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Q63HM1

- KFA_HUMAN

UniProt

Q63HM1 - KFA_HUMAN

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Protein

Kynurenine formamidase

Gene

AFMID

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641NucleophileUniRule annotation
Active sitei247 – 2471UniRule annotation
Active sitei279 – 2791UniRule annotation

GO - Molecular functioni

  1. arylformamidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. cellular nitrogen compound metabolic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
  4. tryptophan catabolic process Source: Reactome
  5. tryptophan catabolic process to kynurenine Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tryptophan catabolism

Enzyme and pathway databases

ReactomeiREACT_916. Tryptophan catabolism.
UniPathwayiUPA00333; UER00454.

Protein family/group databases

MEROPSiS09.977.

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:AFMIDUniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:20910. AFMID.

Subcellular locationi

Cytoplasmcytosol UniRule annotation. Nucleus UniRule annotation
Note: Predominantly cytosolic. Some fraction is nuclear.UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134958475.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Kynurenine formamidasePRO_0000248308Add
BLAST

Proteomic databases

MaxQBiQ63HM1.
PaxDbiQ63HM1.
PRIDEiQ63HM1.

PTM databases

PhosphoSiteiQ63HM1.

Expressioni

Gene expression databases

BgeeiQ63HM1.
CleanExiHS_AFMID.
ExpressionAtlasiQ63HM1. baseline and differential.
GenevestigatoriQ63HM1.

Organism-specific databases

HPAiHPA023861.
HPA026536.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi125914. 3 interactions.
STRINGi9606.ENSP00000328938.

Structurei

3D structure databases

ProteinModelPortaliQ63HM1.
SMRiQ63HM1. Positions 29-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi95 – 995HGGXW

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0657.
GeneTreeiENSGT00390000011093.
HOGENOMiHOG000260457.
HOVERGENiHBG100436.
InParanoidiQ63HM1.
KOiK01432.
OMAiMTMERLY.
PhylomeDBiQ63HM1.
TreeFamiTF315112.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_03014. KFase.
InterProiIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR027519. KFase_ver/fungi-typ.
[Graphical view]
PfamiPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q63HM1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI
60 70 80 90 100
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ
110 120 130 140 150
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK
160 170 180 190 200
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE
210 220 230 240 250
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE
260 270 280 290 300
FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT

IFQ
Length:303
Mass (Da):33,992
Last modified:September 22, 2009 - v2
Checksum:i6E329218366C027D
GO
Isoform 2 (identifier: Q63HM1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: Q → QVLPVQ

Note: No experimental confirmation available.

Show »
Length:308
Mass (Da):34,528
Checksum:i06640990D1E1B57C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti222 – 2221S → N in CAH56149. (PubMed:17974005)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei260 – 2601Q → QVLPVQ in isoform 2. 1 PublicationVSP_038002

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX648442 mRNA. Translation: CAH56149.1.
BC132824 mRNA. Translation: AAI32825.1.
CCDSiCCDS32750.2. [Q63HM1-1]
CCDS45801.1. [Q63HM1-2]
RefSeqiNP_001010982.2. NM_001010982.4. [Q63HM1-1]
NP_001138998.1. NM_001145526.2. [Q63HM1-2]
UniGeneiHs.558614.

Genome annotation databases

EnsembliENST00000327898; ENSP00000328938; ENSG00000183077. [Q63HM1-2]
ENST00000409257; ENSP00000386890; ENSG00000183077. [Q63HM1-1]
GeneIDi125061.
KEGGihsa:125061.
UCSCiuc002juz.3. human. [Q63HM1-2]
uc002jva.3. human. [Q63HM1-1]

Polymorphism databases

DMDMi259016175.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX648442 mRNA. Translation: CAH56149.1 .
BC132824 mRNA. Translation: AAI32825.1 .
CCDSi CCDS32750.2. [Q63HM1-1 ]
CCDS45801.1. [Q63HM1-2 ]
RefSeqi NP_001010982.2. NM_001010982.4. [Q63HM1-1 ]
NP_001138998.1. NM_001145526.2. [Q63HM1-2 ]
UniGenei Hs.558614.

3D structure databases

ProteinModelPortali Q63HM1.
SMRi Q63HM1. Positions 29-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125914. 3 interactions.
STRINGi 9606.ENSP00000328938.

Protein family/group databases

MEROPSi S09.977.

PTM databases

PhosphoSitei Q63HM1.

Polymorphism databases

DMDMi 259016175.

Proteomic databases

MaxQBi Q63HM1.
PaxDbi Q63HM1.
PRIDEi Q63HM1.

Protocols and materials databases

DNASUi 125061.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000327898 ; ENSP00000328938 ; ENSG00000183077 . [Q63HM1-2 ]
ENST00000409257 ; ENSP00000386890 ; ENSG00000183077 . [Q63HM1-1 ]
GeneIDi 125061.
KEGGi hsa:125061.
UCSCi uc002juz.3. human. [Q63HM1-2 ]
uc002jva.3. human. [Q63HM1-1 ]

Organism-specific databases

CTDi 125061.
GeneCardsi GC17P076183.
HGNCi HGNC:20910. AFMID.
HPAi HPA023861.
HPA026536.
neXtProti NX_Q63HM1.
PharmGKBi PA134958475.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0657.
GeneTreei ENSGT00390000011093.
HOGENOMi HOG000260457.
HOVERGENi HBG100436.
InParanoidi Q63HM1.
KOi K01432.
OMAi MTMERLY.
PhylomeDBi Q63HM1.
TreeFami TF315112.

Enzyme and pathway databases

UniPathwayi UPA00333 ; UER00454 .
Reactomei REACT_916. Tryptophan catabolism.

Miscellaneous databases

ChiTaRSi AFMID. human.
GenomeRNAii 125061.
NextBioi 81477.
PROi Q63HM1.

Gene expression databases

Bgeei Q63HM1.
CleanExi HS_AFMID.
ExpressionAtlasi Q63HM1. baseline and differential.
Genevestigatori Q63HM1.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_03014. KFase.
InterProi IPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR027519. KFase_ver/fungi-typ.
[Graphical view ]
Pfami PF07859. Abhydrolase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Salivary gland.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiKFA_HUMAN
AccessioniPrimary (citable) accession number: Q63HM1
Secondary accession number(s): A2RUB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 22, 2009
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3