Q63D03 (ASNA_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	BCE33L1620

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017935

Sequences

	Sequence		Length	Mass (Da)
	Q63D03 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 38CAB10CC09A370A	FASTA	327	38,087
	10 20 30 40 50 60 MYQSLMTVRE TQIAIKEVKT FFEDQLAKRL ELFRVSAPLF VTKKSGLNDH LNGVERPIEF 70 80 90 100 110 120 DMLHSGEELE IVHSLAKWKR FALHEYGYEA GEGLYTNMNA IRRDEELDAT HSIYVDQWDW 130 140 150 160 170 180 EKIVQKEWRT VDYLQKTVLT IYGIFKDLED HLFEKYPFLG KYLPEEIVFV TSQELEDKYP 190 200 210 220 230 240 ELTPKDREHA IAKEHGAVFI IGIGDALRSG EKHDGRAADY DDWKLNGDIL FWHPVLQSSF 250 260 270 280 290 300 ELSSMGIRVD SKSLDEQLTK TGEDFKREYD FHKGILEDVL PLTIGGGIGQ SRMCMYFLRK 310 320 AHIGEVQSSV WPDDLREACK KENIHLF

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU18633.1.
RefSeq	YP_083214.1. NC_006274.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
ProteinModelPortal	Q63D03.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63D03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000042354; EBBACP00000041255; EBBACG00000042345.
GeneID	3025576.
GenomeReviews	Gene locus BCE33L1620 in contig CP000001_GR.
KEGG	bcz:BCZK1620.
PATRIC	18886726. VBIBacCer95304_1702.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
GeneTree	EBGT00050000003597.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L1620-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_BACCZ

Accession

Primary (citable) accession number: Q63D03

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program