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Q63KP6

- SYI2_BURPS

UniProt

Q63KP6 - SYI2_BURPS

Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei598 – 5981Aminoacyl-adenylateUniRule annotation
    Binding sitei642 – 6421ATPUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi942 – 9421ZincUniRule annotation
    Metal bindingi945 – 9451ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBPSE272560:GJNI-4881-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetase 2UniRule annotation
    Short name:
    IleRS 2UniRule annotation
    Gene namesi
    Name:ileS2UniRule annotation
    Ordered Locus Names:BPSS1315
    OrganismiBurkholderia pseudomallei (strain K96243)
    Taxonomic identifieri272560 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000000605: Chromosome 2

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Isoleucine--tRNA ligase 2PRO_0000098371Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272560.BPSS1315.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63KP6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi639 – 6435"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiARYDFLA.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63KP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEYKETLNL LQTPFPMKGD LPRREPALVE RWAAQRVYAR MRAAAAGRPP    50
    FVLHDGPPYA NGDIHIGHAV NKVLKDIVLK SRFAAGYDAQ WIPGWDCHGM 100
    PIEHRIEQLH GRGLPPEAVQ RLCREYAFEQ TERQRRDFLR LGLLGDWPHA 150
    FRTMDFRTEA NELRFLERIR ARGLLYRGQK PVNWCVDCQS ALAEAELEYA 200
    RKTSVAIHAG LRVRDPVDFA SRFRRRPVLD KPAMLVVWTT TPWTIPGNAA 250
    AGVRADAPYG LYDTPGALIV VAQPLADALF ASLGVDHALC ALARGRELVG 300
    LALGQPFFAG RDVPVVEAEF VTLDAGTGIV HLAPAHGAED AELCRRLGIA 350
    GENVVDGAGR FAADLPEIGG LPLADGIARI VAKLRADGTL VREAAFEHAY 400
    PHCWRHKTPI LFRSTPQWFI GMDIECEQGE ADPGRADVTE EAGATGEARK 450
    VGKAEEAEEA GPVKTLRASA RDAIADVPFY PPSARQRMEA MIDGRPDWCV 500
    SRQRTWGVPL PYFVRRDDRS LHPRSARLVE AVAARVERDG IAAWSRLRPA 550
    ELGVDENAYE KLSDTLDVWF DSGSIHATVY RDAARADTGG YPADLYLEGA 600
    DQHRGWFGAS LMTGCAADGR APFRAILTHG FVVDGAGRKM SKSLGNTVSP 650
    QRIADTRGAD ILRLWIASTD YAAEMSISDE ILERVVETYR RMRNTLRFLL 700
    QNVADFDPRD DAVPAGQLLD VDRYALARCR EFVDACRSAY ARYDFLAVTR 750
    LAHGYCAEEL GGFYLDALKD RLYASVADGV ERRAAQTALH SVLANLLISI 800
    APILSFTAEE AWTVFAGDER DSVFLHTWDE HAPPPDDAAL ARWAHVRALR 850
    PHVTKALEEA RGAALIGRSS EAELVVRAPR DVLDALAPLH GELAAVFIVA 900
    GVTLETADQI AVAVARTPLA RCERCWRHEP SVAAHASGDA LCARCRHALS 950
    RRARAERSEP RAAVGSR 967
    Length:967
    Mass (Da):106,516
    Last modified:October 25, 2004 - v1
    Checksum:i91B4CC91BDD79B7E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571966 Genomic DNA. Translation: CAH38786.1.
    RefSeqiWP_011205587.1. NC_006351.1.
    YP_111325.1. NC_006351.1.

    Genome annotation databases

    EnsemblBacteriaiCAH38786; CAH38786; BPSS1315.
    GeneIDi3094966.
    KEGGibps:BPSS1315.
    PATRICi19270716. VBIBurPse99623_5528.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571966 Genomic DNA. Translation: CAH38786.1 .
    RefSeqi WP_011205587.1. NC_006351.1.
    YP_111325.1. NC_006351.1.

    3D structure databases

    ProteinModelPortali Q63KP6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272560.BPSS1315.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH38786 ; CAH38786 ; BPSS1315 .
    GeneIDi 3094966.
    KEGGi bps:BPSS1315.
    PATRICi 19270716. VBIBurPse99623_5528.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ARYDFLA.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BPSE272560:GJNI-4881-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K96243.

    Entry informationi

    Entry nameiSYI2_BURPS
    AccessioniPrimary (citable) accession number: Q63KP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3