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Q63GK5

- TOP3_BACCZ

UniProt

Q63GK5 - TOP3_BACCZ

Protein

DNA topoisomerase 3

Gene

topB

Organism
Bacillus cereus (strain ZK / E33L)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium; catalyticUniRule annotation
    Sitei61 – 611Interaction with DNAUniRule annotation
    Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
    Sitei168 – 1681Interaction with DNAUniRule annotation
    Sitei176 – 1761Interaction with DNAUniRule annotation
    Active sitei310 – 3101O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei312 – 3121Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBCER288681:GHG7-395-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IIIUniRule annotation
    Gene namesi
    Name:topBUniRule annotation
    Ordered Locus Names:BCE33L0347
    OrganismiBacillus cereus (strain ZK / E33L)
    Taxonomic identifieri288681 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000002612: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729DNA topoisomerase 3PRO_0000286362Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi288681.BCZK0347.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63GK5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 136134ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni187 – 1926Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0551.
    KOiK03169.
    OMAiTKHDEYF.
    OrthoDBiEOG60CWM6.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00953. Topoisom_3_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01056. topB. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63GK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKSVVIAEK PSVARDIARV LKCDKKGNGY LEGSKYIVTW ALGHLVTLAD    50
    PESYDVKYKK WNLEDLPMLP ERLKLTVIKQ TGKQFNAVKS QLLRKDVNEI 100
    IVATDAGREG ELVARWIIDK VRINKPIKRL WISSVTDKAI KDGFANLKPG 150
    KAYDNLYASA VARSEADWYI GLNATRALTT RFNAQLNCGR VQTPTVAMIA 200
    NREDEIKNFK AQTYYGIEAQ TTNQLKLTWQ DANGNSRSFN KEKIDGIVKG 250
    LDKHNATVLE IDKKQKKSFS PGLYDLTELQ RDANKKFGYS AKETLNIMQK 300
    LYEQHKVLTY PRTDSRYISS DIVGTLPERL KACGVGEYRP LAHKVLQKPI 350
    KANKSFVDDS KVSDHHAIIP TEGYVNFSAF TDKERKIYDL VVKRFLAVLF 400
    PAFEYEQLTL RTKIGNETFI ARGKTILHAG WKEVYENRFE DDDVTDEVKE 450
    QLLPRIEKGD TLTVKLIMQT SGQTKAPARF NEATLLSAME NPTKYMDTQN 500
    KQLADTLKST GGLGTVATRA DIIDKLFNSF LIEKRGKDIH ITSKGRQLLD 550
    LVPEELKSPT LTGEWEQKLE AIAKGKLKKE VFISEMKNYT KEIVSEIKSS 600
    DKKYKHDNIS TKSCPDCGKP MLEVNGKKGK MLVCQDRECG HRKNVSRTTN 650
    ARCPQCKKKL ELRGEGAGQI FACKCGYREK LSTFQERRKK ESGNKADKRD 700
    VQKYMKQQKK EEEPLNNPFA EALKKLKFD 729
    Length:729
    Mass (Da):82,840
    Last modified:October 25, 2004 - v1
    Checksum:iA7AD741BBD75CC1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000001 Genomic DNA. Translation: AAU19892.1.
    RefSeqiWP_001140208.1. NC_006274.1.
    YP_081956.1. NC_006274.1.

    Genome annotation databases

    EnsemblBacteriaiAAU19892; AAU19892; BCE33L0347.
    GeneIDi3023182.
    KEGGibcz:BCZK0347.
    PATRICi18883976. VBIBacCer95304_0370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000001 Genomic DNA. Translation: AAU19892.1 .
    RefSeqi WP_001140208.1. NC_006274.1.
    YP_081956.1. NC_006274.1.

    3D structure databases

    ProteinModelPortali Q63GK5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 288681.BCZK0347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU19892 ; AAU19892 ; BCE33L0347 .
    GeneIDi 3023182.
    KEGGi bcz:BCZK0347.
    PATRICi 18883976. VBIBacCer95304_0370.

    Phylogenomic databases

    eggNOGi COG0551.
    KOi K03169.
    OMAi TKHDEYF.
    OrthoDBi EOG60CWM6.

    Enzyme and pathway databases

    BioCyci BCER288681:GHG7-395-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00953. Topoisom_3_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005738. TopoIII.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01056. topB. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ZK / E33L.

    Entry informationi

    Entry nameiTOP3_BACCZ
    AccessioniPrimary (citable) accession number: Q63GK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3