Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

AP-1 complex-associated regulatory protein

Gene

AP1AR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.4 Publications

GO - Molecular functioni

  • AP-1 adaptor complex binding Source: UniProtKB
  • kinesin binding Source: UniProtKB

GO - Biological processi

  • cellular protein localization Source: Ensembl
  • negative regulation of cell motility Source: Ensembl
  • negative regulation of receptor recycling Source: UniProtKB
  • negative regulation of substrate adhesion-dependent cell spreading Source: MGI
  • protein transport Source: UniProtKB-KW
  • regulation of Arp2/3 complex-mediated actin nucleation Source: Ensembl
  • vesicle targeting, trans-Golgi to endosome Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex-associated regulatory protein
Alternative name(s):
2c18
Adaptor-related protein complex 1-associated regulatory protein
Gamma-1-adaptin brefeldin A resistance protein
Short name:
GBAR
Short name:
Gamma-BAR
Gamma-A1-adaptin and kinesin interactor
Short name:
Gadkin
Gene namesi
Name:AP1AR
Synonyms:C4orf16
ORF Names:PRO0971
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:28808. AP1AR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 52CC → SS: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 9-S. 1 Publication
Mutagenesisi9 – 91C → S: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 4-S-S-5. 1 Publication
Mutagenesisi210 – 2101W → A: Loss of association with the Arp2/3 complex and endosomal colocalization. Abolishes interaction with KLC2, no effect on interaction with AP1G1. 2 Publications
Mutagenesisi260 – 2601W → L: Decreases interaction with AP1G1; when associated with L-264. 1 Publication
Mutagenesisi264 – 2641F → L: Decreases interaction with AP1G1; when associated with L-260. 1 Publication

Organism-specific databases

PharmGKBiPA165663153.

Polymorphism and mutation databases

BioMutaiAP1AR.
DMDMi73917693.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302AP-1 complex-associated regulatory proteinPRO_0000089432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphoserineCombined sources
Modified residuei226 – 2261PhosphoserineCombined sources
Modified residuei228 – 2281PhosphothreonineCombined sources
Glycosylationi247 – 2471N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Palmitoylated.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ63HQ0.
MaxQBiQ63HQ0.
PaxDbiQ63HQ0.
PeptideAtlasiQ63HQ0.
PRIDEiQ63HQ0.

PTM databases

iPTMnetiQ63HQ0.
PhosphoSiteiQ63HQ0.
SwissPalmiQ63HQ0.

Expressioni

Gene expression databases

BgeeiENSG00000138660.
CleanExiHS_C4orf16.
ExpressionAtlasiQ63HQ0. baseline and differential.
GenevisibleiQ63HQ0. HS.

Organism-specific databases

HPAiHPA035863.
HPA058115.

Interactioni

Subunit structurei

Interacts (via coiled-coil domain) with AP1G1 (via GAE domain). Interacts with KIF5B. Associates with the Arp2/3 complex.3 Publications

GO - Molecular functioni

  • AP-1 adaptor complex binding Source: UniProtKB
  • kinesin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120667. 2 interactions.
IntActiQ63HQ0. 2 interactions.
MINTiMINT-4724276.
STRINGi9606.ENSP00000274000.

Structurei

3D structure databases

ProteinModelPortaliQ63HQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 13861Interaction with AP1G1Add
BLAST
Regioni199 – 21517Sufficient for association with the Arp2/3 complexAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili80 – 13859Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 285Poly-Gly

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IK27. Eukaryota.
ENOG4111HXC. LUCA.
GeneTreeiENSGT00390000002219.
HOGENOMiHOG000237335.
InParanoidiQ63HQ0.
OMAiSNNGEYQ.
OrthoDBiEOG091G127J.
PhylomeDBiQ63HQ0.
TreeFamiTF335676.

Family and domain databases

InterProiIPR031483. AP1AR.
[Graphical view]
PfamiPF15745. AP1AR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63HQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE
60 70 80 90 100
SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE
110 120 130 140 150
KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP
160 170 180 190 200
EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN
210 220 230 240 250
DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS
260 270 280 290 300
ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ

TR
Length:302
Mass (Da):34,280
Last modified:October 25, 2004 - v1
Checksum:i0827B5EC95DC133A
GO
Isoform 2 (identifier: Q63HQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-126: Missing.

Show »
Length:269
Mass (Da):30,459
Checksum:i63DCDB9D74E94F2B
GO

Sequence cautioni

The sequence AAF71037 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891K → E in CAB66563 (PubMed:11230166).Curated
Sequence conflicti217 – 2171N → D in BAG37704 (PubMed:14702039).Curated
Sequence conflicti243 – 2431K → E in AAH09485 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti297 – 2971T → I.
Corresponds to variant rs34900583 [ dbSNP | Ensembl ].
VAR_050769

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei94 – 12633Missing in isoform 2. 1 PublicationVSP_015339Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA. Translation: CAB66563.1.
BX647702 mRNA. Translation: CAH56145.1.
AK315299 mRNA. Translation: BAG37704.1.
AC109347 Genomic DNA. Translation: AAY40962.1.
BC009485 mRNA. Translation: AAH09485.1.
AF116612 mRNA. Translation: AAF71037.1. Different initiation.
CCDSiCCDS3696.1. [Q63HQ0-1]
CCDS47125.1. [Q63HQ0-2]
RefSeqiNP_001121898.1. NM_001128426.2. [Q63HQ0-2]
NP_061039.3. NM_018569.5. [Q63HQ0-1]
UniGeneiHs.435991.
Hs.550015.
Hs.606210.

Genome annotation databases

EnsembliENST00000274000; ENSP00000274000; ENSG00000138660. [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660. [Q63HQ0-2]
GeneIDi55435.
KEGGihsa:55435.
UCSCiuc003iaj.6. human. [Q63HQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA. Translation: CAB66563.1.
BX647702 mRNA. Translation: CAH56145.1.
AK315299 mRNA. Translation: BAG37704.1.
AC109347 Genomic DNA. Translation: AAY40962.1.
BC009485 mRNA. Translation: AAH09485.1.
AF116612 mRNA. Translation: AAF71037.1. Different initiation.
CCDSiCCDS3696.1. [Q63HQ0-1]
CCDS47125.1. [Q63HQ0-2]
RefSeqiNP_001121898.1. NM_001128426.2. [Q63HQ0-2]
NP_061039.3. NM_018569.5. [Q63HQ0-1]
UniGeneiHs.435991.
Hs.550015.
Hs.606210.

3D structure databases

ProteinModelPortaliQ63HQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120667. 2 interactions.
IntActiQ63HQ0. 2 interactions.
MINTiMINT-4724276.
STRINGi9606.ENSP00000274000.

PTM databases

iPTMnetiQ63HQ0.
PhosphoSiteiQ63HQ0.
SwissPalmiQ63HQ0.

Polymorphism and mutation databases

BioMutaiAP1AR.
DMDMi73917693.

Proteomic databases

EPDiQ63HQ0.
MaxQBiQ63HQ0.
PaxDbiQ63HQ0.
PeptideAtlasiQ63HQ0.
PRIDEiQ63HQ0.

Protocols and materials databases

DNASUi55435.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274000; ENSP00000274000; ENSG00000138660. [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660. [Q63HQ0-2]
GeneIDi55435.
KEGGihsa:55435.
UCSCiuc003iaj.6. human. [Q63HQ0-1]

Organism-specific databases

CTDi55435.
GeneCardsiAP1AR.
HGNCiHGNC:28808. AP1AR.
HPAiHPA035863.
HPA058115.
MIMi610851. gene.
neXtProtiNX_Q63HQ0.
PharmGKBiPA165663153.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK27. Eukaryota.
ENOG4111HXC. LUCA.
GeneTreeiENSGT00390000002219.
HOGENOMiHOG000237335.
InParanoidiQ63HQ0.
OMAiSNNGEYQ.
OrthoDBiEOG091G127J.
PhylomeDBiQ63HQ0.
TreeFamiTF335676.

Miscellaneous databases

ChiTaRSiAP1AR. human.
GeneWikiiAP1AR.
GenomeRNAii55435.
PROiQ63HQ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138660.
CleanExiHS_C4orf16.
ExpressionAtlasiQ63HQ0. baseline and differential.
GenevisibleiQ63HQ0. HS.

Family and domain databases

InterProiIPR031483. AP1AR.
[Graphical view]
PfamiPF15745. AP1AR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP1AR_HUMAN
AccessioniPrimary (citable) accession number: Q63HQ0
Secondary accession number(s): B2RCV7
, Q96GG6, Q9H0V0, Q9P1L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.