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Q63HQ0

- AP1AR_HUMAN

UniProt

Q63HQ0 - AP1AR_HUMAN

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Protein

AP-1 complex-associated regulatory protein

Gene

AP1AR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.4 Publications

GO - Molecular functioni

  1. AP-1 adaptor complex binding Source: UniProtKB
  2. kinesin binding Source: UniProtKB

GO - Biological processi

  1. cellular protein localization Source: Ensembl
  2. negative regulation of cell motility Source: Ensembl
  3. negative regulation of receptor recycling Source: UniProtKB
  4. negative regulation of substrate adhesion-dependent cell spreading Source: MGI
  5. protein transport Source: UniProtKB-KW
  6. regulation of Arp2/3 complex-mediated actin nucleation Source: Ensembl
  7. vesicle targeting, trans-Golgi to endosome Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex-associated regulatory protein
Alternative name(s):
2c18
Adaptor-related protein complex 1-associated regulatory protein
Gamma-1-adaptin brefeldin A resistance protein
Short name:
GBAR
Short name:
Gamma-BAR
Gamma-A1-adaptin and kinesin interactor
Short name:
Gadkin
Gene namesi
Name:AP1AR
Synonyms:C4orf16
ORF Names:PRO0971
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:28808. AP1AR.

Subcellular locationi

Golgi apparatustrans-Golgi network. Late endosome. Early endosome
Note: Localizes to the juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle between the juxta-nuclear area and the cell periphery.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. endosome Source: UniProtKB-KW
  3. Golgi apparatus Source: HPA
  4. nucleolus Source: HPA
  5. transport vesicle Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 52CC → SS: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 9-S. 1 Publication
Mutagenesisi9 – 91C → S: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 4-S-S-5. 1 Publication
Mutagenesisi210 – 2101W → A: Loss of association with the Arp2/3 complex and endosomal colocalization. Abolishes interaction with KLC2, no effect on interaction with AP1G1. 2 Publications
Mutagenesisi260 – 2601W → L: Decreases interaction with AP1G1; when associated with L-264. 1 Publication
Mutagenesisi264 – 2641F → L: Decreases interaction with AP1G1; when associated with L-260. 1 Publication

Organism-specific databases

PharmGKBiPA165663153.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302AP-1 complex-associated regulatory proteinPRO_0000089432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281Phosphothreonine1 Publication
Glycosylationi247 – 2471N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Palmitoylated.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ63HQ0.
PaxDbiQ63HQ0.
PRIDEiQ63HQ0.

PTM databases

PhosphoSiteiQ63HQ0.

Expressioni

Gene expression databases

BgeeiQ63HQ0.
CleanExiHS_C4orf16.
GenevestigatoriQ63HQ0.

Organism-specific databases

HPAiHPA035863.

Interactioni

Subunit structurei

Interacts (via coiled-coil domain) with AP1G1 (via GAE domain). Interacts with KIF5B. Associates with the Arp2/3 complex.3 Publications

Protein-protein interaction databases

BioGridi120667. 2 interactions.
IntActiQ63HQ0. 1 interaction.
MINTiMINT-4724276.
STRINGi9606.ENSP00000274000.

Structurei

3D structure databases

ProteinModelPortaliQ63HQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 13861Interaction with AP1G1Add
BLAST
Regioni199 – 21517Sufficient for association with the Arp2/3 complexAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili80 – 13859Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 285Poly-Gly

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG74642.
GeneTreeiENSGT00390000002219.
HOGENOMiHOG000237335.
InParanoidiQ63HQ0.
OMAiSNNGEYQ.
OrthoDBiEOG7GXPC9.
PhylomeDBiQ63HQ0.
TreeFamiTF335676.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q63HQ0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE
60 70 80 90 100
SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE
110 120 130 140 150
KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP
160 170 180 190 200
EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN
210 220 230 240 250
DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS
260 270 280 290 300
ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ

TR
Length:302
Mass (Da):34,280
Last modified:October 25, 2004 - v1
Checksum:i0827B5EC95DC133A
GO
Isoform 2 (identifier: Q63HQ0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-126: Missing.

Show »
Length:269
Mass (Da):30,459
Checksum:i63DCDB9D74E94F2B
GO

Sequence cautioni

The sequence AAF71037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891K → E in CAB66563. (PubMed:11230166)Curated
Sequence conflicti217 – 2171N → D in BAG37704. (PubMed:14702039)Curated
Sequence conflicti243 – 2431K → E in AAH09485. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti297 – 2971T → I.
Corresponds to variant rs34900583 [ dbSNP | Ensembl ].
VAR_050769

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei94 – 12633Missing in isoform 2. 1 PublicationVSP_015339Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA. Translation: CAB66563.1.
BX647702 mRNA. Translation: CAH56145.1.
AK315299 mRNA. Translation: BAG37704.1.
AC109347 Genomic DNA. Translation: AAY40962.1.
BC009485 mRNA. Translation: AAH09485.1.
AF116612 mRNA. Translation: AAF71037.1. Different initiation.
CCDSiCCDS3696.1. [Q63HQ0-1]
CCDS47125.1. [Q63HQ0-2]
RefSeqiNP_001121898.1. NM_001128426.1. [Q63HQ0-2]
NP_061039.3. NM_018569.4. [Q63HQ0-1]
UniGeneiHs.435991.
Hs.606210.

Genome annotation databases

EnsembliENST00000274000; ENSP00000274000; ENSG00000138660. [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660. [Q63HQ0-2]
GeneIDi55435.
KEGGihsa:55435.
UCSCiuc003iaj.4. human. [Q63HQ0-1]
uc003iak.4. human. [Q63HQ0-2]

Polymorphism databases

DMDMi73917693.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA. Translation: CAB66563.1 .
BX647702 mRNA. Translation: CAH56145.1 .
AK315299 mRNA. Translation: BAG37704.1 .
AC109347 Genomic DNA. Translation: AAY40962.1 .
BC009485 mRNA. Translation: AAH09485.1 .
AF116612 mRNA. Translation: AAF71037.1 . Different initiation.
CCDSi CCDS3696.1. [Q63HQ0-1 ]
CCDS47125.1. [Q63HQ0-2 ]
RefSeqi NP_001121898.1. NM_001128426.1. [Q63HQ0-2 ]
NP_061039.3. NM_018569.4. [Q63HQ0-1 ]
UniGenei Hs.435991.
Hs.606210.

3D structure databases

ProteinModelPortali Q63HQ0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120667. 2 interactions.
IntActi Q63HQ0. 1 interaction.
MINTi MINT-4724276.
STRINGi 9606.ENSP00000274000.

PTM databases

PhosphoSitei Q63HQ0.

Polymorphism databases

DMDMi 73917693.

Proteomic databases

MaxQBi Q63HQ0.
PaxDbi Q63HQ0.
PRIDEi Q63HQ0.

Protocols and materials databases

DNASUi 55435.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000274000 ; ENSP00000274000 ; ENSG00000138660 . [Q63HQ0-1 ]
ENST00000309703 ; ENSP00000309023 ; ENSG00000138660 . [Q63HQ0-2 ]
GeneIDi 55435.
KEGGi hsa:55435.
UCSCi uc003iaj.4. human. [Q63HQ0-1 ]
uc003iak.4. human. [Q63HQ0-2 ]

Organism-specific databases

CTDi 55435.
GeneCardsi GC04P113153.
HGNCi HGNC:28808. AP1AR.
HPAi HPA035863.
MIMi 610851. gene.
neXtProti NX_Q63HQ0.
PharmGKBi PA165663153.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG74642.
GeneTreei ENSGT00390000002219.
HOGENOMi HOG000237335.
InParanoidi Q63HQ0.
OMAi SNNGEYQ.
OrthoDBi EOG7GXPC9.
PhylomeDBi Q63HQ0.
TreeFami TF335676.

Miscellaneous databases

ChiTaRSi AP1AR. human.
GeneWikii AP1AR.
GenomeRNAii 55435.
NextBioi 35468210.
PROi Q63HQ0.
SOURCEi Search...

Gene expression databases

Bgeei Q63HQ0.
CleanExi HS_C4orf16.
Genevestigatori Q63HQ0.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Endometrium and Fetal brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Pancreas.
  5. "Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver."
    Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-302.
    Tissue: Fetal liver.
  6. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
    Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
    Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. "Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking."
    Neubrand V.E., Will R.D., Moebius W., Poustka A., Wiemann S., Schu P., Dotti C.G., Pepperkok R., Simpson J.C.
    EMBO J. 24:1122-1133(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AP1G1.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-247.
    Tissue: Leukemic T-cell.
  10. Cited for: FUNCTION, INTERACTION WITH AP1G1; KIF5B AND KLC2, MUTAGENESIS OF TRP-210.
  11. "A novel subtype of AP-1-binding motif within the palmitoylated trans-Golgi network/endosomal accessory protein Gadkin/gamma-BAR."
    Maritzen T., Schmidt M.R., Kukhtina V., Higman V.A., Strauss H., Volkmer R., Oschkinat H., Dotti C.G., Haucke V.
    J. Biol. Chem. 285:4074-4086(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION, SUBCELLULAR LOCATION, INTERACTION WITH AP1G1, MUTAGENESIS OF 4-CYS-CYS-5; CYS-9; TRP-260 AND PHE-264.
  12. "Role of AP1 and Gadkin in the traffic of secretory endo-lysosomes."
    Laulagnier K., Schieber N.L., Maritzen T., Haucke V., Parton R.G., Gruenberg J.
    Mol. Biol. Cell 22:2068-2082(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex."
    Maritzen T., Zech T., Schmidt M.R., Krause E., Machesky L.M., Haucke V.
    Proc. Natl. Acad. Sci. U.S.A. 109:10382-10387(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-210.

Entry informationi

Entry nameiAP1AR_HUMAN
AccessioniPrimary (citable) accession number: Q63HQ0
Secondary accession number(s): B2RCV7
, Q96GG6, Q9H0V0, Q9P1L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 25, 2004
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3