Q63NG7 (HYPRE_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyproline epimerase
EC=5.1.1.8

Alternative name(s):
Hydroxyproline-2-epimerase
Short name=BpHyPRE

Gene names

Ordered Locus Names:

BPSS0332

Organism

Burkholderia pseudomallei (strain K96243) [Reference proteome] [HAMAP]

Taxonomic identifier

272560 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia pseudomallei

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases.
Catalytic activity	Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline. Ref.1
Enzyme regulation	Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM. Ref.1
Subunit structure	Homodimer By similarity.
Miscellaneous	This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.
Sequence similarities	Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.
Biophysicochemical properties	Kinetic parameters: KM=14.4 mM for 4-hydroxy-L-proline Ref.1 KM=17.1 mM for 4-hydroxy-D-proline Vmax=2.1 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius) Vmax=2.4 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	4-hydroxyproline epimerase activity Inferred from electronic annotation. Source: EC proline racemase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	4-hydroxyproline epimerase		PRO_0000354034
Sites
	Active site	88	1	Proton acceptor By similarity
	Active site	236	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63NG7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 4315A2A48443A002	FASTA	310	32,118
	10 20 30 40 50 60 MKHIHIIDSH TGGEPTRVVV SGFPALGGGT MAERLAVLAR EHDRYRAACI LEPRGSDVLV 70 80 90 100 110 120 GALLCEPVSA GAAAGVIFFN NAGYLGMCGH GTIGLVRTLH HMGRIGPGVH RIETPVGDVE 130 140 150 160 170 180 ATLHDDLSVS VRNVLAYRHA KDVVVDVPGH GAVTGDVAWG GNWFFLVSDH GQRVAGENVA 190 200 210 220 230 240 ALAAYASAVR AALERAGVTG RDGAPIDHIE LFADDPEYDS RSFVLCPGHA YDRSPCGTGT 250 260 270 280 290 300 SAKLACLAAD GKLAAGVTWR QASVIGSVFS ASYAAAEGGV VPTIRGSAHL SAEATLVIED 310 DDPFGWGIAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens." Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P. PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K96243.
[2]	"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF495345 Genomic DNA. Translation: ABS82397.1. BX571966 Genomic DNA. Translation: CAH37780.1.
RefSeq	YP_110352.1. NC_006351.1.
3D structure databases
HSSP	HSSP built from PDB template 2AZP based on UniProtKB Q9I476.
ProteinModelPortal	Q63NG7.
SMR	Q63NG7. Positions 1-308.
ModBase	Search...
Protein-protein interaction databases
STRING	272560.BPSS0332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3097013.
KEGG	bps:BPSS0332.
PATRIC	19268294. VBIBurPse99623_4324.
Phylogenomic databases
eggNOG	COG3938.
HOGENOM	HOG000084335.
KO	K12658.
OMA	NWFFLVA.
ProtClustDB	PRK13970.
Enzyme and pathway databases
BioCyc	BPSE272560:GJNI-3867-MONOMER.
BRENDA	5.1.1.8. 1031.
Family and domain databases
InterPro	IPR008794. Pro_racemase. [Graphical view]
Pfam	PF05544. Pro_racemase. 1 hit. [Graphical view]
PIRSF	PIRSF029792. Pro_racemase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HYPRE_BURPS

Accession

Primary (citable) accession number: Q63NG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program