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Protein

Guanine nucleotide exchange factor BopE

Gene

bopE

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Activator for both CDC42 and RAC1 by directly interacting with these Rho GTPases and acting as a guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, thus promoting bacterial entry into non-phagocytic cells.3 Publications

pH dependencei

Active in pH ranges of 3.5-10.5.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-5093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor BopE
Alternative name(s):
Effector protein BopE
Gene namesi
Name:bopE
Ordered Locus Names:BPSS1525
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000000605 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071R → E: Loss of GEF activity; when associated with P-216. 1 Publication
Mutagenesisi216 – 2161N → P: Loss of GEF activity; when associated with E-207. 1 Publication
Mutagenesisi224 – 2241N → P: Increased GEF activity; when associated with Q-230. 1 Publication
Mutagenesisi230 – 2301R → Q: Increased GEF activity; when associated with P-224. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Guanine nucleotide exchange factor BopEPRO_0000344030Add
BLAST

Interactioni

Subunit structurei

Monomer (Probable). Interacts with human CDC42.Curated2 Publications

Protein-protein interaction databases

STRINGi272560.BPSS1525.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni79 – 835Combined sources
Helixi84 – 9916Combined sources
Helixi101 – 1066Combined sources
Turni107 – 1093Combined sources
Helixi110 – 13223Combined sources
Helixi134 – 1363Combined sources
Beta strandi137 – 1415Combined sources
Helixi142 – 15615Combined sources
Beta strandi161 – 1633Combined sources
Beta strandi166 – 1683Combined sources
Helixi178 – 18811Combined sources
Helixi192 – 1954Combined sources
Helixi197 – 1993Combined sources
Helixi204 – 21613Combined sources
Helixi221 – 25131Combined sources
Beta strandi253 – 2586Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JOKNMR-A78-261[»]
2JOLNMR-A78-261[»]
ProteinModelPortaliQ63K41.
SMRiQ63K41. Positions 78-261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63K41.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000263487.
KOiK13741.
OMAiRQAIRHF.
OrthoDBiEOG6W71ZJ.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
[Graphical view]
PfamiPF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63K41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYNPRIGGF THVKQASFDV HVKRGEAQPR TSFAQQIKRI FSKIGETLGQ
60 70 80 90 100
LFRHRAPDSA PGRVRLQGVR YVGSYRPTGD AKQAIRHFVD EAVKQVAHAR
110 120 130 140 150
TPEIRQDAEF GRQVYEATLC AIFSEAKDRF CMDPATRAGN VRPAFIEALG
160 170 180 190 200
DAARATGLPG ADKQGVFTPS GAGTNPLYTE IRLRADTLMG AELAARPEYR
210 220 230 240 250
ELQPYARQQA IDLVANALPA ERSNTLVEFR QTVQTLEATY RRAAQDASRD
260
EKGATNAADG A
Length:261
Mass (Da):28,705
Last modified:October 25, 2004 - v1
Checksum:iA44EB3AB9CBFA621
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38998.1.
RefSeqiWP_004528812.1. NZ_CP009537.1.
YP_111531.1. NC_006351.1.

Genome annotation databases

EnsemblBacteriaiCAH38998; CAH38998; BPSS1525.
GeneIDi3096393.
KEGGibps:BPSS1525.
PATRICi19271222. VBIBurPse99623_5781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38998.1.
RefSeqiWP_004528812.1. NZ_CP009537.1.
YP_111531.1. NC_006351.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JOKNMR-A78-261[»]
2JOLNMR-A78-261[»]
ProteinModelPortaliQ63K41.
SMRiQ63K41. Positions 78-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSS1525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH38998; CAH38998; BPSS1525.
GeneIDi3096393.
KEGGibps:BPSS1525.
PATRICi19271222. VBIBurPse99623_5781.

Phylogenomic databases

HOGENOMiHOG000263487.
KOiK13741.
OMAiRQAIRHF.
OrthoDBiEOG6W71ZJ.

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-5093-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ63K41.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
[Graphical view]
PfamiPF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.
  2. "An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen."
    Stevens M.P., Wood M.W., Taylor L.A., Monaghan P., Hawes P., Jones P.W., Wallis T.S., Galyov E.E.
    Mol. Microbiol. 46:649-659(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN INVASION OF HOST CELLS.
    Strain: 10276.
  3. "A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity."
    Stevens M.P., Friebel A., Taylor L.A., Wood M.W., Brown P.J., Hardt W.-D., Galyov E.E.
    J. Bacteriol. 185:4992-4996(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN INVASION OF HOST CELLS, SUBCELLULAR LOCATION.
    Strain: 576.
  4. "Biophysical characterization of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei."
    Upadhyay A., Williams C., Gill A.C., Philippe D.L., Davis K., Taylor L.A., Stevens M.P., Galyov E.E., Bagby S.
    Biochim. Biophys. Acta 1698:111-119(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: 576.
  5. "Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis."
    Stevens M.P., Haque A., Atkins T., Hill J., Wood M.W., Easton A., Nelson M., Underwood-Fowler C., Titball R.W., Bancroft G.J., Galyov E.E.
    Microbiology 150:2669-2676(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN VIRULENCE.
    Strain: 576.
  6. "A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins."
    Haque A., Chu K., Easton A., Stevens M.P., Galyov E.E., Atkins T., Titball R., Bancroft G.J.
    J. Infect. Dis. 194:1241-1248(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMMUNOGENICITY.
    Strain: 576.
  7. "The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42."
    Upadhyay A., Wu H.-L., Williams C., Field T., Galyov E.E., van den Elsen J.M.H., Bagby S.
    Biochem. J. 411:485-493(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 78-261, MUTAGENESIS OF ARG-207; ASN-216; ASN-224 AND ARG-230, INTERACTION WITH HUMAN CDC42.

Entry informationi

Entry nameiBOPE_BURPS
AccessioniPrimary (citable) accession number: Q63K41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 25, 2004
Last modified: November 11, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Immunogenic protein that elicits T-cell-mediated immunity in mice.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.