Reviewed, UniProtKB/Swiss-Prot Q63PL4 (GCST_BURPS)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BPSL3360

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP]

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Aminomethyltransferase HAMAP MF_00259		PRO_0000122550

Sequences

	Sequence		Length	Mass (Da)
	Q63PL4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 0EE9681AF1660350	FASTA	372	40,093
	10 20 30 40 50 60 MTVLKTTPLH AAHRALNARM VDFGGWDMPV NYGSQIEEHQ AVRTDAGMFD VSHMCVVDFT 70 80 90 100 110 120 GPRVRAFFEH AIANNVAKLQ TPGKALYSCL LNPQGGVIDD LIVYYFTEEF FRVVVNAGTA 130 140 150 160 170 180 EKDIAWFNQL NEQGGFGLTI APRRDFAIVA AQGPNARAKV WDTVPCARAA TSELKPFNAA 190 200 210 220 230 240 QVAGTPFGDL TVARTGYTGE DGFEIIVPAT HVEALWNALA ERGVRPCGLG ARDTLRLEAG 250 260 270 280 290 300 MNLYGQDMDE SVSPLDAGLA WTVDLSAPRA FVGRDALEAH GSRAAFVGLI LQKENGRAGG 310 320 330 340 350 360 VLRAHQKVAT PHGEGEITSG TFSPSMQESI AFARVPKDVA IGDTVHVQIR DKQLPARVVK 370 LPFVRNGKVL AA

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571965 Genomic DNA. Translation: CAH37373.1.
RefSeq	YP_109955.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3092626.
GenomeReviews	Gene locus BPSL3360 in contig BX571965_GR.
KEGG	bps:BPSL3360.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63PL4.
OMA	VEIRGKW.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSL3360-MON.
BRENDA	2.1.2.10. 275934.
Family and domain databases
HAMAP	MF_00259. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BURPS

Accession

Primary (citable) accession number: Q63PL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)