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Protein

Guanine nucleotide exchange factor BopE

Gene

bopE

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Activator for both CDC42 and RAC1 by directly interacting with these Rho GTPases and acting as a guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, thus promoting bacterial entry into non-phagocytic cells.3 Publications

pH dependencei

Active in pH ranges of 3.5-10.5.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor BopE
Alternative name(s):
Effector protein BopE
Gene namesi
Name:bopE
Ordered Locus Names:BPSS1525
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000000605 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi207R → E: Loss of GEF activity; when associated with P-216. 1 Publication1
Mutagenesisi216N → P: Loss of GEF activity; when associated with E-207. 1 Publication1
Mutagenesisi224N → P: Increased GEF activity; when associated with Q-230. 1 Publication1
Mutagenesisi230R → Q: Increased GEF activity; when associated with P-224. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003440301 – 261Guanine nucleotide exchange factor BopEAdd BLAST261

Proteomic databases

PRIDEiQ63K41.

Interactioni

Subunit structurei

Monomer (Probable). Interacts with human CDC42.Curated2 Publications

Protein-protein interaction databases

STRINGi272560.BPSS1525.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni79 – 83Combined sources5
Helixi84 – 99Combined sources16
Helixi101 – 106Combined sources6
Turni107 – 109Combined sources3
Helixi110 – 132Combined sources23
Helixi134 – 136Combined sources3
Beta strandi137 – 141Combined sources5
Helixi142 – 156Combined sources15
Beta strandi161 – 163Combined sources3
Beta strandi166 – 168Combined sources3
Helixi178 – 188Combined sources11
Helixi192 – 195Combined sources4
Helixi197 – 199Combined sources3
Helixi204 – 216Combined sources13
Helixi221 – 251Combined sources31
Beta strandi253 – 258Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JOKNMR-A78-261[»]
2JOLNMR-A78-261[»]
ProteinModelPortaliQ63K41.
SMRiQ63K41.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63K41.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000263487.
KOiK13741.
OMAiRQAIRHF.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
[Graphical view]
PfamiPF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63K41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYNPRIGGF THVKQASFDV HVKRGEAQPR TSFAQQIKRI FSKIGETLGQ
60 70 80 90 100
LFRHRAPDSA PGRVRLQGVR YVGSYRPTGD AKQAIRHFVD EAVKQVAHAR
110 120 130 140 150
TPEIRQDAEF GRQVYEATLC AIFSEAKDRF CMDPATRAGN VRPAFIEALG
160 170 180 190 200
DAARATGLPG ADKQGVFTPS GAGTNPLYTE IRLRADTLMG AELAARPEYR
210 220 230 240 250
ELQPYARQQA IDLVANALPA ERSNTLVEFR QTVQTLEATY RRAAQDASRD
260
EKGATNAADG A
Length:261
Mass (Da):28,705
Last modified:October 25, 2004 - v1
Checksum:iA44EB3AB9CBFA621
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38998.1.
RefSeqiWP_004528812.1. NZ_CP009537.1.
YP_111531.1. NC_006351.1.

Genome annotation databases

EnsemblBacteriaiCAH38998; CAH38998; BPSS1525.
GeneIDi3096393.
KEGGibps:BPSS1525.
PATRICi19271222. VBIBurPse99623_5781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571966 Genomic DNA. Translation: CAH38998.1.
RefSeqiWP_004528812.1. NZ_CP009537.1.
YP_111531.1. NC_006351.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JOKNMR-A78-261[»]
2JOLNMR-A78-261[»]
ProteinModelPortaliQ63K41.
SMRiQ63K41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSS1525.

Proteomic databases

PRIDEiQ63K41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH38998; CAH38998; BPSS1525.
GeneIDi3096393.
KEGGibps:BPSS1525.
PATRICi19271222. VBIBurPse99623_5781.

Phylogenomic databases

HOGENOMiHOG000263487.
KOiK13741.
OMAiRQAIRHF.

Miscellaneous databases

EvolutionaryTraceiQ63K41.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
[Graphical view]
PfamiPF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBOPE_BURPS
AccessioniPrimary (citable) accession number: Q63K41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 25, 2004
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Immunogenic protein that elicits T-cell-mediated immunity in mice.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.