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Q63HK5 (TSH3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Teashirt homolog 3
Alternative name(s):
Zinc finger protein 537
Gene names
Name:TSHZ3
Synonyms:KIAA1474, TSH3, ZNF537
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1081 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter. Ref.6

Subunit structure

Interacts (via homeobox domain) with APBB1 (via PID domain 1). Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3. Ref.6

Subcellular location

Nucleus. Cell projectiongrowth cone By similarity. Note: Colocalizes with APBB1 in axonal growth cone By similarity. Colocalizes with APBB1 in the nucleus. Ref.6

Tissue specificity

Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease. Ref.4 Ref.6

Developmental stage

Expressed in peri-urothelial cells of the proximal ureter and renal pelvis at 9 weeks of gestation. Ref.9

Sequence similarities

Belongs to the teashirt C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence CAB66739.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCell projection
Nucleus
   Coding sequence diversityPolymorphism
   DomainCoiled coil
Homeobox
Repeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
Repressor
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processin utero embryonic development

Inferred from electronic annotation. Source: Ensembl

kidney morphogenesis

Inferred from electronic annotation. Source: Ensembl

kidney smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

lung development

Inferred from electronic annotation. Source: Ensembl

metanephros development

Inferred from electronic annotation. Source: Ensembl

musculoskeletal movement

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.6. Source: UniProtKB

positive regulation of smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

regulation of respiratory gaseous exchange by neurological system process

Inferred from sequence or structural similarity. Source: UniProtKB

sensory perception of touch

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ureter smooth muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

ureteric bud development

Inferred from electronic annotation. Source: Ensembl

ureteric peristalsis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentgrowth cone

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionchromatin binding

Inferred from direct assay Ref.6. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10811081Teashirt homolog 3
PRO_0000047066

Regions

Zinc finger214 – 23825C2H2-type 1
Zinc finger275 – 29925C2H2-type 2
Zinc finger386 – 40419C2H2-type 3; atypical
DNA binding891 – 96171Homeobox; atypical
Zinc finger976 – 99823C2H2-type 4
Zinc finger1041 – 106424C2H2-type 5
Coiled coil606 – 63025 Potential
Compositional bias142 – 16423Ser-rich
Compositional bias493 – 4964Poly-Glu

Amino acid modifications

Modified residue6821Phosphoserine Ref.8

Natural variations

Natural variant4691E → G. Ref.1 Ref.9
Corresponds to variant rs143453460 [ dbSNP | Ensembl ].
VAR_063635
Natural variant6871P → L.
Corresponds to variant rs4805664 [ dbSNP | Ensembl ].
VAR_052708

Experimental info

Mutagenesis953 – 9553VKY → ATA: Does not inhibit interaction with APBB1. Ref.6

Secondary structure

........... 1081
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q63HK5 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: B4E0A4347B04E74A

FASTA1,081118,566
        10         20         30         40         50         60 
MPRRKQQAPR RAAAYVSEEL KAAALVDEGL DPEEHTADGE PSAKYMCPEK ELARACPSYQ 

        70         80         90        100        110        120 
NSPAAEFSCH EMDSESHISE TSDRMADFES GSIKNEEETK EVTVPLEDTT VSDSLEQMKA 

       130        140        150        160        170        180 
VYNNFLSNSY WSNLNLNLHQ PSSEKNNGSS SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS 

       190        200        210        220        230        240 
QSRMLPEPSL FSTVQLYRQS SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR 

       250        260        270        280        290        300 
DDNHETDNNN PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY 

       310        320        330        340        350        360 
QKVPLKEPVT PVAAKIIPAT RKKASLELEL PSSPDSTGGT PKATISDTND ALQKNSNPYI 

       370        380        390        400        410        420 
TPNNRYGHQN GASYAWHFEA RKSQILKCME CGSSHDTLQE LTAHMMVTGH FIKVTNSAMK 

       430        440        450        460        470        480 
KGKPIVETPV TPTITTLLDE KVQSVPLAAT TFTSPSNTPA SISPKLNVEV KKEVDKEKAV 

       490        500        510        520        530        540 
TDEKPKQKDK PGEEEEKCDI SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG 

       550        560        570        580        590        600 
TPSWGGYPSI HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKNQT LVSPPSSQTS 

       610        620        630        640        650        660 
PMPKTNFHAM EELVKKVTEK VAKVEEKMKE PDGKLSPPKR ATPSPCSSEV GEPIKMEASS 

       670        680        690        700        710        720 
DGGFRSQENS PSPPRDGCKD GSPLAEPVEN GKELVKPLAS SLSGSTAIIT DHPPEQPFVN 

       730        740        750        760        770        780 
PLSALQSVMN IHLGKAAKPS LPALDPMSML FKMSNSLAEK AAVATPPPLQ SKKADHLDRY 

       790        800        810        820        830        840 
FYHVNNDQPI DLTKGKSDKG CSLGSVLLSP TSTAPATSSS TVTTAKTSAV VSFMSNSPLR 

       850        860        870        880        890        900 
ENALSDISDM LKNLTESHTS KSSTPSSISE KSDIDGATLE EAEESTPAQK RKGRQSNWNP 

       910        920        930        940        950        960 
QHLLILQAQF AASLRQTSEG KYIMSDLSPQ ERMHISRFTG LSMTTISHWL ANVKYQLRRT 

       970        980        990       1000       1010       1020 
GGTKFLKNLD TGHPVFFCND CASQIRTPST YISHLESHLG FRLRDLSKLS TEQINSQIAQ 

      1030       1040       1050       1060       1070       1080 
TKSPSEKMVT SSPEEDLGTS YQCKLCNRTF ASKHAVKLHL SKTHGKSPED HLLYVSELEK 


Q 

« Hide

References

« Hide 'large scale' references
[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-469.
Tissue: Endometrial tumor.
[2]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1081.
Tissue: Brain.
[3]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-1081.
Tissue: Testis.
[4]"Teashirt 3 is necessary for ureteral smooth muscle differentiation downstream of SHH and BMP4."
Caubit X., Lye C.M., Martin E., Core N., Long D.A., Vola C., Jenkins D., Garratt A.N., Skaer H., Woolf A.S., Fasano L.
Development 135:3301-3310(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4."
Kajiwara Y., Akram A., Katsel P., Haroutunian V., Schmeidler J., Beecham G., Haines J.L., Pericak-Vance M.A., Buxbaum J.D.
PLoS ONE 4:E5071-E5071(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH APBB1; HDAC1 AND HDAC2, IDENTIFICATION IN A TRIMERIC COMPLEX WITH APBB1 AND HDAC1, CHROMATIN-BINDING, MUTAGENESIS OF 953-VAL--TYR-955, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric junction obstruction."
Jenkins D., Caubit X., Dimovski A., Matevska N., Lye C.M., Cabuk F., Gucev Z., Tasic V., Fasano L., Woolf A.S.
Nephrol. Dial. Transplant. 25:54-60(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT GLY-469, DEVELOPMENTAL STAGE.
[10]"Solution structure of the first and the second ZF-C2H2-like domains of human teashirt homolog 3."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 200-303.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Life's first breath - Issue 122 of October 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX648745 mRNA. Translation: CAH56184.1.
AB040907 mRNA. Translation: BAA95998.1.
AL136805 mRNA. Translation: CAB66739.1. Different initiation.
CCDSCCDS12421.2.
RefSeqNP_065907.2. NM_020856.2.
UniGeneHs.278436.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMINMR-A202-303[»]
ProteinModelPortalQ63HK5.
SMRQ63HK5. Positions 200-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121663. 3 interactions.
IntActQ63HK5. 1 interaction.
MINTMINT-4725267.
STRING9606.ENSP00000240587.

PTM databases

PhosphoSiteQ63HK5.

Polymorphism databases

DMDM85541971.

Proteomic databases

MaxQBQ63HK5.
PaxDbQ63HK5.
PRIDEQ63HK5.

Protocols and materials databases

DNASU57616.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000240587; ENSP00000240587; ENSG00000121297.
GeneID57616.
KEGGhsa:57616.
UCSCuc002nsy.4. human.

Organism-specific databases

CTD57616.
GeneCardsGC19M031765.
HGNCHGNC:30700. TSHZ3.
HPAHPA008834.
MIM614119. gene.
neXtProtNX_Q63HK5.
PharmGKBPA134887020.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG83709.
HOGENOMHOG000231480.
HOVERGENHBG079626.
InParanoidQ63HK5.
KOK09236.
OMADPMSMLF.
OrthoDBEOG7NPFSF.
PhylomeDBQ63HK5.
TreeFamTF328447.

Gene expression databases

ArrayExpressQ63HK5.
BgeeQ63HK5.
CleanExHS_TSHZ3.
GenevestigatorQ63HK5.

Family and domain databases

InterProIPR001356. Homeobox_dom.
IPR027008. Teashirt_fam.
IPR026810. Tshz3.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERPTHR12487. PTHR12487. 1 hit.
PTHR12487:SF5. PTHR12487:SF5. 1 hit.
SMARTSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTSHZ3. human.
EvolutionaryTraceQ63HK5.
GeneWikiTSHZ3.
GenomeRNAi57616.
NextBio64282.
PROQ63HK5.
SOURCESearch...

Entry information

Entry nameTSH3_HUMAN
AccessionPrimary (citable) accession number: Q63HK5
Secondary accession number(s): Q9H0G6, Q9P254
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM