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Q63NG7

- HYPRE_BURPS

UniProt

Q63NG7 - HYPRE_BURPS

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Protein

4-hydroxyproline epimerase

Gene

BPSS0332

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases.

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=14.4 mM for 4-hydroxy-L-proline1 Publication
  2. KM=17.1 mM for 4-hydroxy-D-proline1 Publication

Vmax=2.1 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)1 Publication

Vmax=2.4 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Proton acceptorBy similarity
Active sitei236 – 2361Proton donorBy similarity

GO - Molecular functioni

  1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
  2. proline racemase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-3867-MONOMER.
BRENDAi5.1.1.8. 1031.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyproline epimerase (EC:5.1.1.8)
Alternative name(s):
Hydroxyproline-2-epimerase
Short name:
BpHyPRE
Gene namesi
Ordered Locus Names:BPSS0332
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000000605: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3103104-hydroxyproline epimerasePRO_0000354034Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272560.BPSS0332.

Structurei

3D structure databases

ProteinModelPortaliQ63NG7.
SMRiQ63NG7. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084335.
KOiK12658.
OMAiWIRQALM.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63NG7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHIHIIDSH TGGEPTRVVV SGFPALGGGT MAERLAVLAR EHDRYRAACI
60 70 80 90 100
LEPRGSDVLV GALLCEPVSA GAAAGVIFFN NAGYLGMCGH GTIGLVRTLH
110 120 130 140 150
HMGRIGPGVH RIETPVGDVE ATLHDDLSVS VRNVLAYRHA KDVVVDVPGH
160 170 180 190 200
GAVTGDVAWG GNWFFLVSDH GQRVAGENVA ALAAYASAVR AALERAGVTG
210 220 230 240 250
RDGAPIDHIE LFADDPEYDS RSFVLCPGHA YDRSPCGTGT SAKLACLAAD
260 270 280 290 300
GKLAAGVTWR QASVIGSVFS ASYAAAEGGV VPTIRGSAHL SAEATLVIED
310
DDPFGWGIAS
Length:310
Mass (Da):32,118
Last modified:October 25, 2004 - v1
Checksum:i4315A2A48443A002
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495345 Genomic DNA. Translation: ABS82397.1.
BX571966 Genomic DNA. Translation: CAH37780.1.
RefSeqiWP_004523447.1. NC_006351.1.
YP_110352.1. NC_006351.1.

Genome annotation databases

EnsemblBacteriaiCAH37780; CAH37780; BPSS0332.
GeneIDi3097013.
KEGGibps:BPSS0332.
PATRICi19268294. VBIBurPse99623_4324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495345 Genomic DNA. Translation: ABS82397.1 .
BX571966 Genomic DNA. Translation: CAH37780.1 .
RefSeqi WP_004523447.1. NC_006351.1.
YP_110352.1. NC_006351.1.

3D structure databases

ProteinModelPortali Q63NG7.
SMRi Q63NG7. Positions 1-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272560.BPSS0332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH37780 ; CAH37780 ; BPSS0332 .
GeneIDi 3097013.
KEGGi bps:BPSS0332.
PATRICi 19268294. VBIBurPse99623_4324.

Phylogenomic databases

eggNOGi COG3938.
HOGENOMi HOG000084335.
KOi K12658.
OMAi WIRQALM.
OrthoDBi EOG6SNDV3.

Enzyme and pathway databases

BioCyci BPSE272560:GJNI-3867-MONOMER.
BRENDAi 5.1.1.8. 1031.

Family and domain databases

InterProi IPR008794. Pro_racemase_fam.
[Graphical view ]
Pfami PF05544. Pro_racemase. 1 hit.
[Graphical view ]
PIRSFi PIRSF029792. Pro_racemase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K96243.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiHYPRE_BURPS
AccessioniPrimary (citable) accession number: Q63NG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: October 25, 2004
Last modified: October 1, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3