Q63GI2 (SYP2_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase 2
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase 2
Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	BCE33L0370

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.
Sequence caution	The sequence AAU19870.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Proline--tRNA ligase 2 HAMAP MF_01571		PRO_0000249119

Sequences

	Sequence		Length	Mass (Da)
	Q63GI2 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: C2697C255B30FD93	FASTA	476	54,665
	10 20 30 40 50 60 MAKEQVQAIT KMEEDFAQWY TDIVKKAELV DYSSVKGCMI LRPYGYALWE NMQKVMDEKL 70 80 90 100 110 120 KATGHENVYM PMFIPESLLQ KEKDHVEGFA PEVAWVTHGG DEKLAERLCI RPTSETLFCE 130 140 150 160 170 180 HFSKIVQSYN DLPKLYNQWC SVVRWEKTTR PFLRTTEFLW QEGHTIHETA EESQAETLNI 190 200 210 220 230 240 LNLYASFCED YLAIPVIKGQ KTEKEKFAGA KATYTIESLM HDGKALQTGT SHNFGTNFSE 250 260 270 280 290 300 AFDIKFLDRT GKWQYVHQTS WGVSTRMIGG LIMVHGDNNG LVMPPKVAPV QVVIVPIAQH 310 320 330 340 350 360 KEGVLAKAIE LQGHIQKVAR VKIDASNKTP GWKFNEYEMK GIPIRLEVGP KDIEKNQVVL 370 380 390 400 410 420 VRRDTKEKEF ISMDQLEERI PALLEEIHNS LFNKAKVFRD ENTYSVTNFE EMKKIADEKQ 430 440 450 460 470 GFIKAMWCGE LACEEKLKEE VGVSSRCMPF EQEHLADECV CCGKEAKQMV YWGKAY

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU19870.1. Different initiation.
RefSeq	YP_081979.2. NC_006274.1.
3D structure databases
HSSP	HSSP built from PDB template 1NJ2 based on UniProtKB O26708.
ProteinModelPortal	Q63GI2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63GI2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000044184; EBBACP00000043085; EBBACG00000044175.
GeneID	3025170.
GenomeReviews	Gene locus BCE33L0370 in contig CP000001_GR.
KEGG	bcz:BCZK0370.
PATRIC	18884022. VBIBacCer95304_0393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000001167.
HOGENOM	HBG334108.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L0370-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_BACCZ

Accession

Primary (citable) accession number: Q63GI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program