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Q63KP6

- SYI2_BURPS

UniProt

Q63KP6 - SYI2_BURPS

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Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei598 – 5981Aminoacyl-adenylateUniRule annotation
Binding sitei642 – 6421ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-4881-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 2UniRule annotation
Short name:
IleRS 2UniRule annotation
Gene namesi
Name:ileS2UniRule annotation
Ordered Locus Names:BPSS1315
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000000605: Chromosome 2

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Isoleucine--tRNA ligase 2PRO_0000098371Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272560.BPSS1315.

Structurei

3D structure databases

ProteinModelPortaliQ63KP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi639 – 6435"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiARYDFLA.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63KP6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEYKETLNL LQTPFPMKGD LPRREPALVE RWAAQRVYAR MRAAAAGRPP
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAV NKVLKDIVLK SRFAAGYDAQ WIPGWDCHGM
110 120 130 140 150
PIEHRIEQLH GRGLPPEAVQ RLCREYAFEQ TERQRRDFLR LGLLGDWPHA
160 170 180 190 200
FRTMDFRTEA NELRFLERIR ARGLLYRGQK PVNWCVDCQS ALAEAELEYA
210 220 230 240 250
RKTSVAIHAG LRVRDPVDFA SRFRRRPVLD KPAMLVVWTT TPWTIPGNAA
260 270 280 290 300
AGVRADAPYG LYDTPGALIV VAQPLADALF ASLGVDHALC ALARGRELVG
310 320 330 340 350
LALGQPFFAG RDVPVVEAEF VTLDAGTGIV HLAPAHGAED AELCRRLGIA
360 370 380 390 400
GENVVDGAGR FAADLPEIGG LPLADGIARI VAKLRADGTL VREAAFEHAY
410 420 430 440 450
PHCWRHKTPI LFRSTPQWFI GMDIECEQGE ADPGRADVTE EAGATGEARK
460 470 480 490 500
VGKAEEAEEA GPVKTLRASA RDAIADVPFY PPSARQRMEA MIDGRPDWCV
510 520 530 540 550
SRQRTWGVPL PYFVRRDDRS LHPRSARLVE AVAARVERDG IAAWSRLRPA
560 570 580 590 600
ELGVDENAYE KLSDTLDVWF DSGSIHATVY RDAARADTGG YPADLYLEGA
610 620 630 640 650
DQHRGWFGAS LMTGCAADGR APFRAILTHG FVVDGAGRKM SKSLGNTVSP
660 670 680 690 700
QRIADTRGAD ILRLWIASTD YAAEMSISDE ILERVVETYR RMRNTLRFLL
710 720 730 740 750
QNVADFDPRD DAVPAGQLLD VDRYALARCR EFVDACRSAY ARYDFLAVTR
760 770 780 790 800
LAHGYCAEEL GGFYLDALKD RLYASVADGV ERRAAQTALH SVLANLLISI
810 820 830 840 850
APILSFTAEE AWTVFAGDER DSVFLHTWDE HAPPPDDAAL ARWAHVRALR
860 870 880 890 900
PHVTKALEEA RGAALIGRSS EAELVVRAPR DVLDALAPLH GELAAVFIVA
910 920 930 940 950
GVTLETADQI AVAVARTPLA RCERCWRHEP SVAAHASGDA LCARCRHALS
960
RRARAERSEP RAAVGSR
Length:967
Mass (Da):106,516
Last modified:October 25, 2004 - v1
Checksum:i91B4CC91BDD79B7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38786.1.
RefSeqiYP_111325.1. NC_006351.1.

Genome annotation databases

EnsemblBacteriaiCAH38786; CAH38786; BPSS1315.
GeneIDi3094966.
KEGGibps:BPSS1315.
PATRICi19270716. VBIBurPse99623_5528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38786.1 .
RefSeqi YP_111325.1. NC_006351.1.

3D structure databases

ProteinModelPortali Q63KP6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272560.BPSS1315.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH38786 ; CAH38786 ; BPSS1315 .
GeneIDi 3094966.
KEGGi bps:BPSS1315.
PATRICi 19270716. VBIBurPse99623_5528.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ARYDFLA.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BPSE272560:GJNI-4881-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiSYI2_BURPS
AccessioniPrimary (citable) accession number: Q63KP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3