Reviewed, UniProtKB/Swiss-Prot Q63R43 (HEMH_BURPS)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BPSL2831

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Ferrochelatase HAMAP MF_00323		PRO_0000175124
Sites
	Metal binding	226	1	Iron By similarity
	Metal binding	307	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63R43-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: CB37193FB9181959	FASTA	367	40,571
	10 20 30 40 50 60 MSFDSVPRHA LSMRFDLEPP SHASAAHRVA VLLVNLGTPD APTPRAVRRY LAQFLSDPRV 70 80 90 100 110 120 VEIPQLVWQV ILRTLILPLR GRASAKKYAA VWLPEGSPLR VYTERQVESV KPLFAANGYR 130 140 150 160 170 180 VIVDYAMRYG TPSIADVLAQ LKRAGAERVL LLPMYPQYSS STTATAFDAA FAALGRMRNQ 190 200 210 220 230 240 PEVRTVRHYA DHPAYIHALA EQVRQYWAAH GRPAFDAGDK LVLSFHGVPK RTLDLGDPYH 250 260 270 280 290 300 DQCQQTAALL MSALGLTTFE CRVTFQSRFG KAEWLQPYTA PTLKELGAAG VRRADVFCPG 310 320 330 340 350 360 FTADCLETIE EIGIEVRDEF VHGGGKEFHR IPCLNASPAW IAALGEIAAE NLQGWPVRVA MAPEAVS

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571965 Genomic DNA. Translation: CAH36841.1.
RefSeq	YP_109425.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3093284.
GenomeReviews	Gene locus BPSL2831 in contig BX571965_GR.
KEGG	bps:BPSL2831.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63R43.
OMA	TIEEIGM.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL2831-MON.
BRENDA	4.99.1.1. 275934.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURPS

Accession

Primary (citable) accession number: Q63R43

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)