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Q63HM1

- KFA_HUMAN

UniProt

Q63HM1 - KFA_HUMAN

Protein

Kynurenine formamidase

Gene

AFMID

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

    Catalytic activityi

    N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641NucleophileUniRule annotation
    Active sitei247 – 2471UniRule annotation
    Active sitei279 – 2791UniRule annotation

    GO - Molecular functioni

    1. arylformamidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
    2. cellular nitrogen compound metabolic process Source: Reactome
    3. small molecule metabolic process Source: Reactome
    4. tryptophan catabolic process Source: Reactome
    5. tryptophan catabolic process to kynurenine Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Tryptophan catabolism

    Enzyme and pathway databases

    ReactomeiREACT_916. Tryptophan catabolism.
    UniPathwayiUPA00333; UER00454.

    Protein family/group databases

    MEROPSiS09.977.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
    Short name:
    KFAUniRule annotation
    Short name:
    KFaseUniRule annotation
    Alternative name(s):
    ArylformamidaseUniRule annotation
    N-formylkynurenine formamidaseUniRule annotation
    Short name:
    FKFUniRule annotation
    Gene namesi
    Name:AFMIDUniRule annotation
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:20910. AFMID.

    Subcellular locationi

    Cytoplasmcytosol UniRule annotation. Nucleus UniRule annotation
    Note: Predominantly cytosolic. Some fraction is nuclear.UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134958475.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Kynurenine formamidasePRO_0000248308Add
    BLAST

    Proteomic databases

    MaxQBiQ63HM1.
    PaxDbiQ63HM1.
    PRIDEiQ63HM1.

    PTM databases

    PhosphoSiteiQ63HM1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ63HM1.
    BgeeiQ63HM1.
    CleanExiHS_AFMID.
    GenevestigatoriQ63HM1.

    Organism-specific databases

    HPAiHPA023861.
    HPA026536.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    BioGridi125914. 1 interaction.
    STRINGi9606.ENSP00000328938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63HM1.
    SMRiQ63HM1. Positions 29-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi95 – 995HGGXW

    Domaini

    The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

    Sequence similaritiesi

    Belongs to the kynurenine formamidase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0657.
    HOGENOMiHOG000260457.
    HOVERGENiHBG100436.
    InParanoidiQ63HM1.
    KOiK01432.
    OMAiMTMERLY.
    PhylomeDBiQ63HM1.
    TreeFamiTF315112.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_03014. KFase.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR027519. KFase_ver/fungi-typ.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q63HM1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI    50
    EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ 100
    SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK 150
    RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE 200
    PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE 250
    FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT 300
    IFQ 303
    Length:303
    Mass (Da):33,992
    Last modified:September 22, 2009 - v2
    Checksum:i6E329218366C027D
    GO
    Isoform 2 (identifier: Q63HM1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         260-260: Q → QVLPVQ

    Note: No experimental confirmation available.

    Show »
    Length:308
    Mass (Da):34,528
    Checksum:i06640990D1E1B57C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti222 – 2221S → N in CAH56149. (PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei260 – 2601Q → QVLPVQ in isoform 2. 1 PublicationVSP_038002

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX648442 mRNA. Translation: CAH56149.1.
    BC132824 mRNA. Translation: AAI32825.1.
    CCDSiCCDS32750.2. [Q63HM1-1]
    CCDS45801.1. [Q63HM1-2]
    RefSeqiNP_001010982.2. NM_001010982.4. [Q63HM1-1]
    NP_001138998.1. NM_001145526.2. [Q63HM1-2]
    UniGeneiHs.558614.

    Genome annotation databases

    EnsembliENST00000327898; ENSP00000328938; ENSG00000183077. [Q63HM1-2]
    ENST00000409257; ENSP00000386890; ENSG00000183077. [Q63HM1-1]
    GeneIDi125061.
    KEGGihsa:125061.
    UCSCiuc002juz.3. human. [Q63HM1-2]
    uc002jva.3. human. [Q63HM1-1]

    Polymorphism databases

    DMDMi259016175.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX648442 mRNA. Translation: CAH56149.1 .
    BC132824 mRNA. Translation: AAI32825.1 .
    CCDSi CCDS32750.2. [Q63HM1-1 ]
    CCDS45801.1. [Q63HM1-2 ]
    RefSeqi NP_001010982.2. NM_001010982.4. [Q63HM1-1 ]
    NP_001138998.1. NM_001145526.2. [Q63HM1-2 ]
    UniGenei Hs.558614.

    3D structure databases

    ProteinModelPortali Q63HM1.
    SMRi Q63HM1. Positions 29-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125914. 1 interaction.
    STRINGi 9606.ENSP00000328938.

    Protein family/group databases

    MEROPSi S09.977.

    PTM databases

    PhosphoSitei Q63HM1.

    Polymorphism databases

    DMDMi 259016175.

    Proteomic databases

    MaxQBi Q63HM1.
    PaxDbi Q63HM1.
    PRIDEi Q63HM1.

    Protocols and materials databases

    DNASUi 125061.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000327898 ; ENSP00000328938 ; ENSG00000183077 . [Q63HM1-2 ]
    ENST00000409257 ; ENSP00000386890 ; ENSG00000183077 . [Q63HM1-1 ]
    GeneIDi 125061.
    KEGGi hsa:125061.
    UCSCi uc002juz.3. human. [Q63HM1-2 ]
    uc002jva.3. human. [Q63HM1-1 ]

    Organism-specific databases

    CTDi 125061.
    GeneCardsi GC17P076183.
    HGNCi HGNC:20910. AFMID.
    HPAi HPA023861.
    HPA026536.
    neXtProti NX_Q63HM1.
    PharmGKBi PA134958475.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0657.
    HOGENOMi HOG000260457.
    HOVERGENi HBG100436.
    InParanoidi Q63HM1.
    KOi K01432.
    OMAi MTMERLY.
    PhylomeDBi Q63HM1.
    TreeFami TF315112.

    Enzyme and pathway databases

    UniPathwayi UPA00333 ; UER00454 .
    Reactomei REACT_916. Tryptophan catabolism.

    Miscellaneous databases

    ChiTaRSi AFMID. human.
    GenomeRNAii 125061.
    NextBioi 81477.
    PROi Q63HM1.

    Gene expression databases

    ArrayExpressi Q63HM1.
    Bgeei Q63HM1.
    CleanExi HS_AFMID.
    Genevestigatori Q63HM1.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    HAMAPi MF_03014. KFase.
    InterProi IPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR027519. KFase_ver/fungi-typ.
    [Graphical view ]
    Pfami PF07859. Abhydrolase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Salivary gland.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiKFA_HUMAN
    AccessioniPrimary (citable) accession number: Q63HM1
    Secondary accession number(s): A2RUB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3