Q63KK7 (BETA_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline dehydrogenase
Short name=CDH
Short name=CHD
EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	BPSS1355

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: InterPro
Molecular function	choline dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase HAMAP MF_00750		PRO_0000258919
Regions
	Nucleotide binding	7 – 36	30	FAD Potential
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63KK7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 6990A10A40FD48C0	FASTA	565	62,703
	10 20 30 40 50 60 MTTREFDYII CGAGSAGNVL ATRLTEDPGV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMNHRRM ECGRGKGLGG SSLINGMCYI RGNALDYDNW ATHKALEDWA 130 140 150 160 170 180 YLDCLPYFRK AETRDVGPND YHGGDGPVSV TTSKPGVNPL FEAMVEAGVQ AGYPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTPRGRR ASTARGYLDQ ARARPNLEIV THALADRILF SGKRATGVTF 250 260 270 280 290 300 LHGSARVTAH ARREVLVCSG AIASPQLLQR SGVGPGEWLR ELDIPVVLDL PGVGRNLQDH 310 320 330 340 350 360 LEMYIQFECK EPVSLYPALK WWNQPKIGLE WMLNGTGLGA SNHFEAGGFI RTRDDDPWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIEM HGFQAHVGSM RSPSRGRVKL KSRDPHAHPS ILFNYMAEAL 430 440 450 460 470 480 DWREFRDAIR ATREIMRQPA LDRFRGRELN PGADLKSDNE LDTFVRARAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD NEGRVHGIDG LRVVDASIMP IITTGNLNAP TIMIAEKIAD RIRQHKPLER 550 560 SNAQYYVANG APARGGKPAR APAVV

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571966 Genomic DNA. Translation: CAH38826.1.
RefSeq	YP_111365.1. NC_006351.1.
3D structure databases
ProteinModelPortal	Q63KK7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3096018.
GenomeReviews	Gene locus BPSS1355 in contig BX571966_GR.
KEGG	bps:BPSS1355.
NMPDR	fig|272560.3.peg.1381.
PATRIC	19270810. VBIBurPse99623_5575.
Phylogenomic databases
HOGENOM	HBG734713.
OMA	RSTAPYY.
ProtClustDB	PRK02106.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSS1355-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
KO	K00108.
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. BetA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURPS

Accession

Primary (citable) accession number: Q63KK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program