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Q63NG7

- HYPRE_BURPS

UniProt

Q63NG7 - HYPRE_BURPS

Protein

4-hydroxyproline epimerase

Gene

BPSS0332

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases.

    Catalytic activityi

    Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

    Enzyme regulationi

    Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

    Kineticsi

    1. KM=14.4 mM for 4-hydroxy-L-proline1 Publication
    2. KM=17.1 mM for 4-hydroxy-D-proline1 Publication

    Vmax=2.1 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)1 Publication

    Vmax=2.4 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Proton acceptorBy similarity
    Active sitei236 – 2361Proton donorBy similarity

    GO - Molecular functioni

    1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
    2. proline racemase activity Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciBPSE272560:GJNI-3867-MONOMER.
    BRENDAi5.1.1.8. 1031.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyproline epimerase (EC:5.1.1.8)
    Alternative name(s):
    Hydroxyproline-2-epimerase
    Short name:
    BpHyPRE
    Gene namesi
    Ordered Locus Names:BPSS0332
    OrganismiBurkholderia pseudomallei (strain K96243)
    Taxonomic identifieri272560 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000000605: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3103104-hydroxyproline epimerasePRO_0000354034Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi272560.BPSS0332.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63NG7.
    SMRiQ63NG7. Positions 1-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3938.
    HOGENOMiHOG000084335.
    KOiK12658.
    OMAiWIRQALM.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q63NG7-1 [UniParc]FASTAAdd to Basket

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    MKHIHIIDSH TGGEPTRVVV SGFPALGGGT MAERLAVLAR EHDRYRAACI    50
    LEPRGSDVLV GALLCEPVSA GAAAGVIFFN NAGYLGMCGH GTIGLVRTLH 100
    HMGRIGPGVH RIETPVGDVE ATLHDDLSVS VRNVLAYRHA KDVVVDVPGH 150
    GAVTGDVAWG GNWFFLVSDH GQRVAGENVA ALAAYASAVR AALERAGVTG 200
    RDGAPIDHIE LFADDPEYDS RSFVLCPGHA YDRSPCGTGT SAKLACLAAD 250
    GKLAAGVTWR QASVIGSVFS ASYAAAEGGV VPTIRGSAHL SAEATLVIED 300
    DDPFGWGIAS 310
    Length:310
    Mass (Da):32,118
    Last modified:October 25, 2004 - v1
    Checksum:i4315A2A48443A002
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495345 Genomic DNA. Translation: ABS82397.1.
    BX571966 Genomic DNA. Translation: CAH37780.1.
    RefSeqiWP_004523447.1. NC_006351.1.
    YP_110352.1. NC_006351.1.

    Genome annotation databases

    EnsemblBacteriaiCAH37780; CAH37780; BPSS0332.
    GeneIDi3097013.
    KEGGibps:BPSS0332.
    PATRICi19268294. VBIBurPse99623_4324.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495345 Genomic DNA. Translation: ABS82397.1 .
    BX571966 Genomic DNA. Translation: CAH37780.1 .
    RefSeqi WP_004523447.1. NC_006351.1.
    YP_110352.1. NC_006351.1.

    3D structure databases

    ProteinModelPortali Q63NG7.
    SMRi Q63NG7. Positions 1-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272560.BPSS0332.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH37780 ; CAH37780 ; BPSS0332 .
    GeneIDi 3097013.
    KEGGi bps:BPSS0332.
    PATRICi 19268294. VBIBurPse99623_4324.

    Phylogenomic databases

    eggNOGi COG3938.
    HOGENOMi HOG000084335.
    KOi K12658.
    OMAi WIRQALM.
    OrthoDBi EOG6SNDV3.

    Enzyme and pathway databases

    BioCyci BPSE272560:GJNI-3867-MONOMER.
    BRENDAi 5.1.1.8. 1031.

    Family and domain databases

    InterProi IPR008794. Pro_racemase_fam.
    [Graphical view ]
    Pfami PF05544. Pro_racemase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF029792. Pro_racemase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
      Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
      PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K96243.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K96243.

    Entry informationi

    Entry nameiHYPRE_BURPS
    AccessioniPrimary (citable) accession number: Q63NG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3