Reviewed, UniProtKB/Swiss-Prot Q63PS2 (CHEB1_BURPS)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	BPSL3301

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225445
Regions
	Domain	9 – 126	118	Response regulatory
	Domain	168 – 360	193	CheB-type methylesterase
Sites
	Active site	180	1	By similarity
	Active site	206	1	By similarity
	Active site	302	1	By similarity
Amino acid modifications
	Modified residue	60	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63PS2-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FA7997711D5947C8	FASTA	367	39,353
	10 20 30 40 50 60 MNAVQKKIKV LCVDDSALIR SLMTEIINSQ PDMEVCATAP DPLVARELIK QHNPDVLTLD 70 80 90 100 110 120 VEMPRMDGLD FLEKLMRLRP MPVVMVSSLT ERGSEITLRA LELGAVDFVT KPRVGIRDGM 130 140 150 160 170 180 LDYSEKLADK VRAASRARVR QNPQPHAAAA AAAHGHAGAA APLINNPLVS TEKLIIVGAS 190 200 210 220 230 240 TGGTEAIREV LTPLPPDAPA VLIAQHMPPG FTRSFAQRLN GLCRISVKEA EHGERVLPGH 250 260 270 280 290 300 AYIAPGHAHL LLARSGANYI AHLSDEPPVN RHRPSVDVLF RSAAQHAGKN ALGVILTGMG 310 320 330 340 350 360 RDGAAGLLEM KKAGAYTFAQ DEASCVVFGM PREAIAMGGV DDVAPLSDMS RRIMARLASM GDRVQRV

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571965 Genomic DNA. Translation: CAH37314.1.
RefSeq	YP_109897.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3092815.
GenomeReviews	Gene locus BPSL3301 in contig BX571965_GR.
KEGG	bps:BPSL3301.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63PS2.
OMA	IVIAQHI.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL3301-MON.
BRENDA	3.1.1.61. 275934.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_BURPS

Accession

Primary (citable) accession number: Q63PS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)