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Q63HK5

- TSH3_HUMAN

UniProt

Q63HK5 - TSH3_HUMAN

Protein

Teashirt homolog 3

Gene

TSHZ3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri386 – 40419C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi891 – 96171Homeobox; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding Source: InterPro
    5. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. kidney morphogenesis Source: Ensembl
    3. kidney smooth muscle cell differentiation Source: Ensembl
    4. lung development Source: Ensembl
    5. metanephros development Source: Ensembl
    6. musculoskeletal movement Source: Ensembl
    7. negative regulation of transcription, DNA-templated Source: UniProtKB
    8. positive regulation of smooth muscle cell differentiation Source: Ensembl
    9. regulation of respiratory gaseous exchange by neurological system process Source: UniProtKB
    10. sensory perception of touch Source: Ensembl
    11. transcription, DNA-templated Source: UniProtKB-KW
    12. ureteric bud development Source: Ensembl
    13. ureteric peristalsis Source: Ensembl
    14. ureter smooth muscle cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Teashirt homolog 3
    Alternative name(s):
    Zinc finger protein 537
    Gene namesi
    Name:TSHZ3
    Synonyms:KIAA1474, TSH3, ZNF537
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:30700. TSHZ3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation. Cell projectiongrowth cone By similarity
    Note: Colocalizes with APBB1 in axonal growth cone By similarity. Colocalizes with APBB1 in the nucleus.By similarity

    GO - Cellular componenti

    1. growth cone Source: UniProtKB-SubCell
    2. mitochondrion Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi953 – 9553VKY → ATA: Does not inhibit interaction with APBB1. 1 Publication

    Organism-specific databases

    PharmGKBiPA134887020.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10811081Teashirt homolog 3PRO_0000047066Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei682 – 6821Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ63HK5.
    PaxDbiQ63HK5.
    PRIDEiQ63HK5.

    PTM databases

    PhosphoSiteiQ63HK5.

    Expressioni

    Tissue specificityi

    Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.2 Publications

    Developmental stagei

    Expressed in peri-urothelial cells of the proximal ureter and renal pelvis at 9 weeks of gestation.1 Publication

    Gene expression databases

    ArrayExpressiQ63HK5.
    BgeeiQ63HK5.
    CleanExiHS_TSHZ3.
    GenevestigatoriQ63HK5.

    Organism-specific databases

    HPAiHPA008834.

    Interactioni

    Subunit structurei

    Interacts (via homeobox domain) with APBB1 (via PID domain 1). Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3.1 Publication

    Protein-protein interaction databases

    BioGridi121663. 3 interactions.
    IntActiQ63HK5. 1 interaction.
    MINTiMINT-4725267.
    STRINGi9606.ENSP00000240587.

    Structurei

    Secondary structure

    1
    1081
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi213 – 2208
    Beta strandi222 – 2254
    Helixi226 – 23510
    Beta strandi278 – 2803
    Helixi287 – 29610
    Turni297 – 3015

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DMINMR-A202-303[»]
    ProteinModelPortaliQ63HK5.
    SMRiQ63HK5. Positions 200-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ63HK5.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili606 – 63025Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi142 – 16423Ser-richAdd
    BLAST
    Compositional biasi493 – 4964Poly-Glu

    Sequence similaritiesi

    Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri386 – 40419C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Homeobox, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG83709.
    HOGENOMiHOG000231480.
    HOVERGENiHBG079626.
    InParanoidiQ63HK5.
    KOiK09236.
    OMAiDPMSMLF.
    OrthoDBiEOG7NPFSF.
    PhylomeDBiQ63HK5.
    TreeFamiTF328447.

    Family and domain databases

    InterProiIPR001356. Homeobox_dom.
    IPR027008. Teashirt_fam.
    IPR026810. Tshz3.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR12487. PTHR12487. 1 hit.
    PTHR12487:SF5. PTHR12487:SF5. 1 hit.
    SMARTiSM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 5 hits.
    [Graphical view]
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63HK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRRKQQAPR RAAAYVSEEL KAAALVDEGL DPEEHTADGE PSAKYMCPEK     50
    ELARACPSYQ NSPAAEFSCH EMDSESHISE TSDRMADFES GSIKNEEETK 100
    EVTVPLEDTT VSDSLEQMKA VYNNFLSNSY WSNLNLNLHQ PSSEKNNGSS 150
    SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS QSRMLPEPSL FSTVQLYRQS 200
    SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR DDNHETDNNN 250
    PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY 300
    QKVPLKEPVT PVAAKIIPAT RKKASLELEL PSSPDSTGGT PKATISDTND 350
    ALQKNSNPYI TPNNRYGHQN GASYAWHFEA RKSQILKCME CGSSHDTLQE 400
    LTAHMMVTGH FIKVTNSAMK KGKPIVETPV TPTITTLLDE KVQSVPLAAT 450
    TFTSPSNTPA SISPKLNVEV KKEVDKEKAV TDEKPKQKDK PGEEEEKCDI 500
    SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG TPSWGGYPSI 550
    HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKNQT LVSPPSSQTS 600
    PMPKTNFHAM EELVKKVTEK VAKVEEKMKE PDGKLSPPKR ATPSPCSSEV 650
    GEPIKMEASS DGGFRSQENS PSPPRDGCKD GSPLAEPVEN GKELVKPLAS 700
    SLSGSTAIIT DHPPEQPFVN PLSALQSVMN IHLGKAAKPS LPALDPMSML 750
    FKMSNSLAEK AAVATPPPLQ SKKADHLDRY FYHVNNDQPI DLTKGKSDKG 800
    CSLGSVLLSP TSTAPATSSS TVTTAKTSAV VSFMSNSPLR ENALSDISDM 850
    LKNLTESHTS KSSTPSSISE KSDIDGATLE EAEESTPAQK RKGRQSNWNP 900
    QHLLILQAQF AASLRQTSEG KYIMSDLSPQ ERMHISRFTG LSMTTISHWL 950
    ANVKYQLRRT GGTKFLKNLD TGHPVFFCND CASQIRTPST YISHLESHLG 1000
    FRLRDLSKLS TEQINSQIAQ TKSPSEKMVT SSPEEDLGTS YQCKLCNRTF 1050
    ASKHAVKLHL SKTHGKSPED HLLYVSELEK Q 1081
    Length:1,081
    Mass (Da):118,566
    Last modified:January 10, 2006 - v2
    Checksum:iB4E0A4347B04E74A
    GO

    Sequence cautioni

    The sequence CAB66739.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti469 – 4691E → G.2 Publications
    Corresponds to variant rs143453460 [ dbSNP | Ensembl ].
    VAR_063635
    Natural varianti687 – 6871P → L.
    Corresponds to variant rs4805664 [ dbSNP | Ensembl ].
    VAR_052708

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX648745 mRNA. Translation: CAH56184.1.
    AB040907 mRNA. Translation: BAA95998.1.
    AL136805 mRNA. Translation: CAB66739.1. Different initiation.
    CCDSiCCDS12421.2.
    RefSeqiNP_065907.2. NM_020856.2.
    UniGeneiHs.278436.

    Genome annotation databases

    EnsembliENST00000240587; ENSP00000240587; ENSG00000121297.
    GeneIDi57616.
    KEGGihsa:57616.
    UCSCiuc002nsy.4. human.

    Polymorphism databases

    DMDMi85541971.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life's first breath - Issue 122 of October 2010

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX648745 mRNA. Translation: CAH56184.1 .
    AB040907 mRNA. Translation: BAA95998.1 .
    AL136805 mRNA. Translation: CAB66739.1 . Different initiation.
    CCDSi CCDS12421.2.
    RefSeqi NP_065907.2. NM_020856.2.
    UniGenei Hs.278436.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DMI NMR - A 202-303 [» ]
    ProteinModelPortali Q63HK5.
    SMRi Q63HK5. Positions 200-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121663. 3 interactions.
    IntActi Q63HK5. 1 interaction.
    MINTi MINT-4725267.
    STRINGi 9606.ENSP00000240587.

    PTM databases

    PhosphoSitei Q63HK5.

    Polymorphism databases

    DMDMi 85541971.

    Proteomic databases

    MaxQBi Q63HK5.
    PaxDbi Q63HK5.
    PRIDEi Q63HK5.

    Protocols and materials databases

    DNASUi 57616.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000240587 ; ENSP00000240587 ; ENSG00000121297 .
    GeneIDi 57616.
    KEGGi hsa:57616.
    UCSCi uc002nsy.4. human.

    Organism-specific databases

    CTDi 57616.
    GeneCardsi GC19M031765.
    HGNCi HGNC:30700. TSHZ3.
    HPAi HPA008834.
    MIMi 614119. gene.
    neXtProti NX_Q63HK5.
    PharmGKBi PA134887020.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG83709.
    HOGENOMi HOG000231480.
    HOVERGENi HBG079626.
    InParanoidi Q63HK5.
    KOi K09236.
    OMAi DPMSMLF.
    OrthoDBi EOG7NPFSF.
    PhylomeDBi Q63HK5.
    TreeFami TF328447.

    Miscellaneous databases

    ChiTaRSi TSHZ3. human.
    EvolutionaryTracei Q63HK5.
    GeneWikii TSHZ3.
    GenomeRNAii 57616.
    NextBioi 64282.
    PROi Q63HK5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q63HK5.
    Bgeei Q63HK5.
    CleanExi HS_TSHZ3.
    Genevestigatori Q63HK5.

    Family and domain databases

    InterProi IPR001356. Homeobox_dom.
    IPR027008. Teashirt_fam.
    IPR026810. Tshz3.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR12487. PTHR12487. 1 hit.
    PTHR12487:SF5. PTHR12487:SF5. 1 hit.
    SMARTi SM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 5 hits.
    [Graphical view ]
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-469.
      Tissue: Endometrial tumor.
    2. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1081.
      Tissue: Brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-1081.
      Tissue: Testis.
    4. "Teashirt 3 is necessary for ureteral smooth muscle differentiation downstream of SHH and BMP4."
      Caubit X., Lye C.M., Martin E., Core N., Long D.A., Vola C., Jenkins D., Garratt A.N., Skaer H., Woolf A.S., Fasano L.
      Development 135:3301-3310(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: FUNCTION, INTERACTION WITH APBB1; HDAC1 AND HDAC2, IDENTIFICATION IN A TRIMERIC COMPLEX WITH APBB1 AND HDAC1, CHROMATIN-BINDING, MUTAGENESIS OF 953-VAL--TYR-955, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric junction obstruction."
      Jenkins D., Caubit X., Dimovski A., Matevska N., Lye C.M., Cabuk F., Gucev Z., Tasic V., Fasano L., Woolf A.S.
      Nephrol. Dial. Transplant. 25:54-60(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT GLY-469, DEVELOPMENTAL STAGE.
    10. "Solution structure of the first and the second ZF-C2H2-like domains of human teashirt homolog 3."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 200-303.

    Entry informationi

    Entry nameiTSH3_HUMAN
    AccessioniPrimary (citable) accession number: Q63HK5
    Secondary accession number(s): Q9H0G6, Q9P254
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3