Q63EX2 (GLPK_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BCE33L0938

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycerol kinase HAMAP MF_00186		PRO_1000020700
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63EX2 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 88359600B5C05417	FASTA	496	55,014
	10 20 30 40 50 60 MKKYILSLDQ GTTSSRAILF NKKGEIVHSA QKEFTQHFPK PGWVEHNAQE IWGSILAVIA 70 80 90 100 110 120 TCLSEADVKP EQIAGIGITN QRETAVVWDK TTGKPIYNAI VWQSRQTAEI CDELKEKGYG 130 140 150 160 170 180 EMVREKTGLL IDAYFSGTKV KWILDNVEGA REKAENGDLL FGTIDTWLVW KLSGGKAHVT 190 200 210 220 230 240 DYSNASRTLM FNIHDLQWDD ELLDMLTVPK SMLPEVRPSS EVYGETIDYH FFGQNVPIAG 250 260 270 280 290 300 VAGDQQAALF GQACFGEGMA KNTYGTGCFM LMNTGEKAVA SEHGLLTTIA WGIDGKVNYA 310 320 330 340 350 360 LEGSIFVAGS AIQWLRDGMR MFKDASESEV YASRVESTDG VYVVPAFVGL GTPYWDSEVR 370 380 390 400 410 420 GAMFGVTRGT TKEHFIRATL ESLAYQTKDV LCAMEADSGI ELKTLRVDGG AVKNNFLMKF 430 440 450 460 470 480 QSDILDVPVE RPVINETTAL GAAYLAGLAV GYWKNQDEIK EQWHMDKRFE PTMEAETSEE 490 LYAGWKKAIE ATKAFK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU19307.1.
RefSeq	YP_082540.1. NC_006274.1.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P0A6F3.
ProteinModelPortal	Q63EX2.
SMR	Q63EX2. Positions 4-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63EX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000045047; EBBACP00000043948; EBBACG00000045038.
GeneID	3022923.
GenomeReviews	Gene locus BCE33L0938 in contig CP000001_GR.
KEGG	bcz:BCZK0938.
PATRIC	18885290. VBIBacCer95304_0985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
GeneTree	EBGT00050000001374.
HOGENOM	HBG511469.
OMA	ALYGQLC.
PhylomeDB	Q63EX2.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L0938-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BACCZ

Accession

Primary (citable) accession number: Q63EX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program