Reviewed, UniProtKB/Swiss-Prot Q63CG2 (NADE_BACCZ)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BCE33L1810

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077535
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63CG2-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: B58A9792E8075B98	FASTA	272	30,101
	10 20 30 40 50 60 MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 130 140 150 160 170 180 NLLDESLTDF NKGNVKARIR MVTQYAIGGQ KGLLVIGTDH AAEAVTGFFT KFGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RALLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKT 250 260 270 VPADVAEKIE KRYTVSEHKR QVPASMFDDW WK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000001 Genomic DNA. Translation: AAU18443.1.
RefSeq	YP_083405.1.
3D structure databases
SMR	Q63CG2. Positions 2-272.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63CG2.
Genome annotation databases
GeneID	3024423.
GenomeReviews	Gene locus BCE33L1810 in contig CP000001_GR.
KEGG	bcz:BCZK1810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63CG2.
OMA	ADLEEDR.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L1810-MON.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACCZ

Accession

Primary (citable) accession number: Q63CG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)