Reviewed, UniProtKB/Swiss-Prot Q63GW2 (GCP_BACCZ)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BCE33L0235

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303265
Sites
	Metal binding	120	1	Zinc Potential
	Metal binding	124	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q63GW2-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E793A7F804FA8CA4	FASTA	343	36,567
	10 20 30 40 50 60 MGEYIMEKNT IILGIETSCD ETAVAVVKNG TEIIANVVAS QIESHKRFGG VVPEIASRHH 70 80 90 100 110 120 VEEITVVLEE ALKEANITFD DIDAIAVTEG PGLVGALLIG VNAAKAVAFA HDIPLVGVHH 130 140 150 160 170 180 IAGHIYANRL VKEVQFPLLS LVVSGGHTEL VYMKEHGSFE VIGETRDDAA GEAYDKVART 190 200 210 220 230 240 LSMPYPGGPH IDRLAHEGKP TIDLPRAWLE PDSYDFSFSG LKSAVINTVH NAKQRGIEIA 250 260 270 280 290 300 PEDLAASFQE SVIDVLVTKA SRAADAYNVK QVLLAGGVAA NKGLRARLET EFAQKENVEL 310 320 330 340 IIPPLSLCTD NAAMIAAAGT IAYEQGKRAT LALNANPGLD IEA

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU19999.1.
RefSeq	YP_081849.2.
3D structure databases
SMR	Q63GW2. Positions 11-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63GW2.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	3022252.
GenomeReviews	Gene locus BCE33L0235 in contig CP000001_GR.
KEGG	bcz:BCZK0235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0533.
HOGENOM	HBG304663.
OMA	CKRALKQ.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L0235-MONOMER.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BACCZ

Accession

Primary (citable) accession number: Q63GW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)