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Q63HQ0 (AP1AR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex-associated regulatory protein
Alternative name(s):
2c18
Adapter-related protein complex 1-associated regulatory protein
Gamma-1-adaptin brefeldin A resistance protein
Short name=GBAR
Short name=Gamma-BAR
Gamma-A1-adaptin and kinesin interactor
Short name=Gadkin
Gene names
Name:AP1AR
Synonyms:C4orf16
ORF Names:PRO0971
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex. Ref.7 Ref.10 Ref.12 Ref.13

Subunit structure

Interacts (via coiled-coil domain) with AP1G1 (via GAE domain). Interacts with KIF5B. Associates with the Arp2/3 complex. Ref.7 Ref.10 Ref.11

Subcellular location

Golgi apparatustrans-Golgi network. Late endosome. Early endosome. Note: Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery. Ref.7 Ref.11 Ref.12 Ref.13

Post-translational modification

Palmitoylated. Ref.11

Sequence caution

The sequence AAF71037.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentEndosome
Golgi apparatus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   PTMGlycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular protein localization

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell motility

Inferred from electronic annotation. Source: Ensembl

negative regulation of receptor recycling

Inferred from mutant phenotype Ref.10. Source: UniProtKB

negative regulation of substrate adhesion-dependent cell spreading

Inferred from mutant phenotype Ref.13. Source: MGI

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of Arp2/3 complex-mediated actin nucleation

Inferred from electronic annotation. Source: Ensembl

vesicle targeting, trans-Golgi to endosome

Inferred from direct assay Ref.10. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

early endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

late endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleolus

Inferred from direct assay. Source: HPA

transport vesicle

Inferred from direct assay. Source: LIFEdb

   Molecular_functionAP-1 adaptor complex binding

Inferred from direct assay Ref.10. Source: UniProtKB

kinesin binding

Inferred from direct assay Ref.10. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q63HQ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q63HQ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     94-126: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302AP-1 complex-associated regulatory protein
PRO_0000089432

Regions

Region78 – 13861Interaction with AP1G1
Region199 – 21517Sufficient for association with the Arp2/3 complex
Coiled coil80 – 13859 Potential
Compositional bias24 – 285Poly-Gly

Amino acid modifications

Modified residue2281Phosphothreonine Ref.8
Glycosylation2471N-linked (GlcNAc...) Ref.9

Natural variations

Alternative sequence94 – 12633Missing in isoform 2.
VSP_015339
Natural variant2971T → I.
Corresponds to variant rs34900583 [ dbSNP | Ensembl ].
VAR_050769

Experimental info

Mutagenesis4 – 52CC → SS: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 9-S.
Mutagenesis91C → S: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 4-S-S-5. Ref.11
Mutagenesis2101W → A: Loss of association with the Arp2/3 complex and endosomal colocalization. Abolishes interaction with KLC2, no effect on interaction with AP1G1. Ref.10 Ref.13
Mutagenesis2601W → L: Decreases interaction with AP1G1; when associated with L-264. Ref.11
Mutagenesis2641F → L: Decreases interaction with AP1G1; when associated with L-260. Ref.11
Sequence conflict891K → E in CAB66563. Ref.1
Sequence conflict2171N → D in BAG37704. Ref.2
Sequence conflict2431K → E in AAH09485. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 0827B5EC95DC133A

FASTA30234,280
        10         20         30         40         50         60 
MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS 

        70         80         90        100        110        120 
HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA 

       130        140        150        160        170        180 
KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL 

       190        200        210        220        230        240 
TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS 

       250        260        270        280        290        300 
NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ 


TR 

« Hide

Isoform 2 [UniParc].

Checksum: 63DCDB9D74E94F2B
Show »

FASTA26930,459

References

« Hide 'large scale' references
[1]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Endometrium and Fetal brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Pancreas.
[5]"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver."
Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-302.
Tissue: Fetal liver.
[6]"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[7]"Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking."
Neubrand V.E., Will R.D., Moebius W., Poustka A., Wiemann S., Schu P., Dotti C.G., Pepperkok R., Simpson J.C.
EMBO J. 24:1122-1133(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AP1G1.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-247.
Tissue: Leukemic T-cell.
[10]"Regulation of endosomal membrane traffic by a Gadkin/AP-1/kinesin KIF5 complex."
Schmidt M.R., Maritzen T., Kukhtina V., Higman V.A., Doglio L., Barak N.N., Strauss H., Oschkinat H., Dotti C.G., Haucke V.
Proc. Natl. Acad. Sci. U.S.A. 106:15344-15349(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AP1G1; KIF5B AND KLC2, MUTAGENESIS OF TRP-210.
[11]"A novel subtype of AP-1-binding motif within the palmitoylated trans-Golgi network/endosomal accessory protein Gadkin/gamma-BAR."
Maritzen T., Schmidt M.R., Kukhtina V., Higman V.A., Strauss H., Volkmer R., Oschkinat H., Dotti C.G., Haucke V.
J. Biol. Chem. 285:4074-4086(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION, SUBCELLULAR LOCATION, INTERACTION WITH AP1G1, MUTAGENESIS OF 4-CYS-CYS-5; CYS-9; TRP-260 AND PHE-264.
[12]"Role of AP1 and Gadkin in the traffic of secretory endo-lysosomes."
Laulagnier K., Schieber N.L., Maritzen T., Haucke V., Parton R.G., Gruenberg J.
Mol. Biol. Cell 22:2068-2082(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]"Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex."
Maritzen T., Zech T., Schmidt M.R., Krause E., Machesky L.M., Haucke V.
Proc. Natl. Acad. Sci. U.S.A. 109:10382-10387(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-210.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL136628 mRNA. Translation: CAB66563.1.
BX647702 mRNA. Translation: CAH56145.1.
AK315299 mRNA. Translation: BAG37704.1.
AC109347 Genomic DNA. Translation: AAY40962.1.
BC009485 mRNA. Translation: AAH09485.1.
AF116612 mRNA. Translation: AAF71037.1. Different initiation.
CCDSCCDS3696.1. [Q63HQ0-1]
CCDS47125.1. [Q63HQ0-2]
RefSeqNP_001121898.1. NM_001128426.1. [Q63HQ0-2]
NP_061039.3. NM_018569.4. [Q63HQ0-1]
UniGeneHs.435991.
Hs.606210.

3D structure databases

ProteinModelPortalQ63HQ0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120667. 2 interactions.
IntActQ63HQ0. 1 interaction.
MINTMINT-4724276.
STRING9606.ENSP00000274000.

PTM databases

PhosphoSiteQ63HQ0.

Polymorphism databases

DMDM73917693.

Proteomic databases

MaxQBQ63HQ0.
PaxDbQ63HQ0.
PRIDEQ63HQ0.

Protocols and materials databases

DNASU55435.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274000; ENSP00000274000; ENSG00000138660. [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660. [Q63HQ0-2]
GeneID55435.
KEGGhsa:55435.
UCSCuc003iaj.4. human. [Q63HQ0-1]
uc003iak.4. human. [Q63HQ0-2]

Organism-specific databases

CTD55435.
GeneCardsGC04P113153.
HGNCHGNC:28808. AP1AR.
HPAHPA035863.
MIM610851. gene.
neXtProtNX_Q63HQ0.
PharmGKBPA165663153.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG74642.
HOGENOMHOG000237335.
InParanoidQ63HQ0.
OMASNNGEYQ.
OrthoDBEOG7GXPC9.
PhylomeDBQ63HQ0.
TreeFamTF335676.

Gene expression databases

BgeeQ63HQ0.
CleanExHS_C4orf16.
GenevestigatorQ63HQ0.

Family and domain databases

ProtoNetSearch...

Other

GeneWikiAP1AR.
GenomeRNAi55435.
NextBio35468210.
PROQ63HQ0.
SOURCESearch...

Entry information

Entry nameAP1AR_HUMAN
AccessionPrimary (citable) accession number: Q63HQ0
Secondary accession number(s): B2RCV7 expand/collapse secondary AC list , Q96GG6, Q9H0V0, Q9P1L4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 25, 2004
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM