Q63HM1 (KFA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kynurenine formamidase
Short name=KFA
Short name=KFase
EC=3.5.1.9

Alternative name(s):
Arylformamidase
N-formylkynurenine formamidase
Short name=FKF

Gene names

Name:

AFMID

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites By similarity. HAMAP-Rule MF_03014
Catalytic activity	N-formyl-L-kynurenine + H2O = formate + L-kynurenine. HAMAP-Rule MF_03014
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2. HAMAP-Rule MF_03014
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity. Note: Predominantly cytosolic. Some fraction is nuclear By similarity.
Domain	The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage By similarity. HAMAP-Rule MF_03014
Sequence similarities	Belongs to the kynurenine formamidase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan catabolic process Traceable author statement. Source: Reactome tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	arylformamidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q63HM1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q63HM1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: Q → QVLPVQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Kynurenine formamidase HAMAP-Rule MF_03014		PRO_0000248308
Regions
	Motif	95 – 99	5	HGGXW HAMAP-Rule MF_03014
Sites
	Active site	164	1	Nucleophile By similarity
	Active site	247	1	By similarity
	Active site	279	1	By similarity
Natural variations
	Alternative sequence	260	1	Q → QVLPVQ in isoform 2.		VSP_038002
Experimental info
	Sequence conflict	222	1	S → N in CAH56149. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 6E329218366C027D	FASTA	303	33,992
	10 20 30 40 50 60 MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI EATTRARATR 70 80 90 100 110 120 KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ SGSKDESAFM VHPLTAQGVA 130 140 150 160 170 180 VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK RYPSNKGIYL CGHSAGAHLA AMMLLADWTK 190 200 210 220 230 240 HGVTPNLRGF FLVSGVFDLE PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL 250 260 270 280 290 300 VVVGQFDSPE FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT IFQ
	Isoform 2 [UniParc]. Checksum: 06640990D1E1B57C	FASTA	308	34,528
	10 20 30 40 50 60 MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI EATTRARATR 70 80 90 100 110 120 KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ SGSKDESAFM VHPLTAQGVA 130 140 150 160 170 180 VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK RYPSNKGIYL CGHSAGAHLA AMMLLADWTK 190 200 210 220 230 240 HGVTPNLRGF FLVSGVFDLE PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL 250 260 270 280 290 300 VVVGQFDSPE FHRQSWEFYQ VLPVQTLCQG EWKASFEELH DVDHFEIVEN LTQKDNVLTQ IILKTIFQ

References

[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Salivary gland.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX648442 mRNA. Translation: CAH56149.1. BC132824 mRNA. Translation: AAI32825.1.
IPI	IPI00470470. IPI00917771.
RefSeq	NP_001010982.2. NM_001010982.4. NP_001138998.1. NM_001145526.2.
UniGene	Hs.558614.
3D structure databases
ProteinModelPortal	Q63HM1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000328938.
Protein family/group databases
MEROPS	S09.977.
PTM databases
PhosphoSite	Q63HM1.
Polymorphism databases
DMDM	259016175.
Proteomic databases
PaxDb	Q63HM1.
PRIDE	Q63HM1.
Protocols and materials databases
DNASU	125061.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327898; ENSP00000328938; ENSG00000183077. ENST00000409257; ENSP00000386890; ENSG00000183077.
GeneID	125061.
KEGG	hsa:125061.
UCSC	uc002juz.3. human. uc002jva.3. human.
Organism-specific databases
CTD	125061.
GeneCards	GC17P076183.
HGNC	HGNC:20910. AFMID.
HPA	HPA023861. HPA026536.
neXtProt	NX_Q63HM1.
PharmGKB	PA134958475.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0657.
HOGENOM	HOG000260457.
HOVERGEN	HBG100436.
InParanoid	Q63HM1.
KO	K01432.
OMA	QYSHISG.
OrthoDB	EOG470THR.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00333; UER00454.
Gene expression databases
ArrayExpress	Q63HM1.
Bgee	Q63HM1.
CleanEx	HS_AFMID.
Genevestigator	Q63HM1.
Family and domain databases
HAMAP	MF_03014. KFase.
InterPro	IPR013094. AB_hydrolase_3. [Graphical view]
Pfam	PF07859. Abhydrolase_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AFMID. human.
GenomeRNAi	125061.
NextBio	81477.

Entry information

Entry name

KFA_HUMAN

Accession

Primary (citable) accession number: Q63HM1
Secondary accession number(s): A2RUB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 22, 2009
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.