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Q63HM1 (KFA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kynurenine formamidase

Short name=KFA
Short name=KFase
EC=3.5.1.9
Alternative name(s):
Arylformamidase
N-formylkynurenine formamidase
Short name=FKF
Gene names
Name:AFMID
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites By similarity. HAMAP-Rule MF_03014

Catalytic activity

N-formyl-L-kynurenine + H2O = formate + L-kynurenine. HAMAP-Rule MF_03014

Pathway

Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2. HAMAP-Rule MF_03014

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_03014

Subcellular location

Cytoplasmcytosol By similarity. Nucleus By similarity. Note: Predominantly cytosolic. Some fraction is nuclear By similarity. HAMAP-Rule MF_03014

Domain

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage By similarity. HAMAP-Rule MF_03014

Sequence similarities

Belongs to the kynurenine formamidase family.

Ontologies

Keywords
   Biological processTryptophan catabolism
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process'de novo' NAD biosynthetic process from tryptophan

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cellular nitrogen compound metabolic process

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

tryptophan catabolic process

Traceable author statement. Source: Reactome

tryptophan catabolic process to kynurenine

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionarylformamidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q63HM1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q63HM1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: Q → QVLPVQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Kynurenine formamidase HAMAP-Rule MF_03014
PRO_0000248308

Regions

Motif95 – 995HGGXW HAMAP-Rule MF_03014

Sites

Active site1641Nucleophile By similarity
Active site2471 By similarity
Active site2791 By similarity

Natural variations

Alternative sequence2601Q → QVLPVQ in isoform 2.
VSP_038002

Experimental info

Sequence conflict2221S → N in CAH56149. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 6E329218366C027D

FASTA30333,992
        10         20         30         40         50         60 
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI EATTRARATR 

        70         80         90        100        110        120 
KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ SGSKDESAFM VHPLTAQGVA 

       130        140        150        160        170        180 
VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK RYPSNKGIYL CGHSAGAHLA AMMLLADWTK 

       190        200        210        220        230        240 
HGVTPNLRGF FLVSGVFDLE PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL 

       250        260        270        280        290        300 
VVVGQFDSPE FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT 


IFQ 

« Hide

Isoform 2 [UniParc].

Checksum: 06640990D1E1B57C
Show »

FASTA30834,528

References

[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Salivary gland.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX648442 mRNA. Translation: CAH56149.1.
BC132824 mRNA. Translation: AAI32825.1.
RefSeqNP_001010982.2. NM_001010982.4.
NP_001138998.1. NM_001145526.2.
UniGeneHs.558614.

3D structure databases

ProteinModelPortalQ63HM1.
SMRQ63HM1. Positions 29-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125914. 1 interaction.
STRING9606.ENSP00000328938.

Protein family/group databases

MEROPSS09.977.

PTM databases

PhosphoSiteQ63HM1.

Polymorphism databases

DMDM259016175.

Proteomic databases

PaxDbQ63HM1.
PRIDEQ63HM1.

Protocols and materials databases

DNASU125061.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327898; ENSP00000328938; ENSG00000183077. [Q63HM1-2]
ENST00000409257; ENSP00000386890; ENSG00000183077. [Q63HM1-1]
GeneID125061.
KEGGhsa:125061.
UCSCuc002juz.3. human. [Q63HM1-2]
uc002jva.3. human. [Q63HM1-1]

Organism-specific databases

CTD125061.
GeneCardsGC17P076183.
HGNCHGNC:20910. AFMID.
HPAHPA023861.
HPA026536.
neXtProtNX_Q63HM1.
PharmGKBPA134958475.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0657.
HOGENOMHOG000260457.
HOVERGENHBG100436.
InParanoidQ63HM1.
KOK01432.
OMAKEESGFM.
PhylomeDBQ63HM1.
TreeFamTF315112.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
UniPathwayUPA00333; UER00454.

Gene expression databases

ArrayExpressQ63HM1.
BgeeQ63HM1.
CleanExHS_AFMID.
GenevestigatorQ63HM1.

Family and domain databases

HAMAPMF_03014. KFase.
InterProIPR013094. AB_hydrolase_3.
IPR027519. KFase.
[Graphical view]
PfamPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAFMID. human.
GenomeRNAi125061.
NextBio81477.
PROQ63HM1.

Entry information

Entry nameKFA_HUMAN
AccessionPrimary (citable) accession number: Q63HM1
Secondary accession number(s): A2RUB3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 22, 2009
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM