_0000187111

Sequences

	Sequence		Length	Mass (Da)
	Q63RA1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FACA5DC907F1E273	FASTA	281	30,213
	10 20 30 40 50 60 MNVAISPGVT AGNSLPFVLF GGINVLESLD FTLDVCGEYV AVTRKLGIPF VFKASFDKAN 70 80 90 100 110 120 RSSIHSYRGV GLDEGLKIFA EVKARFGVPV ITDVHEVEQA APVAEIADVL QVPAFLARQT 130 140 150 160 170 180 DLVVAIAKAG KPVNVKKPQF MSPTQLKHVV SKCGEVGNDR VMLCERGSSF GYDNLVVDML 190 200 210 220 230 240 GFRQMAETTG GCPVIFDVTH SLQCRDPLGD ASGGRRRQVL DLARAGIAVG IAGLFLEAHP 250 260 270 280 DPDRARCDGP SALPLHQLEG FLSQMKAIDD LVKRMPALEI R

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571965 Genomic DNA. Translation: CAH36781.1.
RefSeq	YP_109367.1. NC_006350.1.
3D structure databases
ProteinModelPortal	Q63RA1.
SMR	Q63RA1. Positions 9-279.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3093882.
GenomeReviews	Gene locus BPSL2772 in contig BX571965_GR.
KEGG	bps:BPSL2772.
PATRIC	19265941. VBIBurPse99623_3166.
Phylogenomic databases
HOGENOM	HBG575990.
OMA	NFFLMAG.
PhylomeDB	Q63RA1.
ProtClustDB	PRK12457.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2772-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA2_BURPS

Accession

Primary (citable) accession number: Q63RA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program