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Q63DX8 (LEU1_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase

EC=2.3.3.13
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
Gene names
Name:leuA
Ordered Locus Names:BCE33L1285
OrganismBacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]
Taxonomic identifier288681 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01025

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function2-isopropylmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5065062-isopropylmalate synthase HAMAP-Rule MF_01025
PRO_1000149134

Sequences

Sequence LengthMass (Da)Tools
Q63DX8 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 4AB125DDC8596189

FASTA50655,401
        10         20         30         40         50         60 
MKKILFMDTT LRDGEQSPGV NLNEQEKLQI ARQLERLGIH VMEAGFAAAS EGDFQSVKRI 

        70         80         90        100        110        120 
ANTIQNATVM SLARAKESDI RRAYEAVKGA VSPRLHVFLA TSDIHMKYKL CMSKEDVLDS 

       130        140        150        160        170        180 
IYRSVTLGKS LFPTVQFSAE DATRTARDFL AEAVEVAIRA GANVINIPDT VGYTNPEEYY 

       190        200        210        220        230        240 
SLFKYLQESV PSYEKAIFSC HCHDDLGMAV ANSLAAVEGG ALQVEGTING IGERAGNAAL 

       250        260        270        280        290        300 
EEVAVALHIR KDFYKAEPSM TLKEIKATST LVSRLTGMVV PKNKAIVGAN AFAHESGIHQ 

       310        320        330        340        350        360 
DGVLKEVTTY EIIEPALVGE SQNLFVLGKH SGRHAFTEKM KELGYEFTND ERDAVFEAFK 

       370        380        390        400        410        420 
KLADRKKEIT EEDLRALMLG EAAFAAQQYN ITQLQVHFVS NSTQCATVVL KDEEGNVFED 

       430        440        450        460        470        480 
AATGSGSIEA IYNAIQRILG LECELADYRI QSITQGQDAL AHVHVELKEG AHQVSGFGVA 

       490        500 
QDVLEASARA YVHAAGKLKS FIQLVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000001 Genomic DNA. Translation: AAU18963.1.
RefSeqYP_082884.1. NC_006274.1.

3D structure databases

ProteinModelPortalQ63DX8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288681.BCZK1285.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU18963; AAU18963; BCE33L1285.
GeneID3023896.
KEGGbcz:BCZK1285.
PATRIC18886034. VBIBacCer95304_1356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
KOK01649.
OMARKINIFD.
OrthoDBEOG6CGCF3.

Enzyme and pathway databases

BioCycBCER288681:GHG7-1366-MONOMER.
UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01025. LeuA_type1.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. SSF110921. 1 hit.
TIGRFAMsTIGR00973. leuA_bact. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU1_BACCZ
AccessionPrimary (citable) accession number: Q63DX8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways