Q63JM0 (TRPA_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	BPSS1697

Organism

Burkholderia pseudomallei (strain K96243) [Reference proteome] [HAMAP]

Taxonomic identifier

272560 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia pseudomallei

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Tryptophan synthase alpha chain HAMAP-Rule MF_00131		PRO_0000098761
Sites
	Active site	49	1	Proton acceptor By similarity
	Active site	60	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63JM0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: AC4B9137254195CD	FASTA	271	27,978
	10 20 30 40 50 60 MSRIQNTFAA LAAQGRKGLI PFITAGDPDP AKTVELMHAL AEGGADVIEL GVPFSDPMAD 70 80 90 100 110 120 GPVIQRSSER ALAKGVTLHS VLDDVKRFRA RDQKTPVVLM GYANPIERMG ADAFAAAARD 130 140 150 160 170 180 AGVDGVLVVD YPPEESHDFA AKMRAAGIDP IFLLAPTSTD DRIAAVGQVA SGYVYYVSLK 190 200 210 220 230 240 GVTGAANLDV SSIAGKIPAI KSRVPLPVGV GFGIRDAATA RAVAEVADAV VIGSRLVQLL 250 260 270 EQAAPERAAA ELAGFVAELR VAIDGAAKPA A

References

[1]

"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571966 Genomic DNA. Translation: CAH39170.1.
RefSeq	YP_111702.1. NC_006351.1.
3D structure databases
ProteinModelPortal	Q63JM0.
ModBase	Search...
Protein-protein interaction databases
STRING	272560.BPSS1697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3096324.
KEGG	bps:BPSS1697.
PATRIC	19271656. VBIBurPse99623_5999.
Phylogenomic databases
eggNOG	COG0159.
HOGENOM	HOG000223815.
KO	K01695.
OMA	VFICPPN.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	BPSE272560:GJNI-5267-MONOMER.
UniPathway	UPA00035; UER00044.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00131. Trp_synth_alpha.
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. trpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_BURPS

Accession

Primary (citable) accession number: Q63JM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program