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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Bacillus cereus (strain ZK / E33L)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciBCER288681:G1G3U-1490-MONOMER
UniPathwayiUPA00048; UER00070

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:BCE33L1285
OrganismiBacillus cereus (strain ZK / E33L)
Taxonomic identifieri288681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002612 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001491341 – 5062-isopropylmalate synthaseAdd BLAST506

Proteomic databases

PRIDEiQ63DX8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ63DX8
SMRiQ63DX8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 266Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST263

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

KOiK01649
OMAiKFHGVGL

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01025 LeuA_type1, 1 hit
InterProiView protein in InterPro
IPR013709 2-isopropylmalate_synth_dimer
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR036230 LeuA_allosteric_dom_sf
IPR005671 LeuA_bact_synth
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
PF08502 LeuA_dimer, 1 hit
SMARTiView protein in SMART
SM00917 LeuA_dimer, 1 hit
SUPFAMiSSF110921 SSF110921, 1 hit
TIGRFAMsiTIGR00973 leuA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

Q63DX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKILFMDTT LRDGEQSPGV NLNEQEKLQI ARQLERLGIH VMEAGFAAAS
60 70 80 90 100
EGDFQSVKRI ANTIQNATVM SLARAKESDI RRAYEAVKGA VSPRLHVFLA
110 120 130 140 150
TSDIHMKYKL CMSKEDVLDS IYRSVTLGKS LFPTVQFSAE DATRTARDFL
160 170 180 190 200
AEAVEVAIRA GANVINIPDT VGYTNPEEYY SLFKYLQESV PSYEKAIFSC
210 220 230 240 250
HCHDDLGMAV ANSLAAVEGG ALQVEGTING IGERAGNAAL EEVAVALHIR
260 270 280 290 300
KDFYKAEPSM TLKEIKATST LVSRLTGMVV PKNKAIVGAN AFAHESGIHQ
310 320 330 340 350
DGVLKEVTTY EIIEPALVGE SQNLFVLGKH SGRHAFTEKM KELGYEFTND
360 370 380 390 400
ERDAVFEAFK KLADRKKEIT EEDLRALMLG EAAFAAQQYN ITQLQVHFVS
410 420 430 440 450
NSTQCATVVL KDEEGNVFED AATGSGSIEA IYNAIQRILG LECELADYRI
460 470 480 490 500
QSITQGQDAL AHVHVELKEG AHQVSGFGVA QDVLEASARA YVHAAGKLKS

FIQLVK
Length:506
Mass (Da):55,401
Last modified:October 25, 2004 - v1
Checksum:i4AB125DDC8596189
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000001 Genomic DNA Translation: AAU18963.1
RefSeqiWP_000721813.1, NZ_CP009968.1

Genome annotation databases

EnsemblBacteriaiAAU18963; AAU18963; BCE33L1285
KEGGibcz:BCE33L1285
PATRICifig|288681.22.peg.4269

Similar proteinsi

Entry informationi

Entry nameiLEU1_BACCZ
AccessioniPrimary (citable) accession number: Q63DX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health